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Agilent technologies 1200 hplc system
<t>RP-HPLC</t> analysis of 25S rRNA nucleosides from rrp8–G209R and rrp8–G209A mutant cells. After sucrose gradient centrifugation, 60S subunits were collected with the Density Gradient Fractionation System (Teledyne Isco), and 25S rRNA was isolated. The 25S rRNA was digested with nuclease P1 and bacterial alkaline phosphatase (Sigma-Aldrich). Nucleosides from the mutants ( C and D ) together with the respective wild-type strain ( A ) were analysed by RP-HPLC on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent <t>1200</t> HPLC system. ( B ) Estimation of m 1 A peak areas in different rrp8 mutants. The m 1 A peak areas detected in the RP-HPLC analysis of the corresponding strain were estimated using the Agilent ChemStation software. The value for the wild-type was set to 100%.
1200 Hplc System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA"

Article Title: Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA

Journal: Nucleic Acids Research

doi: 10.1093/nar/gks1102

RP-HPLC analysis of 25S rRNA nucleosides from rrp8–G209R and rrp8–G209A mutant cells. After sucrose gradient centrifugation, 60S subunits were collected with the Density Gradient Fractionation System (Teledyne Isco), and 25S rRNA was isolated. The 25S rRNA was digested with nuclease P1 and bacterial alkaline phosphatase (Sigma-Aldrich). Nucleosides from the mutants ( C and D ) together with the respective wild-type strain ( A ) were analysed by RP-HPLC on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. ( B ) Estimation of m 1 A peak areas in different rrp8 mutants. The m 1 A peak areas detected in the RP-HPLC analysis of the corresponding strain were estimated using the Agilent ChemStation software. The value for the wild-type was set to 100%.
Figure Legend Snippet: RP-HPLC analysis of 25S rRNA nucleosides from rrp8–G209R and rrp8–G209A mutant cells. After sucrose gradient centrifugation, 60S subunits were collected with the Density Gradient Fractionation System (Teledyne Isco), and 25S rRNA was isolated. The 25S rRNA was digested with nuclease P1 and bacterial alkaline phosphatase (Sigma-Aldrich). Nucleosides from the mutants ( C and D ) together with the respective wild-type strain ( A ) were analysed by RP-HPLC on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. ( B ) Estimation of m 1 A peak areas in different rrp8 mutants. The m 1 A peak areas detected in the RP-HPLC analysis of the corresponding strain were estimated using the Agilent ChemStation software. The value for the wild-type was set to 100%.

Techniques Used: High Performance Liquid Chromatography, Mutagenesis, Gradient Centrifugation, Fractionation, Isolation, Software

RP-HPLC analysis of mung bean digested RNA fragments. Specific sequences of the 25S rRNA from wild-type ( A and C ) and the rrp8 -_ C mutant ( B and D ), corresponding to Helix 25.1 and Helix 65, respectively, were isolated by hybridization to complementary deoxyoligonucleotides (Oligo645 and Oligo2142) followed by mung bean digestion. RP-HPLC analysis with these fragments was then carried out on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. The following amounts of digested RNA were loaded: (A) 1.9 μg, (B) 1.3 μg, (C) 2.9 μg and (D) 1.8 μg.
Figure Legend Snippet: RP-HPLC analysis of mung bean digested RNA fragments. Specific sequences of the 25S rRNA from wild-type ( A and C ) and the rrp8 -_ C mutant ( B and D ), corresponding to Helix 25.1 and Helix 65, respectively, were isolated by hybridization to complementary deoxyoligonucleotides (Oligo645 and Oligo2142) followed by mung bean digestion. RP-HPLC analysis with these fragments was then carried out on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. The following amounts of digested RNA were loaded: (A) 1.9 μg, (B) 1.3 μg, (C) 2.9 μg and (D) 1.8 μg.

Techniques Used: High Performance Liquid Chromatography, Mutagenesis, Isolation, Hybridization

