melan a melanocytes  (Qiagen)

 
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    Name:
    HiSpeed Plasmid Maxi Kit
    Description:
    For ultrafast purification of up to 750 µg transfection grade plasmid or cosmid DNA Kit contents Qiagen HiSpeed Plasmid Maxi Kit 10 preps 150L to 250mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 60 min Time Run 750g Yield For Ultrafast Purification of up to 750g Transfection grade Plasmid or Cosmid DNA Includes 10 HiSpeed Maxi Tips 10 QIAfilter Maxi Cartridges 10 QIAprecipitator Maxi Modules plus Syringes Reagents Buffers Benefits Less than 60 minutes prep time Lysate clearing and isopropanol precipitation without centrifugation No risk of DNA pellet loss during precipitation Up to 750 µg yield of high copy plasmid DNA LyseBlue for optimum lysis and maximum DNA yield Large scale plasmid preparation without vacuum manifo
    Catalog Number:
    12662
    Price:
    300
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    HiSpeed Plasmid Kits
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    Structured Review

    Qiagen melan a melanocytes
    HiSpeed Plasmid Maxi Kit
    For ultrafast purification of up to 750 µg transfection grade plasmid or cosmid DNA Kit contents Qiagen HiSpeed Plasmid Maxi Kit 10 preps 150L to 250mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 60 min Time Run 750g Yield For Ultrafast Purification of up to 750g Transfection grade Plasmid or Cosmid DNA Includes 10 HiSpeed Maxi Tips 10 QIAfilter Maxi Cartridges 10 QIAprecipitator Maxi Modules plus Syringes Reagents Buffers Benefits Less than 60 minutes prep time Lysate clearing and isopropanol precipitation without centrifugation No risk of DNA pellet loss during precipitation Up to 750 µg yield of high copy plasmid DNA LyseBlue for optimum lysis and maximum DNA yield Large scale plasmid preparation without vacuum manifo
    https://www.bioz.com/result/melan a melanocytes/product/Qiagen
    Average 90 stars, based on 25612 article reviews
    Price from $9.99 to $1999.99
    melan a melanocytes - by Bioz Stars, 2020-05
    90/100 stars

    Images

    1) Product Images from "Rab27a Is an Essential Component of Melanosome Receptor for Myosin Va"

    Article Title: Rab27a Is an Essential Component of Melanosome Receptor for Myosin Va

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.01-12-0595

    All six myosin Va tail domain GFP fusions are stable proteins in vivo. Shown are Western blots of whole cell extracts prepared from melan-a melanocytes transfected with each of the six myosin Va tail domain GFP fusion protein constructs described in the text and probed with an antibody to GFP (lanes 1–6). The arrows indicate the positions of the fusion proteins. The remaining bands can be largely if not entirely accounted for by bands appearing in the blot of untransfected melan-a cells (lane 7). The hash marks to the left indicate the migration of the following markers (top to bottom): 200, 116, 97, 66, 55, 36, and 31 kDa. The molecular masses of all six fusion proteins calculated from these blots are within a few percentage points of their estimated molecular masses based on sequence (MC ST, 95 kDa; BR ST, 89 kDa; MC STΔF, 92 kDa; MC STΔD, 92 kDa; MC STK, 52 kDa; and GTD, 72 kDa). Moreover, all six proteins are largely if not entirely intact (only MC STK shows one stable breakdown product of ∼44 kDa). The differences in the intensities of the background bands in lanes 1–6 are due to differences in the amounts of whole cell extract loaded. The differences in the amount of fusion protein per volume of extract were due largely to differences in transfection efficiencies for the different plasmids.
    Figure Legend Snippet: All six myosin Va tail domain GFP fusions are stable proteins in vivo. Shown are Western blots of whole cell extracts prepared from melan-a melanocytes transfected with each of the six myosin Va tail domain GFP fusion protein constructs described in the text and probed with an antibody to GFP (lanes 1–6). The arrows indicate the positions of the fusion proteins. The remaining bands can be largely if not entirely accounted for by bands appearing in the blot of untransfected melan-a cells (lane 7). The hash marks to the left indicate the migration of the following markers (top to bottom): 200, 116, 97, 66, 55, 36, and 31 kDa. The molecular masses of all six fusion proteins calculated from these blots are within a few percentage points of their estimated molecular masses based on sequence (MC ST, 95 kDa; BR ST, 89 kDa; MC STΔF, 92 kDa; MC STΔD, 92 kDa; MC STK, 52 kDa; and GTD, 72 kDa). Moreover, all six proteins are largely if not entirely intact (only MC STK shows one stable breakdown product of ∼44 kDa). The differences in the intensities of the background bands in lanes 1–6 are due to differences in the amounts of whole cell extract loaded. The differences in the amount of fusion protein per volume of extract were due largely to differences in transfection efficiencies for the different plasmids.

