Journal: Cell Death & Disease

Article Title: In vivo self-assembly and delivery of VEGFR2 siRNA-encapsulated small extracellular vesicles for lung metastatic osteosarcoma therapy

doi: 10.1038/s41419-023-06159-3

Figure Lengend Snippet: A Flow chart of the experimental design. The OS lung metastasis model was constructed by injecting stable 143B cells labeled with firefly luciferase into the lateral tail vein of nude mice. Thirty days after injection, tumor-burdened mice were randomly divided into two groups for either survival analysis or tumor evaluation according to pulmonary tumor burden estimation by IVIS and micro-CT scans. Mice were then intravenously injected with the scrRNA circuit or anti-VEGFR2 circuit (10 mg/kg) or intragastrically administered 200 mg/kg apatinib every 2 days for a total of seven treatments. After treatment, the survival condition, tumor growth and VEGFR2 expression levels were evaluated. B Survival ratio of the mice treated with genetic circuits or apatinib ( n = 10 per group). C Representative IVIS images of lung metastases of mice pre- (day 30) and posttreatment (day 44) with the genetic circuits or apatinib. D Representative 3-D reconstructions of lung metastases pre- (day 30) and posttreatment (day 44) with the genetic circuits or apatinib. White arrow shows the tumor. E , F Representative images of collected lungs and hematoxylin and eosin (H&E) staining. White dots in E are light reflections, black arrow shows the tumor. G Western blot analysis of VEGFR2 protein levels in lung metastatic samples. Representative western blots are shown. H Heat-map showing the quantitation of VEGFR2 mRNA levels and protein levels in lung metastatic samples ( n = 6 per group). The detailed data quantifications are shown in Fig. . I , J Representative images of IHC staining for VEGFR2 and CD31 protein in lung metastasis sections. Scale bar: 100 μm.

Article Snippet: The human umbilical vein endothelial cell line HUVEC (RRID: CVCL_2959, female), mouse endothelial cell line EOMA (RRID: CVCL_3507), and human osteosarcoma cell line 143B (RRID: CVCL_2270, female) were purchased from American Type Culture Collection (ATCC).

Techniques: Construct, Labeling, Luciferase, Injection, Micro-CT, Expressing, Staining, Western Blot, Quantitation Assay, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: In vivo self-assembly and delivery of VEGFR2 siRNA-encapsulated small extracellular vesicles for lung metastatic osteosarcoma therapy

doi: 10.1038/s41419-023-06159-3

Figure Lengend Snippet: A Flow chart of the experimental design. The OS orthotopic xenograft tumor model was constructed by injecting stable 143B cells labeled with firefly luciferase into the tibial bone marrow cavity of NOD/SCID mice. Thirty days after injection, the tumor-bearing right legs of all mice were amputated aseptically. All mice were randomly divided into two groups for either survival analysis or tumor evaluation according to pulmonary tumor burden. Mice were then intravenously injected with the scrRNA circuit or anti-VEGFR2 circuit (10 mg/kg) or intragastrically administered 200 mg/kg apatinib every 2 days for a total of seven treatments. After treatment, the survival condition, tumor growth and VEGFR2 expression levels were evaluated. B Survival ratio of the mice treated with genetic circuits or apatinib ( n = 5 in scrRNA and apatinib group, n = 6 in anti-VEGFR2 group). C Representative IVIS images of lung metastases of mice pre- (day 30) and posttreatment (day 51) with the genetic circuits or apatinib. D Representative 3-D reconstructions of lung metastases posttreatment with the genetic circuits or apatinib on day 51. White arrow shows the tumor. E , F Representative images of collected lungs and H&E staining. White dots in E are light reflections, black arrow shows the tumor. G , H Representative images of IHC staining for VEGFR2 and CD31 protein in lung metastasis sections. Scale bar: 100 μm.

Article Snippet: The human umbilical vein endothelial cell line HUVEC (RRID: CVCL_2959, female), mouse endothelial cell line EOMA (RRID: CVCL_3507), and human osteosarcoma cell line 143B (RRID: CVCL_2270, female) were purchased from American Type Culture Collection (ATCC).

Techniques: Construct, Labeling, Luciferase, Injection, Expressing, Staining, Immunohistochemistry

Journal: Mitochondrion

Article Title: Quantification of mtDNA content in cultured cells by direct droplet digital PCR

doi: 10.1016/j.mito.2021.09.014

Figure Lengend Snippet: Sensitivity and accuracy of the dddPCR. A-D, mtCNs was determined in six standards generated by mixing lysates of wt and Rho-0 143B cells in four independent experiments. E, Accuracy of dddPCR in experiments A-D. Dotted line, 95% confidence interval of the linear regression. One-way ANOVA with post-hoc Tukey test. In each panel, only statistically not significant pairwise comparisons are shown.

Article Snippet: Cell growth and treatment In this study, we used human osteosarcoma 143B cells (ATCC CRL-8303), HeLa (human cervical carcinoma, ATCC CCL-2), and HT1080 (human fibrosarcoma, Cell culture collection of the University of California, Berkeley).

