Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A The mRNA expression of PLK4 in EC cell lines. B The protein expression of PLK4 in EC cell lines. C The mRNA expression of PLK4 in KIFC1-overexpressed HEC-1A and KIFC1-knockdown Ishikawa cells. D The protein expression of PLK4 in KIFC1-overexpressed HEC-1A and KIFC1-knockdown Ishikawa cells. E The mRNA expressions of PLK4 and KIFC1 in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). F The protein expressions of PLK4 and KIFC1 in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). G The immunofluorescence analysis with γ-tubulin and α-tubulin antibodies in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa) (scale bar: 10 μm). * P < 0.05, ** P < 0.01, # P < 0.05, ## P < 0.01.

Article Snippet: The antibodies anti-KIFC1 (1:200, No. 20790-1-AP, Proteintech), anti-PLK4 (1:200, No. 12952-1-AP, Proteintech), anti-TRIM37 (1:200, No. 13037-1-AP, Proteintech) and anti-Ki-67 (1:200, 27309-1-AP, Proteintech) were used for IHC staining.

Techniques: Expressing, Knockdown, Immunofluorescence

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A CCK8 assay in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). B Colony formation assay in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). C Transwell assay and quantitative analysis of migratory and invasive abilities of PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa) (scale bar: 100 μm). ** P < 0.01, ## P < 0.01.

Article Snippet: The antibodies anti-KIFC1 (1:200, No. 20790-1-AP, Proteintech), anti-PLK4 (1:200, No. 12952-1-AP, Proteintech), anti-TRIM37 (1:200, No. 13037-1-AP, Proteintech) and anti-Ki-67 (1:200, 27309-1-AP, Proteintech) were used for IHC staining.

Techniques: CCK-8 Assay, Knockdown, Colony Assay, Transwell Assay

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A CHX chase analysis of PLK4 protein half-life after KIFC1 overexpression and knockdown in EC cell lines (HEC-1A and Ishikawa). B The protein expression of KIFC1 and PLK4 in HEC-1A and Ishikawa cells treated with MG132 or CQ after KIFC1 knockdown. C Results of the ubiquitination level of PLK4 in HEK293T cells after KIFC1 overexpression detected by ubiquitination assay. ** P < 0.01, ## P < 0.01, ^^ P < 0.01.

Article Snippet: The antibodies anti-KIFC1 (1:200, No. 20790-1-AP, Proteintech), anti-PLK4 (1:200, No. 12952-1-AP, Proteintech), anti-TRIM37 (1:200, No. 13037-1-AP, Proteintech) and anti-Ki-67 (1:200, 27309-1-AP, Proteintech) were used for IHC staining.

Techniques: Over Expression, Knockdown, Expressing, Ubiquitin Assay

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A The mRNA and protein expressions of TRIM37 in KIFC1-overexpressed HEC-1A and KIFC1-knockdown Ishikawa cells. B The mRNA and protein expressions of TRIM37 in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). C Results of the ubiquitination level of PLK4 in HEK293T cells after KIFC1 or TRIM37 overexpression detected by ubiquitination assay. D The mRNA expressions of KIFC1, TRIM37, and PLK4 in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). E CCK8 assay in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). F Colony formation assay in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). G Transwell assay and quantitative analysis of migratory and invasive abilities of TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa) (scale bar: 100 μm). H The protein expressions of KIFC1, TRIM37, and PLK4 in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). * P < 0.05, ** P < 0.01, # P < 0.05, ## P < 0.01.

Article Snippet: The antibodies anti-KIFC1 (1:200, No. 20790-1-AP, Proteintech), anti-PLK4 (1:200, No. 12952-1-AP, Proteintech), anti-TRIM37 (1:200, No. 13037-1-AP, Proteintech) and anti-Ki-67 (1:200, 27309-1-AP, Proteintech) were used for IHC staining.

Techniques: Knockdown, Over Expression, Ubiquitin Assay, CCK-8 Assay, Colony Assay, Transwell Assay

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A General images of mice and tumors in the subcutaneous xenograft of HEC-1A cells after TRIM37 and KIFC1 overexpression. B The tumor volume and weight. C Representative H & E staining and IHC staining of Ki-67, KIFC1, TRIM37, and PLK4 in tumors (scale bar: 200 μm for left; 100 μm for right). D Representative TUNEL images of tumors (scale bar: 200 μm for left; 100 μm for right). E Representative H & E staining images of lung tissues (scale bar: 200 μm for left; 100 μm for right); F Fluorescence images (left) and quantitative analysis (right) of mouse lung metastasis. * P < 0.05, ** P < 0.01, # P < 0.05, ## P < 0.01.

