dneasy powerlyzer powersoil dna isolation kit  (Qiagen)

 
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    Name:
    DNeasy PowerLyzer PowerSoil Kit
    Description:
    For the bead based isolation of DNA from tough soil microbes Kit contents For the isolation of DNA from tough soil microbes optimized for use with bead based homogenizers Benefits Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid Ready to use high quality DNA for downstream applications Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minu
    Catalog Number:
    12855-100
    Price:
    550
    Category:
    DNeasy PowerLyzer PowerSoil Kit
    Buy from Supplier


    Structured Review

    Qiagen dneasy powerlyzer powersoil dna isolation kit
    DNeasy PowerLyzer PowerSoil Kit
    For the bead based isolation of DNA from tough soil microbes Kit contents For the isolation of DNA from tough soil microbes optimized for use with bead based homogenizers Benefits Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid Ready to use high quality DNA for downstream applications Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minu
    https://www.bioz.com/result/dneasy powerlyzer powersoil dna isolation kit/product/Qiagen
    Average 99 stars, based on 309 article reviews
    Price from $9.99 to $1999.99
    dneasy powerlyzer powersoil dna isolation kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "DNA extraction approaches substantially influence the assessment of the human breast milk microbiome"

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-55568-y

    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Figure Legend Snippet: nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Techniques Used: DNA Extraction

    Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Figure Legend Snippet: Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Techniques Used: DNA Extraction

    Related Articles

    DNA Extraction:

    Article Title: Oleoylethanolamide treatment affects gut microbiota composition and the expression of intestinal cytokines in Peyer’s patches of mice
    Article Snippet: .. The bacterial genomic DNA extraction was carried out with DNeasy PowerLyzer PowerSoil Kit (Qiagen, Hilden, Germania) following the manufacturer’s instructions. ..

    Article Title: Short-Term Amoxicillin-Induced Perturbation of the Gut Microbiota Promotes Acute Intestinal Immune Regulation in Brown Norway Rats
    Article Snippet: .. Bacterial DNA Extraction DNA was extracted from up to 200 mg of feces and cecum content and up to 250 mg of small intestine content by DNeasy PowerLyzer PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacture’s protocol. .. Mechanical lysis of bacteria was conducted at 30 cycles/s twice for 5 min on bead beater MM300 (Retsch, VWR, Haan, Germany).

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome
    Article Snippet: .. DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method . ..

    Article Title: Urbanization Altered Bacterial and Archaeal Composition in Tidal Freshwater Wetlands Near Washington DC, USA, and Buenos Aires, Argentina
    Article Snippet: .. DNA Extraction and Illumina Library Preparation DNA extractions were carried out using the Qiagen DNeasy PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany). ..

    Lysis:

    Article Title: The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters
    Article Snippet: .. Then 5ml of MO BIO lysis buffer (PowerLyzer PowerSoil Bead Solution, 12855-50-BS) was added to the Falcon tube, and the specimen was homogenized by vortexing for 30–40 s. The Falcon tube was centrifuged for 5 min at 1500 relative centrifugal force, and 1 ml of supernatant was pipetted into 4 barcoded MO BIO Garnet Bead tubes containing 750 μl of MO BIO buffer. .. The specimens were heated at 65°C for 10 min, incubated at 95°C for 10 min, and processed per PowerSoil instructions.

    Incubation:

    Article Title: Gut Feelings Begin in Childhood: the Gut Metagenome Correlates with Early Environment, Caregiving, and Behavior
    Article Snippet: .. DNA was extracted from 250-μl aliquots of the OMNIgene-Gut samples using the MoBio PowerLyzer PowerSoil kit (Qiagen, Hilden, Germany) with the following protocol modifications: following the addition of solution C1, a 1-minute bead-beating step was performed on a Mini-BeadBeater-96 (BioSpec Products, Bartlesville, OK, USA), followed by a 10-minute incubation at 65°C; in the final step, DNA was eluted in two stages for a combined total of 100 μl. ..

    other:

    Article Title: A taxonomic signature of obesity in a large study of American adults
    Article Snippet: DNA was extracted from stool using the PowerLyzer PowerSoil Kit (Mo Bio Laboratory Inc., CA) following manufacturer’s protocol, as described previously .

    Article Title: Microbial Community Patterns Associated with Automated Teller Machine Keypads in New York City
    Article Snippet: We extracted DNA using a MoBio PowerLyzer PowerSoil extraction kit (catalog no. 12855-100) according to manufacturer instructions.

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  • 99
    Qiagen dneasy powerlyzer powersoil dna isolation kit
    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = <t>DNeasy</t> <t>PowerLyzer</t> <t>PowerSoil</t> <t>DNA</t> Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.
    Dneasy Powerlyzer Powersoil Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy powerlyzer powersoil dna isolation kit/product/Qiagen
    Average 99 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    dneasy powerlyzer powersoil dna isolation kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Journal: Scientific Reports

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    doi: 10.1038/s41598-019-55568-y

    Figure Lengend Snippet: nMDS plot of Weighted UniFrac distance matrix for all human breast milk samples and repeats across methods. PERMANOVA revealed significant differences between samples (Pseudo-F = 109.47, P = 0.0001) and between methods (Pseudo-F = 15.162, P = 0.0001). Samples are indicated by colour and method by abbreviation. PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Article Snippet: DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method .

    Techniques: DNA Extraction

    Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Journal: Scientific Reports

    Article Title: DNA extraction approaches substantially influence the assessment of the human breast milk microbiome

    doi: 10.1038/s41598-019-55568-y

    Figure Lengend Snippet: Mock breast milk relative abundance output across methods and repeats compared to expected. Mock breast milk relative abundance ( a ), spurious bacteria not added into mock ( b ), adjusted abundance with spurious bacteria removed ( c ). PS = DNeasy PowerLyzer PowerSoil DNA Isolation, SA = Sigma-Aldrich GenElute Bacterial Genomic DNA Kit, MAN = manual phenol-chloroform extraction method, MK = QIAamp DNA Stool Mini Kit, MKBB = QIAamp DNA Stool Mini Kit with bead beating.

    Article Snippet: DNA extraction methodologies Five DNA extraction methods were assessed: DNeasy PowerLyzer PowerSoil DNA Isolation Kit (QIAGEN, Hilden, Germany); GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Missouri, USA); QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany); QIAamp DNA Stool Mini Kit with the addition of bead-beating; and a manual phenol-chloroform based extraction method .

    Techniques: DNA Extraction