Structured Review

Illumina Inc 150 bp single end sequencing
150 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/150 bp single end sequencing/product/Illumina Inc
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
150 bp single end sequencing - by Bioz Stars, 2020-05
93/100 stars

Images

Related Articles

Sequencing:

Article Title: A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection
Article Snippet: .. Pooled, multiplexed libraries were subjected to 150-bp single-end sequencing on an Illumina NextSeq 500. .. The resulting data were demultiplexed using bcl2fastq2 (Illumina) to obtain fastq files for downstream analysis.

Article Title: A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection
Article Snippet: .. The libraries were multiplexed and subjected to 150-bp single-end sequencing on an Illumina NextSeq 500. .. The resulting data were demultiplexed using bcl2fastq2 (Illumina) to obtain fastq files for downstream analysis.

Article Title: Divergent Evolutionary Trajectories of Two Young, Homomorphic, and Closely Related Sex Chromosome Systems
Article Snippet: .. The RADSeq library was generated by Floragenex (Portland, OR) on both X. laevis parents, 17 daughters, and 20 sons and 150-bp single-end sequencing was performed at the University of Oregon using an Illumina HiSeq 2500 machine. .. Though slightly different procedures were used to generate reduced representation genome sequences from each species, the nature of the data is essentially the same—both methods produced sequence data from many homologous regions in most or all individuals from each family.

Article Title: Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
Article Snippet: .. The second ten-cycle PCR step, the purification (Ampure Bead clean up), the pooling, the gel excised purification, and the 150-bp single-end sequencing on an Illumina MiSeq instrument were performed by Microsynth AG. .. Western blotting N2a.EGFP cells were cultured on a 48-well plate and transfected as described above in the “EGFP disruption assay” section.

Article Title: Divergent Evolutionary Trajectories of Two Young, Homomorphic, and Closely Related Sex Chromosome Systems
Article Snippet: .. The RADSeq library was generated by Floragenex (Portland, OR) on both X. laevis parents, 17 daughters, and 20 sons and 150-bp single-end sequencing was performed at the University of Oregon using an Illumina HiSeq 2500 machine. .. Though slightly different procedures were used to generate reduced representation genome sequences from each species, the nature of the data is essentially the same—both methods produced sequence data from many homologous regions in most or all individuals from each family.

Polymerase Chain Reaction:

Article Title: Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
Article Snippet: .. The second ten-cycle PCR step, the purification (Ampure Bead clean up), the pooling, the gel excised purification, and the 150-bp single-end sequencing on an Illumina MiSeq instrument were performed by Microsynth AG. .. Western blotting N2a.EGFP cells were cultured on a 48-well plate and transfected as described above in the “EGFP disruption assay” section.

Generated:

Article Title: Divergent Evolutionary Trajectories of Two Young, Homomorphic, and Closely Related Sex Chromosome Systems
Article Snippet: .. The RADSeq library was generated by Floragenex (Portland, OR) on both X. laevis parents, 17 daughters, and 20 sons and 150-bp single-end sequencing was performed at the University of Oregon using an Illumina HiSeq 2500 machine. .. Though slightly different procedures were used to generate reduced representation genome sequences from each species, the nature of the data is essentially the same—both methods produced sequence data from many homologous regions in most or all individuals from each family.

Article Title: Divergent Evolutionary Trajectories of Two Young, Homomorphic, and Closely Related Sex Chromosome Systems
Article Snippet: .. The RADSeq library was generated by Floragenex (Portland, OR) on both X. laevis parents, 17 daughters, and 20 sons and 150-bp single-end sequencing was performed at the University of Oregon using an Illumina HiSeq 2500 machine. .. Though slightly different procedures were used to generate reduced representation genome sequences from each species, the nature of the data is essentially the same—both methods produced sequence data from many homologous regions in most or all individuals from each family.