Article Snippet: The antibodies anti-KIFC1 (1:200, No. 20790-1-AP, Proteintech), anti-PLK4 (1:200, No. 12952-1-AP, Proteintech), anti-TRIM37 (1:200, No. 13037-1-AP, Proteintech) and anti-Ki-67 (1:200, 27309-1-AP, Proteintech) were used for IHC staining.

Techniques: Over Expression, Staining, Immunohistochemistry, TUNEL Assay, Fluorescence

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A The mRNA expression of PLK4 in EC cell lines. B The protein expression of PLK4 in EC cell lines. C The mRNA expression of PLK4 in KIFC1-overexpressed HEC-1A and KIFC1-knockdown Ishikawa cells. D The protein expression of PLK4 in KIFC1-overexpressed HEC-1A and KIFC1-knockdown Ishikawa cells. E The mRNA expressions of PLK4 and KIFC1 in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). F The protein expressions of PLK4 and KIFC1 in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). G The immunofluorescence analysis with γ-tubulin and α-tubulin antibodies in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa) (scale bar: 10 μm). * P < 0.05, ** P < 0.01, # P < 0.05, ## P < 0.01.

Article Snippet: The blots were then blocked in 5% nonfat dry milk solution (Beyotime Biotechnology, Shanghai) at room temperature for 1 h. Subsequently, the blots were incubated with the following primary antibodies: KIFC1 (1:1000, No. 20790-1-AP, Proteintech), γ-tubulin (1:5000, No. 66320-1-Ig, Proteintech), p-H3 (S10) (1:1000, #9701, Cell Signaling Technology), Cyclin A2 (1:1000, #67955, Cell Signaling Technology), Cyclin B1 (1:1000, #4138, Cell Signaling Technology), CDK1 (1:10000, No. 10122-1-AP, Proteintech), CDC2 (1:1000, #77055, Cell Signaling Technology), PLK4 (1:1000, No. 12952-1-AP, Proteintech), TRIM37 (1:15.00, No. 13037-1-AP, Proteintech), Ub (1:1000, ab134953, Abcam), Myc (1:1000, ab32, Abcam), HA (1:1000, ab1424, Abcam), His (1:1000, ab18184, Abcam) and GAPDH (1:10000, ET1601-4, Huabio) at 4 °C overnight.

Techniques: Expressing, Knockdown, Immunofluorescence

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A CCK8 assay in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). B Colony formation assay in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). C Transwell assay and quantitative analysis of migratory and invasive abilities of PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa) (scale bar: 100 μm). ** P < 0.01, ## P < 0.01.

Article Snippet: The blots were then blocked in 5% nonfat dry milk solution (Beyotime Biotechnology, Shanghai) at room temperature for 1 h. Subsequently, the blots were incubated with the following primary antibodies: KIFC1 (1:1000, No. 20790-1-AP, Proteintech), γ-tubulin (1:5000, No. 66320-1-Ig, Proteintech), p-H3 (S10) (1:1000, #9701, Cell Signaling Technology), Cyclin A2 (1:1000, #67955, Cell Signaling Technology), Cyclin B1 (1:1000, #4138, Cell Signaling Technology), CDK1 (1:10000, No. 10122-1-AP, Proteintech), CDC2 (1:1000, #77055, Cell Signaling Technology), PLK4 (1:1000, No. 12952-1-AP, Proteintech), TRIM37 (1:15.00, No. 13037-1-AP, Proteintech), Ub (1:1000, ab134953, Abcam), Myc (1:1000, ab32, Abcam), HA (1:1000, ab1424, Abcam), His (1:1000, ab18184, Abcam) and GAPDH (1:10000, ET1601-4, Huabio) at 4 °C overnight.

Techniques: CCK-8 Assay, Knockdown, Colony Assay, Transwell Assay

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A CHX chase analysis of PLK4 protein half-life after KIFC1 overexpression and knockdown in EC cell lines (HEC-1A and Ishikawa). B The protein expression of KIFC1 and PLK4 in HEC-1A and Ishikawa cells treated with MG132 or CQ after KIFC1 knockdown. C Results of the ubiquitination level of PLK4 in HEK293T cells after KIFC1 overexpression detected by ubiquitination assay. ** P < 0.01, ## P < 0.01, ^^ P < 0.01.

