s marcescens ci  (ATCC)


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    Structured Review

    ATCC s marcescens ci
    Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. <t>marcescens</t> CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d e ) and intestinal colonization ( d , e f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p
    S Marcescens Ci, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s marcescens ci/product/ATCC
    Average 88 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    s marcescens ci - by Bioz Stars, 2022-09
    88/100 stars

    Images

    1) Product Images from "Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins"

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16507-x

    Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d e ) and intestinal colonization ( d , e f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p
    Figure Legend Snippet: Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d e ) and intestinal colonization ( d , e f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p

    Techniques Used: Infection, Inhibition, Colony-forming Unit Assay

    Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p
    Figure Legend Snippet: Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p

    Techniques Used: Confocal Laser Scanning Microscopy, Activity Assay

    MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p
    Figure Legend Snippet: MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p

    Techniques Used: Concentration Assay, Cell Surface Hydrophobicity

    Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.
    Figure Legend Snippet: Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.

    Techniques Used:

    Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.
    Figure Legend Snippet: Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.

    Techniques Used: Nucleic Acid Electrophoresis, Molecular Weight, SDS Page, Staining, Mass Spectrometry

    Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p
    Figure Legend Snippet: Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p

    Techniques Used: Concentration Assay, Inhibition, Incubation

    Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.
    Figure Legend Snippet: Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.

    Techniques Used:

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  • 88
    ATCC s marcescens ci
    Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. <t>marcescens</t> CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d e ) and intestinal colonization ( d , e f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p
    S Marcescens Ci, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s marcescens ci/product/ATCC
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    s marcescens ci - by Bioz Stars, 2022-09
    88/100 stars
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    Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d e ) and intestinal colonization ( d , e f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d e ) and intestinal colonization ( d , e f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques: Infection, Inhibition, Colony-forming Unit Assay

    Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques: Confocal Laser Scanning Microscopy, Activity Assay

    MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques: Concentration Assay, Cell Surface Hydrophobicity

    Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques:

    Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques: Nucleic Acid Electrophoresis, Molecular Weight, SDS Page, Staining, Mass Spectrometry

    Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques: Concentration Assay, Inhibition, Incubation

    Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.

    Journal: Scientific Reports

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    doi: 10.1038/s41598-017-16507-x

    Figure Lengend Snippet: Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.

    Article Snippet: Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1.

    Techniques: