klebsiella oxytoca  (ATCC)


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    ATCC klebsiella oxytoca
    ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived <t>Klebsiella</t> bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. <t>oxytoca/michiganensis</t> . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .
    Klebsiella Oxytoca, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Characterisation of the symbionts in the Mediterranean fruit fly gut"

    Article Title: Characterisation of the symbionts in the Mediterranean fruit fly gut

    Journal: Microbial Genomics

    doi: 10.1099/mgen.0.000801

    ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .
    Figure Legend Snippet: ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .

    Techniques Used: Derivative Assay, Isolation

    2) Product Images from "Characterisation of the symbionts in the Mediterranean fruit fly gut"

    Article Title: Characterisation of the symbionts in the Mediterranean fruit fly gut

    Journal: Microbial Genomics

    doi: 10.1099/mgen.0.000801

    ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .
    Figure Legend Snippet: ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .

    Techniques Used: Derivative Assay, Isolation

    3) Product Images from "Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity"

    Article Title: Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity

    Journal: Polymers

    doi: 10.3390/polym14040771

    Minimum inhibitory concentration (MIC) of CTX and C-AuNPs against ( A ) Escherichia coli ; ( B ) Klebsiella oxytoca ; ( C ) Pseudomonas aeruginosa. ( D ) Staphylococcus aureus. The data represent the means ± standard errors of three independent experiments.
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) of CTX and C-AuNPs against ( A ) Escherichia coli ; ( B ) Klebsiella oxytoca ; ( C ) Pseudomonas aeruginosa. ( D ) Staphylococcus aureus. The data represent the means ± standard errors of three independent experiments.

    Techniques Used: Concentration Assay

    4) Product Images from "Bacterial Indole as a Multifunctional Regulator of Klebsiella oxytoca Complex Enterotoxicity"

    Article Title: Bacterial Indole as a Multifunctional Regulator of Klebsiella oxytoca Complex Enterotoxicity

    Journal: mBio

    doi: 10.1128/mbio.03752-21

    Enterotoxicity of K. oxytoca complex is differentially regulated by glucose and indole. (A) Filtered supernatants from K. grimontii strain UCH-1 cultures, grown for 18 h in LB medium, LBG, or LBG plus 1.0 mM indole, were applied to T84 enterocytes. Shown are percentages of sub-G1 apoptotic cells following 72 h of exposure as determined by flow cytometry. Concentrations of tilimycin and tilivalline in the culture supernatants were determined by LC-MS ( n = 3 to 8). The data are representative images or mean values ± standard errors of the means. (B) Growth comparison of indole-producing K. oxytoca AHC-6, the indole-deficient AHC-6 Δ tnaA , and complementing strain AHC-6 Δ tnaA (pTnaA) grown in CASO medium at 37°C ( n = 3). The data represent mean values ± SD. (C) Cytotoxicity of filtered supernatants collected from cells used for panel B on HeLa cells ( n = 3; measured in triplicates). Shown are reciprocal values (means ± SD) of surviving HeLa cells after treatment with 1/27 dilutions of supernatants. Statistical comparison (Mann-Whitney) of AHC-6 and AHC-6 Δ tnaA was done for each time point. * , P
    Figure Legend Snippet: Enterotoxicity of K. oxytoca complex is differentially regulated by glucose and indole. (A) Filtered supernatants from K. grimontii strain UCH-1 cultures, grown for 18 h in LB medium, LBG, or LBG plus 1.0 mM indole, were applied to T84 enterocytes. Shown are percentages of sub-G1 apoptotic cells following 72 h of exposure as determined by flow cytometry. Concentrations of tilimycin and tilivalline in the culture supernatants were determined by LC-MS ( n = 3 to 8). The data are representative images or mean values ± standard errors of the means. (B) Growth comparison of indole-producing K. oxytoca AHC-6, the indole-deficient AHC-6 Δ tnaA , and complementing strain AHC-6 Δ tnaA (pTnaA) grown in CASO medium at 37°C ( n = 3). The data represent mean values ± SD. (C) Cytotoxicity of filtered supernatants collected from cells used for panel B on HeLa cells ( n = 3; measured in triplicates). Shown are reciprocal values (means ± SD) of surviving HeLa cells after treatment with 1/27 dilutions of supernatants. Statistical comparison (Mann-Whitney) of AHC-6 and AHC-6 Δ tnaA was done for each time point. * , P