    Techniques Used: In Vivo, Western Blot, Transfection, Construct, Migration, Sequencing

    MC ST, but not BR ST, colocalizes with melanosomes and generates a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC ST (A and B) or GFP-BR ST (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6.5 μm.
    Figure Legend Snippet: MC ST, but not BR ST, colocalizes with melanosomes and generates a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC ST (A and B) or GFP-BR ST (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6.5 μm.

    Techniques Used: Fluorescence, Transfection

    Neither MC STK, nor GTD, colocalizes with melanosomes or generate a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and the GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STK (A and B) or GFP-GTD (C and D). Bars, 9.5 μm (A and B) and 10.5 (C and D) μm.
    Figure Legend Snippet: Neither MC STK, nor GTD, colocalizes with melanosomes or generate a dilute-like phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and the GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STK (A and B) or GFP-GTD (C and D). Bars, 9.5 μm (A and B) and 10.5 (C and D) μm.

    Techniques Used: Fluorescence, Transfection

    MC STΔD, but not MC STΔF, colocalizes with melanosomes and generates a dominant negative phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STΔD (A and B) or GFP-MC STΔF (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6 μm.
    Figure Legend Snippet: MC STΔD, but not MC STΔF, colocalizes with melanosomes and generates a dominant negative phenotype in wild-type melanocytes. Shown is the distribution of melanosomes (A and C) and GFP fluorescence (B and D) in melan-a melanocytes transfected with either GFP-MC STΔD (A and B) or GFP-MC STΔF (C and D). The edges of the transfected cell in A are marked with a white line. Bars, 6 μm.

    Techniques Used: Dominant Negative Mutation, Fluorescence, Transfection

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    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
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    shRNA:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Construct:

    Article Title: Evaluation of chemotherapy with nanosomal paclitaxel and gene therapy expressing apoptosis-inducing proteins in the management of spontaneous canine mammary neoplasm
    Article Snippet: .. This gene construct was purified by QIAGEN HISPEED maxi column (catalogue no.12662). .. The purified constructs were used for oncolytic viral gene construct therapy.

    Purification:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: .. Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA). .. In vitro RNA transcription : T7 mMessage machine (Ambion, Austin, TX).

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. ..

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Article Title: Evaluation of chemotherapy with nanosomal paclitaxel and gene therapy expressing apoptosis-inducing proteins in the management of spontaneous canine mammary neoplasm
    Article Snippet: .. This gene construct was purified by QIAGEN HISPEED maxi column (catalogue no.12662). .. The purified constructs were used for oncolytic viral gene construct therapy.

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA). .. The non-targeting- and Beclin1-specific small interfering RNAs (siRNAs) were purchased from Dharmacon (Lafayette, CO) and cells were transfected using the nucleofection kit V specific for PC-3 transfection from Amaxa Biosystems (Gaithersburg, MD).

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
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    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
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    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA). .. Lipofectamine 2000 (Invitrogen) was used for transient transfection.

    Sequencing:

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection. .. Freshly isolated PBMCs or AutoMACS-purified monocytes were transfected with the use of the Nucleofector II (Lonza) as described by the manufacturer.

    Incubation:

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. ..

    Amplification:

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA). .. The non-targeting- and Beclin1-specific small interfering RNAs (siRNAs) were purchased from Dharmacon (Lafayette, CO) and cells were transfected using the nucleofection kit V specific for PC-3 transfection from Amaxa Biosystems (Gaithersburg, MD).

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    Polymerase Chain Reaction:

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    Article Snippet: .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. ..

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

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  • 98
    Qiagen hispeed plasmid maxi kit
    Hispeed Plasmid Maxi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hispeed plasmid maxi kit/product/Qiagen
    Average 98 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    hispeed plasmid maxi kit - by Bioz Stars, 2020-05
    98/100 stars
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