melan a melanocytes  (Qiagen)

 
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    Name:
    HiSpeed Plasmid Maxi Kit
    Description:
    For ultrafast purification of up to 750 µg transfection grade plasmid or cosmid DNA Kit contents Qiagen HiSpeed Plasmid Maxi Kit 10 preps 150L to 250mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 60 min Time Run 750g Yield For Ultrafast Purification of up to 750g Transfection grade Plasmid or Cosmid DNA Includes 10 HiSpeed Maxi Tips 10 QIAfilter Maxi Cartridges 10 QIAprecipitator Maxi Modules plus Syringes Reagents Buffers Benefits Less than 60 minutes prep time Lysate clearing and isopropanol precipitation without centrifugation No risk of DNA pellet loss during precipitation Up to 750 µg yield of high copy plasmid DNA LyseBlue for optimum lysis and maximum DNA yield Large scale plasmid preparation without vacuum manifo
    Catalog Number:
    12662
    Price:
    300
    Category:
    HiSpeed Plasmid Kits
    Buy from Supplier


    Structured Review

    Qiagen melan a melanocytes
    HiSpeed Plasmid Maxi Kit
    For ultrafast purification of up to 750 µg transfection grade plasmid or cosmid DNA Kit contents Qiagen HiSpeed Plasmid Maxi Kit 10 preps 150L to 250mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 60 min Time Run 750g Yield For Ultrafast Purification of up to 750g Transfection grade Plasmid or Cosmid DNA Includes 10 HiSpeed Maxi Tips 10 QIAfilter Maxi Cartridges 10 QIAprecipitator Maxi Modules plus Syringes Reagents Buffers Benefits Less than 60 minutes prep time Lysate clearing and isopropanol precipitation without centrifugation No risk of DNA pellet loss during precipitation Up to 750 µg yield of high copy plasmid DNA LyseBlue for optimum lysis and maximum DNA yield Large scale plasmid preparation without vacuum manifo
    https://www.bioz.com/result/melan a melanocytes/product/Qiagen
    Average 90 stars, based on 33895 article reviews
    Price from $9.99 to $1999.99
    melan a melanocytes - by Bioz Stars, 2020-08
    90/100 stars

    Images

    1) Product Images from "Rab27a Is an Essential Component of Melanosome Receptor for Myosin Va"

    Article Title: Rab27a Is an Essential Component of Melanosome Receptor for Myosin Va

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.01-12-0595

    All six myosin Va tail domain GFP fusions are stable proteins in vivo. Shown are Western blots of whole cell extracts prepared from melan-a melanocytes transfected with each of the six myosin Va tail domain GFP fusion protein constructs described in the text and probed with an antibody to GFP (lanes 1–6). The arrows indicate the positions of the fusion proteins. The remaining bands can be largely if not entirely accounted for by bands appearing in the blot of untransfected melan-a cells (lane 7). The hash marks to the left indicate the migration of the following markers (top to bottom): 200, 116, 97, 66, 55, 36, and 31 kDa. The molecular masses of all six fusion proteins calculated from these blots are within a few percentage points of their estimated molecular masses based on sequence (MC ST, 95 kDa; BR ST, 89 kDa; MC STΔF, 92 kDa; MC STΔD, 92 kDa; MC STK, 52 kDa; and GTD, 72 kDa). Moreover, all six proteins are largely if not entirely intact (only MC STK shows one stable breakdown product of ∼44 kDa). The differences in the intensities of the background bands in lanes 1–6 are due to differences in the amounts of whole cell extract loaded. The differences in the amount of fusion protein per volume of extract were due largely to differences in transfection efficiencies for the different plasmids.
    Figure Legend Snippet: All six myosin Va tail domain GFP fusions are stable proteins in vivo. Shown are Western blots of whole cell extracts prepared from melan-a melanocytes transfected with each of the six myosin Va tail domain GFP fusion protein constructs described in the text and probed with an antibody to GFP (lanes 1–6). The arrows indicate the positions of the fusion proteins. The remaining bands can be largely if not entirely accounted for by bands appearing in the blot of untransfected melan-a cells (lane 7). The hash marks to the left indicate the migration of the following markers (top to bottom): 200, 116, 97, 66, 55, 36, and 31 kDa. The molecular masses of all six fusion proteins calculated from these blots are within a few percentage points of their estimated molecular masses based on sequence (MC ST, 95 kDa; BR ST, 89 kDa; MC STΔF, 92 kDa; MC STΔD, 92 kDa; MC STK, 52 kDa; and GTD, 72 kDa). Moreover, all six proteins are largely if not entirely intact (only MC STK shows one stable breakdown product of ∼44 kDa). The differences in the intensities of the background bands in lanes 1–6 are due to differences in the amounts of whole cell extract loaded. The differences in the amount of fusion protein per volume of extract were due largely to differences in transfection efficiencies for the different plasmids.

    Techniques Used: In Vivo, Western Blot, Transfection, Construct, Migration, Sequencing

    MC ST, but not BR ST, colocalizes with melanosomes and generates a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC ST (A and B) or GFP-BR ST (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6.5 μm.
    Figure Legend Snippet: MC ST, but not BR ST, colocalizes with melanosomes and generates a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC ST (A and B) or GFP-BR ST (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6.5 μm.

    Techniques Used: Fluorescence, Transfection

    Neither MC STK, nor GTD, colocalizes with melanosomes or generate a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and the GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STK (A and B) or GFP-GTD (C and D). Bars, 9.5 μm (A and B) and 10.5 (C and D) μm.
    Figure Legend Snippet: Neither MC STK, nor GTD, colocalizes with melanosomes or generate a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and the GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STK (A and B) or GFP-GTD (C and D). Bars, 9.5 μm (A and B) and 10.5 (C and D) μm.

    Techniques Used: Fluorescence, Transfection

    MC STΔD, but not MC STΔF, colocalizes with melanosomes and generates a dominant negative phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STΔD (A and B) or GFP-MC STΔF (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6 μm.
    Figure Legend Snippet: MC STΔD, but not MC STΔF, colocalizes with melanosomes and generates a dominant negative phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STΔD (A and B) or GFP-MC STΔF (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6 μm.

    Techniques Used: Dominant Negative Mutation, Fluorescence, Transfection

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    Article Snippet: .. Plasmids for transfection were isolated from 250 ml cultures of Escherichia coli , XL10-Gold Ultracompetent Cells (Stratagene) by maxi-prep [using HiSpeed1Plasmid Maxi Kit (Qiagen 1)] to generate 50 μg of DNA used per transfection. .. Transfection with CRISPR/Cas9 constructs was performed using the “spontaneous uptake method” as previously described (Deitsch et al., ).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Amplification:

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Isolation:

    Article Title: Generation of Novel Plasmodium falciparum NF135 and NF54 Lines Expressing Fluorescent Reporter Proteins Under the Control of Strong and Constitutive Promoters
    Article Snippet: .. Plasmids for transfection were isolated from 250 ml cultures of Escherichia coli , XL10-Gold Ultracompetent Cells (Stratagene) by maxi-prep [using HiSpeed1Plasmid Maxi Kit (Qiagen 1)] to generate 50 μg of DNA used per transfection. .. Transfection with CRISPR/Cas9 constructs was performed using the “spontaneous uptake method” as previously described (Deitsch et al., ).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Construct:

    Article Title: Evaluation of chemotherapy with nanosomal paclitaxel and gene therapy expressing apoptosis-inducing proteins in the management of spontaneous canine mammary neoplasm
    Article Snippet: .. This gene construct was purified by QIAGEN HISPEED maxi column (catalogue no.12662). .. The purified constructs were used for oncolytic viral gene construct therapy.

    Purification:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: .. Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA). .. In vitro RNA transcription : T7 mMessage machine (Ambion, Austin, TX).

    Article Title: Evaluation of chemotherapy with nanosomal paclitaxel and gene therapy expressing apoptosis-inducing proteins in the management of spontaneous canine mammary neoplasm
    Article Snippet: .. This gene construct was purified by QIAGEN HISPEED maxi column (catalogue no.12662). .. The purified constructs were used for oncolytic viral gene construct therapy.

    Article Title: Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Article Snippet: .. Plasmids were purified using the HiSpeed Maxi Plasmid Kit (Qiagen, Valencia, CA). .. HeLa cells were washed twice with PBS and collected via centrifugation at 4°C.

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
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    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: .. Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen). .. The purified plasmid was digested with BstNI for run-off transcription as described previously ( ).

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: .. 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid DNA consisted of 97% supercoiled, 2% concatemeric, and 1% nicked circular forms.

    Article Title: Cisplatin Radiosensitization of DNA Irradiated with 2–20 eV Electrons: Role of Transient Anions
    Article Snippet: .. Plasmid DNA (pGEM-3Zf(−), 3197 bp) was extracted from E. coli DH5 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid DNA was eluted in buffer TE (10 mM Tris and 1 mM EDTA); it consisted of 97% supercoiled, 1% CL, and 2% concatemeric forms.

    Plasmid Preparation:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: .. Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA). .. In vitro RNA transcription : T7 mMessage machine (Ambion, Austin, TX).

    Article Title: Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Article Snippet: .. Plasmids were purified using the HiSpeed Maxi Plasmid Kit (Qiagen, Valencia, CA). .. HeLa cells were washed twice with PBS and collected via centrifugation at 4°C.

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: .. Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen). .. The purified plasmid was digested with BstNI for run-off transcription as described previously ( ).

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: .. 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid DNA consisted of 97% supercoiled, 2% concatemeric, and 1% nicked circular forms.

    Article Title: Cisplatin Radiosensitization of DNA Irradiated with 2–20 eV Electrons: Role of Transient Anions
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    Qiagen hispeed plasmid maxi kit
    Hispeed Plasmid Maxi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hispeed plasmid maxi kit/product/Qiagen
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    hispeed plasmid maxi kit - by Bioz Stars, 2020-08
    99/100 stars
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