qiaamp powerfecal kit  (Qiagen)


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    Name:
    QIAamp PowerFecal DNA Kit
    Description:
    For the isolation of DNA from stool, gut material and biosolids. Kit contents: For the isolation of DNA from stool, gut material and biosolids. Benefits: High-quality DNA from up to 250 mg samples in just 30 minutes . Optimized for tough DNA samples from stool, biosolids and gut material . Efficient removal of downstream inhibitors with Inhibitor Removal Technology
    Catalog Number:
    12830-50
    Price:
    None
    Category:
    QIAamp PowerFecal DNA Kit
    Score:
    85
    Buy from Supplier


    Structured Review

    Qiagen qiaamp powerfecal kit
    QIAamp PowerFecal DNA Kit
    For the isolation of DNA from stool, gut material and biosolids. Kit contents: For the isolation of DNA from stool, gut material and biosolids. Benefits: High-quality DNA from up to 250 mg samples in just 30 minutes . Optimized for tough DNA samples from stool, biosolids and gut material . Efficient removal of downstream inhibitors with Inhibitor Removal Technology
    https://www.bioz.com/result/qiaamp powerfecal kit/product/Qiagen
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    qiaamp powerfecal kit - by Bioz Stars, 2019-12
    99/100 stars

    Images

    1) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    Related Articles

    Centrifugation:

    Article Title: Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR
    Article Snippet: The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany). .. The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany).

    Amplification:

    Article Title: The impact of Rhodiola rosea on the gut microbial community of Drosophila melanogaster
    Article Snippet: All samples were DNA extracted, amplified, and sequenced by the Integrated Microbiome Resource lab (IMR) at Dalhousie University (Halifax, Canada). .. In brief, DNA was extracted from 5 mg of frozen flies per sample using the QIAamp PowerFecal DNA Kit (Qiagen) per manufacturer’s protocol.

    Article Title: Modulation of Gut Microbiota by Lonicera caerulea L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet
    Article Snippet: An equal amount of feces from each group was washed by DNase-free water to clean the surface, and then fecal DNA was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Venlo, Netherlands), following the manufacturer’s manual. .. PCR cycling conditions consisted of an initial denaturation of 2 min at 94 °C, and 25 cycles of 30 s at 94 °C, 30 s at 55 °C, and 30 s at 72 °C.

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Therefore we aimed to develop a DNA isolation protocol that recovered bacterial DNA of suitable quality for the reliable amplification of H. pylori housekeeping genes. .. Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample.

    Article Title: Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals
    Article Snippet: DNA was extracted using a Mobio PowerFecal® extraction kit (QIAGEN, Germany) according to the manufacturer's protocol. .. 16S rRNA sequencing primers , provided by the IMR, were modified from the primers used by Comeau et . al . to improve compatibility with the Illumina platform [ ].

    Article Title: R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease
    Article Snippet: Paragraph title: 16S rRNA gene amplification and sequencing ... Total DNA was extracted using a PowerFecal DNA isolation kit (MO BIO Laboratories).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses
    Article Snippet: The mixture was immediately spread onto each end of a precoated slide and covered with a fresh glass coverslip, and the comets were captured with an Olympus microscope equipped with a charge-coupled device camera connected to a fluorescent microscope. .. Genomic DNA was extracted using an QIAamp DNA isolation kit (cat. no. 12830-50; Qiagen) and 10 µg DNA from cells was incubated with a 8-OHdG ELISA kit (cat. no. ALX-480-090-M001; Enzo Life Sciences, Inc., Farmingdale, NY), according to the manufacturer's instruction. .. The levels of TNF-α (cat. no. E-EL-H0109c), IL-6 (catalog no. E-EL-H0102c) and IL-1β (cat. no. E-EL-H0149c) were detected using a bioactive ELISA assay in supernatant according to the manufacturer's instructions (Elabscience Biotechnology Co., Ltd., Wuhan, China).

    Real-time Polymerase Chain Reaction:

    Article Title: MHC variation sculpts individualized microbial communities that control susceptibility to enteric infection
    Article Snippet: Paragraph title: Quantitative PCR ... Template extraction for quantification of faecal bacteria loads: DNA was extracted from fresh faecal pellets using the PowerFecal DNA Isolation Kit (Mo Bio) following kit instructions.

