Journal: International Journal of Molecular Sciences

Article Title: Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

doi: 10.3390/ijms25053061

Figure Lengend Snippet: Comparison of Opa interacting protein 5 (OIP5) expression levels in colorectal tissues and correlation analysis of OIP5 and MISP expression. ( A ) Comparison of the expression levels of OIP5 in colorectal tissues between healthy individuals and CRC patients, analyzing primary tumors and adjacent normal tissues using data from TCGA. The analysis included normal tissues from healthy subjects ( n = 308) and CRC patients (primary tumors, n = 288; peritumoral normal tissues, n = 41). ** p < 0.01; **** p < 0.0001. ( B ) Correlation analysis was conducted to assess the relationship between MISP and OIP5 expression in colon tissues from healthy subjects. The dataset included normal tissues from healthy individuals ( n = 308).

Article Snippet: Rabbit polyclonal anti-MISP antibody (3 µL, 26338-1-AP, Proteintech) and rabbit polyclonal anti-OIP5 antibody (5 µL, 12142-1-AP, Proteintech) were used for immunoprecipitation.

Techniques: Comparison, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

doi: 10.3390/ijms25053061

Figure Lengend Snippet: Verification of the interaction between MISP and OIP5. ( A ) Immunofluorescence staining was conducted to determine the localization of MISP and OIP5 in HCT116 cells. Scale bars, 50 µm. ( B , C ) Schematic diagrams illustrating the construction of expression plasmids for ( B ) MISP and ( C ) OIP5. ( D – F ) Analysis of the formation of MISP and OIP5 complexes using immunoprecipitation (IP) with ( D ) MISP and ( E ) OIP5 antibodies. ( F ) The input samples. Arrowheads a and b indicate MISP, while arrowheads c and d indicate OIP5, with c being exogenous and d being endogenous.

Article Snippet: Rabbit polyclonal anti-MISP antibody (3 µL, 26338-1-AP, Proteintech) and rabbit polyclonal anti-OIP5 antibody (5 µL, 12142-1-AP, Proteintech) were used for immunoprecipitation.

Techniques: Immunofluorescence, Staining, Expressing, Immunoprecipitation

Journal: International Journal of Molecular Sciences

Article Title: Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

doi: 10.3390/ijms25053061

Figure Lengend Snippet: Interrelationship between the expression levels of MISP and OIP5. ( A , B ) Analysis of changes in OIP5 expression resulting from decreased MISP expression in ( A ) SW620 and ( B ) HCT116 cells. ( C , D ) Investigation of the impact of reduced OIP5 expression on MISP expression levels in ( C ) SW620 and ( D ) HCT116 cells. Each set of experiments was conducted with three replicates ( n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001; n.s., no significant difference.

Article Snippet: Rabbit polyclonal anti-MISP antibody (3 µL, 26338-1-AP, Proteintech) and rabbit polyclonal anti-OIP5 antibody (5 µL, 12142-1-AP, Proteintech) were used for immunoprecipitation.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

doi: 10.3390/ijms25053061

Figure Lengend Snippet: Effect of OIP5 on cell proliferation in human CRC cells. ( A , B ) Investigation of the effect of reduced OIP5 expression on cell proliferation in ( A ) SW620 and ( B ) HCT116 cells. ( C – F ) Assessment of the impact of OIP5 overexpression on cell proliferation under reduced MISP expression in ( C , D ) SW620 and ( E , F ) HCT116 cells. Each experiment was conducted with four replicates ( n = 4). # p < 0.1 (indicating a trend); ** p < 0.01; *** p < 0.001; **** p < 0.0001; n.s., no significant difference.

Article Snippet: Rabbit polyclonal anti-MISP antibody (3 µL, 26338-1-AP, Proteintech) and rabbit polyclonal anti-OIP5 antibody (5 µL, 12142-1-AP, Proteintech) were used for immunoprecipitation.

Techniques: Expressing, Over Expression

Journal: International Journal of Molecular Sciences

Article Title: Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway

doi: 10.3390/ijms25053061

Figure Lengend Snippet: Schematic diagram of signaling pathways involving MISP. MISP promotes colorectal tumorigenesis by upregulating phosphorylated STAT3 in the JAK2-STAT3 signaling pathway, in addition to its interaction with OIP5.

Article Snippet: Rabbit polyclonal anti-MISP antibody (3 µL, 26338-1-AP, Proteintech) and rabbit polyclonal anti-OIP5 antibody (5 µL, 12142-1-AP, Proteintech) were used for immunoprecipitation.

Techniques: