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(A) Western blot showing a time course of PARP7 and C/EBPβ expression in 3T3-L1 cells differentiated with MDI cocktail. (B) Bar graphs showing the enrichment of C/EBPβ at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells with or without differentiation using MDI cocktail. Mean + SEM; n = 3. Asterisks indicate significant differences from control; ANOVA; * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001. (C) IP-western assays showing interactions between C/EBPβ and PARP7 in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-C/EBPβ were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG and PARP7. (D) Western blots of chromatin fractions from 3T3-L1 cells showing two p300-mediated histone modifications, H3K27ac and <t>H2BK5ac,</t> as well as H3K27me3, in bulk histones. PARP7 input confirms expected stabilization (2 nd lane from left) and knockdown (3 rd lane from left) of PARP7. (E) IP-western assays showing interactions between PARP7, p300, and C/EBPβ in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-PARP7 were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG, p300, and C/EBPβ. (F and G) Bar graphs showing the enrichment of (F) C/EBPβ or (G) p300 at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to siRNA-mediated depletion of Parp7 . Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; ** p < 0.01, *** p < 0.005, **** p < 0.001, and n.s., not significant. (H and I) Bar graphs showing the enrichment of (H) C/EBPβ and (I) H3K27ac at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to treatment with the p300 inhibitor A-485. Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; * p < 0.05, ** p < 0.01, and **** p < 0.001.
Histone H2bk5ac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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histone h2bk5ac - by Bioz Stars, 2026-05
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(A) Western blot showing a time course of PARP7 and C/EBPβ expression in 3T3-L1 cells differentiated with MDI cocktail. (B) Bar graphs showing the enrichment of C/EBPβ at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells with or without differentiation using MDI cocktail. Mean + SEM; n = 3. Asterisks indicate significant differences from control; ANOVA; * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001. (C) IP-western assays showing interactions between C/EBPβ and PARP7 in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-C/EBPβ were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG and PARP7. (D) Western blots of chromatin fractions from 3T3-L1 cells showing two p300-mediated histone modifications, H3K27ac and <t>H2BK5ac,</t> as well as H3K27me3, in bulk histones. PARP7 input confirms expected stabilization (2 nd lane from left) and knockdown (3 rd lane from left) of PARP7. (E) IP-western assays showing interactions between PARP7, p300, and C/EBPβ in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-PARP7 were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG, p300, and C/EBPβ. (F and G) Bar graphs showing the enrichment of (F) C/EBPβ or (G) p300 at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to siRNA-mediated depletion of Parp7 . Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; ** p < 0.01, *** p < 0.005, **** p < 0.001, and n.s., not significant. (H and I) Bar graphs showing the enrichment of (H) C/EBPβ and (I) H3K27ac at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to treatment with the p300 inhibitor A-485. Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; * p < 0.05, ** p < 0.01, and **** p < 0.001.
Acetyl Histone H2bk12, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acetyl histone h2bk12/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
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h2bk5ac  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc h2bk5ac
(A) Western blot showing a time course of PARP7 and C/EBPβ expression in 3T3-L1 cells differentiated with MDI cocktail. (B) Bar graphs showing the enrichment of C/EBPβ at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells with or without differentiation using MDI cocktail. Mean + SEM; n = 3. Asterisks indicate significant differences from control; ANOVA; * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001. (C) IP-western assays showing interactions between C/EBPβ and PARP7 in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-C/EBPβ were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG and PARP7. (D) Western blots of chromatin fractions from 3T3-L1 cells showing two p300-mediated histone modifications, H3K27ac and <t>H2BK5ac,</t> as well as H3K27me3, in bulk histones. PARP7 input confirms expected stabilization (2 nd lane from left) and knockdown (3 rd lane from left) of PARP7. (E) IP-western assays showing interactions between PARP7, p300, and C/EBPβ in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-PARP7 were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG, p300, and C/EBPβ. (F and G) Bar graphs showing the enrichment of (F) C/EBPβ or (G) p300 at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to siRNA-mediated depletion of Parp7 . Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; ** p < 0.01, *** p < 0.005, **** p < 0.001, and n.s., not significant. (H and I) Bar graphs showing the enrichment of (H) C/EBPβ and (I) H3K27ac at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to treatment with the p300 inhibitor A-485. Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; * p < 0.05, ** p < 0.01, and **** p < 0.001.
H2bk5ac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2bk5ac/product/Cell Signaling Technology Inc
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h2bk5ac - by Bioz Stars, 2026-05
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(A) Western blot showing a time course of PARP7 and C/EBPβ expression in 3T3-L1 cells differentiated with MDI cocktail. (B) Bar graphs showing the enrichment of C/EBPβ at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells with or without differentiation using MDI cocktail. Mean + SEM; n = 3. Asterisks indicate significant differences from control; ANOVA; * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001. (C) IP-western assays showing interactions between C/EBPβ and PARP7 in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-C/EBPβ were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG and PARP7. (D) Western blots of chromatin fractions from 3T3-L1 cells showing two p300-mediated histone modifications, H3K27ac and H2BK5ac, as well as H3K27me3, in bulk histones. PARP7 input confirms expected stabilization (2 nd lane from left) and knockdown (3 rd lane from left) of PARP7. (E) IP-western assays showing interactions between PARP7, p300, and C/EBPβ in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-PARP7 were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG, p300, and C/EBPβ. (F and G) Bar graphs showing the enrichment of (F) C/EBPβ or (G) p300 at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to siRNA-mediated depletion of Parp7 . Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; ** p < 0.01, *** p < 0.005, **** p < 0.001, and n.s., not significant. (H and I) Bar graphs showing the enrichment of (H) C/EBPβ and (I) H3K27ac at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to treatment with the p300 inhibitor A-485. Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; * p < 0.05, ** p < 0.01, and **** p < 0.001.

