intracellulare  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    ATCC intracellulare
    Lung Histopathology of M . <t>intracellulare</t> infected mice. A: MI SM#42 represents the reactivation model. B: MI ATCC13950 and C: MI SM#30 are the regrowth models of the indicated M . intracellulare strains. (a) Untreated controls and (b) antibiotic-treated samples at 28 weeks post infection. Histopathological changes of the lungs with M . intracellulare infection at 35 weeks post infection with no immunosuppression (c), sulfasalazine treatment (d), and dexamethasone treatment (e). The scale bar represents 1 mm at ×10 magnification.
    Intracellulare, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intracellulare/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    intracellulare - by Bioz Stars, 2022-09
    95/100 stars

    Images

    1) Product Images from "Experimental Reactivation of Pulmonary Mycobacterium avium Complex Infection in a Modified Cornell-Like Murine Model"

    Article Title: Experimental Reactivation of Pulmonary Mycobacterium avium Complex Infection in a Modified Cornell-Like Murine Model

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0139251

    Lung Histopathology of M . intracellulare infected mice. A: MI SM#42 represents the reactivation model. B: MI ATCC13950 and C: MI SM#30 are the regrowth models of the indicated M . intracellulare strains. (a) Untreated controls and (b) antibiotic-treated samples at 28 weeks post infection. Histopathological changes of the lungs with M . intracellulare infection at 35 weeks post infection with no immunosuppression (c), sulfasalazine treatment (d), and dexamethasone treatment (e). The scale bar represents 1 mm at ×10 magnification.
    Figure Legend Snippet: Lung Histopathology of M . intracellulare infected mice. A: MI SM#42 represents the reactivation model. B: MI ATCC13950 and C: MI SM#30 are the regrowth models of the indicated M . intracellulare strains. (a) Untreated controls and (b) antibiotic-treated samples at 28 weeks post infection. Histopathological changes of the lungs with M . intracellulare infection at 35 weeks post infection with no immunosuppression (c), sulfasalazine treatment (d), and dexamethasone treatment (e). The scale bar represents 1 mm at ×10 magnification.

    Techniques Used: Histopathology, Infection, Mouse Assay

    Regrowth of Mycobacterium avium complex in the lungs of mice following immunosuppression. A: MAV104 and B: MAVSM#22 are the regrowth models of M . avium strains. C: MI ATCC13950 and D: MI SM#30 are the regrowth models of M . intracellulare strains. All graphs show a dramatic decrease in bacterial counts, although they remained detectable after antibiotic treatment with clarithromycin and rifampicin. The number of bacterial counts showed dramatic increase after dexamethasone and sulfasalazine treatment. The dashed line represents the detection limit of bacterial counts. The data are the median ± interquartile range (IQR). ** P
    Figure Legend Snippet: Regrowth of Mycobacterium avium complex in the lungs of mice following immunosuppression. A: MAV104 and B: MAVSM#22 are the regrowth models of M . avium strains. C: MI ATCC13950 and D: MI SM#30 are the regrowth models of M . intracellulare strains. All graphs show a dramatic decrease in bacterial counts, although they remained detectable after antibiotic treatment with clarithromycin and rifampicin. The number of bacterial counts showed dramatic increase after dexamethasone and sulfasalazine treatment. The dashed line represents the detection limit of bacterial counts. The data are the median ± interquartile range (IQR). ** P

    Techniques Used: Mouse Assay

    Reactivation of Mycobacterium avium complex in the lungs of mice following immunosuppression. Mice were infected with approximately 500–1,000 CFUs of each MAC strain for 10 weeks and treated for 6 weeks with clarithromycin and rifampicin. Following the antibiotic regimen, mice were treated with immunosuppressants. A: MAV SM#1 represents the reactivation model of M . avium strains. B: MI SM#42 represents the reactivation model of M . intracellulare strains. Both graphs depict a dramatic decrease in bacterial counts, which were undetectable after antibiotic treatment and a dramatic increase after sulfasalazine treatment. For A and B, CFUs at 35 weeks represent the sulfasalazine-treated group. C and D represent the bacterial counts of individual murine lungs infected with MAV SM#1 and MI SM#42, respectively. Each group exhibited different reactivation levels according to the immunosuppressant used. The dashed line represents the detection limit of bacterial counts. Red arrows represent undetectable bacilli in each mouse. For A and B, the data are the median ± interquartile range (IQR).
    Figure Legend Snippet: Reactivation of Mycobacterium avium complex in the lungs of mice following immunosuppression. Mice were infected with approximately 500–1,000 CFUs of each MAC strain for 10 weeks and treated for 6 weeks with clarithromycin and rifampicin. Following the antibiotic regimen, mice were treated with immunosuppressants. A: MAV SM#1 represents the reactivation model of M . avium strains. B: MI SM#42 represents the reactivation model of M . intracellulare strains. Both graphs depict a dramatic decrease in bacterial counts, which were undetectable after antibiotic treatment and a dramatic increase after sulfasalazine treatment. For A and B, CFUs at 35 weeks represent the sulfasalazine-treated group. C and D represent the bacterial counts of individual murine lungs infected with MAV SM#1 and MI SM#42, respectively. Each group exhibited different reactivation levels according to the immunosuppressant used. The dashed line represents the detection limit of bacterial counts. Red arrows represent undetectable bacilli in each mouse. For A and B, the data are the median ± interquartile range (IQR).

    Techniques Used: Mouse Assay, Infection

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    ATCC m intracellulare strains
    PRA (4% agarose gel stained with ethidium bromide) of M. avium ATCC 25291 (lanes 1), M. avium I (lanes 2), M. avium II (lanes 3), M. avium III (lanes 4), and M. <t>intracellulare</t> ATCC 13950 (lanes 5). Lane M, 25-bp DNA ladder (Gibco-BRL).
    M Intracellulare Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m intracellulare strains/product/ATCC
    Average 95 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    m intracellulare strains - by Bioz Stars, 2022-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    PRA (4% agarose gel stained with ethidium bromide) of M. avium ATCC 25291 (lanes 1), M. avium I (lanes 2), M. avium II (lanes 3), M. avium III (lanes 4), and M. intracellulare ATCC 13950 (lanes 5). Lane M, 25-bp DNA ladder (Gibco-BRL).

    Journal: Journal of Clinical Microbiology

    Article Title: Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR-Restriction Enzyme Analysis

    doi:

    Figure Lengend Snippet: PRA (4% agarose gel stained with ethidium bromide) of M. avium ATCC 25291 (lanes 1), M. avium I (lanes 2), M. avium II (lanes 3), M. avium III (lanes 4), and M. intracellulare ATCC 13950 (lanes 5). Lane M, 25-bp DNA ladder (Gibco-BRL).

    Article Snippet: Sequencing of the hsp65 fragment amplified with the TB11 and TB12 primers from the three PRA variants, M. avium ATCC 25291, and two M. intracellulare strains (one clinical isolate and ATCC 13950) was performed.

    Techniques: Agarose Gel Electrophoresis, Staining

    A comparative metabolic pathway analysis between M. tuberculosis H37Rv, M. intracellulare ATCC 13950, and M. indicus pranii reveals the presence of novel pathways in Mycobacterium tuberculosis that are not present in M. indicus pranii or M. intracellulare ATCC 13950. The comparative analysis of metabolic enzymes present in M. tuberculosis H37Rv, M. intracellulare ATCC 13950, and M. indicus pranii based on KEGG pathways are shown. (a) The unique pathways in M. tuberculosis H37Rv are shown in red. The common pathways between M. intracellulare ATCC 13950 and M. indicus pranii are shown in pink. The common pathways present in these three organisms are shown in black. Note that M. tuberculosis H37Rv has acquired alternate pathways (red) for its survival. There are few common pathways between M. intracellulare ATCC 13950 and M. indicus pranii (pink) that are absent in M. tuberculosis H37Rv. (b) A section (circled) of the lipid biosynthesis subpathway (glycerolipid metabolism) highlights the presence of an alternate enzyme (EC 3.1.1.3, Rv3097c) that performs molecular transformations in M. tuberculosis H37Rv. (c) A section (circled) of the lipid biosynthesis subpathway (fatty acid metabolism) highlights an alternate enzyme (FabH gene) present in M. intracellulare ATCC 13950 and M. indicus pranii but absent in M. tuberculosis H37Rv.

