qiaamp powerfecal kit  (Qiagen)


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    Name:
    QIAamp PowerFecal DNA Kit
    Description:
    For the isolation of DNA from stool gut material and biosolids Kit contents For the isolation of DNA from stool gut material and biosolids Benefits High quality DNA from up to 250 mg samples in just 30 minutes Optimized for tough DNA samples from stool biosolids and gut material Efficient removal of downstream inhibitors with Inhibitor Removal Technology
    Catalog Number:
    12830-50
    Price:
    293
    Category:
    QIAamp PowerFecal DNA Kit
    Buy from Supplier


    Structured Review

    Qiagen qiaamp powerfecal kit
    QIAamp PowerFecal DNA Kit
    For the isolation of DNA from stool gut material and biosolids Kit contents For the isolation of DNA from stool gut material and biosolids Benefits High quality DNA from up to 250 mg samples in just 30 minutes Optimized for tough DNA samples from stool biosolids and gut material Efficient removal of downstream inhibitors with Inhibitor Removal Technology
    https://www.bioz.com/result/qiaamp powerfecal kit/product/Qiagen
    Average 99 stars, based on 1346 article reviews
    Price from $9.99 to $1999.99
    qiaamp powerfecal kit - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    2) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    3) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    4) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    5) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    6) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    7) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    8) Product Images from "Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing"

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202858

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Figure Legend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Techniques Used: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.
    Figure Legend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Techniques Used: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .
    Figure Legend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Techniques Used: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.
    Figure Legend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Techniques Used: DNA Extraction

    Related Articles

    DNA Extraction:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit. .. However, DNA extraction with the Maxwell® RSC kit produced higher concentrations with a lower fecal sample input weight and took a fraction of the time compared to the QIAamp® PowerFecal® protocol.

    Article Title: Altered fecal microbiota composition in the Flinders sensitive line rat model of depression
    Article Snippet: Paragraph title: DNA extraction ... DNA was extracted using QIAamp PowerFecal DNA Kit (including a bead-beating step; #12830-50; Qiagen, Germany) according to the manufacturer’s instructions and sent to DNASense (Aalborg, Denmark) for 16S rRNA amplicon sequencing.

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: .. DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. DNA concentrations of every sample were quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, United States).

    Article Title: Salt-Responsive Metabolite, β-Hydroxybutyrate, Attenuates Hypertension
    Article Snippet: .. DNA extraction and Quantification Fecal DNA was extracted from one fecal pellet (approximately 0.2g) using QIAam®PowerFecal®DNA kit (QIAGEN) followed by provided protocol. ..

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: .. For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ]. .. As a result of the technological advancements made in 16S sequencing, there is a parallel opportunity for DNA extraction methods that take advantage of the numerous automated platforms available.

    Amplification:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit. .. Results showed that the two approaches yielded similar measures of DNA purity and successful next-generation sequencing amplification and produced comparable composition of microbial communities.

    Article Title: Altered fecal microbiota composition in the Flinders sensitive line rat model of depression
    Article Snippet: .. DNA was extracted using QIAamp PowerFecal DNA Kit (including a bead-beating step; #12830-50; Qiagen, Germany) according to the manufacturer’s instructions and sent to DNASense (Aalborg, Denmark) for 16S rRNA amplicon sequencing. .. Library preparation Bacterial V4 16S sequencing libraries were prepared using a custom protocol based on an Illumina protocol (#15044223 Rev.

    Article Title: The impact of Rhodiola rosea on the gut microbial community of Drosophila melanogaster
    Article Snippet: All samples were DNA extracted, amplified, and sequenced by the Integrated Microbiome Resource lab (IMR) at Dalhousie University (Halifax, Canada). .. In brief, DNA was extracted from 5 mg of frozen flies per sample using the QIAamp PowerFecal DNA Kit (Qiagen) per manufacturer’s protocol.

