regular powerlyzer powersoil kit  (Qiagen)


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    Name:
    DNeasy PowerLyzer PowerSoil Kit
    Description:
    For the bead based isolation of DNA from tough soil microbes Kit contents For the isolation of DNA from tough soil microbes optimized for use with bead based homogenizers Benefits Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid Ready to use high quality DNA for downstream applications Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minu
    Catalog Number:
    12855-100
    Price:
    550
    Category:
    DNeasy PowerLyzer PowerSoil Kit
    Buy from Supplier


    Structured Review

    Qiagen regular powerlyzer powersoil kit
    DNeasy PowerLyzer PowerSoil Kit
    For the bead based isolation of DNA from tough soil microbes Kit contents For the isolation of DNA from tough soil microbes optimized for use with bead based homogenizers Benefits Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid Ready to use high quality DNA for downstream applications Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minu
    https://www.bioz.com/result/regular powerlyzer powersoil kit/product/Qiagen
    Average 99 stars, based on 815 article reviews
    Price from $9.99 to $1999.99
    regular powerlyzer powersoil kit - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples"

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-24082-y

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.
    Figure Legend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Techniques Used: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.
    Figure Legend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Techniques Used: Radioactivity, DNA Extraction

    2) Product Images from "Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples"

    Article Title: Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-24082-y

    Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.
    Figure Legend Snippet: Log copy number of 16S rRNA genes found in one clay subsoil tested in 11 different laboratories using MOBIO PowerLyzer PowerSoil DNA extraction kit with or without G2 coated onto the beads.

    Techniques Used: DNA Extraction

    Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.
    Figure Legend Snippet: Radioactivity measured at different steps in the MOBIO PowerLyzer PowerSoil DNA extraction procedure. Radioactivity was measured after step 7: supernatant of bead-beated soil, 11: supernatant after removing non-DNA organic and inorganic material including humic substances, cell debris and proteins, 14: supernatant after second removal of non-DNA organic and inorganic material, and 23: final DNA elution.

    Techniques Used: Radioactivity, DNA Extraction

    3) Product Images from "DNA extraction approaches substantially influence the assessment of the human breast milk microbiome"

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-55568-y

    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Figure Legend Snippet: nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Techniques Used: DNA Extraction

    Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Figure Legend Snippet: Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Techniques Used: DNA Extraction

    Related Articles

    DNA Extraction:

    Article Title: Diverse deep-sea anglerfishes share a genetically reduced luminous symbiont that is acquired from the environment
    Article Snippet: .. DNA extraction from samples collected in the Gulf of Mexico was performed at the Marine Microbiology and Genetics Laboratory at Nova Southeastern University’s Halmos College of Natural Sciences and Oceanography using the PowerLyzer PowerSoil kit (MoBio) as is described in Samples collected in the Northern Atlantic were extracted using the DNeasy Blood and Tissue Kit (Qiagen). .. Paired-end 250 base pair Illumina sequence libraries were prepared using the Nextera kit (Illumina, San Diego, CA) and sequenced using HiSeq2500 at the Cornell University Institute of Biotechnology Resource Center Genomics Facility.

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: .. 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. PCR amplification was performed on the 16S rRNA gene V4 hypervariable region using the primers 16SV4_515F (GTGYCAGCMGCCGCGGTA) and 16SV4_806R (GGACTACHVGGGTWTCTAAT), with a 12-bp unique Golay barcoding [ , ].

    Article Title: Oleoylethanolamide treatment affects gut microbiota composition and the expression of intestinal cytokines in Peyer’s patches of mice
    Article Snippet: .. The bacterial genomic DNA extraction was carried out with DNeasy PowerLyzer PowerSoil Kit (Qiagen, Hilden, Germania) following the manufacturer’s instructions. ..

