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    Structured Review

    ATCC biofilms
    Quantification of fluorescence intensity during biocide treatments. The values shown represent the loss of fluorescence at three different areas: 1 (■), 2 (▩), and 3 (□) in the <t>biofilm</t> cluster under treatment with 0.05% PAA (A)
    Biofilms, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Dynamics of the Action of Biocides in Pseudomonas aeruginosa Biofilms ▿ Biofilms ▿ †"

    Article Title: Dynamics of the Action of Biocides in Pseudomonas aeruginosa Biofilms ▿ Biofilms ▿ †

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01760-10

    Quantification of fluorescence intensity during biocide treatments. The values shown represent the loss of fluorescence at three different areas: 1 (■), 2 (▩), and 3 (□) in the biofilm cluster under treatment with 0.05% PAA (A)
    Figure Legend Snippet: Quantification of fluorescence intensity during biocide treatments. The values shown represent the loss of fluorescence at three different areas: 1 (■), 2 (▩), and 3 (□) in the biofilm cluster under treatment with 0.05% PAA (A)

    Techniques Used: Fluorescence

    Resistance of biofilms and planktonic cells to biocides.
    Figure Legend Snippet: Resistance of biofilms and planktonic cells to biocides.

    Techniques Used:

    Visualization of Chemchrome V6 fluorescence loss (cell membrane permeabilization) in P. aeruginosa ATCC 15442 biofilms during treatments with PAA and BAC biocides after 0, 5, 10, 15, 20, and 25 min of application. Each image corresponds to the superimposition
    Figure Legend Snippet: Visualization of Chemchrome V6 fluorescence loss (cell membrane permeabilization) in P. aeruginosa ATCC 15442 biofilms during treatments with PAA and BAC biocides after 0, 5, 10, 15, 20, and 25 min of application. Each image corresponds to the superimposition

    Techniques Used: Fluorescence, BAC Assay

    Visualization and modeling of biocide action in P. aeruginosa biofilms.
    Figure Legend Snippet: Visualization and modeling of biocide action in P. aeruginosa biofilms.

    Techniques Used:

    Visualization of Chemchrome V6 fluorescence loss (cell membrane permeabilization) in P. aeruginosa clinical isolate biofilms during BAC treatments after 0, 5, 10, 15, 20, and 25 min of application. Each image corresponds to the superimposition of green
    Figure Legend Snippet: Visualization of Chemchrome V6 fluorescence loss (cell membrane permeabilization) in P. aeruginosa clinical isolate biofilms during BAC treatments after 0, 5, 10, 15, 20, and 25 min of application. Each image corresponds to the superimposition of green

    Techniques Used: Fluorescence, BAC Assay

    Sugar (black bars) and protein (gray bars) levels in the biofilm of the four P. aeruginosa strains. Values (μg/well) correspond to the mean of three independent experiments and are shown inside the bars. Error bars represent the standard deviations.
    Figure Legend Snippet: Sugar (black bars) and protein (gray bars) levels in the biofilm of the four P. aeruginosa strains. Values (μg/well) correspond to the mean of three independent experiments and are shown inside the bars. Error bars represent the standard deviations.

    Techniques Used:

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    ATCC pseudomonas aeruginosa
    The number of colony-forming units (CFU) in different bacterial strains ( S. aureus (ATCC 6538), P. <t>aeruginosa</t> (ATCC 15442), S. enterica (ATCC 10708), and E. coli (ATCC 25922)) was counted. CFU counting was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 1, 10, 20, 30, and 40 min.
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    The number of colony-forming units (CFU) in different bacterial strains ( S. aureus (ATCC 6538), P. aeruginosa (ATCC 15442), S. enterica (ATCC 10708), and E. coli (ATCC 25922)) was counted. CFU counting was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 1, 10, 20, 30, and 40 min.

