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<t>AdipoRon</t> alleviated lipid metabolism disorders and cellular lipotoxic injury induced by mixed fatty acids (OA + PA) in LMH cells. (A) Effects of different concentrations of AdipoRon on LMH <t>cell</t> <t>viability.</t> (B) Effects of AdipoRon intervention on lipid deposition in mixed fatty acid (OA + PA)-treated LMH cells (Oil Red O staining). (C) Effects of AdipoRon intervention on TG and TC levels in mixed fatty acid (OA + PA)-treated LMH cells. (D) Effects of AdipoRon intervention on the activities of GPT and GOT in the culture medium of mixed fatty acid (OA + PA)-treated LMH cells. Abbreviations: C, control group; H, mixed fatty acid (0.30 mM OA + 0.15 mM PA)-treated group; H+LA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 20 μM AdipoRon-treated group; H+MA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 40 μM AdipoRon-treated group; H+HA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 60 μM AdipoRon-treated group; OA, oleic acid; PA, palmitic acid; TG, triglyceride; TC, total cholesterol; GPT, glutamic pyruvic transaminase; GOT, glutamic oxaloacetic transaminase. All the data are expressed as the mean±SEM. * indicates P <0.05; ** indicates P <0.01; *** indicates P <0.001; ⁎⁎⁎⁎ indicates P <0.0001.
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<t>AdipoRon</t> alleviated lipid metabolism disorders and cellular lipotoxic injury induced by mixed fatty acids (OA + PA) in LMH cells. (A) Effects of different concentrations of AdipoRon on LMH <t>cell</t> <t>viability.</t> (B) Effects of AdipoRon intervention on lipid deposition in mixed fatty acid (OA + PA)-treated LMH cells (Oil Red O staining). (C) Effects of AdipoRon intervention on TG and TC levels in mixed fatty acid (OA + PA)-treated LMH cells. (D) Effects of AdipoRon intervention on the activities of GPT and GOT in the culture medium of mixed fatty acid (OA + PA)-treated LMH cells. Abbreviations: C, control group; H, mixed fatty acid (0.30 mM OA + 0.15 mM PA)-treated group; H+LA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 20 μM AdipoRon-treated group; H+MA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 40 μM AdipoRon-treated group; H+HA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 60 μM AdipoRon-treated group; OA, oleic acid; PA, palmitic acid; TG, triglyceride; TC, total cholesterol; GPT, glutamic pyruvic transaminase; GOT, glutamic oxaloacetic transaminase. All the data are expressed as the mean±SEM. * indicates P <0.05; ** indicates P <0.01; *** indicates P <0.001; ⁎⁎⁎⁎ indicates P <0.0001.
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AdipoRon alleviated lipid metabolism disorders and cellular lipotoxic injury induced by mixed fatty acids (OA + PA) in LMH cells. (A) Effects of different concentrations of AdipoRon on LMH cell viability. (B) Effects of AdipoRon intervention on lipid deposition in mixed fatty acid (OA + PA)-treated LMH cells (Oil Red O staining). (C) Effects of AdipoRon intervention on TG and TC levels in mixed fatty acid (OA + PA)-treated LMH cells. (D) Effects of AdipoRon intervention on the activities of GPT and GOT in the culture medium of mixed fatty acid (OA + PA)-treated LMH cells. Abbreviations: C, control group; H, mixed fatty acid (0.30 mM OA + 0.15 mM PA)-treated group; H+LA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 20 μM AdipoRon-treated group; H+MA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 40 μM AdipoRon-treated group; H+HA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 60 μM AdipoRon-treated group; OA, oleic acid; PA, palmitic acid; TG, triglyceride; TC, total cholesterol; GPT, glutamic pyruvic transaminase; GOT, glutamic oxaloacetic transaminase. All the data are expressed as the mean±SEM. * indicates P <0.05; ** indicates P <0.01; *** indicates P <0.001; ⁎⁎⁎⁎ indicates P <0.0001.

Journal: Poultry Science

Article Title: The adiponectin receptor agonist AdipoRon alleviates lipotoxic injury in LMH cells

doi: 10.1016/j.psj.2026.106573

Figure Lengend Snippet: AdipoRon alleviated lipid metabolism disorders and cellular lipotoxic injury induced by mixed fatty acids (OA + PA) in LMH cells. (A) Effects of different concentrations of AdipoRon on LMH cell viability. (B) Effects of AdipoRon intervention on lipid deposition in mixed fatty acid (OA + PA)-treated LMH cells (Oil Red O staining). (C) Effects of AdipoRon intervention on TG and TC levels in mixed fatty acid (OA + PA)-treated LMH cells. (D) Effects of AdipoRon intervention on the activities of GPT and GOT in the culture medium of mixed fatty acid (OA + PA)-treated LMH cells. Abbreviations: C, control group; H, mixed fatty acid (0.30 mM OA + 0.15 mM PA)-treated group; H+LA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 20 μM AdipoRon-treated group; H+MA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 40 μM AdipoRon-treated group; H+HA, mixed fatty acid (0.30 mM OA + 0.15 mM PA) + 60 μM AdipoRon-treated group; OA, oleic acid; PA, palmitic acid; TG, triglyceride; TC, total cholesterol; GPT, glutamic pyruvic transaminase; GOT, glutamic oxaloacetic transaminase. All the data are expressed as the mean±SEM. * indicates P <0.05; ** indicates P <0.01; *** indicates P <0.001; ⁎⁎⁎⁎ indicates P <0.0001.

Article Snippet: To evaluate the effects of AdipoRon on cell viability, LMH cells were treated with different concentrations (0, 10, 15, 20, 25, 30, 40, 50, 60, or 70 μM) of AdipoRon (Cat# HY-15848; MedChemExpress, Shanghai, China) for 24 h, and cell viability was determined following the instructions for the CCK-8 assay kit (Cat# E-CK-A362; Elabscience, Wuhan, China).

Techniques: Staining, Control