Article Snippet: The blots were then blocked in 5% nonfat dry milk solution (Beyotime Biotechnology, Shanghai) at room temperature for 1 h. Subsequently, the blots were incubated with the following primary antibodies: KIFC1 (1:1000, No. 20790-1-AP, Proteintech), γ-tubulin (1:5000, No. 66320-1-Ig, Proteintech), p-H3 (S10) (1:1000, #9701, Cell Signaling Technology), Cyclin A2 (1:1000, #67955, Cell Signaling Technology), Cyclin B1 (1:1000, #4138, Cell Signaling Technology), CDK1 (1:10000, No. 10122-1-AP, Proteintech), CDC2 (1:1000, #77055, Cell Signaling Technology), PLK4 (1:1000, No. 12952-1-AP, Proteintech), TRIM37 (1:15.00, No. 13037-1-AP, Proteintech), Ub (1:1000, ab134953, Abcam), Myc (1:1000, ab32, Abcam), HA (1:1000, ab1424, Abcam), His (1:1000, ab18184, Abcam) and GAPDH (1:10000, ET1601-4, Huabio) at 4 °C overnight.

Techniques: Over Expression, Knockdown, Expressing, Ubiquitin Assay

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A The mRNA and protein expressions of TRIM37 in KIFC1-overexpressed HEC-1A and KIFC1-knockdown Ishikawa cells. B The mRNA and protein expressions of TRIM37 in PLK4-overexpressed and KIFC1-knockdown EC cell lines (HEC-1A and Ishikawa). C Results of the ubiquitination level of PLK4 in HEK293T cells after KIFC1 or TRIM37 overexpression detected by ubiquitination assay. D The mRNA expressions of KIFC1, TRIM37, and PLK4 in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). E CCK8 assay in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). F Colony formation assay in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). G Transwell assay and quantitative analysis of migratory and invasive abilities of TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa) (scale bar: 100 μm). H The protein expressions of KIFC1, TRIM37, and PLK4 in TRIM37- and KIFC1-overexpressed EC cell lines (HEC-1A and Ishikawa). * P < 0.05, ** P < 0.01, # P < 0.05, ## P < 0.01.

Article Snippet: The blots were then blocked in 5% nonfat dry milk solution (Beyotime Biotechnology, Shanghai) at room temperature for 1 h. Subsequently, the blots were incubated with the following primary antibodies: KIFC1 (1:1000, No. 20790-1-AP, Proteintech), γ-tubulin (1:5000, No. 66320-1-Ig, Proteintech), p-H3 (S10) (1:1000, #9701, Cell Signaling Technology), Cyclin A2 (1:1000, #67955, Cell Signaling Technology), Cyclin B1 (1:1000, #4138, Cell Signaling Technology), CDK1 (1:10000, No. 10122-1-AP, Proteintech), CDC2 (1:1000, #77055, Cell Signaling Technology), PLK4 (1:1000, No. 12952-1-AP, Proteintech), TRIM37 (1:15.00, No. 13037-1-AP, Proteintech), Ub (1:1000, ab134953, Abcam), Myc (1:1000, ab32, Abcam), HA (1:1000, ab1424, Abcam), His (1:1000, ab18184, Abcam) and GAPDH (1:10000, ET1601-4, Huabio) at 4 °C overnight.

Techniques: Knockdown, Over Expression, Ubiquitin Assay, CCK-8 Assay, Colony Assay, Transwell Assay

Journal: Cell Death Discovery

Article Title: KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer

doi: 10.1038/s41420-024-02190-1

Figure Lengend Snippet: A General images of mice and tumors in the subcutaneous xenograft of HEC-1A cells after TRIM37 and KIFC1 overexpression. B The tumor volume and weight. C Representative H & E staining and IHC staining of Ki-67, KIFC1, TRIM37, and PLK4 in tumors (scale bar: 200 μm for left; 100 μm for right). D Representative TUNEL images of tumors (scale bar: 200 μm for left; 100 μm for right). E Representative H & E staining images of lung tissues (scale bar: 200 μm for left; 100 μm for right); F Fluorescence images (left) and quantitative analysis (right) of mouse lung metastasis. * P < 0.05, ** P < 0.01, # P < 0.05, ## P < 0.01.