    Journal: Molecules

    Article Title: Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria

    doi: 10.3390/molecules27133998

    Figure Lengend Snippet: The number of colony-forming units (CFU) in different bacterial strains ( S. aureus (ATCC 6538), P. aeruginosa (ATCC 15442), S. enterica (ATCC 10708), and E. coli (ATCC 25922)) was counted. CFU counting was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 1, 10, 20, 30, and 40 min.

    Article Snippet: Standard strains (Staphylococcus aureus (ATCC 6538), Salmonella enterica subsp. enterica serovar choleraesuis (ATCC 10708), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 15442)) were obtained from the American Type Culture Collection (ATCC) (Plast Labor Ind.

    Techniques:

    Analysis of cell viability after ozone treatment in different bacterial strains ( S. aureus (ATCC 6538), S. enterica (ATCC 10708), E. coli (ATCC 25922), and P. aeruginosa (ATCC 15442)). The measurement of fluorescence intensity (relative fluorescence units, RFU) after the conversion of resazurin to resofurin by viable bacteria was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 40 min. Results represent values from 3 randomly chosen colonies in the control group (no treatment) and after treatment with ozone. The black dots represent the values of fluorescence emission after addition of resazurin.

    Journal: Molecules

    Article Title: Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria

    doi: 10.3390/molecules27133998

    Figure Lengend Snippet: Analysis of cell viability after ozone treatment in different bacterial strains ( S. aureus (ATCC 6538), S. enterica (ATCC 10708), E. coli (ATCC 25922), and P. aeruginosa (ATCC 15442)). The measurement of fluorescence intensity (relative fluorescence units, RFU) after the conversion of resazurin to resofurin by viable bacteria was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 40 min. Results represent values from 3 randomly chosen colonies in the control group (no treatment) and after treatment with ozone. The black dots represent the values of fluorescence emission after addition of resazurin.

    Article Snippet: Standard strains (Staphylococcus aureus (ATCC 6538), Salmonella enterica subsp. enterica serovar choleraesuis (ATCC 10708), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 15442)) were obtained from the American Type Culture Collection (ATCC) (Plast Labor Ind.

    Techniques: Fluorescence

    ( A ) The number of colony-forming units (CFU) in different bacterial strains ( S. aureus (MRSA), P. aeruginosa (XDR), A. baumannii (PDR), and K. pneumoniae (KPC+)) were counted. The number of CFU in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 40 min was quantified. The black dots represent the number of CFU count of the different strains. ( B ) Analysis of cell viability after ozone treatment in different bacterial strains ( S. aureus (MRSA), P. aeruginosa (XDR), A. baumannii (PDR), and K. pneumoniae (KPC+)). The measurement of fluorescence intensity (Relative fluorescence units, RFU) after the conversion of resazurin to resofurin by viable bacteria was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 40 min. Results represent three randomly chosen colonies in the control group (no treatment) and after ozone treatment. The black dots show cell viability values through fluorescence emission after the addition of resazurin. ** Statistically significant ( p

    Journal: Molecules

    Article Title: Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria

    doi: 10.3390/molecules27133998

    Figure Lengend Snippet: ( A ) The number of colony-forming units (CFU) in different bacterial strains ( S. aureus (MRSA), P. aeruginosa (XDR), A. baumannii (PDR), and K. pneumoniae (KPC+)) were counted. The number of CFU in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 40 min was quantified. The black dots represent the number of CFU count of the different strains. ( B ) Analysis of cell viability after ozone treatment in different bacterial strains ( S. aureus (MRSA), P. aeruginosa (XDR), A. baumannii (PDR), and K. pneumoniae (KPC+)). The measurement of fluorescence intensity (Relative fluorescence units, RFU) after the conversion of resazurin to resofurin by viable bacteria was performed in the control group (no treatment) and bacterial suspensions (10 5 CFU/mL) after exposure to ozone for 40 min. Results represent three randomly chosen colonies in the control group (no treatment) and after ozone treatment. The black dots show cell viability values through fluorescence emission after the addition of resazurin. ** Statistically significant ( p

    Article Snippet: Standard strains (Staphylococcus aureus (ATCC 6538), Salmonella enterica subsp. enterica serovar choleraesuis (ATCC 10708), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 15442)) were obtained from the American Type Culture Collection (ATCC) (Plast Labor Ind.