    Article Title: Effect of Cell Concentration on the Persistence in the Human Intestine of Four Probiotic Strains Administered through a Multispecies Formulation
    Article Snippet: Paragraph title: 2.2. DNA Extraction from Fecal Samples ... After homogenization, 250 mg of feces were weighed and processed using a DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany) for the isolation of total DNA with a minor modification that included incubating samples at 65 °C for 10 min after the addition of the power bead and C1 solutions provided with the kit.

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
    Article Snippet: .. DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method . ..

    Article Title: Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile
    Article Snippet: Paragraph title: DNA extraction and sequencing. ... DNA was extracted using the DNeasy PowerLyzer PowerSoil kit (Qiagen; Hilden, Germany) according to the manufacturer’s protocol with a few adjustments, as in Krüger et al. ( ).

    Article Title: Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
    Article Snippet: .. DNA Extraction and Illumina Library Preparation DNA extractions were carried out using the Qiagen DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany). ..

    Article Title: Microbial Community Patterns Associated with Automated Teller Machine Keypads in New York City
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequencing. ... We extracted DNA using a MoBio PowerLyzer PowerSoil extraction kit (catalog no. 12855-100) according to manufacturer instructions.

    Article Title: The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters
    Article Snippet: Genomic DNA was isolated using the standard protocol from the MO BIO PowerSoil DNA Isolation Kit (MO BIO Laboratories). .. Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer.

    Amplification:

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: .. 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. PCR amplification was performed on the 16S rRNA gene V4 hypervariable region using the primers 16SV4_515F (GTGYCAGCMGCCGCGGTA) and 16SV4_806R (GGACTACHVGGGTWTCTAAT), with a 12-bp unique Golay barcoding [ , ].

    Article Title: Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile
    Article Snippet: DNA was extracted using the DNeasy PowerLyzer PowerSoil kit (Qiagen; Hilden, Germany) according to the manufacturer’s protocol with a few adjustments, as in Krüger et al. ( ). .. The DNA was PCR amplified using the primer set 341F (5′-CCTACGGGNGGCWGCAG-3′) and 806R (5′-GACTACHVGGGTATCTAATCC-3′) ( ) targeting the hypervariable V3-V4 regions of bacterial 16S rRNA genes.

    Article Title: Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
    Article Snippet: DNA Extraction and Illumina Library Preparation DNA extractions were carried out using the Qiagen DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany). .. Samples were quantified using a Qubit 2.0 fluorometer (Invitrogen) and diluted to 5ng/ul for PCR amplification and subsequent amplicon sequencing.

    Article Title: Microbial Community Patterns Associated with Automated Teller Machine Keypads in New York City
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequencing. ... We extracted DNA using a MoBio PowerLyzer PowerSoil extraction kit (catalog no. 12855-100) according to manufacturer instructions.

    Article Title: Latent-period stool proteomic assay of multiple sclerosis model indicates protective capacity of host-expressed protease inhibitors
    Article Snippet: .. 16S rRNA microbial community profiling Fecal DNA was isolated (MoBio PowerLyzer PowerSoil Kit) and the V4 region of the bacterial 16S rRNA gene was amplified using barcoded forward primers (515F, 806R) from the Earth Microbiome Project and http://www.earthmicrobiome.org/protocols-and-standards/16S/ . .. Samples were sequenced by paired end 150 base pair reads at the Harvard Medical School Biopolymers Facility using the MiSeq platform (Illumina).

    High Throughput Screening Assay:

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: .. 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. PCR amplification was performed on the 16S rRNA gene V4 hypervariable region using the primers 16SV4_515F (GTGYCAGCMGCCGCGGTA) and 16SV4_806R (GGACTACHVGGGTWTCTAAT), with a 12-bp unique Golay barcoding [ , ].

    Homogenization:

    Article Title: Effect of Cell Concentration on the Persistence in the Human Intestine of Four Probiotic Strains Administered through a Multispecies Formulation
    Article Snippet: .. After homogenization, 250 mg of feces were weighed and processed using a DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany) for the isolation of total DNA with a minor modification that included incubating samples at 65 °C for 10 min after the addition of the power bead and C1 solutions provided with the kit. .. Steps of mechanical bacterial cell disruption were performed using a Precellys bead beater (Advanced Biotech Italia s.r.l., Seveso, Italy) that was kept in a cold room (3 cycles of 6800 rpm × 30 s).