    Journal: mBio

    Article Title: Comparative Analyses of Nonpathogenic, Opportunistic, and Totally Pathogenic Mycobacteria Reveal Genomic and Biochemical Variabilities and Highlight the Survival Attributes of Mycobacterium tuberculosis

    doi: 10.1128/mBio.02020-14

    Figure Lengend Snippet: A comparative metabolic pathway analysis between M. tuberculosis H37Rv, M. intracellulare ATCC 13950, and M. indicus pranii reveals the presence of novel pathways in Mycobacterium tuberculosis that are not present in M. indicus pranii or M. intracellulare ATCC 13950. The comparative analysis of metabolic enzymes present in M. tuberculosis H37Rv, M. intracellulare ATCC 13950, and M. indicus pranii based on KEGG pathways are shown. (a) The unique pathways in M. tuberculosis H37Rv are shown in red. The common pathways between M. intracellulare ATCC 13950 and M. indicus pranii are shown in pink. The common pathways present in these three organisms are shown in black. Note that M. tuberculosis H37Rv has acquired alternate pathways (red) for its survival. There are few common pathways between M. intracellulare ATCC 13950 and M. indicus pranii (pink) that are absent in M. tuberculosis H37Rv. (b) A section (circled) of the lipid biosynthesis subpathway (glycerolipid metabolism) highlights the presence of an alternate enzyme (EC 3.1.1.3, Rv3097c) that performs molecular transformations in M. tuberculosis H37Rv. (c) A section (circled) of the lipid biosynthesis subpathway (fatty acid metabolism) highlights an alternate enzyme (FabH gene) present in M. intracellulare ATCC 13950 and M. indicus pranii but absent in M. tuberculosis H37Rv.

    Article Snippet: Analysis of metabolic enzymes was carried out based on the IUBMB EC numbers ( ) in the KEGG database ( ) (accessed in December 2012) for M. indicus pranii (387 EC enzymes), M. intracellulare ATCC 13950 (394 EC), M. avium 104 (413 EC), and M. tuberculosis (396 EC) genomes ( ).

    Techniques:

    Comparative genomics of selected mycobacterial genomes. The genomes of Mycobacterium indicus pranii (shown in green; an NP), Mycobacterium intracellulare ATCC 13950 (orange; an OP), and Mycobacterium tuberculosis H37Rv (pink; a TP) were selected for comparative genomic analyses. We used BLASTp, with a cutoff of 20% identity and e value of 10e–4, to determine the number of homologous protein-coding genes common between them (shown as edge labels between the nodes). The arrowhead represents the query genome, whereas the arrow tail represents the subject genome.

    Journal: mBio

    Article Title: Comparative Analyses of Nonpathogenic, Opportunistic, and Totally Pathogenic Mycobacteria Reveal Genomic and Biochemical Variabilities and Highlight the Survival Attributes of Mycobacterium tuberculosis

    doi: 10.1128/mBio.02020-14

    Figure Lengend Snippet: Comparative genomics of selected mycobacterial genomes. The genomes of Mycobacterium indicus pranii (shown in green; an NP), Mycobacterium intracellulare ATCC 13950 (orange; an OP), and Mycobacterium tuberculosis H37Rv (pink; a TP) were selected for comparative genomic analyses. We used BLASTp, with a cutoff of 20% identity and e value of 10e–4, to determine the number of homologous protein-coding genes common between them (shown as edge labels between the nodes). The arrowhead represents the query genome, whereas the arrow tail represents the subject genome.