    Techniques Used: Flow Cytometry, Liquid Chromatography with Mass Spectroscopy, MANN-WHITNEY

    Indole represses tilimycin synthesis in vivo . C57BL/6NRj mice were infected with K. oxytoca AHC-6 ( n = 4) or the indole-deficient Δ tnaA strain ( n = 6). (A) K. oxytoca colonization levels were determined by plating fecal samples on CASO-kanamycin agar. Shown are CFU per gram of feces over time for individual mice colonized with AHC-6 or the Δ tnaA strain; black horizontal bars represent the geometric means. The inset shows a simplified comparison of geometric means for both strains over time in the same scale. (B) Fecal tilimycin and indole values were determined by LC-MS daily for each mouse. Plotted are mean ± SEM tilimycin and indole values for each group (AHC-6; Δ tnaA ) and day. Dashed black lines represent the limit of quantification for indole (LOQ) and simultaneously indicate lack of quantifiable indole values for the Δ tnaA group. (C) Statistical significance of differences in total tilimycin amounts (area under the curve) generated by AHC-6 and the Δ tnaA mutant before (days 0 to 2) and after (days 2 to 6) indole production was determined by Mann-Whitney U test. * , P = 0.0333.
    Figure Legend Snippet: Indole represses tilimycin synthesis in vivo . C57BL/6NRj mice were infected with K. oxytoca AHC-6 ( n = 4) or the indole-deficient Δ tnaA strain ( n = 6). (A) K. oxytoca colonization levels were determined by plating fecal samples on CASO-kanamycin agar. Shown are CFU per gram of feces over time for individual mice colonized with AHC-6 or the Δ tnaA strain; black horizontal bars represent the geometric means. The inset shows a simplified comparison of geometric means for both strains over time in the same scale. (B) Fecal tilimycin and indole values were determined by LC-MS daily for each mouse. Plotted are mean ± SEM tilimycin and indole values for each group (AHC-6; Δ tnaA ) and day. Dashed black lines represent the limit of quantification for indole (LOQ) and simultaneously indicate lack of quantifiable indole values for the Δ tnaA group. (C) Statistical significance of differences in total tilimycin amounts (area under the curve) generated by AHC-6 and the Δ tnaA mutant before (days 0 to 2) and after (days 2 to 6) indole production was determined by Mann-Whitney U test. * , P = 0.0333.

    Techniques Used: In Vivo, Mouse Assay, Infection, Liquid Chromatography with Mass Spectroscopy, Generated, Mutagenesis, MANN-WHITNEY

    Glucose and indole reciprocally regulate cytotoxin synthesis by K. oxytoca strain AHC-6 and the AHC-6Δ tnaA mutant. (A) Comparison of UCH-1 growth with AHC-6 and AHC-6Δ tnaA in LB medium with glucose (LBG) at 37°C for 6 h ( n = 3 to 7). (B) Analysis of tilimycin and tilivalline metabolite levels from 18-h culture supernatants of AHC-6 and AHC-6 Δ tnaA grown in LB medium with or without added glucose determined by LC-MS. ND, not detected ( n = 3 to 8). (C) Effect of indole (1 mM) on npsA and npsB expression in AHC-6 and AHC-6 Δ tnaA grown in LBG. Transcript copy numbers for npsA and npsB (per 100 copies of recA transcript) in samples obtained at postexponential (6 h) growth phase ( n = 6 or 7) were determined by RT-qPCR. (D) Effect of indole (1 mM) on tilimycin and tilivalline metabolite levels from 18-h culture supernatants of AHC-6 and AHC-6 Δ tnaA grown in LBG determined by LC-MS ( n = 3 to 7). The data represent mean values ± standard errors of the means. Statistical analysis was done by Mann-Whitney U test. In samples where no tilivalline was detected, the detection limit by LC-MS was used for statistics. Where stated, “vehicle” means addition of DMF (0.1%). * , P ≤ 0.05; * * , P ≤ 0.01.
    Figure Legend Snippet: Glucose and indole reciprocally regulate cytotoxin synthesis by K. oxytoca strain AHC-6 and the AHC-6Δ tnaA mutant. (A) Comparison of UCH-1 growth with AHC-6 and AHC-6Δ tnaA in LB medium with glucose (LBG) at 37°C for 6 h ( n = 3 to 7). (B) Analysis of tilimycin and tilivalline metabolite levels from 18-h culture supernatants of AHC-6 and AHC-6 Δ tnaA grown in LB medium with or without added glucose determined by LC-MS. ND, not detected ( n = 3 to 8). (C) Effect of indole (1 mM) on npsA and npsB expression in AHC-6 and AHC-6 Δ tnaA grown in LBG. Transcript copy numbers for npsA and npsB (per 100 copies of recA transcript) in samples obtained at postexponential (6 h) growth phase ( n = 6 or 7) were determined by RT-qPCR. (D) Effect of indole (1 mM) on tilimycin and tilivalline metabolite levels from 18-h culture supernatants of AHC-6 and AHC-6 Δ tnaA grown in LBG determined by LC-MS ( n = 3 to 7). The data represent mean values ± standard errors of the means. Statistical analysis was done by Mann-Whitney U test. In samples where no tilivalline was detected, the detection limit by LC-MS was used for statistics. Where stated, “vehicle” means addition of DMF (0.1%). * , P ≤ 0.05; * * , P ≤ 0.01.