    Article Title: Disparate Antibiotic Resistance Gene Quantities Revealed across 4 Major Cities in California: A Survey in Drinking Water, Air, and Soil at 24 Public Parks
    Article Snippet: All drinking water and soil DNA extractions were completed using the MoBio PowerFecal DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) within 1 week of collection. .. All drinking water and soil DNA extractions were completed using the MoBio PowerFecal DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) within 1 week of collection.

    Incubation:

    Article Title: Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains
    Article Snippet: The resulting cultures were stored at −80°C as glycerol stocks. .. For genomic DNA extraction, glycerol stocks were inoculated and streaked on BHI agar plates with 10% sheep blood and incubated at 37°C for 16 h. Colonies were harvested and used for genomic DNA extraction with the QIAamp PowerFecal DNA kit (Qiagen, Hilden, Germany). .. A B. anthracis strain isolated from a human case of anthrax was provided by the School of Veterinary Medicine, University of Zambia, Zambia.

    Article Title: Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses
    Article Snippet: The mixture was immediately spread onto each end of a precoated slide and covered with a fresh glass coverslip, and the comets were captured with an Olympus microscope equipped with a charge-coupled device camera connected to a fluorescent microscope. .. Genomic DNA was extracted using an QIAamp DNA isolation kit (cat. no. 12830-50; Qiagen) and 10 µg DNA from cells was incubated with a 8-OHdG ELISA kit (cat. no. ALX-480-090-M001; Enzo Life Sciences, Inc., Farmingdale, NY), according to the manufacturer's instruction. .. The levels of TNF-α (cat. no. E-EL-H0109c), IL-6 (catalog no. E-EL-H0102c) and IL-1β (cat. no. E-EL-H0149c) were detected using a bioactive ELISA assay in supernatant according to the manufacturer's instructions (Elabscience Biotechnology Co., Ltd., Wuhan, China).

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Briefly, 150 mg of fecal pellets were added to PowerBead Tubes containing 750 μL of bead solution and vortexed to begin homogenization.

    Modification:

    Article Title: Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals
    Article Snippet: DNA was extracted using a Mobio PowerFecal® extraction kit (QIAGEN, Germany) according to the manufacturer's protocol. .. DNA samples were submitted for 16S rRNA gene sequencing at the Integrated Microbiome Resource (IMR) at Dalhousie University.

    Infection:

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Lack of amplification of a ppa fragment with DNA from all infected individuals suggested that the concentration of H. pylori DNA in the stool samples was very low and that there was a need to evaluate other methods to isolate H. pylori specific DNA from the stool specimens. .. Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample.

    other:

    Article Title: Probiotic supplements prevented oxonic acid-induced hyperuricemia and renal damage
    Article Snippet: Charlottetown, PE.

    Polymerase Chain Reaction:

    Article Title: A Comparative Study on the Faecal Bacterial Community and Potential Zoonotic Bacteria of Muskoxen (Ovibos moschatus) in Northeast Greenland, Northwest Greenland and Norway
    Article Snippet: The outermost surface ( > 2 mm) of faecal samples was removed via sterile scalpel before 0.25 g of faecal samples underwent DNA extraction using a QIAamp PowerFecal DNA kit (Qiagen, Hilden, Germany) as per manufacturer’s instructions. .. The outermost surface ( > 2 mm) of faecal samples was removed via sterile scalpel before 0.25 g of faecal samples underwent DNA extraction using a QIAamp PowerFecal DNA kit (Qiagen, Hilden, Germany) as per manufacturer’s instructions.

    Article Title: Modulation of Gut Microbiota by Lonicera caerulea L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet
    Article Snippet: An equal amount of feces from each group was washed by DNase-free water to clean the surface, and then fecal DNA was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Venlo, Netherlands), following the manufacturer’s manual. .. The V3–V4 region of the 16S rRNA gene was amplified using forward primer 341 F (5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-NNNNN-CCTACGGGNGGCWGCAG-3′) and reverse primer 805 R (5′-GTGACTGGAGTTCA GACGTGTGCTCTTCCGATCT-NNNNN-GACTACHVGGGTATCTAATCC-3′).

    Article Title: Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro
    Article Snippet: DNA extraction was then carried out using Power Fecal DNA isolation kit (#12830-50, MO BIO Laboratories) according to manufacturer’s instructions. .. DNA extraction was then carried out using Power Fecal DNA isolation kit (#12830-50, MO BIO Laboratories) according to manufacturer’s instructions.

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: In addition, multiple non-specific PCR products were amplified on some occasions ( B). .. Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample.