    Article Snippet: Analysis of metabolic enzymes was carried out based on the IUBMB EC numbers ( ) in the KEGG database ( ) (accessed in December 2012) for M. indicus pranii (387 EC enzymes), M. intracellulare ATCC 13950 (394 EC), M. avium 104 (413 EC), and M. tuberculosis (396 EC) genomes ( ).

    Techniques:

    The enzymatic similarities between M. indicus pranii (MIP), M. intracellulare ATCC 13950 (MIA), M. avium 104 (MYCA1), and M. tuberculosis H37Rv (MYCTU) highlight interesting enzymatic plasticity properties. M. intracellulare ATCC 13950 (OP; orange) shares three enzymes (EC 1.8.7.1, sulfite reductase [ferredoxin]; EC 2.7.1.6, galactokinase [phosphorylating]; EC 5.4.2.8, phosphor mannose mutase) with M. avium 104 (OP; blue) and M. tuberculosis H37Rv (TP; red), which are absent in M. indicus pranii (NP; green). M. intracellulare ATCC 13950 and M. indicus pranii share 17 enzymes between them that are absent in M. tuberculosis H37Rv (a), while they share 12 enzymes between them that are absent in M. avium 104 (b).

    Journal: mBio

    Article Title: Comparative Analyses of Nonpathogenic, Opportunistic, and Totally Pathogenic Mycobacteria Reveal Genomic and Biochemical Variabilities and Highlight the Survival Attributes of Mycobacterium tuberculosis

    doi: 10.1128/mBio.02020-14

    Figure Lengend Snippet: The enzymatic similarities between M. indicus pranii (MIP), M. intracellulare ATCC 13950 (MIA), M. avium 104 (MYCA1), and M. tuberculosis H37Rv (MYCTU) highlight interesting enzymatic plasticity properties. M. intracellulare ATCC 13950 (OP; orange) shares three enzymes (EC 1.8.7.1, sulfite reductase [ferredoxin]; EC 2.7.1.6, galactokinase [phosphorylating]; EC 5.4.2.8, phosphor mannose mutase) with M. avium 104 (OP; blue) and M. tuberculosis H37Rv (TP; red), which are absent in M. indicus pranii (NP; green). M. intracellulare ATCC 13950 and M. indicus pranii share 17 enzymes between them that are absent in M. tuberculosis H37Rv (a), while they share 12 enzymes between them that are absent in M. avium 104 (b).

    Article Snippet: Analysis of metabolic enzymes was carried out based on the IUBMB EC numbers ( ) in the KEGG database ( ) (accessed in December 2012) for M. indicus pranii (387 EC enzymes), M. intracellulare ATCC 13950 (394 EC), M. avium 104 (413 EC), and M. tuberculosis (396 EC) genomes ( ).

    Techniques:

    Phylogenetic tree of 925 SNPs from the optimized 7-gene set ( fusA, secA, 16S-23S rRNA ITS , rpoB, hsp65, 16S rRNA, 23S rRNA ) of MAC isolates using the maximum likelihood method. All 49 strains were identified as either Mycobacterium intracellulare or Mycobacterium avium subsp. avium. This bootstrap consensus tree was inferred from 1000 replicates. Blue circles represent bootstrap values and the size of each circle is proportional to its value (the largest blue circle indicates a value of 100%). M. avium subsp. avium, M. avium subsp. hominissuis, M. avium subsp. paratuberculosis and M. intracellulare ATCC 13950 (accession numbers CP028731, CP018363, NC_002944 and CP003322, respectively) were used as reference strains. (D = Disseminated, P = Pulmonary, S = Skin, T = True, C = Colonization, E = Extra-pulmonary, Pink colour = M. avium , Dark pink = M. intracellulare ). C1 = cluster 1 (Patient 11.2 and 12.2), C2 = cluster 2 (Patient 5.1–5.2, 13.1 and 12.1). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: One Health