Techniques: Generated

Journal: Mitochondrion

Article Title: Quantification of mtDNA content in cultured cells by direct droplet digital PCR

doi: 10.1016/j.mito.2021.09.014

Figure Lengend Snippet: Horizontal stability of mtDNA in HeLa, 143B and HT1080 cells. Cells were cultivated for 6 days, and harvested for analysis on days 0, 2, 4, and 6.

Article Snippet: Cell growth and treatment In this study, we used human osteosarcoma 143B cells (ATCC CRL-8303), HeLa (human cervical carcinoma, ATCC CCL-2), and HT1080 (human fibrosarcoma, Cell culture collection of the University of California, Berkeley).

Techniques:

Journal: Mitochondrion

Article Title: Quantification of mtDNA content in cultured cells by direct droplet digital PCR

doi: 10.1016/j.mito.2021.09.014

Figure Lengend Snippet: Horizontal instability of mtCNs in cultured cells.

Article Snippet: Cell growth and treatment In this study, we used human osteosarcoma 143B cells (ATCC CRL-8303), HeLa (human cervical carcinoma, ATCC CCL-2), and HT1080 (human fibrosarcoma, Cell culture collection of the University of California, Berkeley).

Techniques: Cell Culture

Journal: Mitochondrion

Article Title: Quantification of mtDNA content in cultured cells by direct droplet digital PCR

doi: 10.1016/j.mito.2021.09.014

Figure Lengend Snippet: Effect of cell cycle progression on mtCN. Cells were treated as described in the Materials and Methods, and then their proliferation and mtCN were followed for indicated periods of time. A and B, Hela cells. C, 143B cells. N/A-not assayed. MitoC, mitomycin C; 100 Gy, X-irradiation, 100 Gy.

Article Snippet: Cell growth and treatment In this study, we used human osteosarcoma 143B cells (ATCC CRL-8303), HeLa (human cervical carcinoma, ATCC CCL-2), and HT1080 (human fibrosarcoma, Cell culture collection of the University of California, Berkeley).

Techniques: Irradiation

Journal: Biomedicines

Article Title: A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro

doi: 10.3390/biomedicines11061524

Figure Lengend Snippet: Schematic illustration of cinnamaldehyde polymeric prodrug micelles (mPEG- b -P(C7- co -CA)) inhibiting the proliferation, migration, and invasion of osteosarcoma 143B cells in vitro and leading to cell death.

Article Snippet: Human osteosarcoma cell lines 143B, MG63 and U2OS were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, In Vitro

Journal: Biomedicines

Article Title: A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro

doi: 10.3390/biomedicines11061524

Figure Lengend Snippet: Effect of mPEG- b -P(C7- co -CA) micelles on the proliferation of 143B cells detected by ( a ) CCK-8 and ( b , c ) EdU assays. ( d , e ) The expression levels of proliferation-related protein PCNA detected by Western blotting (scale bar 100 µm, * p < 0.05, *** p < 0.001, vs. the control group).

Article Snippet: Human osteosarcoma cell lines 143B, MG63 and U2OS were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: CCK-8 Assay, Expressing, Western Blot

Journal: Biomedicines

Article Title: A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro

doi: 10.3390/biomedicines11061524

Figure Lengend Snippet: ( a , b ) Effect of mPEG- b -P(C7- co -CA) micelles on the cell cycle of 143B cells was detected by flow cytometry (* p < 0.05, vs. the control group).

Article Snippet: Human osteosarcoma cell lines 143B, MG63 and U2OS were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry

Journal: Biomedicines

Article Title: A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro

doi: 10.3390/biomedicines11061524

Figure Lengend Snippet: Effect of mPEG- b -P(C7- co -CA) micelles on migration and invasion of 143B cells was detected by ( a , b ) wound-healing assays and ( c , d ) transwell assay. ( e , f ) The expression levels of migration- and invasion-related proteins N-cadherin, E-cadherin, MMP-2 and Snail detected by Western blotting (scale bar 100 µm, * p < 0.05, ** p < 0.01, *** p < 0.001, vs. the control group).

Article Snippet: Human osteosarcoma cell lines 143B, MG63 and U2OS were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Transwell Assay, Expressing, Western Blot

Journal: Biomedicines

Article Title: A Systematic Study of Anti-Osteosarcoma Mechanism of pH-Sensitive Charge-Conversion Cinnamaldehyde Polymeric Prodrug Micelles In Vitro

doi: 10.3390/biomedicines11061524

Figure Lengend Snippet: ( a , b ) The expression levels of PI3K/Akt signaling pathway-related proteins PI3K, Akt and p-Akt in 143B cells treated with mPEG- b -P(C7- co -CA) micelles were detected by western blotting (** p < 0.01, *** p < 0.001, vs. the control group).

Article Snippet: Human osteosarcoma cell lines 143B, MG63 and U2OS were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Western Blot