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. The genes of all bacterial 16S rRNA in the region of V3–V4 were amplified by polymerase chain reaction (PCR) using a universal forward primer 338F (5′-ACTCCTRCGGGAGGCAGCAG-3′) and a reverse primer 806R (5′-GGACTACCVGGGTATCTAAT-3′) ( ).

    Ligation:

    Article Title: Does exercise impact gut microbiota composition in men receiving androgen deprivation therapy for prostate cancer? A single-blinded, two-armed, randomised controlled trial
    Article Snippet: To extract microbial DNA, samples will be lysed using both mechanical and enzymatic breakdown of the bacterial cell membranes and the DNA extracted using the QIAamp PowerFecal DNA kit (Qiagen; Hilden, Germany). .. For building the sequence libraries, a PCR-free ligation protocol will be deployed with sample barcodes that are never reused in our laboratory.

    Isolation:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: In addition to rapid advancement in sequencing technologies, automated systems have been developed for nucleic acid extraction; however, these methods have yet to be widely used for the isolation of bacterial DNA from fecal samples. .. Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: .. Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). ..

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: .. Qiagen QIAamp® PowerFecal® Kit Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). ..

    Next-Generation Sequencing:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: As affordable, advanced next-generation sequencing technologies become more widely available, important discoveries about the composition and function of these microbes become increasingly possible. .. Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: .. For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ]. .. As a result of the technological advancements made in 16S sequencing, there is a parallel opportunity for DNA extraction methods that take advantage of the numerous automated platforms available.

    Homogenization:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Briefly, 150 mg of fecal pellets were added to PowerBead Tubes containing 750 μL of bead solution and vortexed to begin homogenization.

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Qiagen QIAamp® PowerFecal® Kit Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Briefly, 150 mg of fecal pellets were added to PowerBead Tubes containing 750 μL of bead solution and vortexed to begin homogenization.

    Sequencing:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: In addition to rapid advancement in sequencing technologies, automated systems have been developed for nucleic acid extraction; however, these methods have yet to be widely used for the isolation of bacterial DNA from fecal samples. .. Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit.

    Article Title: Does exercise impact gut microbiota composition in men receiving androgen deprivation therapy for prostate cancer? A single-blinded, two-armed, randomised controlled trial
    Article Snippet: To extract microbial DNA, samples will be lysed using both mechanical and enzymatic breakdown of the bacterial cell membranes and the DNA extracted using the QIAamp PowerFecal DNA kit (Qiagen; Hilden, Germany). .. Quality control samples are included in the analysis from sample collection to sequence analysis and all samples will be individually screened for PCR efficiency prior to building sequence libraries.

    Article Title: Altered fecal microbiota composition in the Flinders sensitive line rat model of depression
    Article Snippet: .. DNA was extracted using QIAamp PowerFecal DNA Kit (including a bead-beating step; #12830-50; Qiagen, Germany) according to the manufacturer’s instructions and sent to DNASense (Aalborg, Denmark) for 16S rRNA amplicon sequencing. .. Library preparation Bacterial V4 16S sequencing libraries were prepared using a custom protocol based on an Illumina protocol (#15044223 Rev.

    Article Title: The impact of Rhodiola rosea on the gut microbial community of Drosophila melanogaster
    Article Snippet: Paragraph title: 16S rRNA gene sequencing ... In brief, DNA was extracted from 5 mg of frozen flies per sample using the QIAamp PowerFecal DNA Kit (Qiagen) per manufacturer’s protocol.

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: .. DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. DNA concentrations of every sample were quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, United States).

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Fecal samples contain various PCR inhibitors, which can impede the PCR reaction necessary to generate 16S rRNA amplicons prior to sequencing [ ]. .. For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ].

    Size-exclusion Chromatography:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Solution C1 was added and the samples were briefly vortexed before incubation at 65°C for 10 min. To aid in collision of the beads with microbial cells and optimize homogenization of the samples, PowerBead Tubes were horizontally secured to an analog vortex mixer using the MO BIO Vortex Adapter (Catalog No. 13000-V1-24; Scientific Industries, Bohemia, NY), shaken for 10 min, and subsequently centrifuged at 10,000 x g for 30 sec.

