Journal: Analytical methods : advancing methods and applications

Article Title: Biomonitoring method for the analysis of chromium and cobalt in human whole blood using inductively coupled plasma - kinetic energy discrimination - mass spectrometry (ICP-KED-MS)

doi: 10.1039/C7AY00430C

Figure Lengend Snippet: Instrument Parameters for the Thermo ICAP-Qc

Article Snippet: We prepared final dilutions (i.e. working calibrators, QC, and unknown samples) for analysis using a Hamilton Microlab 625 Advanced Dual Syringe Diluter (Hamilton Company, Reno, NV) equipped with a 10 mL dispensing syringe and a 500 μL sampling syringe. table ft1 table-wrap mode="anchored" t5 caption a7 Plasma Power 1550 W Cool Flow (Ar) 14 L min -1 Auxiliary Flow (Ar) 0.8 L min -1 Nebulizer Flow (Ar) ∼ 0.95 L min -1 KED Mode Gas Flow (He) 5 mL min -1 Method Parameters Sweeps/Reading 20 Survey Run 1 Replicates 3 Dwell Times 200 ms for 52 Cr and 53 Cr, 250 ms for 50 Co 100 ms for 71 Ga and 45 Sc Scan Mode Peak Hopping KED Mode Gas Flow (He) 5 mL min -1 Peristaltic Pump 40 rpm Sample Loop 2.0 mL Sample Flush Time 4 s Read Delay Time 15 s Wash Time 30 s Internal Standard 71 Ga and 45 Sc (20 μg L -1 ) Calibration Type External Simple Linear Matrix Matched Calibration (μg L -1 ) 0.0, 0.5, 1.5, 5.0, 7.5, 15.0 Sample Preparation 20× dilution Open in a separate window caption a8 Instrument Parameters for the Thermo ICAP-Qc Statistical Analyses A mixed effect of the analysis of variance (ANOVA) was used to examine ruggedness.

Techniques: Sample Prep