    Techniques Used: Mutagenesis, Liquid Chromatography with Mass Spectroscopy, Expressing, Quantitative RT-PCR, MANN-WHITNEY

    5) Product Images from "Subtractive Proteomics and Immuno-informatics Approaches for Multi-peptide Vaccine Prediction Against Klebsiella oxytoca and Validation Through In Silico Expression"

    Article Title: Subtractive Proteomics and Immuno-informatics Approaches for Multi-peptide Vaccine Prediction Against Klebsiella oxytoca and Validation Through In Silico Expression

    Journal: International Journal of Peptide Research and Therapeutics

    doi: 10.1007/s10989-021-10283-z

    Three dimensional structure of docked complex of human TLR2 and Klebsiella oxytoca vaccine construct (vaccine construct: purple and solid, HTLR2: green)
    Figure Legend Snippet: Three dimensional structure of docked complex of human TLR2 and Klebsiella oxytoca vaccine construct (vaccine construct: purple and solid, HTLR2: green)

    Techniques Used: Construct

    Two dimensional interactions in docked complex of human TLR2 and Klebsiella oxytoca vaccine construct
    Figure Legend Snippet: Two dimensional interactions in docked complex of human TLR2 and Klebsiella oxytoca vaccine construct

    Techniques Used: Construct

    Disulfide engineered structure of Klebsiella oxytoca vaccine construct
    Figure Legend Snippet: Disulfide engineered structure of Klebsiella oxytoca vaccine construct

    Techniques Used: Construct

    Amino acid sequence of Klebsiella oxytoca vaccine construct with cysteine mutations
    Figure Legend Snippet: Amino acid sequence of Klebsiella oxytoca vaccine construct with cysteine mutations

    Techniques Used: Sequencing, Construct

    The engineered Klebsiella oxytoca vaccine construct map for cloning
    Figure Legend Snippet: The engineered Klebsiella oxytoca vaccine construct map for cloning

    Techniques Used: Construct, Clone Assay

    Thermostability curve of Klebsiella oxytoca vaccine protein after disulfide engineering
    Figure Legend Snippet: Thermostability curve of Klebsiella oxytoca vaccine protein after disulfide engineering

    Techniques Used:

    Subtractive proteomic schema for vaccine construction against Klebsiella oxytoca
    Figure Legend Snippet: Subtractive proteomic schema for vaccine construction against Klebsiella oxytoca

    Techniques Used:

    Amino acid sequence of vaccine construct for Klebsiella oxytoca
    Figure Legend Snippet: Amino acid sequence of vaccine construct for Klebsiella oxytoca

    Techniques Used: Sequencing, Construct

    The cloned pET28a (+) vector showing Klebsiella oxytoca vaccine construct as red region
    Figure Legend Snippet: The cloned pET28a (+) vector showing Klebsiella oxytoca vaccine construct as red region

    Techniques Used: Clone Assay, Plasmid Preparation, Construct

    Predicted 3D structure of Klebsiella oxytoca vaccine construct
    Figure Legend Snippet: Predicted 3D structure of Klebsiella oxytoca vaccine construct

    Techniques Used: Construct

    Thermostability curve of Klebsiella oxytoca vaccine protein
    Figure Legend Snippet: Thermostability curve of Klebsiella oxytoca vaccine protein

    Techniques Used:

    6) Product Images from "Characterisation of the symbionts in the Mediterranean fruitfly gut"

    Article Title: Characterisation of the symbionts in the Mediterranean fruitfly gut

    Journal: bioRxiv

    doi: 10.1101/2021.06.09.447743

    ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator ( Figueras et al 2014 ), with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. (2014) , Medkleb is conspecific with twelve strains which have been classified as both K. oxytoca and K. michiganensis .
    Figure Legend Snippet: ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator ( Figueras et al 2014 ), with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. (2014) , Medkleb is conspecific with twelve strains which have been classified as both K. oxytoca and K. michiganensis .