    Article Title: Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures
    Article Snippet: DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA). .. Illumina iTag PCR mixtures (25 μl) contained approximately 5 to 10 ng of template DNA with a final concentration of 1× PCR buffer, 0.8 mM deoxynucleoside triphosphates, 0.625 U of Taq polymerase, 0.2 μM barcoded 515F forward primer, and 0.2 μM Illumina 806R reverse primer per reaction mixture ( ).

    DNA Extraction:

    Article Title: A Comparative Study on the Faecal Bacterial Community and Potential Zoonotic Bacteria of Muskoxen (Ovibos moschatus) in Northeast Greenland, Northwest Greenland and Norway
    Article Snippet: No 55se-50190-00153-15, in 2013). .. The outermost surface ( > 2 mm) of faecal samples was removed via sterile scalpel before 0.25 g of faecal samples underwent DNA extraction using a QIAamp PowerFecal DNA kit (Qiagen, Hilden, Germany) as per manufacturer’s instructions. .. Bacterial metabarcoding was performed on the V3-V4 region of the 16S rRNA region, using the 341F (5′-CCTAYGGGRBGCASCAG-3′) and reverse 806R (5′-GGACTACNNGGGTATCTAAT-3′) primers [ ].

    Article Title: Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains
    Article Snippet: The resulting cultures were stored at −80°C as glycerol stocks. .. For genomic DNA extraction, glycerol stocks were inoculated and streaked on BHI agar plates with 10% sheep blood and incubated at 37°C for 16 h. Colonies were harvested and used for genomic DNA extraction with the QIAamp PowerFecal DNA kit (Qiagen, Hilden, Germany). .. A B. anthracis strain isolated from a human case of anthrax was provided by the School of Veterinary Medicine, University of Zambia, Zambia.

    Article Title: Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses
    Article Snippet: The mixture was immediately spread onto each end of a precoated slide and covered with a fresh glass coverslip, and the comets were captured with an Olympus microscope equipped with a charge-coupled device camera connected to a fluorescent microscope. .. Genomic DNA was extracted using an QIAamp DNA isolation kit (cat. no. 12830-50; Qiagen) and 10 µg DNA from cells was incubated with a 8-OHdG ELISA kit (cat. no. ALX-480-090-M001; Enzo Life Sciences, Inc., Farmingdale, NY), according to the manufacturer's instruction. .. The levels of TNF-α (cat. no. E-EL-H0109c), IL-6 (catalog no. E-EL-H0102c) and IL-1β (cat. no. E-EL-H0149c) were detected using a bioactive ELISA assay in supernatant according to the manufacturer's instructions (Elabscience Biotechnology Co., Ltd., Wuhan, China).

    Article Title: Methodology challenges in studying human gut microbiota – effects of collection, storage, DNA extraction and next generation sequencing technologies
    Article Snippet: Briefly, the faeces sample was thoroughly mixed with 2 mL of stabilization liquid in the provided tube. .. Three commercial faecal DNA extraction kits were evaluated in this study: Power Fecal DNA Isolation Kit (MO BIO, #12830-50), QIAamp Fast DNA Stool Mini Kit (QIAGEN, #51604) and Fast DNA SPIN Kit for Feces (MP Biomedicals, #116570200). .. The manufacturers’ protocols were followed for each kit, and recommendations for application to hard-to-lyse organisms were incorporated (detailed protocols for each DNA extraction procedure are supplied in Supplementary Methods).

    Article Title: Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro
    Article Snippet: Samples were centrifuged once more, and pellets were resuspended in 750 μL bead solution and transferred to bead beating tubes (as supplied). .. DNA extraction was then carried out using Power Fecal DNA isolation kit (#12830-50, MO BIO Laboratories) according to manufacturer’s instructions. .. The 16S rRNA V4 variable region was sequenced using the 515/806 primer set with barcode on the forward primer.

    Article Title: Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR
    Article Snippet: The kits, which used silica spin filter technology, were chosen for their easy and fast extraction methods. .. The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany). .. For NucleoSpin® Soil, sample lysis was performed with the optional enhancer SX solution, as recommended by the manufacturer.

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Paragraph title: 3.1. Protocol development for H. pylori specific DNA isolation from stool specimens ... Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample.

    Article Title: Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures
    Article Snippet: Untreated and treated samples were then frozen at −80°C and shipped on dry ice to the Lamendella laboratory for further processing. .. DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA). .. Illumina iTag PCR mixtures (25 μl) contained approximately 5 to 10 ng of template DNA with a final concentration of 1× PCR buffer, 0.8 mM deoxynucleoside triphosphates, 0.625 U of Taq polymerase, 0.2 μM barcoded 515F forward primer, and 0.2 μM Illumina 806R reverse primer per reaction mixture ( ).