Article Snippet: The blots were then blocked in 5% nonfat dry milk solution (Beyotime Biotechnology, Shanghai) at room temperature for 1 h. Subsequently, the blots were incubated with the following primary antibodies: KIFC1 (1:1000, No. 20790-1-AP, Proteintech), γ-tubulin (1:5000, No. 66320-1-Ig, Proteintech), p-H3 (S10) (1:1000, #9701, Cell Signaling Technology), Cyclin A2 (1:1000, #67955, Cell Signaling Technology), Cyclin B1 (1:1000, #4138, Cell Signaling Technology), CDK1 (1:10000, No. 10122-1-AP, Proteintech), CDC2 (1:1000, #77055, Cell Signaling Technology), PLK4 (1:1000, No. 12952-1-AP, Proteintech), TRIM37 (1:15.00, No. 13037-1-AP, Proteintech), Ub (1:1000, ab134953, Abcam), Myc (1:1000, ab32, Abcam), HA (1:1000, ab1424, Abcam), His (1:1000, ab18184, Abcam) and GAPDH (1:10000, ET1601-4, Huabio) at 4 °C overnight.

Techniques: Over Expression, Staining, Immunohistochemistry, TUNEL Assay, Fluorescence

Journal: Cell

Article Title: Time-series reconstruction of the molecular architecture of human centriole assembly

doi: 10.1016/j.cell.2024.03.025

Figure Lengend Snippet: Steps of procentriole initiation (A–B′) Centrosomes stained for tubulin and SAS-6 (A and A′) or STIL (B and B′). Arrowheads indicate the presence of SAS-6 (A) and STIL (B) in absence of tubulin signal (A′ and B′), thereafter called “naked.” MC, mature centriole. Scale bars: 200 nm. (C) Percentage of cells presenting a signal for PLK4, SAS-6, STIL, CPAP, CEP135, and CEP44 at the level of the future procentriole in the absence of a tubulin signal. See Table S2 for statistics. (D–K) Procentrioles stained for tubulin and SAS-6 (D and E), STIL (F and G), CPAP (H and I), and CEP135 (J and K). (D), (F), (H), and (J) show naked SAS-6, STIL, CPAP, and CEP135, and (E), (G), (I), and (K) show the protein in early procentrioles. Scale bars: 100 nm. (L and M) Normalized diameter representation of SAS-6 (light blue), STIL (dark blue), CPAP (purple), CEP44 (mauve), and CEP135 (green) at different stages of procentriole assembly according to the tubulin (gray) length (0, <119, <160, and <400 nm). All diameters were normalized on the average tubulin diameter. See Table S2 for detailed statistics. (N) Raw diameters of SAS-6, STIL, CPAP, CEP44, and CEP135 at the same stages of procentriole assembly as in (M). See Table S2 for statistics. (O) Model of the structural reorganization of the cartwheel during the bloom phase. Initially, the cartwheel is formed with SAS-6 and STIL, and part of the pinhead and D2 density (CPAP). A-microtubules appear with the triplet base (CEP135). The microtubule blades separate from each other, reorganizing the pinhead and triplet base until the procentriole is completely formed before the end of the bloom phase. See also Figure S5 .

Article Snippet: Rabbit polyclonal anti-PLK4 , Proteintech , Cat# 28750-1-AP, RRID: AB_2881206.

Techniques: Staining

Journal: Cell

Article Title: Time-series reconstruction of the molecular architecture of human centriole assembly

doi: 10.1016/j.cell.2024.03.025

Figure Lengend Snippet: Methodology of the naked cartwheel observation, related to Figure 3 (A) Overexposed image presented in Figures 3 A and 3B showing the presence of STIL (left, white arrowhead) and SAS-6 (right, white arrowhead) in absence of tubulin signal. Scale bars: 200 nm. (B and C) Procentrioles stained for tubulin (magenta) and PLK4 (green). Note that (B) shows the presence of PLK4 at the level of the future centriole without the tubulin signal (naked), and (C) shows the early procentriole where the PLK4 signal can be found at the level of a tubulin-positive procentriole. Scale bars: 100 nm. (D) PLK4 diameter evolution during centriole assembly (relative to tubulin length), showing no increase in PLK4 diameter. (E and F) Centrioles from cryo-fixed U2OS cells stained for tubulin and SAS-6. (E) shows the presence of SAS-6 at the level of the future centriole without the tubulin signal, and (C) shows the early procentriole where SAS-6 signal is found at the level of a tubulin-positive procentriole. Scale bars: 200 nm. (G) Examples of widefield images used for the quantification of the percentage of cell with naked proteins ( Figure 3 C). Top panel shows a graphical representation of each situation (“not duplicating,” “duplicating—naked,” and “duplicating not naked”). Middle and bottom panels show representative images of each situation. Dashed squares indicate overexposed zoom in, showing the absence of tubulin in the naked situation. Scale bars: 250 nm.