    Article Title: Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
    Article Snippet: DNA Extraction and Illumina Library Preparation DNA extractions were carried out using the Qiagen DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany). .. These were carried out following the manufacturer’s instructions, except for the homogenization step that was done using a FastPrep-24 (45 s at 6 m/s; MP Biomedicals, LLC, Solon, OH).

    Northern Blot:

    Article Title: Diverse deep-sea anglerfishes share a genetically reduced luminous symbiont that is acquired from the environment
    Article Snippet: .. DNA extraction from samples collected in the Gulf of Mexico was performed at the Marine Microbiology and Genetics Laboratory at Nova Southeastern University’s Halmos College of Natural Sciences and Oceanography using the PowerLyzer PowerSoil kit (MoBio) as is described in Samples collected in the Northern Atlantic were extracted using the DNeasy Blood and Tissue Kit (Qiagen). .. Paired-end 250 base pair Illumina sequence libraries were prepared using the Nextera kit (Illumina, San Diego, CA) and sequenced using HiSeq2500 at the Cornell University Institute of Biotechnology Resource Center Genomics Facility.

    Picogreen Assay:

    Article Title: The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters
    Article Snippet: Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer. .. Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer.

    dsDNA Assay:

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. The PCR products were purified using a QIAquick Gel Extraction Kit (QIAGEN) and quantified using a Qubit 2.0 Fluorometic High Sensitivity dsDNA Assay (Life Technologies).

    Purification:

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. The PCR products were purified using a QIAquick Gel Extraction Kit (QIAGEN) and quantified using a Qubit 2.0 Fluorometic High Sensitivity dsDNA Assay (Life Technologies).

    Article Title: Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile
    Article Snippet: DNA was extracted using the DNeasy PowerLyzer PowerSoil kit (Qiagen; Hilden, Germany) according to the manufacturer’s protocol with a few adjustments, as in Krüger et al. ( ). .. The purified PCR products (2 × 300-bp reads) were sequenced on the Illumina MiSeq platform by Macrogen (Seoul, South Korea).

    Sequencing:

    Article Title: Diverse deep-sea anglerfishes share a genetically reduced luminous symbiont that is acquired from the environment
    Article Snippet: Paragraph title: Genome sequencing, assembly, and annotation ... DNA extraction from samples collected in the Gulf of Mexico was performed at the Marine Microbiology and Genetics Laboratory at Nova Southeastern University’s Halmos College of Natural Sciences and Oceanography using the PowerLyzer PowerSoil kit (MoBio) as is described in Samples collected in the Northern Atlantic were extracted using the DNeasy Blood and Tissue Kit (Qiagen).

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: .. 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. PCR amplification was performed on the 16S rRNA gene V4 hypervariable region using the primers 16SV4_515F (GTGYCAGCMGCCGCGGTA) and 16SV4_806R (GGACTACHVGGGTWTCTAAT), with a 12-bp unique Golay barcoding [ , ].

    Article Title: Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile
    Article Snippet: Paragraph title: DNA extraction and sequencing. ... DNA was extracted using the DNeasy PowerLyzer PowerSoil kit (Qiagen; Hilden, Germany) according to the manufacturer’s protocol with a few adjustments, as in Krüger et al. ( ).

    Article Title: Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
    Article Snippet: DNA Extraction and Illumina Library Preparation DNA extractions were carried out using the Qiagen DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany). .. Samples were quantified using a Qubit 2.0 fluorometer (Invitrogen) and diluted to 5ng/ul for PCR amplification and subsequent amplicon sequencing.

    Article Title: Microbial Community Patterns Associated with Automated Teller Machine Keypads in New York City
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequencing. ... We extracted DNA using a MoBio PowerLyzer PowerSoil extraction kit (catalog no. 12855-100) according to manufacturer instructions.

