Journal: Drug Design, Development and Therapy

Article Title: 10-Gingerol Enhances the Effect of Taxol in Triple-Negative Breast Cancer via Targeting ADRB2 Signaling

doi: 10.2147/DDDT.S390602

Figure Lengend Snippet: The shRNA Sequence of Lentiviruses

Article Snippet: The following antibodies were used: Bcl-2 (ABCAM, Cambridge, UK, ab182858), Bax (Cell Signaling Technology, Danvers, MA, 2772); cleaved-PARP (Cell Signaling Technology, Danvers, MA, 5625), ADRB2 (Proteintech, Wuhan, China, 13096-1-AP), ERK (ABCAM, Cambridge, UK, ab17942), phospho-ERK1/2 (ABCAM, Cambridge, UK, ab201015), Ki67 (ABCAM, Cambridge, UK, ab16667) and β-actin (Proteintech, Wuhan, China, 66009-1-lg), horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Proteintech, Wuhan, China, SA00001-2), horseradish peroxidase (HRP)-conjugated goat anti-mouse (Proteintech, Wuhan, China, SA00001-1).

Techniques: shRNA, Sequencing

Journal: Drug Design, Development and Therapy

Article Title: 10-Gingerol Enhances the Effect of Taxol in Triple-Negative Breast Cancer via Targeting ADRB2 Signaling

doi: 10.2147/DDDT.S390602

Figure Lengend Snippet: ADRB2 protein is a key target of 10-G action. ( A ) Ligand interaction and binding diagrams of 10-G at ADRB2 active site. ( B ) MDA-MB-231 and SUM-159 cells were transfected with either NC or ADRB2 lentivirus-shRNA for 48 h, and then were treated with different concentrations of 10-G or paclitaxel in combination for 48 h. The cell viability was determined using CCK-8 assay. ( C ) Targeting of ADRB2 with ADRB2 lentivirus-shRNA transfection inhibited the colony formation ability of TNBC cells MDA-MB-231 and SUM-159, and attenuated the cytotoxicity of 10-G induced TNBC cells. Data are represented as the mean value ± SD. *P<0.05, **P<0.01, compared with NC-shRNA.

Article Snippet: The following antibodies were used: Bcl-2 (ABCAM, Cambridge, UK, ab182858), Bax (Cell Signaling Technology, Danvers, MA, 2772); cleaved-PARP (Cell Signaling Technology, Danvers, MA, 5625), ADRB2 (Proteintech, Wuhan, China, 13096-1-AP), ERK (ABCAM, Cambridge, UK, ab17942), phospho-ERK1/2 (ABCAM, Cambridge, UK, ab201015), Ki67 (ABCAM, Cambridge, UK, ab16667) and β-actin (Proteintech, Wuhan, China, 66009-1-lg), horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Proteintech, Wuhan, China, SA00001-2), horseradish peroxidase (HRP)-conjugated goat anti-mouse (Proteintech, Wuhan, China, SA00001-1).

Techniques: Binding Assay, Transfection, shRNA, CCK-8 Assay

Journal: Drug Design, Development and Therapy

Article Title: 10-Gingerol Enhances the Effect of Taxol in Triple-Negative Breast Cancer via Targeting ADRB2 Signaling

doi: 10.2147/DDDT.S390602

Figure Lengend Snippet: 10-G interacts with paclitaxel to inhibit ADRB2/ERK signal. ( A and B ) The protein levels of total ERK and phosphorylated ERK after ADRB2 downregulated by lentivirus-shRNA. ( C and D ) MDA-MB-231 and SUM-159 were treated for 24h with 10-G and paclitaxel alone or in combination, and the protein expression of ADRB2, total ERK and phosphorylated ERK were examined by Western blot. Data are represented as the mean value ± SD. &P<0.05, &&P<0.01, compared with NC-shRNA; **P<0.01, compared with control; +P<0.05, ++P<0.01, compared with 10-G (50μM); ##P<0.01, compared with Paclitaxel; ^P<0.05, ^^P<0.01, compared with Paclitaxel + 10-G (50μM).

Article Snippet: The following antibodies were used: Bcl-2 (ABCAM, Cambridge, UK, ab182858), Bax (Cell Signaling Technology, Danvers, MA, 2772); cleaved-PARP (Cell Signaling Technology, Danvers, MA, 5625), ADRB2 (Proteintech, Wuhan, China, 13096-1-AP), ERK (ABCAM, Cambridge, UK, ab17942), phospho-ERK1/2 (ABCAM, Cambridge, UK, ab201015), Ki67 (ABCAM, Cambridge, UK, ab16667) and β-actin (Proteintech, Wuhan, China, 66009-1-lg), horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Proteintech, Wuhan, China, SA00001-2), horseradish peroxidase (HRP)-conjugated goat anti-mouse (Proteintech, Wuhan, China, SA00001-1).