    Techniques: Fluorescence

    Morphological analysis of O 3 treatment by electron microscopy. S. aureus (MRSA) ( a , b ) and P. aeruginosa (XDR) ( c , d ) are seen without ( a , c ) and under O 3 treatment ( b , d ). An alteration in S. aureus (MRSA) shape is seen (arrowhead) in b. Damage in bacteria is observed after treatment (arrows) ( b ). Note that control cells are rounded and present in a homogeneous surface ( a ). Damaged cells are observed after treatment in P. aeruginosa (XDR) (arrows) ( d ). Some cell wall protrusions are observed in treated cells (arrowhead) ( d ). These aspects were not verified in control cells ( c ).

    Journal: Molecules

    Article Title: Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria

    doi: 10.3390/molecules27133998

    Figure Lengend Snippet: Morphological analysis of O 3 treatment by electron microscopy. S. aureus (MRSA) ( a , b ) and P. aeruginosa (XDR) ( c , d ) are seen without ( a , c ) and under O 3 treatment ( b , d ). An alteration in S. aureus (MRSA) shape is seen (arrowhead) in b. Damage in bacteria is observed after treatment (arrows) ( b ). Note that control cells are rounded and present in a homogeneous surface ( a ). Damaged cells are observed after treatment in P. aeruginosa (XDR) (arrows) ( d ). Some cell wall protrusions are observed in treated cells (arrowhead) ( d ). These aspects were not verified in control cells ( c ).

    Article Snippet: Standard strains (Staphylococcus aureus (ATCC 6538), Salmonella enterica subsp. enterica serovar choleraesuis (ATCC 10708), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 15442)) were obtained from the American Type Culture Collection (ATCC) (Plast Labor Ind.

    Techniques: Electron Microscopy

    Average temperature, relative humidity, and ozone concentration at times of 1, 10, 20, 30, and 40 min with ATCC ( S. aureus (ATCC 6538), P. aeruginosa (ATCC 15442), S. enterica (ATCC) strains 10708), and E. coli (ATCC 25922)) and multidrug-resistant S. aureus (MRSA), P. aeruginosa (XDR), A. baumannii (PDR), and K. pneumoniae (KPC+).

    Journal: Molecules

    Article Title: Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria

    doi: 10.3390/molecules27133998

    Figure Lengend Snippet: Average temperature, relative humidity, and ozone concentration at times of 1, 10, 20, 30, and 40 min with ATCC ( S. aureus (ATCC 6538), P. aeruginosa (ATCC 15442), S. enterica (ATCC) strains 10708), and E. coli (ATCC 25922)) and multidrug-resistant S. aureus (MRSA), P. aeruginosa (XDR), A. baumannii (PDR), and K. pneumoniae (KPC+).

    Article Snippet: Standard strains (Staphylococcus aureus (ATCC 6538), Salmonella enterica subsp. enterica serovar choleraesuis (ATCC 10708), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 15442)) were obtained from the American Type Culture Collection (ATCC) (Plast Labor Ind.

    Techniques: Concentration Assay

    Combinations of Cu 2+ with other QACs show synergistic killing of P. aeruginosa ATCC 15442 biofilms. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and benzalkonium chloride (a), cetylpyridinium chloride

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Copper and Quaternary Ammonium Cations Exert Synergistic Bactericidal and Antibiofilm Activity against Pseudomonas aeruginosa

    doi: 10.1128/AAC.00203-08

    Figure Lengend Snippet: Combinations of Cu 2+ with other QACs show synergistic killing of P. aeruginosa ATCC 15442 biofilms. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and benzalkonium chloride (a), cetylpyridinium chloride

    Article Snippet: Although the explicit focus of this report is biofilm susceptibility testing, combinations of Cu2+ and QACs also killed planktonic cells of P. aeruginosa ATCC 15442 at concentrations that were at least 10-fold less than the concentrations required to treat biofilms (J. J. Harrison, D. J. Joo, R. J. Turner, and H. Ceri, unpublished data).

