regular powerlyzer powersoil kit  (Qiagen)


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    Name:
    DNeasy PowerLyzer PowerSoil Kit
    Description:
    For the bead based isolation of DNA from tough soil microbes Kit contents For the isolation of DNA from tough soil microbes optimized for use with bead based homogenizers Benefits Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid Ready to use high quality DNA for downstream applications Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minu
    Catalog Number:
    12855-100
    Price:
    550
    Category:
    DNeasy PowerLyzer PowerSoil Kit
    Buy from Supplier


    Structured Review

    Qiagen regular powerlyzer powersoil kit
    DNeasy PowerLyzer PowerSoil Kit
    For the bead based isolation of DNA from tough soil microbes Kit contents For the isolation of DNA from tough soil microbes optimized for use with bead based homogenizers Benefits Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid Ready to use high quality DNA for downstream applications Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minu
    https://www.bioz.com/result/regular powerlyzer powersoil kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    regular powerlyzer powersoil kit - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples"

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-24082-y

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.
    Figure Legend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Techniques Used: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.
    Figure Legend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Techniques Used: Radioactivity, DNA Extraction

    2) Product Images from "DNA extraction approaches substantially influence the assessment of the human breast milk microbiome"

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-55568-y

    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Figure Legend Snippet: nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Techniques Used: DNA Extraction

    Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Figure Legend Snippet: Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Techniques Used: DNA Extraction

    3) Product Images from "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples"

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-24082-y

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.
    Figure Legend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Techniques Used: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.
    Figure Legend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Techniques Used: Radioactivity, DNA Extraction

    4) Product Images from "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples"

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-24082-y

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.
    Figure Legend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Techniques Used: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.
    Figure Legend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Techniques Used: Radioactivity, DNA Extraction

    5) Product Images from "Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?"

    Article Title: Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0232215

    Taxa plots summarizing the relative abundances of bacterial genera identified in the respiratory samples of 2 patients (P1 and P2) using 16S targeted amplicon sequencing. DNA was extracted using 2 protocols: the automated QIAsymphony extraction [AQE] with DSP DNA midi kit, and the manual PowerSoil® MoBio extraction [MPE]; and amplified by ultra-deep sequencing (MiSeq®, Illumina) using two 16S targets (V1-V2 and V3-V4).
    Figure Legend Snippet: Taxa plots summarizing the relative abundances of bacterial genera identified in the respiratory samples of 2 patients (P1 and P2) using 16S targeted amplicon sequencing. DNA was extracted using 2 protocols: the automated QIAsymphony extraction [AQE] with DSP DNA midi kit, and the manual PowerSoil® MoBio extraction [MPE]; and amplified by ultra-deep sequencing (MiSeq®, Illumina) using two 16S targets (V1-V2 and V3-V4).

    Techniques Used: Amplification, Sequencing

    Workflow of the study. A- Comparison of two DNA extraction protocols (automated QIAsymphony extraction [AQE] using DSP DNA midi kit and manual PowerSoil® MoBio extraction [MPE]) in terms of quantity and quality of DNA extracted from 6 sputa (6 patients, P1, P2, P3, P4, P5, P6). B- Comparison of bacterial and fungal diversity detected in two sputa (2 patients, P1, P2) spiked with 10 5 conidia of Aspergillus section Fumigati and Aspergillus section Nigri using (i) culture method and (ii) 16S/ITS targeted amplicon sequencing (DNA extracted using AQE or MPE protocols; amplification targets V1-V2 and V3-V4 16S for bacteria, and ITS2 and ITS2 for fungi; MiSeq® Illumina, 2x300 v3 kit).
    Figure Legend Snippet: Workflow of the study. A- Comparison of two DNA extraction protocols (automated QIAsymphony extraction [AQE] using DSP DNA midi kit and manual PowerSoil® MoBio extraction [MPE]) in terms of quantity and quality of DNA extracted from 6 sputa (6 patients, P1, P2, P3, P4, P5, P6). B- Comparison of bacterial and fungal diversity detected in two sputa (2 patients, P1, P2) spiked with 10 5 conidia of Aspergillus section Fumigati and Aspergillus section Nigri using (i) culture method and (ii) 16S/ITS targeted amplicon sequencing (DNA extracted using AQE or MPE protocols; amplification targets V1-V2 and V3-V4 16S for bacteria, and ITS2 and ITS2 for fungi; MiSeq® Illumina, 2x300 v3 kit).