Journal: Cell reports

Article Title: NAD + sensing by PARP7 regulates the C/EBPβ-dependent transcription program during adipogenesis

doi: 10.1016/j.celrep.2026.116929

Figure Lengend Snippet: (A) Western blot showing a time course of PARP7 and C/EBPβ expression in 3T3-L1 cells differentiated with MDI cocktail. (B) Bar graphs showing the enrichment of C/EBPβ at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells with or without differentiation using MDI cocktail. Mean + SEM; n = 3. Asterisks indicate significant differences from control; ANOVA; * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001. (C) IP-western assays showing interactions between C/EBPβ and PARP7 in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-C/EBPβ were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG and PARP7. (D) Western blots of chromatin fractions from 3T3-L1 cells showing two p300-mediated histone modifications, H3K27ac and H2BK5ac, as well as H3K27me3, in bulk histones. PARP7 input confirms expected stabilization (2 nd lane from left) and knockdown (3 rd lane from left) of PARP7. (E) IP-western assays showing interactions between PARP7, p300, and C/EBPβ in 3T3-L1 cells. 3T3-L1 cells with Dox-inducible ectopic expression of FLAG-PARP7 were subjected to FLAG IP. The IPs were subjected to western blotting for FLAG, p300, and C/EBPβ. (F and G) Bar graphs showing the enrichment of (F) C/EBPβ or (G) p300 at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to siRNA-mediated depletion of Parp7 . Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; ** p < 0.01, *** p < 0.005, **** p < 0.001, and n.s., not significant. (H and I) Bar graphs showing the enrichment of (H) C/EBPβ and (I) H3K27ac at the promoters of target genes Pparg , Atf3 , and Klf15 , as assayed by ChIP-qPCR, in 3T3-L1 cells differentiated for 24 h using MDI cocktail and subjected to treatment with the p300 inhibitor A-485. Mean + SEM; n = 3. Asterisks indicate significant differences from control; Student’s t test; * p < 0.05, ** p < 0.01, and **** p < 0.001.

Article Snippet: Other antibodies used were as follows: PARP7 (Thermo Fisher Scientific, PA5–40774; RRID:AB_2607074), C/EBPβ (Invitrogen, PA5–120052; RRID:AB_2913624, Invitrogen, PA5–86117; RRID:AB_2802916, and Cell Signaling Technology, 3082; RRID:AB_2260365), Phospho-C/EBPβ (Thr235) (Cell Signaling Technology, 3084S; RRID:AB_2260359), PPARγ (81B8) (Cell Signaling Technology, 2443; RRID:AB_823598), cJun (Proteintech, 24909–1-AP; RRID:AB_2860574), cJun (Cell Signaling Technology, 9165; RRID:AB_2130165), DTX2 (ThermoFisher, PA5–109664; RRID:AB_2855075), RNF114 (Proteintech, 14338–1-AP; RRID:AB_3085435), Histone H3K27ac (Active Motif, 39134; RRID:AB_2722569), Histone H2BK5ac (Cell Signaling Technology, 12799; RRID:AB_2636805), Histone H3K27me3 (Cell Signaling Technology, 9733; RRID:AB_2616029), FLAG (Sigma-Aldrich, F3165; RRID:AB_259529), MAR binding reagent (Millipore, MABE1076; RRID:AB_2665469), PAR binding reagent (Millipore, MABE1031; RRID:AB_2665467), Perilipin (Biosynth, 20R-PP004; RRID: AB_3665667), Ubiquitin (E4I2J) (Cell Signaling Technology, 43124; RRID:AB_2799235), K48-linkage specific polyubiquitin (Cell Signaling Technology, 4289; RRID:AB_10557239), p300 (Active Motif, 61404; RRID:AB_2716754), p300 (Santa Cruz, SC-584; RRID:AB_2293429), NAMPT (D1K6D) (Cell Signaling Technology, 61122; RRID:AB_2799602), NAMPT (Proteintech, 11776–1-AP; RRID:AB_2298317), β-tubulin (Abcam, ab6046; RRID:AB_2210370), and β-Actin (8H10D10) (Cell Signaling Technology, 3700; RRID:AB_2242334).

Techniques: Western Blot, Expressing, ChIP-qPCR, Control, Knockdown