    Techniques:

    Cu 2+ and Polycide, alone and in combination, negatively affect cell survival and nitrate (NO 3 ) reduction in microaerobic P. aeruginosa ATCC 15442 cultures. Mean viable cell counts (a) and log killing of planktonic cell populations (b) were determined

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Copper and Quaternary Ammonium Cations Exert Synergistic Bactericidal and Antibiofilm Activity against Pseudomonas aeruginosa

    doi: 10.1128/AAC.00203-08

    Figure Lengend Snippet: Cu 2+ and Polycide, alone and in combination, negatively affect cell survival and nitrate (NO 3 ) reduction in microaerobic P. aeruginosa ATCC 15442 cultures. Mean viable cell counts (a) and log killing of planktonic cell populations (b) were determined

    Article Snippet: Although the explicit focus of this report is biofilm susceptibility testing, combinations of Cu2+ and QACs also killed planktonic cells of P. aeruginosa ATCC 15442 at concentrations that were at least 10-fold less than the concentrations required to treat biofilms (J. J. Harrison, D. J. Joo, R. J. Turner, and H. Ceri, unpublished data).

    Techniques:

    P. aeruginosa ATCC 15442 biofilms were killed time dependently by combinations of Cu 2+ and Polycide. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and Polycide in ddH 2 O for 10 min (a) or 30 min (b)

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Copper and Quaternary Ammonium Cations Exert Synergistic Bactericidal and Antibiofilm Activity against Pseudomonas aeruginosa

    doi: 10.1128/AAC.00203-08

    Figure Lengend Snippet: P. aeruginosa ATCC 15442 biofilms were killed time dependently by combinations of Cu 2+ and Polycide. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and Polycide in ddH 2 O for 10 min (a) or 30 min (b)

    Article Snippet: Although the explicit focus of this report is biofilm susceptibility testing, combinations of Cu2+ and QACs also killed planktonic cells of P. aeruginosa ATCC 15442 at concentrations that were at least 10-fold less than the concentrations required to treat biofilms (J. J. Harrison, D. J. Joo, R. J. Turner, and H. Ceri, unpublished data).

    Techniques:

    Effect of rhamnolipids and sophorolipids on Biofilm disruption. Biofilms were grown for 48 h at 30 °C and then stained with Live/Dead BacLight and the images were recorded with a microscope Evon (×10) (17 % light). a P. aeruginosa ATCC 15442 biofilm before treatment. b P. aeruginosa ATCC 15442 after treatment with rhamnolipid (0.04 %) and sophorolipid (0.01 %). c S. aureus ATCC 9144 before treatment. d S. aureus ATCC 9144 after treatment with rhamnolipid (0.04 %) and sophorolipid (0.01 %). e Mixed culture ( P. aeruginosa ATCC 15442/ S. aureus ATCC 9144). f Mixed culture after treatment with rhamnolipid (0.04 %) and sophorolipid (0.01 %)

    Journal: Applied Microbiology and Biotechnology

    Article Title: Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel

    doi: 10.1007/s00253-016-7310-5

    Figure Lengend Snippet: Effect of rhamnolipids and sophorolipids on Biofilm disruption. Biofilms were grown for 48 h at 30 °C and then stained with Live/Dead BacLight and the images were recorded with a microscope Evon (×10) (17 % light). a P. aeruginosa ATCC 15442 biofilm before treatment. b P. aeruginosa ATCC 15442 after treatment with rhamnolipid (0.04 %) and sophorolipid (0.01 %). c S. aureus ATCC 9144 before treatment. d S. aureus ATCC 9144 after treatment with rhamnolipid (0.04 %) and sophorolipid (0.01 %). e Mixed culture ( P. aeruginosa ATCC 15442/ S. aureus ATCC 9144). f Mixed culture after treatment with rhamnolipid (0.04 %) and sophorolipid (0.01 %)

    Article Snippet: In this study, we have confirmed the biofilm disruption of P. aeruginosa ATCC 15442, S. aureus ATCC 9144 and a mixed culture using rhamnolipids and caprylic acid, as well as the greater sensitivity of S. aureus ATCC 9144 over P. aeruginosa in terms of disruption and viability as shown by Live/Dead staining.

