150 kd protein concentrator  (Thermo Fisher)


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    Thermo Fisher 150 kd protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    150 Kd Protein Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150 kd protein concentrator/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    150 kd protein concentrator - by Bioz Stars, 2020-04
    95/100 stars

    Images

    1) Product Images from "The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma"

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma

    Journal: Cancers

    doi: 10.3390/cancers11121823

    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    Figure Legend Snippet: SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Techniques Used: Expressing, Quantitative RT-PCR, Activity Assay, Isolation

    Related Articles

    Concentration Assay:

    Article Title: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts
    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter. .. The concentration of EV proteins was determined by BCA protein analysis (Pierce BCA protein Assay, Thermo Scientific).

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma
    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter. .. The concentration of EV proteins was determined by BCA protein analysis (Thermo Scientific).

    Isolation:

    Article Title: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts
    Article Snippet: Paragraph title: Isolation and characterization of small EVs ... The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma
    Article Snippet: Paragraph title: 4.6. Isolation and Characterization of Small EVs ... The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Centrifugation:

    Article Title: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts
    Article Snippet: Isolation and characterization of small EVs 5TGM1 cells were cultured without serum for 24 h and conditioned medium (CM) was collected after centrifugation and filtered using a 0.22 µM pore filter (Carl Roth, Karlsruhe, Germany). .. The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma
    Article Snippet: Isolation and Characterization of Small EVs JJN3, LP1, OPM2, and U266 cells were cultured without serum for 24 h and conditioned medium was collected after centrifugation and filtered using a 0.22 µM pore filter. .. The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Cell Culture:

    Article Title: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts
    Article Snippet: Isolation and characterization of small EVs 5TGM1 cells were cultured without serum for 24 h and conditioned medium (CM) was collected after centrifugation and filtered using a 0.22 µM pore filter (Carl Roth, Karlsruhe, Germany). .. The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma
    Article Snippet: Isolation and Characterization of Small EVs JJN3, LP1, OPM2, and U266 cells were cultured without serum for 24 h and conditioned medium was collected after centrifugation and filtered using a 0.22 µM pore filter. .. The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    BIA-KA:

    Article Title: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts
    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter. .. The concentration of EV proteins was determined by BCA protein analysis (Pierce BCA protein Assay, Thermo Scientific).

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma
    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter. .. The concentration of EV proteins was determined by BCA protein analysis (Thermo Scientific).

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    Thermo Fisher 150 kd protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    150 Kd Protein Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150 kd protein concentrator/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    150 kd protein concentrator - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Journal: Cancers

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma

    doi: 10.3390/cancers11121823

    Figure Lengend Snippet: SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Isolation