    Article Title: Latent-period stool proteomic assay of multiple sclerosis model indicates protective capacity of host-expressed protease inhibitors
    Article Snippet: 16S rRNA microbial community profiling Fecal DNA was isolated (MoBio PowerLyzer PowerSoil Kit) and the V4 region of the bacterial 16S rRNA gene was amplified using barcoded forward primers (515F, 806R) from the Earth Microbiome Project and http://www.earthmicrobiome.org/protocols-and-standards/16S/ . .. Sequence quality was evaluated with FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ).

    Polymerase Chain Reaction:

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: .. 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. PCR amplification was performed on the 16S rRNA gene V4 hypervariable region using the primers 16SV4_515F (GTGYCAGCMGCCGCGGTA) and 16SV4_806R (GGACTACHVGGGTWTCTAAT), with a 12-bp unique Golay barcoding [ , ].

    Article Title: Bacterial Dispersers along Preferential Flow Paths of a Clay Till Depth Profile
    Article Snippet: DNA was extracted using the DNeasy PowerLyzer PowerSoil kit (Qiagen; Hilden, Germany) according to the manufacturer’s protocol with a few adjustments, as in Krüger et al. ( ). .. The DNA was PCR amplified using the primer set 341F (5′-CCTACGGGNGGCWGCAG-3′) and 806R (5′-GACTACHVGGGTATCTAATCC-3′) ( ) targeting the hypervariable V3-V4 regions of bacterial 16S rRNA genes.

    Article Title: Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
    Article Snippet: DNA Extraction and Illumina Library Preparation DNA extractions were carried out using the Qiagen DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany). .. Samples were quantified using a Qubit 2.0 fluorometer (Invitrogen) and diluted to 5ng/ul for PCR amplification and subsequent amplicon sequencing.

    Article Title: Microbial Community Patterns Associated with Automated Teller Machine Keypads in New York City
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequencing. ... We extracted DNA using a MoBio PowerLyzer PowerSoil extraction kit (catalog no. 12855-100) according to manufacturer instructions.

    Incubation:

    Article Title: The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters
    Article Snippet: Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer. .. The specimens were heated at 65°C for 10 min, incubated at 95°C for 10 min, and processed per PowerSoil instructions.

    Article Title: Genomic Characterization of a Mercury Resistant Arthrobacter sp. H-02-3 Reveals the Presence Heavy Metal and Antibiotic Resistance Determinants
    Article Snippet: Genomic Characterization and Comparative Genomics of Strain H-02-3 To understand the genomic characteristics of strain H-02-3 in reference to Hg cycling, a single colony of the bacterium growing on LB + Hg plate was inoculated into liquid LB media and incubated overnight at 30°C in a shaker at 100 rpm. .. After overnight growth, the media was centrifuged at 7500 rpm for 10 min to obtain a pellet, which was used for DNA extraction with Qiagen’s DNeasy PowerLyzer Kit and sequenced using an Illumina HiSeq2000 instrument ( ).

    other:

    Article Title: Does the endometrial cavity have a molecular microbial signature?
    Article Snippet: Extraction of DNA from samples Genomic DNA was extracted from swab samples using a QIAGEN DNeasy PowerLyzer PowerSoil Kit with minor modifications to the manufacturer’s protocol.

    Isolation:

    Article Title: Effect of Cell Concentration on the Persistence in the Human Intestine of Four Probiotic Strains Administered through a Multispecies Formulation
    Article Snippet: .. After homogenization, 250 mg of feces were weighed and processed using a DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany) for the isolation of total DNA with a minor modification that included incubating samples at 65 °C for 10 min after the addition of the power bead and C1 solutions provided with the kit. .. Steps of mechanical bacterial cell disruption were performed using a Precellys bead beater (Advanced Biotech Italia s.r.l., Seveso, Italy) that was kept in a cold room (3 cycles of 6800 rpm × 30 s).

