b longum atcc 15707  (ATCC)


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    ATCC b longum atcc 15707
    List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .
    B Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Comparison of fidaxomicin, thuricin CD, vancomycin and nisin highlights the narrow spectrum nature of thuricin CD"

    Article Title: Comparison of fidaxomicin, thuricin CD, vancomycin and nisin highlights the narrow spectrum nature of thuricin CD

    Journal: Gut Microbes

    doi: 10.1080/19490976.2024.2342583

    List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .
    Figure Legend Snippet: List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .

    Techniques Used:

    bifidobacterium longum subsp longum atcc 15707  (ATCC)


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    ATCC bifidobacterium longum subsp longum atcc 15707
    Bifidobacterium Longum Subsp Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bifidobacterium longum atcc 15707  (ATCC)


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    ATCC bifidobacterium longum atcc 15707
    Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    control strain bifidobacterium longum atcc 15707  (ATCC)


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    ATCC control strain bifidobacterium longum atcc 15707
    MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.
    Control Strain Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles"

    Article Title: Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles

    Journal: bioRxiv

    doi: 10.1101/2024.02.12.579962


    Figure Legend Snippet: MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.

    Techniques Used: Derivative Assay

    The ability of B. longum strains to stimulate growth of L. reuteri DSM 17938 by co-cultivation in SIM was tested. 4, 10 and 25% of bifidobacterial cell suspension was added and the L. reuteri DSM 17938 cultures were grown for 48 h followed by OD 600 measurement. Significance level used was *p < 0.05.
    Figure Legend Snippet: The ability of B. longum strains to stimulate growth of L. reuteri DSM 17938 by co-cultivation in SIM was tested. 4, 10 and 25% of bifidobacterial cell suspension was added and the L. reuteri DSM 17938 cultures were grown for 48 h followed by OD 600 measurement. Significance level used was *p < 0.05.

    Techniques Used: Suspension

    Physiological characterization of the B. longum strains. Scanning electron microscope images showing the morphology of BG-L47 (A) and BG-L48 (B) . The samples were sputtered with gold before imaging. Adhesion of the B. longum strains to mucus (C) . After allowing the strains to adhere to mucus-coated microtiter wells, the numbers of bacteria was determined by trypsin release and plating. Detection limit marked with a dotted red line. Bile tolerance of the B. longum strains (D) was determined by exposing the bacteria to 0.3% bovine bile for 90 minutes, after which CFU was measured. Low pH survival of the B. longum strains (E) was assessed by exposing the bacteria to synthetic gastric juice at pH 3.0. CFU was measured after 0, 20, 50 and 90 minutes of exposure. The significance level for all statistical analyzes was *p < 0.05.
    Figure Legend Snippet: Physiological characterization of the B. longum strains. Scanning electron microscope images showing the morphology of BG-L47 (A) and BG-L48 (B) . The samples were sputtered with gold before imaging. Adhesion of the B. longum strains to mucus (C) . After allowing the strains to adhere to mucus-coated microtiter wells, the numbers of bacteria was determined by trypsin release and plating. Detection limit marked with a dotted red line. Bile tolerance of the B. longum strains (D) was determined by exposing the bacteria to 0.3% bovine bile for 90 minutes, after which CFU was measured. Low pH survival of the B. longum strains (E) was assessed by exposing the bacteria to synthetic gastric juice at pH 3.0. CFU was measured after 0, 20, 50 and 90 minutes of exposure. The significance level for all statistical analyzes was *p < 0.05.