    Techniques Used: DNA Extraction, Amplification, Sequencing

    Taxa plots summarizing the relative abundances of fungal genera or sections identified in the respiratory samples of 2 patients (P1 and P2) using culture and ITS targeted amplicon sequencing. The sputa of P1 and P2 were spiked with 10 5 CFU/ml of Aspergillus section Nigri and Aspergillus section Fumigati . For ITS targeted amplicon sequencing analysis, DNA was extracted from the respiratory samples using 2 protocols: the automated QIAsymphony extraction [AQE] with DSP DNA midi kit, and the manual PowerSoil® MoBio extraction [MPE]; and amplified by ultra-deep sequencing (MiSeq®, Illumina) using two ITS targets (ITS1 and ITS2). Methods used the same starting quantities of samples and the final volumes were equal. Metagenomic analysis revealed 5 extremely low-represented genera (overall relative abundance
    Figure Legend Snippet: Taxa plots summarizing the relative abundances of fungal genera or sections identified in the respiratory samples of 2 patients (P1 and P2) using culture and ITS targeted amplicon sequencing. The sputa of P1 and P2 were spiked with 10 5 CFU/ml of Aspergillus section Nigri and Aspergillus section Fumigati . For ITS targeted amplicon sequencing analysis, DNA was extracted from the respiratory samples using 2 protocols: the automated QIAsymphony extraction [AQE] with DSP DNA midi kit, and the manual PowerSoil® MoBio extraction [MPE]; and amplified by ultra-deep sequencing (MiSeq®, Illumina) using two ITS targets (ITS1 and ITS2). Methods used the same starting quantities of samples and the final volumes were equal. Metagenomic analysis revealed 5 extremely low-represented genera (overall relative abundance

    Techniques Used: Amplification, Sequencing

    6) Product Images from "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples"

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-24082-y

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.
    Figure Legend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Techniques Used: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.
    Figure Legend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Techniques Used: Radioactivity, DNA Extraction

    Related Articles

    Modification:

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples
    Article Snippet: DNA loss during extraction protocol and quality control of G2 beads Escherichia coli was grown to near late log phase, and 100,000 dpm 3 H-thymidine (Sigma-Aldrich, Copenhagen, Denmark) was added in late log phase, while the culture was allowed to continue growing and incorporating the 3 H-thymidine (the late addition maximizes the amount of 3 H that is incorporated into DNA). .. Twenty-five µl of the culture was added to 250 mg of soil and immediately three replicate DNA extractions were performed using the regular PowerLyzer PowerSoil kit (MOBIO, Carlsbad, CA, USA), and three using G2 modified bead tubes (Ampliqon, Odense, Denmark) but otherwise following the exact same protocol. .. One aliquot of the 3 H labeled culture and one 10% (vol/vol) aliquot of the DNA extraction were withdrawn at four steps in the protocol (MOBIO): (1) after step 7 (lysis); (2) after step 11 (first inhibitor removal precipitation); (3) after step 14 (second inhibitor removal precipitation); and, finally, (4) after step 23 (final step).

    DNA Extraction:

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples
    Article Snippet: HiSeq sequencing of potential DNA contamination from G2 Cupriavidus pinatubonensis JMP134 was inoculated in Luria–Bertania broth (Alpha BioScience, Baltimore, MD, USA) and incubated with shaking for 24 hours at 28 °C. .. DNA was extracted from the 2 ml culture both with and without the addition of the G2 compound using the PowerLyzer PowerSoil DNA Isolation Kit (MOBIO). .. A single-end Illumina HiSeq sequencing library was prepared using a modified version of the NEBNext ® DNA library Prep Master Mix Set kit (New England BioLabs, MA, USA).

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
    Article Snippet: .. DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method . ..