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Qiagen QIAamp® PowerFecal® Kit Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Solution C1 was added and the samples were briefly vortexed before incubation at 65°C for 10 min. To aid in collision of the beads with microbial cells and optimize homogenization of the samples, PowerBead Tubes were horizontally secured to an analog vortex mixer using the MO BIO Vortex Adapter (Catalog No. 13000-V1-24; Scientific Industries, Bohemia, NY), shaken for 10 min, and subsequently centrifuged at 10,000 x g for 30 sec.

    Spectrophotometry:

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. DNA concentrations of every sample were quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, United States).

    Mouse Assay:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ]. .. Using fecal samples collected from 40 mice, the present study evaluates the performance of the Qiagen QIAamp® PowerFecal® Kit against Promega’s Maxwell® Rapid Sample Concentrator (RSC) PureFood GMO and Authentication Kit, which we modified for fecal samples.

    Produced:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Here, we adapted Promega’s Maxwell® RSC PureFood GMO and Authentication kit for use with fecal samples and compared it to the commonly used Qiagen QIAamp® PowerFecal® kit. .. Results showed that the two approaches yielded similar measures of DNA purity and successful next-generation sequencing amplification and produced comparable composition of microbial communities.

    Polymerase Chain Reaction:

    Article Title: Does exercise impact gut microbiota composition in men receiving androgen deprivation therapy for prostate cancer? A single-blinded, two-armed, randomised controlled trial
    Article Snippet: To extract microbial DNA, samples will be lysed using both mechanical and enzymatic breakdown of the bacterial cell membranes and the DNA extracted using the QIAamp PowerFecal DNA kit (Qiagen; Hilden, Germany). .. Quality control samples are included in the analysis from sample collection to sequence analysis and all samples will be individually screened for PCR efficiency prior to building sequence libraries.

    Article Title: The impact of Rhodiola rosea on the gut microbial community of Drosophila melanogaster
    Article Snippet: In brief, DNA was extracted from 5 mg of frozen flies per sample using the QIAamp PowerFecal DNA Kit (Qiagen) per manufacturer’s protocol. .. All DNA samples were amplified by PCR targeting the 16S rRNA gene sequence (regions V6–V8) as previously demonstrated [ ], and libraries were prepared by following the guidelines provided by Illumina (San Diego, USA; Part #15044223, Rev.

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. The genes of all bacterial 16S rRNA in the region of V3–V4 were amplified by polymerase chain reaction (PCR) using a universal forward primer 338F (5′-ACTCCTRCGGGAGGCAGCAG-3′) and a reverse primer 806R (5′-GGACTACCVGGGTATCTAAT-3′) ( ).

    Article Title: Salt-Responsive Metabolite, β-Hydroxybutyrate, Attenuates Hypertension
    Article Snippet: DNA extraction and Quantification Fecal DNA was extracted from one fecal pellet (approximately 0.2g) using QIAam®PowerFecal®DNA kit (QIAGEN) followed by provided protocol. .. DNA concentration was measured by NanoDrop and then it was diluted to be 5ng/μl in low TE buffer to proceed the PCR library preparation.

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Fecal samples contain various PCR inhibitors, which can impede the PCR reaction necessary to generate 16S rRNA amplicons prior to sequencing [ ]. .. For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ].

    Incubation:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Solution C1 was added and the samples were briefly vortexed before incubation at 65°C for 10 min. To aid in collision of the beads with microbial cells and optimize homogenization of the samples, PowerBead Tubes were horizontally secured to an analog vortex mixer using the MO BIO Vortex Adapter (Catalog No. 13000-V1-24; Scientific Industries, Bohemia, NY), shaken for 10 min, and subsequently centrifuged at 10,000 x g for 30 sec.