    Article Title: Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals
    Article Snippet: Paragraph title: DNA extraction and sequencing ... DNA was extracted using a Mobio PowerFecal® extraction kit (QIAGEN, Germany) according to the manufacturer's protocol.

    Article Title: MHC variation sculpts individualized microbial communities that control susceptibility to enteric infection
    Article Snippet: Abundance estimates are standardized to the concentration of input DNA per reaction and are represented as copies per nanogram of faecal DNA. .. Template extraction for quantification of faecal bacteria loads: DNA was extracted from fresh faecal pellets using the PowerFecal DNA Isolation Kit (Mo Bio) following kit instructions. .. Bacterial loads were quantified using previously validated bacterial group-specific 16S primers ( ).

    Article Title: Use of Metagenomic Shotgun Sequencing Technology To Detect Foodborne Pathogens within the Microbiome of the Beef Production Chain
    Article Snippet: The combined samples were then centrifuged (4,300 × g , 20 min, 4°C), and the pellet was collected for DNA extraction. .. DNA was extracted from 250 mg each of water, composite sponge, and trimming rinsate pellet using the Mo Bio PowerFecal DNA isolation kit (Mo Bio Laboratories, Inc.), according to the manufacturer's protocol. .. Extracted DNA from fecal and soil samples was eluted in 5 ml of the kit elution buffer, and water, sponge, and trimming rinsate samples were eluted in 50 μl of the kit elution buffer.

    Article Title: Effect of predatory bacteria on the gut bacterial microbiota in rats
    Article Snippet: To determine the effect of predatory bacteria on the commensal gut bacterial microbiota, feces were collected from five rats from each treatment group for each day for up to seven days post-instillation and frozen immediately at −80 °C. .. DNA from feces was extracted using the PowerFecal DNA Isolation Kit (Mo Bio Laboratories). .. DNA quantity and quality was assessed using an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA).

    Article Title: R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease
    Article Snippet: Fresh fecal pellets were collected from each mouse. .. Total DNA was extracted using a PowerFecal DNA isolation kit (MO BIO Laboratories). .. The V3–V4 variable region of the 16S rRNA gene was amplified from fecal DNA extracts using the 16S metagenomic sequencing library protocol provided by Illumina.

    Article Title: Disparate Antibiotic Resistance Gene Quantities Revealed across 4 Major Cities in California: A Survey in Drinking Water, Air, and Soil at 24 Public Parks
    Article Snippet: Sample tubes were placed on ice and kept at 4 °C to the extent possible before transport to the laboratory, where they were stored at −20 °C prior to DNA extraction. .. All drinking water and soil DNA extractions were completed using the MoBio PowerFecal DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) within 1 week of collection. .. Extractions proceeded as per the manufacturer’s guidelines except for the cell lysis step, where bead-beating for 2 min via a BioSpec MiniBeadbeater-8 (BioSpec Products, Bartlesville, OK) was used in place of vortexing.

    Article Title: Hologenomic adaptations underlying the evolution of sanguivory in the common vampire bat
    Article Snippet: We have complied with all relevant ethical regulations for the collection of these samples. .. DNA from fecal samples was extracted using the PowerFecal DNA Isolation Kit (MoBio) with modifications to the manufacturer’s instructions. .. DNA from the D. rotundus milk sample was extracted using a standard commercial kit (Qiagen), following manufacturer's instructions.

    Isolation:

    Article Title: Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains
    Article Snippet: Isolation of B. anthracis strains from samples was performed as described previously ( ). .. For genomic DNA extraction, glycerol stocks were inoculated and streaked on BHI agar plates with 10% sheep blood and incubated at 37°C for 16 h. Colonies were harvested and used for genomic DNA extraction with the QIAamp PowerFecal DNA kit (Qiagen, Hilden, Germany).

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: After mice had transitioned into individual housing for downstream behavioral testing, 1–2 g of fecal pellets were collected from cage bedding into a sterile 2 mL microcentrifuge tube using tweezers which were sterilized between subjects. .. Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. DNA extraction was performed following the manufacturer’s instructions with modification to input volume.

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: An antibody capture based technique to enrich for H. pylori organisms present in fecal slurry prior to isolation of DNA was used. .. Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample.