    Article Title: High rate of reinfection and possible transmission of Mycobacterium avium complex in Northeast Thailand

    doi: 10.1016/j.onehlt.2022.100374

    Figure Lengend Snippet: Phylogenetic tree of 925 SNPs from the optimized 7-gene set ( fusA, secA, 16S-23S rRNA ITS , rpoB, hsp65, 16S rRNA, 23S rRNA ) of MAC isolates using the maximum likelihood method. All 49 strains were identified as either Mycobacterium intracellulare or Mycobacterium avium subsp. avium. This bootstrap consensus tree was inferred from 1000 replicates. Blue circles represent bootstrap values and the size of each circle is proportional to its value (the largest blue circle indicates a value of 100%). M. avium subsp. avium, M. avium subsp. hominissuis, M. avium subsp. paratuberculosis and M. intracellulare ATCC 13950 (accession numbers CP028731, CP018363, NC_002944 and CP003322, respectively) were used as reference strains. (D = Disseminated, P = Pulmonary, S = Skin, T = True, C = Colonization, E = Extra-pulmonary, Pink colour = M. avium , Dark pink = M. intracellulare ). C1 = cluster 1 (Patient 11.2 and 12.2), C2 = cluster 2 (Patient 5.1–5.2, 13.1 and 12.1). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Reads from each isolate were mapped to the reference genome of M. intracellulare ATCC 13950 (SRA accession no. CP003322) using BWA-MEM version 0.7.17 [ ].

    Techniques:

    Concentration-dependent intracellular activities of CFZ in MAC-infected BMDMs. BMDMs were infected with (A) M. avium ATCC 700898 or (B) M. intracellulare ATCC 13950 and treated with the indicated dose of CFZ. (C) BMDMs were infected with M. avium ATCC 700898, M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 5 mg/L CFZ or 10 mg/L CLR. After 72 h of cultivation, bacterial CFUs were enumerated by plating serially diluted cell lysates on 7H10-OADC agar plates. Each experiment was repeated at least twice independently with triplicate wells; the results of a representative experiment are shown. Each dot represents the mean value ± S.D. of duplicate or triplicate wells, with four spots applied per well. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. *** p

    Journal: Frontiers in Microbiology

    Article Title: A Clofazimine-Containing Regimen Confers Improved Treatment Outcomes in Macrophages and in a Murine Model of Chronic Progressive Pulmonary Infection Caused by the Mycobacterium avium Complex

    doi: 10.3389/fmicb.2020.626216

    Figure Lengend Snippet: Concentration-dependent intracellular activities of CFZ in MAC-infected BMDMs. BMDMs were infected with (A) M. avium ATCC 700898 or (B) M. intracellulare ATCC 13950 and treated with the indicated dose of CFZ. (C) BMDMs were infected with M. avium ATCC 700898, M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 5 mg/L CFZ or 10 mg/L CLR. After 72 h of cultivation, bacterial CFUs were enumerated by plating serially diluted cell lysates on 7H10-OADC agar plates. Each experiment was repeated at least twice independently with triplicate wells; the results of a representative experiment are shown. Each dot represents the mean value ± S.D. of duplicate or triplicate wells, with four spots applied per well. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. *** p

    Article Snippet: Next, BMDMs were infected with M. avium ATCC 700898, M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 5 mg/L CFZ or 10 mg/L CLR, and intracellular activity was assessed at 72 h post-infection ( ).

    Techniques: Concentration Assay, Infection, MANN-WHITNEY

    Intracellular anti-MAC activities of first-line drugs in MAC-infected BMDMs. (A) BMDMs were infected with M. avium ATCC 700898 and treated with the indicated doses of CLR, EMB, and RIF. Unless otherwise indicated, all experiments investigating the intracellular activities of drugs in BMDMs were evaluated at 72 h post-infection by plating serially diluted cell lysates onto 7H10-OADC agar plates. (B) BMDMs were infected with M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 10 mg/L RIF. (C) BMDMs were infected with M. tuberculosis H37Rv and treated with 1 mg/L RIF as a control experiment. BMDMs were infected with (D) M. avium ATCC 700898 or (E) M. intracellulare ATCC 13950 and treated with the indicated doses of RIF. Each experiment was repeated at least twice independently with duplicate or triplicate wells; the results of a representative experiment are shown. Each dot represents the mean value ± S.D. of duplicate or triplicate wells, with four spots applied per well. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. *** p

