Journal: International Journal of Molecular Sciences

Article Title: Dynamics of Chromatin Opening across Larval Development in the Urochordate Ascidian Ciona savignyi

doi: 10.3390/ijms25052793

Figure Lengend Snippet: Expression and functional assay of key genes in larval development. ( A ) Tissue expression patterns of the selected genes as determined by promoter assay. Scale bar, 50 μm. ( B ) Treatment with an MMP24 inhibitor (GM6001, 40 μM) arrested lumen expansion. Scale bar, 10 μm. The asterisk marks the lumen. ( C ) Quantification of lumen volume in DMSO- (n = 191) and GM6001-treated embryos (n = 165) embryos at 22 hpf. Statistical analysis was performed using Student’s t -test, p < 0.001 (***). ( D ) Overexpression of dominant-negative Cs -KLF5 led to failure of lumen formation. Schematic images located on the right side of confocal images present the phenotypes of Cs -KLF5 knockdown and control. The green, blue, and red areas represent the notochord cells, nuclei, and nuclei in cells expressing Bra > Cs -KLF5DN::tdtomato/ Bra > tdtomato (red), respectively. Scale bar, 10 μm.

Article Snippet: Embryos were treated with 40 µM of the inhibitors ilomastat (GM6001, HY-15768, MedChemExpres, Monmouth Junction, NJ, USA) [ ] and DMSO at 12 h post fertilization (hpf).

Techniques: Expressing, Functional Assay, Promoter Assay, Over Expression, Dominant Negative Mutation, Knockdown, Control