    Techniques Used: Microscopy, Imaging, Bacteria

    (A) Glycoside hydrolases (GH) in B. longum BG-L47 and 4 control strains. The GHs are sorted into four categories according to the origin of the substrate: Human milk glycans, host glycans, dietary glycans and microbially derived glycans. The number of genes within a particular GH family is illustrated by a color scheme representing 0-5. (B) Fiber utilization in BG-L47 and BB536 presented together with GHs predicted to be involved in utilization of that specific fiber. Growth was determined by measuring the pH drop when cultured in mMRS with the fibers as carbon source. The explanation of the scale is 0= pH drop 0-0.1, 1= pH drop 0.2-0.5, 2= pH drop 0.5-1, 3= pH drop 1-1.5. (C) Predicted extracellular localization of BG-L47 glycoside hydrolases. The protein sequences were analyzed with SignalP, TMHMM, BLAST and subjected to manual annotation; The method for classification of cell surface localization described by Båth et al., 2005 was used.
    Figure Legend Snippet: (A) Glycoside hydrolases (GH) in B. longum BG-L47 and 4 control strains. The GHs are sorted into four categories according to the origin of the substrate: Human milk glycans, host glycans, dietary glycans and microbially derived glycans. The number of genes within a particular GH family is illustrated by a color scheme representing 0-5. (B) Fiber utilization in BG-L47 and BB536 presented together with GHs predicted to be involved in utilization of that specific fiber. Growth was determined by measuring the pH drop when cultured in mMRS with the fibers as carbon source. The explanation of the scale is 0= pH drop 0-0.1, 1= pH drop 0.2-0.5, 2= pH drop 0.5-1, 3= pH drop 1-1.5. (C) Predicted extracellular localization of BG-L47 glycoside hydrolases. The protein sequences were analyzed with SignalP, TMHMM, BLAST and subjected to manual annotation; The method for classification of cell surface localization described by Båth et al., 2005 was used.

    Techniques Used: Derivative Assay, Cell Culture

    Bioactivities of MV isolated from L. reuteri DSM 17938 co-cultured with B. longum BG-L47 (MV 47 ), BB536 (MV 536 ), or grown in SIM with fructose as electron acceptor (MV u ) (A) Protein concentrations of MV preparations. Data are presented as boxplots with min to max (n=3). (B) 5’NT activity of MV preparations. Values are presented as means with SD (n=5). (C) TRPV1 antagonizing effects of MV preparations in a rat dorsal root ganglion (DRG) model. Vesicles were incubated with DRGs, and calcium flux were monitored after addition of the agonist capsaicin, n=6. Boxplots with min to max and average value representation. (D) and (E) MV stimulation of IL-6 and IL-1ß secretion in PBMCs from healthy donors measured by ELISA (n=6). The significance level in all graphs is *p < 0.05.
    Figure Legend Snippet: Bioactivities of MV isolated from L. reuteri DSM 17938 co-cultured with B. longum BG-L47 (MV 47 ), BB536 (MV 536 ), or grown in SIM with fructose as electron acceptor (MV u ) (A) Protein concentrations of MV preparations. Data are presented as boxplots with min to max (n=3). (B) 5’NT activity of MV preparations. Values are presented as means with SD (n=5). (C) TRPV1 antagonizing effects of MV preparations in a rat dorsal root ganglion (DRG) model. Vesicles were incubated with DRGs, and calcium flux were monitored after addition of the agonist capsaicin, n=6. Boxplots with min to max and average value representation. (D) and (E) MV stimulation of IL-6 and IL-1ß secretion in PBMCs from healthy donors measured by ELISA (n=6). The significance level in all graphs is *p < 0.05.

    Techniques Used: Isolation, Cell Culture, Activity Assay, Incubation, Enzyme-linked Immunosorbent Assay


    Figure Legend Snippet: API of B. longum BG-L47, BG-L48 and BB536 under strictly anaerobic conditions. Growth was assessed by color change which was estimated manually on a scale of 0-5 * Indicates that the results varied between the five biological replicates.

    Techniques Used:


    Figure Legend Snippet: Median fecal levels of B. longum BG-L47 in participants receiving either high or low dose BG-L47 or placebo. Values are median (interquartile range) of samples shown in log 10 CFU g −1 . * Denotes significance against both other groups on day 28. ANOVA with Tukey’s adjustment and baseline correction.

    Techniques Used:

    reference strains b longum subsp longum atcc 15707  (ATCC)


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    ATCC reference strains b longum subsp longum atcc 15707
    Reference Strains B Longum Subsp Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b longum subsp longum atcc 15707  (ATCC)


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    ATCC b longum subsp longum atcc 15707
    B Longum Subsp Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b longum atcc 15707  (ATCC)


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    ATCC b longum atcc 15707
    Summary of culture-independent techniques that have been used for the determination of Bifidobacterium spp. viability in yogurt and other dairy-based products.
    B Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Bifidobacterium species viability in dairy-based probiotic foods: challenges and innovative approaches for accurate viability determination and monitoring of probiotic functionality"

    Article Title: Bifidobacterium species viability in dairy-based probiotic foods: challenges and innovative approaches for accurate viability determination and monitoring of probiotic functionality

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1327010

    Summary of culture-independent techniques that have been used for the determination of Bifidobacterium spp. viability in yogurt and other dairy-based products.
    Figure Legend Snippet: Summary of culture-independent techniques that have been used for the determination of Bifidobacterium spp. viability in yogurt and other dairy-based products.