    Size-exclusion Chromatography:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Briefly, 150 mg of fecal pellets were added to PowerBead Tubes containing 750 μL of bead solution and vortexed to begin homogenization.

    Purification:

    Article Title: Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro
    Article Snippet: DNA extraction was then carried out using Power Fecal DNA isolation kit (#12830-50, MO BIO Laboratories) according to manufacturer’s instructions. .. DNA extraction was then carried out using Power Fecal DNA isolation kit (#12830-50, MO BIO Laboratories) according to manufacturer’s instructions.

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample. .. However, products could still not be amplified from DNA isolated from all infected individuals with all primers.

    Article Title: Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures
    Article Snippet: DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA). .. DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA).

    Article Title: Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals
    Article Snippet: DNA was extracted using a Mobio PowerFecal® extraction kit (QIAGEN, Germany) according to the manufacturer's protocol. .. DNA was extracted using a Mobio PowerFecal® extraction kit (QIAGEN, Germany) according to the manufacturer's protocol.

    Sequencing:

    Article Title: The impact of Rhodiola rosea on the gut microbial community of Drosophila melanogaster
    Article Snippet: Paragraph title: 16S rRNA gene sequencing ... In brief, DNA was extracted from 5 mg of frozen flies per sample using the QIAamp PowerFecal DNA Kit (Qiagen) per manufacturer’s protocol.

    Article Title: Modulation of Gut Microbiota by Lonicera caerulea L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet
    Article Snippet: Paragraph title: 4.5. Characterization of Gut Microbiota by 16S rRNA Gene Sequencing ... An equal amount of feces from each group was washed by DNase-free water to clean the surface, and then fecal DNA was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Venlo, Netherlands), following the manufacturer’s manual.

    Article Title: Prebiotics Modulate the Effects of Antibiotics on Gut Microbial Diversity and Functioning in Vitro
    Article Snippet: Paragraph title: 2.3. DNA Extraction and 16S Sequencing ... DNA extraction was then carried out using Power Fecal DNA isolation kit (#12830-50, MO BIO Laboratories) according to manufacturer’s instructions.

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample. .. However, products could still not be amplified from DNA isolated from all infected individuals with all primers.

    Article Title: Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures
    Article Snippet: DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA). .. DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA).

    Article Title: Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals
    Article Snippet: Paragraph title: DNA extraction and sequencing ... DNA was extracted using a Mobio PowerFecal® extraction kit (QIAGEN, Germany) according to the manufacturer's protocol.

    Article Title: R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease
    Article Snippet: Paragraph title: 16S rRNA gene amplification and sequencing ... Total DNA was extracted using a PowerFecal DNA isolation kit (MO BIO Laboratories).

    Article Title: Metagenomic Analysis of Bacteria, Fungi, Bacteriophages, and Helminths in the Gut of Giant Pandas
    Article Snippet: Paragraph title: Extraction of DNA and Sequencing ... DNA was extracted from fecal samples by using the PowerFecal® DNA Isolation Kit (MO BIO Laboratories, Inc.) following the manufacturer’s instructions.

    Gel Extraction:

    Article Title: Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures
    Article Snippet: DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA). .. Each PCR was carried out with an MJ Research PTC-200 thermocycler (Bio-Rad, Hercules, CA).

    Chromatin Immunoprecipitation:

    Article Title: Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures
    Article Snippet: DNA was extracted from approximately 680 μl of a homogenized fecal solution ( n = 176) with a Mo Bio PowerFecal DNA extraction kit in accordance with the manufacturer’s instructions (Mo Bio Laboratories, Carlsbad, CA). .. Pooled PCR products were gel purified with a QIAquick gel extraction kit (Qiagen, Frederick, MD) and quantified with a Qubit 2.0 fluorometer (Life Technologies, Inc., Carlsbad, CA).

    SYBR Green Assay:

    Article Title: MHC variation sculpts individualized microbial communities that control susceptibility to enteric infection
    Article Snippet: All quantitative PCR reactions were conducted in 12.5 μl volumes using the SYBR green Master Mix (Roche). .. Template extraction for quantification of faecal bacteria loads: DNA was extracted from fresh faecal pellets using the PowerFecal DNA Isolation Kit (Mo Bio) following kit instructions.

    Selection:

    Article Title: Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR
    Article Snippet: Paragraph title: Kit selection and DNA extraction ... The following kits were evaluated: PowerSoil® DNA isolation and PowerFecal® kits (Mo Bio Laboratories Inc., Carlsbad, CA), QIAamp® DNA stool mini kit (Qiagen Inc., Valencia, CA), and NucleoSpin® Soil (Macherey-Nagel, Düren, Germany).

