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TCP11 overexpression induces the apoptosis of HeLa and SiHa cells. A and B : Apoptosis of HeLa and SiHa cells were detected by flow cytometry. C : Western blot was used to detect the protein expression <t>of</t> <t>caspase-3,</t> cleaved-caspase-3 and cleaved-PARP in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure . Data are presented as mean ± SD of at least 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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TCP11 overexpression induces the apoptosis of HeLa and SiHa cells. A and B : Apoptosis of HeLa and SiHa cells were detected by flow cytometry. C : Western blot was used to detect the protein expression <t>of</t> <t>caspase-3,</t> cleaved-caspase-3 and cleaved-PARP in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure . Data are presented as mean ± SD of at least 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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TCP11 overexpression induces the apoptosis of HeLa and SiHa cells. A and B : Apoptosis of HeLa and SiHa cells were detected by flow cytometry. C : Western blot was used to detect the protein expression of caspase-3, cleaved-caspase-3 and cleaved-PARP in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure . Data are presented as mean ± SD of at least 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Journal: BMC Cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: TCP11 overexpression induces the apoptosis of HeLa and SiHa cells. A and B : Apoptosis of HeLa and SiHa cells were detected by flow cytometry. C : Western blot was used to detect the protein expression of caspase-3, cleaved-caspase-3 and cleaved-PARP in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure . Data are presented as mean ± SD of at least 3 independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Anti-TCP11 (14606-1-AP) and anti-caspase-3 (8193T) were purchased from Proteintech (China).

Techniques: Over Expression, Flow Cytometry, Western Blot, Expressing, Infection