Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: ( a ) Schematic of 10 μm wide gold electrodes with interdigitated with 10 μm glass substrate immobilized with melittin. The optical image shows bacteria captured on the glass substrate. ( b ) A vertical-capacitance sensor attached vertically to a 2 mL bottle. The inset shows a vertical-capacitance sensor.

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Bacteria

Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: ( a ) Frequency−dependent capacitance measurements of the vertical−capacitance sensor in bacteria−free medium. Hollow black squares represent the values before melittin immobilization and solid black squares represent those after immobilization. The color symbols indicate the data collected from the melittin-immobilized vertical−capacitance sensor and 1 × 10 5 CFU/mL bacterial solutions. Black symbols indicate the data collected from bacteria−free medium in the sensor immobilized with melittin. Red circles, E. coli ; blue upward−pointing triangle: P. aeruginosa ; Magenta downward−pointing triangle: S. aureus ; Green diamond: E. faecalis . ( b ) Real−time capacitance measured for the melittin-immobilized capacitance array cultured with 1 × 10 5 CFU/mL of different bacterial species. ( c ) Real−time capacitance measured for the melittin−immobilized capacitance array during the cultivation of E. coli at varying concentrations. ( d ) Real-time capacitance measured for the melittin−immobilized capacitance array during the cultivation of S. aureus at varying concentrations. The data are presented as the mean ± standard deviation, with a sample size of n ≥ 3.

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Bacteria, Cell Culture, Standard Deviation

Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: Real-time capacitance changes observed in a melittin-immobilized vertical-capacitance sensor treated with 1 × 10 3 CFU/mL with standard bacteria (dot line) and 1 × 10 5 CFU/mL of clinically isolated bacteria (solid line) of ( a ) E. coli , ( b ) P. aeruginosa , ( c ) S. aureus , and ( d ) E. faecalis in media at f = 0.5 kHz. The data are presented as the mean ± standard deviation, with a sample size of n ≥ 3.

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Bacteria, Isolation, Standard Deviation

Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: ( a ) Real-time capacitance changes measured for the aptamer or melittin-immobilized vertical-capacitance sensor treated with 1 × 10 3 CFU/mL standard E. coli . The data are presented as the mean ± standard deviation, with a sample size of n ≥ 3. ( b ) Images of biofilm formation on aptamer- or melittin-immobilized sensors stained with crystal violet after 18 h. ( c ) Schematic of biofilm formation on aptamer- or melittin-immobilized sensors.

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Standard Deviation, Staining

Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: Frequency−dependence behavior of ( a ) capacitance, ( b ) conductance, and ( c ) impedance in bacteria−free medium and blood culture media of different blood:broth ratios (1:50 and 1:100) before and after immobilizing melittin on the vertical−capacitance sensor surface.

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Bacteria

Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: Frequency-dependence behavior of the capacitance in a melittin-immobilized vertical-capacitance sensor with ( a ) standard and ( b – d ) clinically isolated S. aureus strain (1 × 10 3 CFU/mL) in culture media of different blood:broth ratios (1:50 and 1:100).

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Isolation

Journal: Sensors (Basel, Switzerland)

Article Title: Pathogenic Bacterial Detection Using Vertical-Capacitance Sensor Array Immobilized with the Antimicrobial Peptide Melittin

doi: 10.3390/s25010012

Figure Lengend Snippet: Real-time capacitance measured in a melittin-immobilized vertical-capacitance sensor with 1 × 10 3 CFU/mL of E. coli or S. aureus in varying ratios of blood culture media ( a , c ) 1:50 and ( b , d ) 1:100. The real-time capacitance was measured at f = 0.5 kHz. The data are presented as the mean ± standard deviation, with a sample size of n ≥ 3.

Article Snippet: We prepared an aptamer-immobilized vertical-capacitance sensor using previously reported methods [ , ] and measured real-time capacitance changes in both aptamer- and melittin-immobilized capacitance sensors using a 10 3 CFU/mL E. coli ATCC 25922 solution in blood culture media at a frequency of 0.5 kHz.

Techniques: Standard Deviation

Journal: Pharmaceuticals

Article Title: Antimicrobial and Oxidative Activities of Different Levels of Silver-Exchanged Zeolites X and ZSM-5 and Their Ecotoxicity

doi: 10.3390/ph17121586

Figure Lengend Snippet: Materials’ description, including sample batch notation, the parent zeolite Si-to-Al ratio, silver content, and the observed silver forms: isolated ionic species and metallic silver nanoparticles. The ivory color indicated for the zeolite–X materials comes from the binder material and is not specific to silver-exchanged X-zeolites, which are white.

Article Snippet: SA12-Ag exhibited only a 10% inhibitory effect against E. coli ATCC 25922 , at the same concentration.

Techniques: Isolation

Journal: Pharmaceuticals

Article Title: Antimicrobial and Oxidative Activities of Different Levels of Silver-Exchanged Zeolites X and ZSM-5 and Their Ecotoxicity

doi: 10.3390/ph17121586

Figure Lengend Snippet: TEM images of SA12-Ag, 3.5%wt silver loading material, in which some scarce nanocrystalline silver was found too.

Article Snippet: SA12-Ag exhibited only a 10% inhibitory effect against E. coli ATCC 25922 , at the same concentration.

Techniques:

Journal: Pharmaceuticals

Article Title: Antimicrobial and Oxidative Activities of Different Levels of Silver-Exchanged Zeolites X and ZSM-5 and Their Ecotoxicity

doi: 10.3390/ph17121586

Figure Lengend Snippet: Results for E. coli ATCC 25922 at the 4th h of impact with other zeolite materials, calculated in CFU/mL.

Article Snippet: SA12-Ag exhibited only a 10% inhibitory effect against E. coli ATCC 25922 , at the same concentration.

Techniques: Concentration Assay, Control

Journal: Pharmaceuticals

Article Title: Antimicrobial and Oxidative Activities of Different Levels of Silver-Exchanged Zeolites X and ZSM-5 and Their Ecotoxicity

doi: 10.3390/ph17121586

Figure Lengend Snippet: Results for E. coli ATCC 25922 at the 24th h of impact with other zeolite materials, calculated in CFU/mL.

Article Snippet: SA12-Ag exhibited only a 10% inhibitory effect against E. coli ATCC 25922 , at the same concentration.

Techniques: Concentration Assay, Control

Journal: Pharmaceuticals

Article Title: Antimicrobial and Oxidative Activities of Different Levels of Silver-Exchanged Zeolites X and ZSM-5 and Their Ecotoxicity

doi: 10.3390/ph17121586

Figure Lengend Snippet: Results for S. aureus 25923, at the 4th h of impact, calculated in CFU/mL.

Article Snippet: SA12-Ag exhibited only a 10% inhibitory effect against E. coli ATCC 25922 , at the same concentration.

Techniques: Concentration Assay, Control