    Journal: Frontiers in Microbiology

    Article Title: A Clofazimine-Containing Regimen Confers Improved Treatment Outcomes in Macrophages and in a Murine Model of Chronic Progressive Pulmonary Infection Caused by the Mycobacterium avium Complex

    doi: 10.3389/fmicb.2020.626216

    Figure Lengend Snippet: Intracellular anti-MAC activities of first-line drugs in MAC-infected BMDMs. (A) BMDMs were infected with M. avium ATCC 700898 and treated with the indicated doses of CLR, EMB, and RIF. Unless otherwise indicated, all experiments investigating the intracellular activities of drugs in BMDMs were evaluated at 72 h post-infection by plating serially diluted cell lysates onto 7H10-OADC agar plates. (B) BMDMs were infected with M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 10 mg/L RIF. (C) BMDMs were infected with M. tuberculosis H37Rv and treated with 1 mg/L RIF as a control experiment. BMDMs were infected with (D) M. avium ATCC 700898 or (E) M. intracellulare ATCC 13950 and treated with the indicated doses of RIF. Each experiment was repeated at least twice independently with duplicate or triplicate wells; the results of a representative experiment are shown. Each dot represents the mean value ± S.D. of duplicate or triplicate wells, with four spots applied per well. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. *** p

    Article Snippet: Next, BMDMs were infected with M. avium ATCC 700898, M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 5 mg/L CFZ or 10 mg/L CLR, and intracellular activity was assessed at 72 h post-infection ( ).

    Techniques: Infection, MANN-WHITNEY

    Comparative evaluation of the intracellular activities of drug combinations for the standard regimen and for the CFZ-containing regimen against a variety of MAC strains in BMDMs. BMDMs were infected with each MAC strain and treated with the drug regimen at 1 × MIC according to the data in Table 1 . (A) M. avium ATCC 700898 and M. intracellulare ATCC 13950, (B) M. avium SMC #1 and M. avium SMC #7 and (C) M. intracellulare SMC #8 and M. intracellulare SMC #11 were assessed at 72 h post-infection by plating serially diluted cell lysates on 7H10-OADC agar plates. Each experiment was repeated at least twice independently with triplicate wells; the results of a representative experiment are shown. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. *** p

    Journal: Frontiers in Microbiology

    Article Title: A Clofazimine-Containing Regimen Confers Improved Treatment Outcomes in Macrophages and in a Murine Model of Chronic Progressive Pulmonary Infection Caused by the Mycobacterium avium Complex

    doi: 10.3389/fmicb.2020.626216

    Figure Lengend Snippet: Comparative evaluation of the intracellular activities of drug combinations for the standard regimen and for the CFZ-containing regimen against a variety of MAC strains in BMDMs. BMDMs were infected with each MAC strain and treated with the drug regimen at 1 × MIC according to the data in Table 1 . (A) M. avium ATCC 700898 and M. intracellulare ATCC 13950, (B) M. avium SMC #1 and M. avium SMC #7 and (C) M. intracellulare SMC #8 and M. intracellulare SMC #11 were assessed at 72 h post-infection by plating serially diluted cell lysates on 7H10-OADC agar plates. Each experiment was repeated at least twice independently with triplicate wells; the results of a representative experiment are shown. The Mann–Whitney test was used to evaluate significance, and the results are represented as the mean value ± S.D. *** p

    Article Snippet: Next, BMDMs were infected with M. avium ATCC 700898, M. intracellulare ATCC 13950 or M. avium SMC #7 and treated with 5 mg/L CFZ or 10 mg/L CLR, and intracellular activity was assessed at 72 h post-infection ( ).

    Techniques: Infection, MANN-WHITNEY