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Effect of poliovirus on glycolytic and respiratory activities of GBM cell lines. U-251 MG ( a , c ) or DBTRG-05MG ( b , d ) cells maintained in DMEM or Plasmax media were infected with PV at MOI 1, and extracellular acidification (ECAR) reflecting <t>glycolysis</t> ( a , b ) and oxygen consumption rate (OCR) reflecting respiratory activity ( c , d ) were measured by Seahorse technology using GlycoStress and MitoStress assays. Results are presented as mean ± SD.
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Effect of poliovirus on glycolytic and respiratory activities of GBM cell lines. U-251 MG ( a , c ) or DBTRG-05MG ( b , d ) cells maintained in DMEM or Plasmax media were infected with PV at MOI 1, and extracellular acidification (ECAR) reflecting <t>glycolysis</t> ( a , b ) and oxygen consumption rate (OCR) reflecting respiratory activity ( c , d ) were measured by Seahorse technology using GlycoStress and MitoStress assays. Results are presented as mean ± SD.
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Effect of poliovirus on glycolytic and respiratory activities of GBM cell lines. U-251 MG ( a , c ) or DBTRG-05MG ( b , d ) cells maintained in DMEM or Plasmax media were infected with PV at MOI 1, and extracellular acidification (ECAR) reflecting glycolysis ( a , b ) and oxygen consumption rate (OCR) reflecting respiratory activity ( c , d ) were measured by Seahorse technology using GlycoStress and MitoStress assays. Results are presented as mean ± SD.

Journal: International Journal of Molecular Sciences

Article Title: Metabolic Imprint of Poliovirus on Glioblastoma Cells and Its Role in Virus Replication and Cytopathic Activity

doi: 10.3390/ijms26157346

Figure Lengend Snippet: Effect of poliovirus on glycolytic and respiratory activities of GBM cell lines. U-251 MG ( a , c ) or DBTRG-05MG ( b , d ) cells maintained in DMEM or Plasmax media were infected with PV at MOI 1, and extracellular acidification (ECAR) reflecting glycolysis ( a , b ) and oxygen consumption rate (OCR) reflecting respiratory activity ( c , d ) were measured by Seahorse technology using GlycoStress and MitoStress assays. Results are presented as mean ± SD.

Article Snippet: AR-C155858 , MCT1 and MCT2 inhibitor , Glycolysis , MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Infection, Activity Assay