    Homogenization:

    Article Title: Methodology challenges in studying human gut microbiota – effects of collection, storage, DNA extraction and next generation sequencing technologies
    Article Snippet: Three commercial faecal DNA extraction kits were evaluated in this study: Power Fecal DNA Isolation Kit (MO BIO, #12830-50), QIAamp Fast DNA Stool Mini Kit (QIAGEN, #51604) and Fast DNA SPIN Kit for Feces (MP Biomedicals, #116570200). .. Three commercial faecal DNA extraction kits were evaluated in this study: Power Fecal DNA Isolation Kit (MO BIO, #12830-50), QIAamp Fast DNA Stool Mini Kit (QIAGEN, #51604) and Fast DNA SPIN Kit for Feces (MP Biomedicals, #116570200).

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany).

    Ethanol Precipitation:

    Article Title: Use of Metagenomic Shotgun Sequencing Technology To Detect Foodborne Pathogens within the Microbiome of the Beef Production Chain
    Article Snippet: DNA was extracted from 250 mg each of water, composite sponge, and trimming rinsate pellet using the Mo Bio PowerFecal DNA isolation kit (Mo Bio Laboratories, Inc.), according to the manufacturer's protocol. .. DNA concentrations were measured at 260 nm using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Inc., Pittsburgh, PA).

    Spectrophotometry:

    Article Title: MHC variation sculpts individualized microbial communities that control susceptibility to enteric infection
    Article Snippet: Template extraction for quantification of faecal bacteria loads: DNA was extracted from fresh faecal pellets using the PowerFecal DNA Isolation Kit (Mo Bio) following kit instructions. .. Template extraction for quantification of faecal bacteria loads: DNA was extracted from fresh faecal pellets using the PowerFecal DNA Isolation Kit (Mo Bio) following kit instructions.

    Article Title: Use of Metagenomic Shotgun Sequencing Technology To Detect Foodborne Pathogens within the Microbiome of the Beef Production Chain
    Article Snippet: DNA was extracted from 250 mg each of water, composite sponge, and trimming rinsate pellet using the Mo Bio PowerFecal DNA isolation kit (Mo Bio Laboratories, Inc.), according to the manufacturer's protocol. .. DNA was extracted from 250 mg each of water, composite sponge, and trimming rinsate pellet using the Mo Bio PowerFecal DNA isolation kit (Mo Bio Laboratories, Inc.), according to the manufacturer's protocol.

    Concentration Assay:

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Lack of amplification of a ppa fragment with DNA from all infected individuals suggested that the concentration of H. pylori DNA in the stool samples was very low and that there was a need to evaluate other methods to isolate H. pylori specific DNA from the stool specimens. .. Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample.

    Article Title: MHC variation sculpts individualized microbial communities that control susceptibility to enteric infection
    Article Snippet: Abundance estimates are standardized to the concentration of input DNA per reaction and are represented as copies per nanogram of faecal DNA. .. Template extraction for quantification of faecal bacteria loads: DNA was extracted from fresh faecal pellets using the PowerFecal DNA Isolation Kit (Mo Bio) following kit instructions.

    Article Title: Disparate Antibiotic Resistance Gene Quantities Revealed across 4 Major Cities in California: A Survey in Drinking Water, Air, and Soil at 24 Public Parks
    Article Snippet: All drinking water and soil DNA extractions were completed using the MoBio PowerFecal DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) within 1 week of collection. .. Eluted DNA was aliquoted and stored at −20 °C, awaiting qPCR analysis.

    Lysis:

    Article Title: The use of stool specimens reveals Helicobacter pylori strain diversity in a cohort of adolescents and their family members in a developed country
    Article Snippet: Previous studies have reported efficient DNA extraction from clinical specimens, including stool, containing multiple microbial species when a bead beating step was included ( , , ). .. Therefore we evaluated the use of the PowerFecal® kit (Mo Bio) which includes a bead beating step for mechanical lysis of the cells in the sample. .. H. pylori DNA isolated using the modified protocol resulted in improved amplification of fragments of H. pylori MLST housekeeping genes ( C).

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    Qiagen qiaamp powerfecal kit
    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: <t>QIAamp</t> ® <t>PowerFecal</t> ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Qiaamp Powerfecal Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Techniques: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Techniques: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Techniques: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Techniques: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Techniques: DNA Extraction