Article Snippet: Rabbit polyclonal anti-PLK4 , Proteintech , Cat# 28750-1-AP, RRID: AB_2881206.

Techniques: Staining

Journal: Cell

Article Title: Time-series reconstruction of the molecular architecture of human centriole assembly

doi: 10.1016/j.cell.2024.03.025

Figure Lengend Snippet: Procentriole elongation (A and B) Centrioles during assembly and at mature stage stained for tubulin (A) and (B) and SAS-6 (A) or γ-tubulin (B). Scale bars: 200 nm. (C) “Average” procentrioles (180–200 nm in length) stained for tubulin and γ-tubulin, PLK4, CEP135, CEP44, SAS-6, STIL, and CPAP. Asterisks indicate additional distal localization of CPAP and CEP135. (D) Relative protein longitudinal and radial positions compared with tubulin. See Table S2 for statistics. (E) Structural model of the procentriole, lateral view. (F–J) Evolution of SAS-6 (F), STIL (G), CEP44 (H), CPAP (I), and CEP135 (J) length over tubulin growth during procentriole assembly. CPAP and CEP135 present a dual localization in the proximal (CPAP purple and CEP135 dark red) and distal (CPAP red and CEP135 green) regions. This figure focuses on the proximal localization (see Figure 6 for distal analysis). The evolution of each set of data was represented as loess curves (black line) to estimate the behavior of each protein during their growth (relative to tubulin). (K) Merged data from graphs (F) to (J) with the dot plots and the loess curves for each protein, showing three main clusters based on their similar behaviors (SAS-6/STIL, CPAP/CEP44, and CEP135). The breakpoints, when the growth rates change significantly, are indicated in Figure S6 . (L) Protein growth rate relative to tubulin growth before (plain bars) and after (striped bars) breakpoint. See Table S2 for statistics. (M) Model of the formation and elongation of the cartwheel and procentriole during the bloom phase. A cartwheel layer assembles before γ-TuRC recruitment and microtubule blade assembly. The microtubules elongate approximately two times faster than the cartwheel structures, the pinhead, the D2 density, and the triplet base. During assembly, a cartwheel layer is always lower than the microtubules. After about 100 nm of cartwheel elongation, it stops growing, in contrast to the pinhead and D2 density, which grows to about 190 nm. See also Figure S6 .

Article Snippet: Rabbit polyclonal anti-PLK4 , Proteintech , Cat# 28750-1-AP, RRID: AB_2881206.

Techniques: Staining

Journal: Cell

Article Title: Time-series reconstruction of the molecular architecture of human centriole assembly

doi: 10.1016/j.cell.2024.03.025

Figure Lengend Snippet: Growth rate of the cartwheel and pinhead components, related to Figure 4 (A) Distance between protein signal and tubulin signal start (sticking out). Average ± SD are as follows: PLK4 = −30.59 ± 12.5 nm, γ-tub = −28.25 ± 11.2 nm, SAS-6 = −16.72 ± 8.2 nm, STIL = −17.72 ± 11.3 nm, CPAP = −13.93 ± 16.6, CEP135 = −0.37 ± 13.7 nm, and CEP44 = 2.36 ± 11.6 nm. n = 38, 28, 79, 81, 58, 57, and 41 centrioles for γ-tub, PLK4, SAS-6, STIL, CPAP, CEP135, and CEP44, respectively, from three independent experiments. Statistical differences were assessed using a one-way ANOVA followed by Tukey’s post hoc test. ∗∗∗∗ p < 0.0001 in all conditions tested. (B–F) Plots of loess fits and superimposed segmented regressions per protein showing that the segmented regressions are a good representation of the overall data (as in Figure S4 D).

Article Snippet: Rabbit polyclonal anti-PLK4 , Proteintech , Cat# 28750-1-AP, RRID: AB_2881206.

Techniques:

Journal: Cell

Article Title: Time-series reconstruction of the molecular architecture of human centriole assembly

doi: 10.1016/j.cell.2024.03.025

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-PLK4 , Proteintech , Cat# 28750-1-AP, RRID: AB_2881206.

Techniques: Recombinant, Software