    Article Title: The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters
    Article Snippet: Genomic DNA was isolated using the standard protocol from the MO BIO PowerSoil DNA Isolation Kit (MO BIO Laboratories). .. Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer.

    Article Title: Latent-period stool proteomic assay of multiple sclerosis model indicates protective capacity of host-expressed protease inhibitors
    Article Snippet: .. 16S rRNA microbial community profiling Fecal DNA was isolated (MoBio PowerLyzer PowerSoil Kit) and the V4 region of the bacterial 16S rRNA gene was amplified using barcoded forward primers (515F, 806R) from the Earth Microbiome Project and http://www.earthmicrobiome.org/protocols-and-standards/16S/ . .. Samples were sequenced by paired end 150 base pair reads at the Harvard Medical School Biopolymers Facility using the MiSeq platform (Illumina).

    Modification:

    Article Title: Effect of Cell Concentration on the Persistence in the Human Intestine of Four Probiotic Strains Administered through a Multispecies Formulation
    Article Snippet: .. After homogenization, 250 mg of feces were weighed and processed using a DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany) for the isolation of total DNA with a minor modification that included incubating samples at 65 °C for 10 min after the addition of the power bead and C1 solutions provided with the kit. .. Steps of mechanical bacterial cell disruption were performed using a Precellys bead beater (Advanced Biotech Italia s.r.l., Seveso, Italy) that was kept in a cold room (3 cycles of 6800 rpm × 30 s).

    Gel Extraction:

    Article Title: Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection
    Article Snippet: 3 DNA extraction, PCR amplification and high-throughput sequencing Genomic DNA was extracted with the DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Valencia, CA), following the manufacturer's instructions. .. The PCR products were purified using a QIAquick Gel Extraction Kit (QIAGEN) and quantified using a Qubit 2.0 Fluorometic High Sensitivity dsDNA Assay (Life Technologies).

    Lysis:

    Article Title: The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters
    Article Snippet: .. Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer. .. The specimens were heated at 65°C for 10 min, incubated at 95°C for 10 min, and processed per PowerSoil instructions.

    Mouse Assay:

    Article Title: Oleoylethanolamide treatment affects gut microbiota composition and the expression of intestinal cytokines in Peyer’s patches of mice
    Article Snippet: For each group of mice, the pellets were collected in sterile conditions and stored at −80 °C until extraction of nucleic acids. .. The bacterial genomic DNA extraction was carried out with DNeasy PowerLyzer PowerSoil Kit (Qiagen, Hilden, Germania) following the manufacturer’s instructions.

    Software:

    Article Title: Effect of Cell Concentration on the Persistence in the Human Intestine of Four Probiotic Strains Administered through a Multispecies Formulation
    Article Snippet: After homogenization, 250 mg of feces were weighed and processed using a DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany) for the isolation of total DNA with a minor modification that included incubating samples at 65 °C for 10 min after the addition of the power bead and C1 solutions provided with the kit. .. After isolation, DNA was quantified, and the 260/280 ratio was measured with a Take3 Micro-Volume analyzed in a microplate reader using Gen5 software (BioTek Instruments, Inc., Winooski, VT, USA).

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    Qiagen dneasy powerlyzer powersoil dna isolation kit
    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = <t>DNeasy</t> <t>PowerLyzer</t> <t>PowerSoil</t> <t>DNA</t> Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Dneasy Powerlyzer Powersoil Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy powerlyzer powersoil dna isolation kit/product/Qiagen
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    dneasy powerlyzer powersoil dna isolation kit - by Bioz Stars, 2020-04
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    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Journal: Scientific Reports

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    doi: 10.1038/s41598-019-55568-y

    Figure Lengend Snippet: nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Article Snippet: DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method .

    Techniques: DNA Extraction

    Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Journal: Scientific Reports

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    doi: 10.1038/s41598-019-55568-y

    Figure Lengend Snippet: Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Article Snippet: DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method .

    Techniques: DNA Extraction