    Techniques Used: Flow Cytometry, Probiotics, Capsules

    b longum atcc 15707  (ATCC)


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    ATCC b longum atcc 15707
    Summary of culture-independent techniques that have been used for the determination of Bifidobacterium spp. viability in yogurt and other dairy-based products.
    B Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b longum atcc 15707/product/ATCC
    Average 86 stars, based on 1 article reviews
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    b longum atcc 15707 - by Bioz Stars, 2024-05
    86/100 stars

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    1) Product Images from "Bifidobacterium species viability in dairy-based probiotic foods: challenges and innovative approaches for accurate viability determination and monitoring of probiotic functionality"

    Article Title: Bifidobacterium species viability in dairy-based probiotic foods: challenges and innovative approaches for accurate viability determination and monitoring of probiotic functionality

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1327010

    Summary of culture-independent techniques that have been used for the determination of Bifidobacterium spp. viability in yogurt and other dairy-based products.
    Figure Legend Snippet: Summary of culture-independent techniques that have been used for the determination of Bifidobacterium spp. viability in yogurt and other dairy-based products.

    Techniques Used: Flow Cytometry, Probiotics, Capsules

    bifidobacteria bifidobacterium longum atcc 15707  (ATCC)


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    ATCC bifidobacteria bifidobacterium longum atcc 15707
    Bifidobacteria Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bifidobacterium longum atcc 15707  (ATCC)


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    ATCC bifidobacterium longum atcc 15707
    Selective species-specific primers for SIHUMI member strains and L. lactis MG1363.
    Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Impact of bacteriocin-producing strains on bacterial community composition in a simplified human intestinal microbiota"

    Article Title: Impact of bacteriocin-producing strains on bacterial community composition in a simplified human intestinal microbiota

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2023.1290697


    Figure Legend Snippet: Selective species-specific primers for SIHUMI member strains and L. lactis MG1363.

    Techniques Used: Sequencing, Amplification

    Left: Cross-streaking assay to assess antagonistic interactions among SIHUMI members. A first streak of each SIHUMI strain was vertically applied across an LYHBHI agar plate. On the side, a second streak of each strain was then perpendicularly applied. Antagonism between two strains was considered positive when growth inhibition of one of them was observed in both the first and the second streaks (highlighted in dashed lines). Right: Antagonism network diagram of SIHUMI consortium in LYHBHI. Arrows represent the antagonism exerted by E. faecalis (purple), E. coli (pink), L. plantarum (green) and B. longum (blue). The origin of the arrows indicates the antagonizing strain while the arrowheads point to the antagonized strain.
    Figure Legend Snippet: Left: Cross-streaking assay to assess antagonistic interactions among SIHUMI members. A first streak of each SIHUMI strain was vertically applied across an LYHBHI agar plate. On the side, a second streak of each strain was then perpendicularly applied. Antagonism between two strains was considered positive when growth inhibition of one of them was observed in both the first and the second streaks (highlighted in dashed lines). Right: Antagonism network diagram of SIHUMI consortium in LYHBHI. Arrows represent the antagonism exerted by E. faecalis (purple), E. coli (pink), L. plantarum (green) and B. longum (blue). The origin of the arrows indicates the antagonizing strain while the arrowheads point to the antagonized strain.

    Techniques Used: Inhibition

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    ATCC b longum atcc 15707
    List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .
    B Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bifidobacterium longum subsp longum atcc 15707
    List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .
    Bifidobacterium Longum Subsp Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bifidobacterium longum atcc 15707
    List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .
    Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC control strain bifidobacterium longum atcc 15707
    MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.
    Control Strain Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC reference strains b longum subsp longum atcc 15707
    MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.
    Reference Strains B Longum Subsp Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC b longum subsp longum atcc 15707
    MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.
    B Longum Subsp Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bifidobacteria bifidobacterium longum atcc 15707
    MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.
    Bifidobacteria Bifidobacterium Longum Atcc 15707, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .

    Journal: Gut Microbes

    Article Title: Comparison of fidaxomicin, thuricin CD, vancomycin and nisin highlights the narrow spectrum nature of thuricin CD

    doi: 10.1080/19490976.2024.2342583

    Figure Lengend Snippet: List of bacterial strains used and their growth conditions. MRSA – methicillin-resistant Staphylococcus aureus , VISA – vancomycin intermediate Staphylococcus aureus .

    Article Snippet: Thuricin CD was also active against L. lactis HP (<100 µg/mL), R. gnavus JCM659ST (<25 µg/mL) and B. longum ATCC 15707 (<50 µg/mL), but only at very high concentrations.

    Techniques:

    Journal: bioRxiv

    Article Title: Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles

    doi: 10.1101/2024.02.12.579962

    Figure Lengend Snippet: MV sample names derived from L. reuteri DSM 17938 when B. longum strain was used as stimulant.

    Article Snippet: The control strain Bifidobacterium longum ATCC 15707 was used as internal control as described by the ISO method.

    Techniques: Derivative Assay

    The ability of B. longum strains to stimulate growth of L. reuteri DSM 17938 by co-cultivation in SIM was tested. 4, 10 and 25% of bifidobacterial cell suspension was added and the L. reuteri DSM 17938 cultures were grown for 48 h followed by OD 600 measurement. Significance level used was *p < 0.05.

    Journal: bioRxiv

    Article Title: Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles

    doi: 10.1101/2024.02.12.579962

    Figure Lengend Snippet: The ability of B. longum strains to stimulate growth of L. reuteri DSM 17938 by co-cultivation in SIM was tested. 4, 10 and 25% of bifidobacterial cell suspension was added and the L. reuteri DSM 17938 cultures were grown for 48 h followed by OD 600 measurement. Significance level used was *p < 0.05.

    Article Snippet: The control strain Bifidobacterium longum ATCC 15707 was used as internal control as described by the ISO method.

    Techniques: Suspension

    Physiological characterization of the B. longum strains. Scanning electron microscope images showing the morphology of BG-L47 (A) and BG-L48 (B) . The samples were sputtered with gold before imaging. Adhesion of the B. longum strains to mucus (C) . After allowing the strains to adhere to mucus-coated microtiter wells, the numbers of bacteria was determined by trypsin release and plating. Detection limit marked with a dotted red line. Bile tolerance of the B. longum strains (D) was determined by exposing the bacteria to 0.3% bovine bile for 90 minutes, after which CFU was measured. Low pH survival of the B. longum strains (E) was assessed by exposing the bacteria to synthetic gastric juice at pH 3.0. CFU was measured after 0, 20, 50 and 90 minutes of exposure. The significance level for all statistical analyzes was *p < 0.05.

    Journal: bioRxiv

    Article Title: Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles

    doi: 10.1101/2024.02.12.579962

    Figure Lengend Snippet: Physiological characterization of the B. longum strains. Scanning electron microscope images showing the morphology of BG-L47 (A) and BG-L48 (B) . The samples were sputtered with gold before imaging. Adhesion of the B. longum strains to mucus (C) . After allowing the strains to adhere to mucus-coated microtiter wells, the numbers of bacteria was determined by trypsin release and plating. Detection limit marked with a dotted red line. Bile tolerance of the B. longum strains (D) was determined by exposing the bacteria to 0.3% bovine bile for 90 minutes, after which CFU was measured. Low pH survival of the B. longum strains (E) was assessed by exposing the bacteria to synthetic gastric juice at pH 3.0. CFU was measured after 0, 20, 50 and 90 minutes of exposure. The significance level for all statistical analyzes was *p < 0.05.

    Article Snippet: The control strain Bifidobacterium longum ATCC 15707 was used as internal control as described by the ISO method.