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: Qiagen QIAamp® PowerFecal® Kit Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany). .. Solution C1 was added and the samples were briefly vortexed before incubation at 65°C for 10 min. To aid in collision of the beads with microbial cells and optimize homogenization of the samples, PowerBead Tubes were horizontally secured to an analog vortex mixer using the MO BIO Vortex Adapter (Catalog No. 13000-V1-24; Scientific Industries, Bohemia, NY), shaken for 10 min, and subsequently centrifuged at 10,000 x g for 30 sec.

    Article Title: Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter–gatherers in Scandinavia
    Article Snippet: We performed an extraction on ble008 sample using QIAamp PowerFecal DNA Kit (Qiagen), which is used to remove inhibitors in stool, gut and biosolid samples. .. After adding the reagents according to the protocol and performing the first incubation step, we used a thermoshaker and vortexed the samples at 65 °C for 30 min at the highest speed available.

    Gel Extraction:

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. PCR amplicons were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, United States), according to the manufacturer’s instructions.

    Modification:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ]. .. Using fecal samples collected from 40 mice, the present study evaluates the performance of the Qiagen QIAamp® PowerFecal® Kit against Promega’s Maxwell® Rapid Sample Concentrator (RSC) PureFood GMO and Authentication Kit, which we modified for fecal samples.

    Lysis:

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing
    Article Snippet: In addition, the first step in DNA extraction—disruption or lysis of bacterial membranes—can bias the capture of specific bacterial taxa due to differences in cell wall structure and integrity [ – ]. .. For this reason, procedures outlined in Qiagen’s QIAamp® PowerFecal® Kit (formally the MO BIO PowerFecal DNA kit; MO BIO Laboratories, Carlsbad, CA), which combines bead-based mechanical disruption with heat exposure have emerged as the preferred method for fecal sample DNA extraction involving downstream NGS [ ].

    Purification:

    Article Title: Microbiome-Metabolomics Analysis Investigating the Impacts of Dietary Starch Types on the Composition and Metabolism of Colonic Microbiota in Finishing Pigs
    Article Snippet: DNA Extraction, Illumina MiSeq Sequencing, and Data Processing Total genomic DNA from the individual samples of colonic digesta was extracted using a QIAamp PowerFecal DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. PCR amplicons were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, United States), according to the manufacturer’s instructions.

    Article Title: Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter–gatherers in Scandinavia
    Article Snippet: The extract was purified using MinElute spin columns and a buffer set (both Qiagen). .. We performed an extraction on ble008 sample using QIAamp PowerFecal DNA Kit (Qiagen), which is used to remove inhibitors in stool, gut and biosolid samples.

    Concentration Assay:

    Article Title: Salt-Responsive Metabolite, β-Hydroxybutyrate, Attenuates Hypertension
    Article Snippet: DNA extraction and Quantification Fecal DNA was extracted from one fecal pellet (approximately 0.2g) using QIAam®PowerFecal®DNA kit (QIAGEN) followed by provided protocol. .. DNA concentration was measured by NanoDrop and then it was diluted to be 5ng/μl in low TE buffer to proceed the PCR library preparation.

    Generated:

    Article Title: Does exercise impact gut microbiota composition in men receiving androgen deprivation therapy for prostate cancer? A single-blinded, two-armed, randomised controlled trial
    Article Snippet: To extract microbial DNA, samples will be lysed using both mechanical and enzymatic breakdown of the bacterial cell membranes and the DNA extracted using the QIAamp PowerFecal DNA kit (Qiagen; Hilden, Germany). .. Microbiome signatures will be generated using the Illumina MiSeq platform using barcoded primer, V4 (515–806).