    Techniques Used: Derivative Assay, Isolation

    7) Product Images from "ZrO2–ZnO Nanoparticles as Antibacterial Agents"

    Article Title: ZrO2–ZnO Nanoparticles as Antibacterial Agents

    Journal: ACS Omega

    doi: 10.1021/acsomega.9b02527

    Inhibition zone (mm) formed by the different nanoparticles in the disc diffusion test on (a) B. subtilis, (b) S. aureus, (c) S. mutans, (d) E. coli, (e) K. oxytoca, and (f) P. aeruginosa .
    Figure Legend Snippet: Inhibition zone (mm) formed by the different nanoparticles in the disc diffusion test on (a) B. subtilis, (b) S. aureus, (c) S. mutans, (d) E. coli, (e) K. oxytoca, and (f) P. aeruginosa .

    Techniques Used: Inhibition, Diffusion-based Assay

    Growth characteristics and the statistical evaluation of the growth characteristics, respectively, formed by the nanoparticles on (a,b) E. coli, (c,d) K. oxytoca, and (e,f) P. aeruginosa .
    Figure Legend Snippet: Growth characteristics and the statistical evaluation of the growth characteristics, respectively, formed by the nanoparticles on (a,b) E. coli, (c,d) K. oxytoca, and (e,f) P. aeruginosa .

    Techniques Used:

    Inhibition zone (mm) formed on (a) B. subtilis, (b) S. aureus, (c) S. mutans, (d) E. coli, (e) K. oxytoca , and (f) P. aeruginosa by ZnO and Z–Z nanoparticles.
    Figure Legend Snippet: Inhibition zone (mm) formed on (a) B. subtilis, (b) S. aureus, (c) S. mutans, (d) E. coli, (e) K. oxytoca , and (f) P. aeruginosa by ZnO and Z–Z nanoparticles.

    Techniques Used: Inhibition

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    ATCC klebsiella oxytoca
    ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived <t>Klebsiella</t> bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. <t>oxytoca/michiganensis</t> . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .
    Klebsiella Oxytoca, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klebsiella oxytoca/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    klebsiella oxytoca - by Bioz Stars, 2022-12
    94/100 stars
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    ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .

    Journal: Microbial Genomics

    Article Title: Characterisation of the symbionts in the Mediterranean fruit fly gut

    doi: 10.1099/mgen.0.000801

    Figure Lengend Snippet: ANI hierarchical clustering showing the evolutionary relationship of environmentally-derived and host-derived Klebsiella bacteria. The tree was created using the ANI calculator [ 75 ], with Pseudomonas aeruginosa strain JB2 selected as the outgroup. Bacteria derived from animal hosts (red and black), and environmentally derived bacteria (blue), generally fell into three clades: 1) K. pneumoniae , 2) K. variicola and 3) K. oxytoca/michiganensis . With one exception in each group (YH43 and HKUOPLA), all K. pneumoniae are host derived and all K. variicola are environmentally derived. K. oxytoca/K. michiganensis have been isolated from both environmental and animal sources, but their sequences did not cluster according to source status. According to the ANI species threshold set by Kim et al. [ 105 ], Medkleb is conspecific with twelve strains in the ‘Medkleb group’ which have been classified on the RefSeq database as both K. oxytoca and K. michiganensis . However, Kleborate [ 77 ] identified all strains in the Medkleb group (bounded within a blue box) as K. michiganensis, the single strain bounded in a green box as K. grimontii and the two strains bounded in an orange box as K. oxytoca .

    Article Snippet: The 16S rRNA gene sequence of K. oxytoca strain ATCC 13182 (NR_118853.1) was used to locate homologous sequences in the Medkleb genome using the BLASTn algorithm [ ].

    Techniques: Derivative Assay, Isolation