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples
    Article Snippet: .. DNA was extracted from the 0.25 g soil samples by participating laboratories using the protocol provided with the PowerLyzer PowerSoil DNA extraction kit (MOBIO, Carlsbad, CA, USA). ..

    Article Title: Functional and Structural Succession of Soil Microbial Communities below Decomposing Human Cadavers
    Article Snippet: .. DNA was extracted using the PowerLyzer PowerSoil DNA Isolation Kit (MOBIO Laboratories, Inc. Carlsbad, CA.) and PowerLyzer instrument (MOBIO), with an added heated incubation step to increase yield: samples were incubated at 65°C for 10 minutes in a water bath and at 95°C in a heat block for another 10 minutes prior to bead-beating. .. DNA was sent to the Genomic Services Lab at Hudson Alpha Institute for Biotechnology (Huntsville, AL) for library preparation using universal bacterial primers 515F and 806R [ ] to amplify the V4 region of bacterial 16S rDNA, and sequencing on the Illumina MiSeq platform to generate 150 bp paired-end reads.

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples
    Article Snippet: .. DNA extraction protocol DNA was extracted from the 0.25 g soil samples by participating laboratories using the protocol provided with the PowerLyzer PowerSoil DNA extraction kit (MOBIO, Carlsbad, CA, USA). ..

    Article Title: Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?
    Article Snippet: .. Manual Powersoil® extraction (MPE), MoBio PowerLyzer PowerSoil® DNA Isolation kit (QIAGEN, Carlsbad, USA) The aliquots were pipetted into PowerLyzer® 0.1 mm Glass Bead Tubes and submitted to mechanical lysis: two 60-sec cycles at 6400 rpm on MagNA Lyser Instrument (Roche, Mannheim, Germany) and 1 min rest on ice between cycles. .. We then proceeded as per manufacturer’s instructions with minor adjustments: centrifugation of the Glass Bead Tubes for 5 mins instead of 30 secs (step 7 of the protocol, after mechanical lysis) and 5 mins waiting at room temperature (instead of 30 secs) before spinning at 10000g for 1 min (step 22) [ ].

    Article Title: Architectural Design Drives the Biogeography of Indoor Bacterial Communities
    Article Snippet: .. Whole genomic DNA was isolated from samples using MO BIO PowerLyzer™ PowerSoil® DNA Isolation Kit (MO BIO, Carlsbad, CA) according to manufacturer’s instructions with the following modifications: bead tubes were vortexed for 10 min; solutions C4 and C5 were substituted for PW3 and PW4/PW5 solutions from the same manufacturer’s PowerWater® DNA isolation kit. .. Bacterial communities were profiled by sequencing a ∼420 bp fragment of the V4 region of the bacterial 16S rRNA gene using a custom library preparation protocol .

    Incubation:

    Article Title: Functional and Structural Succession of Soil Microbial Communities below Decomposing Human Cadavers
    Article Snippet: .. DNA was extracted using the PowerLyzer PowerSoil DNA Isolation Kit (MOBIO Laboratories, Inc. Carlsbad, CA.) and PowerLyzer instrument (MOBIO), with an added heated incubation step to increase yield: samples were incubated at 65°C for 10 minutes in a water bath and at 95°C in a heat block for another 10 minutes prior to bead-beating. .. DNA was sent to the Genomic Services Lab at Hudson Alpha Institute for Biotechnology (Huntsville, AL) for library preparation using universal bacterial primers 515F and 806R [ ] to amplify the V4 region of bacterial 16S rDNA, and sequencing on the Illumina MiSeq platform to generate 150 bp paired-end reads.

    Blocking Assay:

    Article Title: Functional and Structural Succession of Soil Microbial Communities below Decomposing Human Cadavers
    Article Snippet: .. DNA was extracted using the PowerLyzer PowerSoil DNA Isolation Kit (MOBIO Laboratories, Inc. Carlsbad, CA.) and PowerLyzer instrument (MOBIO), with an added heated incubation step to increase yield: samples were incubated at 65°C for 10 minutes in a water bath and at 95°C in a heat block for another 10 minutes prior to bead-beating. .. DNA was sent to the Genomic Services Lab at Hudson Alpha Institute for Biotechnology (Huntsville, AL) for library preparation using universal bacterial primers 515F and 806R [ ] to amplify the V4 region of bacterial 16S rDNA, and sequencing on the Illumina MiSeq platform to generate 150 bp paired-end reads.