Techniques: shRNA, Expressing, Western Blot

Journal: Drug Design, Development and Therapy

Article Title: 10-Gingerol Enhances the Effect of Taxol in Triple-Negative Breast Cancer via Targeting ADRB2 Signaling

doi: 10.2147/DDDT.S390602

Figure Lengend Snippet: 10-G enhanced the anti-tumour effect of paclitaxel in vivo. ( A ) Representative tumor image in each treatment group (n=5). ( B ) Tumor volume and tumor weight was measured every 3 days (n=5). ( C ) Representative tumor image of DAPI/TUNEL double-labeling assay of apoptotic cells in each treatment group (n=5). ( D ) The protein levels including Ki67, ADRB2, phosphorylated ERK, Bcl-2, Bax and cleaved PARP was assessed by immunohistochemistry (n=5). Data are represented as the mean value ± SD. **P<0.01, compared with vehicle; ##P<0.01, compared with Paclitaxel. (magnification, ×100).

Article Snippet: The following antibodies were used: Bcl-2 (ABCAM, Cambridge, UK, ab182858), Bax (Cell Signaling Technology, Danvers, MA, 2772); cleaved-PARP (Cell Signaling Technology, Danvers, MA, 5625), ADRB2 (Proteintech, Wuhan, China, 13096-1-AP), ERK (ABCAM, Cambridge, UK, ab17942), phospho-ERK1/2 (ABCAM, Cambridge, UK, ab201015), Ki67 (ABCAM, Cambridge, UK, ab16667) and β-actin (Proteintech, Wuhan, China, 66009-1-lg), horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Proteintech, Wuhan, China, SA00001-2), horseradish peroxidase (HRP)-conjugated goat anti-mouse (Proteintech, Wuhan, China, SA00001-1).

Techniques: In Vivo, TUNEL Assay, Labeling, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 Cells

doi: 10.3390/ijms21217968

Figure Lengend Snippet: Influence of β-adrenoceptor agonist, isoprenaline (ISO, 0.1 and 10 µM), alone or in combination with β-adrenoceptor antagonists, ( a , c ) propranolol (1 µM) or ( b , d ) ICI 118,551 (1 µM) on cell viability of ( a , b ) MCF-10A and ( c , d ) MCF-7 cells, using the MTT assay. Propranolol and ICI 118,551, per se , did not change cell viability in both cell lines tested (see text). Cells were treated with the indicated drugs for 24 h. Results are expressed as percentage of control (solvent) and are presented as mean ± SD. Values are means ± SD from 4–5 (MCF-10A cells) or 5 (MCF-7 cells) independent experiments, as shown. Significant differences from control group: * p ≤ 0.05; one-way analysis of variance (ANOVA), followed by post-hoc multi-comparisons Dunnett’s test. Significant differences from isoprenaline treatment: * p ≤ 0.05; (Student’s t -test).

Article Snippet: Rabbit polyclonal anti-β 2 -adrenoceptor (13096-1-AP) was from Proteintech (Rosemont, IL, USA).

Techniques: MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 Cells

doi: 10.3390/ijms21217968

Figure Lengend Snippet: Influence of β-adrenoceptor agonist, 10 µM isoprenaline (ISO), alone or in combination with β-adrenoceptor antagonists, propranolol or ICI 118,551 (1 µM), in MCF-10A and in MCF-7 cells clonogenic ability. Cells were treated with the indicated drugs for seven days. ( a ) Representative images of the colony formation assay in MCF-10A and in MCF-7 cells in the absence or in the presence of isoprenaline (10 µM). ( b ) Results for absorbance (λ = 600 nm: Abs 600 nm), after crystal violet elution in MCF-10A cells. ( c ) Results for absorbance (λ = 600 nm: Abs 600 nm), after crystal violet elution in MCF-7 cells. Results are expressed as a percentage of control (solvent) and are presented as mean ± SD, from 3 independent experiments. Significant differences from control group: * p ≤ 0.05; one-way analysis of variance (ANOVA), followed by post-hoc multi-comparisons Dunnett’s test.

Article Snippet: Rabbit polyclonal anti-β 2 -adrenoceptor (13096-1-AP) was from Proteintech (Rosemont, IL, USA).

Techniques: Colony Assay