    Techniques: Staining, Microscopy

    Oxygen consumption of P. aeruginosa ATCC 15442 biofilms after 30-min treatment with a combinations of biosurfactants and b mono- and di-rhamnolipids. Shown in a plot of the relative concentration of dissolved oxygen in percentage of saturation concentration versus time after addition of the different substances. Treatment concentrations are indicated

    Journal: Applied Microbiology and Biotechnology

    Article Title: Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel

    doi: 10.1007/s00253-016-7310-5

    Figure Lengend Snippet: Oxygen consumption of P. aeruginosa ATCC 15442 biofilms after 30-min treatment with a combinations of biosurfactants and b mono- and di-rhamnolipids. Shown in a plot of the relative concentration of dissolved oxygen in percentage of saturation concentration versus time after addition of the different substances. Treatment concentrations are indicated

    Article Snippet: In this study, we have confirmed the biofilm disruption of P. aeruginosa ATCC 15442, S. aureus ATCC 9144 and a mixed culture using rhamnolipids and caprylic acid, as well as the greater sensitivity of S. aureus ATCC 9144 over P. aeruginosa in terms of disruption and viability as shown by Live/Dead staining.

    Techniques: Concentration Assay

    Biofilm formation and disruption in a BioFlux channel. The images are phase-contrast images and show fully formed biofilms after 48 h of incubation at 30 °C, and the images were recorded with a microscope Evon (10×) (17 % light). a P. aeruginosa ATCC 15442 biofilm before treatment. b P. aeruginosa ATCC 15442 after treatment with rhamnolipid (0.04 %) and caprylic acid (0.01 %). c S. aureus ATCC 9144 before treatment. d S. aureus ATCC 9144 after treatment with rhamnolipid (0.04 %) and caprylic acid (0.01 %). e Mixed culture ( P. aeruginosa ATCC 15442/ S. aureus ATCC 9144). f Mixed culture after treatment with rhamnolipid (0.04 %) and caprylic acid (0.01 %). g P. aeruginosa ATCC 15442 before treatment. h P. aeruginosa ATCC 15442 after treatment with PBS 1×

    Journal: Applied Microbiology and Biotechnology

    Article Title: Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel

    doi: 10.1007/s00253-016-7310-5

    Figure Lengend Snippet: Biofilm formation and disruption in a BioFlux channel. The images are phase-contrast images and show fully formed biofilms after 48 h of incubation at 30 °C, and the images were recorded with a microscope Evon (10×) (17 % light). a P. aeruginosa ATCC 15442 biofilm before treatment. b P. aeruginosa ATCC 15442 after treatment with rhamnolipid (0.04 %) and caprylic acid (0.01 %). c S. aureus ATCC 9144 before treatment. d S. aureus ATCC 9144 after treatment with rhamnolipid (0.04 %) and caprylic acid (0.01 %). e Mixed culture ( P. aeruginosa ATCC 15442/ S. aureus ATCC 9144). f Mixed culture after treatment with rhamnolipid (0.04 %) and caprylic acid (0.01 %). g P. aeruginosa ATCC 15442 before treatment. h P. aeruginosa ATCC 15442 after treatment with PBS 1×

    Article Snippet: In this study, we have confirmed the biofilm disruption of P. aeruginosa ATCC 15442, S. aureus ATCC 9144 and a mixed culture using rhamnolipids and caprylic acid, as well as the greater sensitivity of S. aureus ATCC 9144 over P. aeruginosa in terms of disruption and viability as shown by Live/Dead staining.

    Techniques: Incubation, Microscopy