Purification:

Article Title: Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
Article Snippet: .. The second ten-cycle PCR step, the purification (Ampure Bead clean up), the pooling, the gel excised purification, and the 150-bp single-end sequencing on an Illumina MiSeq instrument were performed by Microsynth AG. .. Western blotting N2a.EGFP cells were cultured on a 48-well plate and transfected as described above in the “EGFP disruption assay” section.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Illumina Inc 150 bp paired end
    Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, <t>150</t> μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h Validation of excitatory neurons expressing myocardial protein TNNT2 in the 23WF ST region by immunofluorescence. The PAO region is displayed as a negative control. TNNT2 antibody was tested in the cadiomyocytes as shown in Supplementary information, Figure S 6c . Scale bar, 50 μm
    150 Bp Paired End, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150 bp paired end/product/Illumina Inc
    Average 97 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    150 bp paired end - by Bioz Stars, 2020-05
    97/100 stars
      Buy from Supplier

    93
    Illumina Inc illumina hiseq2500
    Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, <t>150</t> μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h Validation of excitatory neurons expressing myocardial protein TNNT2 in the 23WF ST region by immunofluorescence. The PAO region is displayed as a negative control. TNNT2 antibody was tested in the cadiomyocytes as shown in Supplementary information, Figure S 6c . Scale bar, 50 μm
    Illumina Hiseq2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq2500/product/Illumina Inc
    Average 93 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq2500 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    99
    Illumina Inc illumina rna sequencing
    Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, <t>150</t> μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h Validation of excitatory neurons expressing myocardial protein TNNT2 in the 23WF ST region by immunofluorescence. The PAO region is displayed as a negative control. TNNT2 antibody was tested in the cadiomyocytes as shown in Supplementary information, Figure S 6c . Scale bar, 50 μm
    Illumina Rna Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina rna sequencing/product/Illumina Inc
    Average 99 stars, based on 339 article reviews
    Price from $9.99 to $1999.99
    illumina rna sequencing - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, 150 μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h Validation of excitatory neurons expressing myocardial protein TNNT2 in the 23WF ST region by immunofluorescence. The PAO region is displayed as a negative control. TNNT2 antibody was tested in the cadiomyocytes as shown in Supplementary information, Figure S 6c . Scale bar, 50 μm

    Journal: Cell Research

    Article Title: Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis

    doi: 10.1038/s41422-018-0053-3

    Figure Lengend Snippet: Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, 150 μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h Validation of excitatory neurons expressing myocardial protein TNNT2 in the 23WF ST region by immunofluorescence. The PAO region is displayed as a negative control. TNNT2 antibody was tested in the cadiomyocytes as shown in Supplementary information, Figure S 6c . Scale bar, 50 μm

    Article Snippet: Each single cell was sequenced for 2 × 106 of 150-bp paired-end reads using an Illumina HiSeq 4000.

    Techniques: Immunofluorescence, In Situ Hybridization, Quantitative RT-PCR, Expressing, Negative Control

    Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, 150 μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h . Scale bar, 50 μm

    Journal: Cell Research

    Article Title: Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis

    doi: 10.1038/s41422-018-0053-3

    Figure Lengend Snippet: Spatial differences in the developing human cortex. a Pie chart displaying the cell type constitution in the four cerebral lobes and in the inferior region of cerebral cortex. b Immunofluorescence of GFAP in the pons, PC and IT regions of a 23 W female sample. The statistics of GFAP + cell ratio in each region are shown in the histogram. Scale bar, 50 μm. c In situ hybridization of astrocyte genes RAMP3 (Astro_1) and PTGDS (Astro_2) showing higher abundance of the two subtypes of astrocytes in pons than that in IT and PC regions. Moreover, the PC shows the lowest astrocyte density. Scale bar, 150 μm. d–f DEGs across all cerebral cortex regions that are detected with more than 5 inhibitory neurons ( d ), immature excitatory neurons (Ex_1/2, e ) and mature excitatory neurons (Ex_3/4, f ). GAD1 and NEUROD2 are the housekeeping control for inhibitory and excitatory neurons, respectively. g RT-qPCR of NRGN in IG, IT, PC, and SP regions. The NRGN abundance in each region was normalized by GAPDH . h . Scale bar, 50 μm

    Article Snippet: Each single cell was sequenced for 2 × 106 of 150-bp paired-end reads using an Illumina HiSeq 4000.

    Techniques: Immunofluorescence, In Situ Hybridization, Quantitative RT-PCR