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    Qiagen authentication kit against qiagen s qiaamp powerfecal kit
    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: <t>QIAamp</t> ® <t>PowerFecal</t> ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).
    Authentication Kit Against Qiagen S Qiaamp Powerfecal Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Article Snippet: DNA extraction method impacts DNA yield To compare the suitability of Promega’s modified Maxwell® RSC PureFood GMO and Authentication Kit against Qiagen’s QIAamp® PowerFecal® Kit for extraction of bacterial DNA from fecal samples, boli from 40 mice were processed with each method according to the protocols outlined above.

    Techniques: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Article Snippet: DNA extraction method impacts DNA yield To compare the suitability of Promega’s modified Maxwell® RSC PureFood GMO and Authentication Kit against Qiagen’s QIAamp® PowerFecal® Kit for extraction of bacterial DNA from fecal samples, boli from 40 mice were processed with each method according to the protocols outlined above.

    Techniques: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of most prominent genera. Bacterial genera with a relative abundance ≥ 0.01 (1%) are visualized here. Relative abundances (mean ± s.d.) for the top 30 most abundant genera are available in S2 Table .

    Article Snippet: DNA extraction method impacts DNA yield To compare the suitability of Promega’s modified Maxwell® RSC PureFood GMO and Authentication Kit against Qiagen’s QIAamp® PowerFecal® Kit for extraction of bacterial DNA from fecal samples, boli from 40 mice were processed with each method according to the protocols outlined above.

    Techniques: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n = 39) revealed similar proportions of the 9 most prominent phyla. Relative abundances (mean ± s.d.) are available in S1 Table .

    Article Snippet: DNA extraction method impacts DNA yield To compare the suitability of Promega’s modified Maxwell® RSC PureFood GMO and Authentication Kit against Qiagen’s QIAamp® PowerFecal® Kit for extraction of bacterial DNA from fecal samples, boli from 40 mice were processed with each method according to the protocols outlined above.

    Techniques: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Article Snippet: DNA extraction method impacts DNA yield To compare the suitability of Promega’s modified Maxwell® RSC PureFood GMO and Authentication Kit against Qiagen’s QIAamp® PowerFecal® Kit for extraction of bacterial DNA from fecal samples, boli from 40 mice were processed with each method according to the protocols outlined above.

    Techniques: DNA Extraction

    Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Weighted Unifrac principal coordinate analysis (PCoA) of samples with successful amplification and sequencing. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ).

    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany).

    Techniques: Amplification, Sequencing, DNA Extraction

    Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Procrustes-based comparison of microbial composition where longer lines indicate more within-subject dissimilarity of the microbiome. Colors denote DNA extraction method: QIAamp ® PowerFecal ® Kit ( red ) and Maxwell ® RSC PureFood GMO and Authentication Kit ( blue ). Numbers 1–5 denote the 5 technical replicate pairs sequenced with each kit.

    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany).

    Techniques: DNA Extraction

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the genus level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany).

    Techniques: DNA Extraction, Next-Generation Sequencing

    Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Comparison of DNA extraction methods on next generation sequencing (NGS) relative abundance at the phylum level. NGS using DNA extracted with the QIAamp ® PowerFecal ® Kit ( n = 40) and the Maxwell ® RSC PureFood GMO and Authentication Kit ( n .

    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany).

    Techniques: DNA Extraction, Next-Generation Sequencing

    Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Journal: PLoS ONE

    Article Title: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

    doi: 10.1371/journal.pone.0202858

    Figure Lengend Snippet: Alpha diversity metrics compared across DNA extraction methods. (A) Box plot comparing number of observed operational taxonomic units (OTUs) across kits. (B) Box plot of Chao1 diversity estimates for the microbial communities in DNA extracted with both the QIAamp ® PowerFecal ® Kit and the Maxwell ® RSC PureFood GMO and Authentication Kit. (C) Box plot of Shannon diversity index comparing the evenness and abundance of species extracted with the two kits.

    Article Snippet: Genomic DNA was isolated from fecal samples using the Qiagen QIAamp® PowerFecal® Kit (Catalog No. 12830–50; Qiagen, Hilden, Germany).

    Techniques: DNA Extraction