    Lysis:

    Article Title: Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter?
    Article Snippet: .. Manual Powersoil® extraction (MPE), MoBio PowerLyzer PowerSoil® DNA Isolation kit (QIAGEN, Carlsbad, USA) The aliquots were pipetted into PowerLyzer® 0.1 mm Glass Bead Tubes and submitted to mechanical lysis: two 60-sec cycles at 6400 rpm on MagNA Lyser Instrument (Roche, Mannheim, Germany) and 1 min rest on ice between cycles. .. We then proceeded as per manufacturer’s instructions with minor adjustments: centrifugation of the Glass Bead Tubes for 5 mins instead of 30 secs (step 7 of the protocol, after mechanical lysis) and 5 mins waiting at room temperature (instead of 30 secs) before spinning at 10000g for 1 min (step 22) [ ].

    Isolation:

    Article Title: Architectural Design Drives the Biogeography of Indoor Bacterial Communities
    Article Snippet: .. Whole genomic DNA was isolated from samples using MO BIO PowerLyzer™ PowerSoil® DNA Isolation Kit (MO BIO, Carlsbad, CA) according to manufacturer’s instructions with the following modifications: bead tubes were vortexed for 10 min; solutions C4 and C5 were substituted for PW3 and PW4/PW5 solutions from the same manufacturer’s PowerWater® DNA isolation kit. .. Bacterial communities were profiled by sequencing a ∼420 bp fragment of the V4 region of the bacterial 16S rRNA gene using a custom library preparation protocol .

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  • 99
    Qiagen regular powerlyzer powersoil kit
    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO <t>PowerLyzer</t> <t>PowerSoil</t> DNA extraction kit with or without G2 coated onto the beads.
    Regular Powerlyzer Powersoil Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/regular powerlyzer powersoil kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    regular powerlyzer powersoil kit - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Journal: Scientific Reports

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    doi: 10.1038/s41598-018-24082-y

    Figure Lengend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Article Snippet: Twenty-five µl of the culture was added to 250 mg of soil and immediately three replicate DNA extractions were performed using the regular PowerLyzer PowerSoil kit (MOBIO, Carlsbad, CA, USA), and three using G2 modified bead tubes (Ampliqon, Odense, Denmark) but otherwise following the exact same protocol.

    Techniques: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Journal: Scientific Reports

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    doi: 10.1038/s41598-018-24082-y

    Figure Lengend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Article Snippet: Twenty-five µl of the culture was added to 250 mg of soil and immediately three replicate DNA extractions were performed using the regular PowerLyzer PowerSoil kit (MOBIO, Carlsbad, CA, USA), and three using G2 modified bead tubes (Ampliqon, Odense, Denmark) but otherwise following the exact same protocol.

    Techniques: Radioactivity, DNA Extraction

    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Journal: Scientific Reports

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    doi: 10.1038/s41598-019-55568-y

    Figure Lengend Snippet: nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Article Snippet: DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method .

    Techniques: DNA Extraction

    Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Journal: Scientific Reports

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    doi: 10.1038/s41598-019-55568-y

    Figure Lengend Snippet: Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Article Snippet: DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method .

    Techniques: DNA Extraction

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Journal: Scientific Reports

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    doi: 10.1038/s41598-018-24082-y

    Figure Lengend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Article Snippet: DNA was extracted from the 0.25 g soil samples by participating laboratories using the protocol provided with the PowerLyzer PowerSoil DNA extraction kit (MOBIO, Carlsbad, CA, USA).

    Techniques: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Journal: Scientific Reports

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    doi: 10.1038/s41598-018-24082-y

    Figure Lengend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Article Snippet: DNA was extracted from the 0.25 g soil samples by participating laboratories using the protocol provided with the PowerLyzer PowerSoil DNA extraction kit (MOBIO, Carlsbad, CA, USA).

    Techniques: Radioactivity, DNA Extraction