patellamide a  (ATCC)

Journal: iScience

Article Title: RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle

doi: 10.1016/j.isci.2024.111400

Figure Lengend Snippet: PSAP-mediated cyclical splicing is controlled by RNPS1 protein level through the ubiquitin-proteasome pathway (A) HEK293 cells were cultured for 4 h and 8 h with proteasome inhibitor MG132 and the protein levels were analyzed by Western blotting using the antibody against each ASAP/PSAP protein. A common solvent, dimethyl sulfoxide (DMSO), was added to the medium (final concentration at 0.1%). The RNPS1 band on the Western blots was quantified and the relative values were standardized to that at the start time, 0 h (right graph). Means ± SE are given for three independent experiments and Welch’s t-test values were calculated (∗ p < 0.05, ∗∗ p < 0.005, n.s. p > 0.05). (B) A schematic model of RNPS1-mediated periodic splicing during cell cycle. RNPS1 protein, but neither PNN nor SAP18 protein, in PSAP plays an essential role to control periodic splicing over the cell cycle progression. The ubiquitin-mediated proteolysis of RNPS1 also destabilizes SAP18 that induces dissociation of PSAP complex, leading to the splicing defect.

Article Snippet: The following antibodies were used to detect target proteins: anti-RNPS1 (1:750 dilution), anti-ACIN1 (1:1500 dilution; Cells Signaling Technology), anti-PNN (1:1500 dilution; Sigma-Aldrich), anti-SAP18 (1:1500 dilution; Proteintech), anti-AURKB (1:1500 dilution; Cells Signaling Technology) and anti-GAPDH (1:1500 dilution; MBL Life Science) antibodies.

Techniques: Cell Culture, Western Blot, Solvent, Concentration Assay, Control

Journal: iScience

Article Title: RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle

doi: 10.1016/j.isci.2024.111400

Figure Lengend Snippet:

Article Snippet: The following antibodies were used to detect target proteins: anti-RNPS1 (1:750 dilution), anti-ACIN1 (1:1500 dilution; Cells Signaling Technology), anti-PNN (1:1500 dilution; Sigma-Aldrich), anti-SAP18 (1:1500 dilution; Proteintech), anti-AURKB (1:1500 dilution; Cells Signaling Technology) and anti-GAPDH (1:1500 dilution; MBL Life Science) antibodies.

Techniques: Virus, Recombinant, Transfection, Software

Journal: iScience

Article Title: RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle

doi: 10.1016/j.isci.2024.111400

Figure Lengend Snippet: PSAP-mediated cyclical splicing is controlled by RNPS1 protein level through the ubiquitin-proteasome pathway (A) HEK293 cells were cultured for 4 h and 8 h with proteasome inhibitor MG132 and the protein levels were analyzed by Western blotting using the antibody against each ASAP/PSAP protein. A common solvent, dimethyl sulfoxide (DMSO), was added to the medium (final concentration at 0.1%). The RNPS1 band on the Western blots was quantified and the relative values were standardized to that at the start time, 0 h (right graph). Means ± SE are given for three independent experiments and Welch’s t-test values were calculated (∗ p < 0.05, ∗∗ p < 0.005, n.s. p > 0.05). (B) A schematic model of RNPS1-mediated periodic splicing during cell cycle. RNPS1 protein, but neither PNN nor SAP18 protein, in PSAP plays an essential role to control periodic splicing over the cell cycle progression. The ubiquitin-mediated proteolysis of RNPS1 also destabilizes SAP18 that induces dissociation of PSAP complex, leading to the splicing defect.

Article Snippet: Rabbit anti-SAP18 , Proteintech , #13841-1-AP.

Techniques: Cell Culture, Western Blot, Solvent, Concentration Assay, Control

Journal: iScience

Article Title: RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle

doi: 10.1016/j.isci.2024.111400

Figure Lengend Snippet:

Article Snippet: Rabbit anti-SAP18 , Proteintech , #13841-1-AP.

Techniques: Virus, Recombinant, Transfection, Software