    Techniques: Microscopy, Imaging, Bacteria

    (A) Glycoside hydrolases (GH) in B. longum BG-L47 and 4 control strains. The GHs are sorted into four categories according to the origin of the substrate: Human milk glycans, host glycans, dietary glycans and microbially derived glycans. The number of genes within a particular GH family is illustrated by a color scheme representing 0-5. (B) Fiber utilization in BG-L47 and BB536 presented together with GHs predicted to be involved in utilization of that specific fiber. Growth was determined by measuring the pH drop when cultured in mMRS with the fibers as carbon source. The explanation of the scale is 0= pH drop 0-0.1, 1= pH drop 0.2-0.5, 2= pH drop 0.5-1, 3= pH drop 1-1.5. (C) Predicted extracellular localization of BG-L47 glycoside hydrolases. The protein sequences were analyzed with SignalP, TMHMM, BLAST and subjected to manual annotation; The method for classification of cell surface localization described by Båth et al., 2005 was used.

    Journal: bioRxiv

    Article Title: Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles

    doi: 10.1101/2024.02.12.579962

    Figure Lengend Snippet: (A) Glycoside hydrolases (GH) in B. longum BG-L47 and 4 control strains. The GHs are sorted into four categories according to the origin of the substrate: Human milk glycans, host glycans, dietary glycans and microbially derived glycans. The number of genes within a particular GH family is illustrated by a color scheme representing 0-5. (B) Fiber utilization in BG-L47 and BB536 presented together with GHs predicted to be involved in utilization of that specific fiber. Growth was determined by measuring the pH drop when cultured in mMRS with the fibers as carbon source. The explanation of the scale is 0= pH drop 0-0.1, 1= pH drop 0.2-0.5, 2= pH drop 0.5-1, 3= pH drop 1-1.5. (C) Predicted extracellular localization of BG-L47 glycoside hydrolases. The protein sequences were analyzed with SignalP, TMHMM, BLAST and subjected to manual annotation; The method for classification of cell surface localization described by Båth et al., 2005 was used.

    Article Snippet: The control strain Bifidobacterium longum ATCC 15707 was used as internal control as described by the ISO method.

    Techniques: Derivative Assay, Cell Culture

    Bioactivities of MV isolated from L. reuteri DSM 17938 co-cultured with B. longum BG-L47 (MV 47 ), BB536 (MV 536 ), or grown in SIM with fructose as electron acceptor (MV u ) (A) Protein concentrations of MV preparations. Data are presented as boxplots with min to max (n=3). (B) 5’NT activity of MV preparations. Values are presented as means with SD (n=5). (C) TRPV1 antagonizing effects of MV preparations in a rat dorsal root ganglion (DRG) model. Vesicles were incubated with DRGs, and calcium flux were monitored after addition of the agonist capsaicin, n=6. Boxplots with min to max and average value representation. (D) and (E) MV stimulation of IL-6 and IL-1ß secretion in PBMCs from healthy donors measured by ELISA (n=6). The significance level in all graphs is *p < 0.05.

    Journal: bioRxiv

    Article Title: Bifidobacterium longum subsp . longum BG-L47 boosts growth and activity of Limosilactobacillus reuteri DSM 17938 and its extracellular membrane vesicles

    doi: 10.1101/2024.02.12.579962

    Figure Lengend Snippet: Bioactivities of MV isolated from L. reuteri DSM 17938 co-cultured with B. longum BG-L47 (MV 47 ), BB536 (MV 536 ), or grown in SIM with fructose as electron acceptor (MV u ) (A) Protein concentrations of MV preparations. Data are presented as boxplots with min to max (n=3). (B) 5’NT activity of MV preparations. Values are presented as means with SD (n=5). (C) TRPV1 antagonizing effects of MV preparations in a rat dorsal root ganglion (DRG) model. Vesicles were incubated with DRGs, and calcium flux were monitored after addition of the agonist capsaicin, n=6. Boxplots with min to max and average value representation. (D) and (E) MV stimulation of IL-6 and IL-1ß secretion in PBMCs from healthy donors measured by ELISA (n=6). The significance level in all graphs is *p < 0.05.

    Article Snippet: The control strain Bifidobacterium longum ATCC 15707 was used as internal control as described by the ISO method.

    Techniques: Isolation, Cell Culture, Activity Assay, Incubation, Enzyme-linked Immunosorbent Assay