Loss of Rrp8 influences the amount of 25S rRNA m1A modification. After sucrose gradient centrifugation, 60S subunits were collected with the Density Gradient Fractionation System (Teledyne Isco), and 25S rRNA was isolated. The 25S rRNA was digested with nuclease P1 and bacterial alkaline phosphatase (Sigma-Aldrich). Nucleosides from the wild-type ( A ) and the rrp8 -_ C mutant ( B ) were analysed by RP-HPLC on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. ( C ) Localization of m 1 A modifications of 25S rRNA is shown in the secondary structure of Helix 25.1 and Helix 65 ( http://www.rna.icmb.utexas.edu/ ). ( D ) 3D structure of the corresponding helices was made using PyMol software (PyMOL Molecular Graphics System, Version 1.2r3pre, Schröinger, LLC) using the recent 80S ribosome structure (3U5D.pdb and 3U5E.pdb). RNA is coloured in green, whereas the modified bases are shown in red spheres.
Figure Legend Snippet: Loss of Rrp8 influences the amount of 25S rRNA m1A modification. After sucrose gradient centrifugation, 60S subunits were collected with the Density Gradient Fractionation System (Teledyne Isco), and 25S rRNA was isolated. The 25S rRNA was digested with nuclease P1 and bacterial alkaline phosphatase (Sigma-Aldrich). Nucleosides from the wild-type ( A ) and the rrp8 -_ C mutant ( B ) were analysed by RP-HPLC on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 μm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. ( C ) Localization of m 1 A modifications of 25S rRNA is shown in the secondary structure of Helix 25.1 and Helix 65 ( http://www.rna.icmb.utexas.edu/ ). ( D ) 3D structure of the corresponding helices was made using PyMol software (PyMOL Molecular Graphics System, Version 1.2r3pre, Schröinger, LLC) using the recent 80S ribosome structure (3U5D.pdb and 3U5E.pdb). RNA is coloured in green, whereas the modified bases are shown in red spheres.

Techniques Used: Modification, Gradient Centrifugation, Fractionation, Isolation, Mutagenesis, High Performance Liquid Chromatography, Software

2) Product Images from "Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses"

Article Title: Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2014/580745

HPLC and TIC of typical TSC sample obtained using an Agilent 6130 Quadrupole LC-MS connected to an Agilent 1200 HPLC system. (a) HPLC (276 nm), (b) (+) TIC, and (c) (−) TIC.
Figure Legend Snippet: HPLC and TIC of typical TSC sample obtained using an Agilent 6130 Quadrupole LC-MS connected to an Agilent 1200 HPLC system. (a) HPLC (276 nm), (b) (+) TIC, and (c) (−) TIC.

Techniques Used: High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy

3) Product Images from "A New Class of Quorum Quenching Molecules from Staphylococcus Species Affects Communication and Growth of Gram-Negative Bacteria"

Article Title: A New Class of Quorum Quenching Molecules from Staphylococcus Species Affects Communication and Growth of Gram-Negative Bacteria

Journal: PLoS Pathogens

doi: 10.1371/journal.ppat.1003654

RP-HPLC profile, UV-spectrum and structures of the two QS-inhibitors purified from S. delphini . ( A ) QS-inhibitor, N -[2-(1H-indol-3-yl)ethyl]-urea (yayurea A). ( B ) QS-inhibitor, N -(2-phenethyl)-urea (yayurea B). RP-HPLC was carried out on an Agilent 1200 and Waters xBridge C18, 5 mm, 4.6×150 mm column; compounds were eluted with a 15 min linear gradient of 0.1% phosphoric acid to acetonitrile at a flow rate of 1.5 ml/min.
Figure Legend Snippet: RP-HPLC profile, UV-spectrum and structures of the two QS-inhibitors purified from S. delphini . ( A ) QS-inhibitor, N -[2-(1H-indol-3-yl)ethyl]-urea (yayurea A). ( B ) QS-inhibitor, N -(2-phenethyl)-urea (yayurea B). RP-HPLC was carried out on an Agilent 1200 and Waters xBridge C18, 5 mm, 4.6×150 mm column; compounds were eluted with a 15 min linear gradient of 0.1% phosphoric acid to acetonitrile at a flow rate of 1.5 ml/min.

Techniques Used: High Performance Liquid Chromatography, Purification, Flow Cytometry

4) Product Images from "Structural and functional characterization of the trifunctional antibody catumaxomab"

Article Title: Structural and functional characterization of the trifunctional antibody catumaxomab

Journal: mAbs

doi:

The catumaxomab sample was analyzed using RP-HPLC mass spectrometry using a PLRP-S column (Varian) with an acetonitrile gradient from 27% to 42% over 40 minutes on a 1200 HPLC system (Agilent) for separation, coupled with an Agilent 6220 ESI-TOF mass
Figure Legend Snippet: The catumaxomab sample was analyzed using RP-HPLC mass spectrometry using a PLRP-S column (Varian) with an acetonitrile gradient from 27% to 42% over 40 minutes on a 1200 HPLC system (Agilent) for separation, coupled with an Agilent 6220 ESI-TOF mass

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

5) Product Images from "KIOM-79 Protects AGE-Induced Retinal Pericyte Apoptosis via Inhibition of NF-kappaB Activation In Vitro and In Vivo"

Article Title: KIOM-79 Protects AGE-Induced Retinal Pericyte Apoptosis via Inhibition of NF-kappaB Activation In Vitro and In Vivo

Journal: PLoS ONE

doi: 10.1371/journal.pone.0043591

Analysis of KIOM-79 by HPLC. HPLC chromatogram of KIOM-79 by Agilent 1200 HPLC system with MWD detection at 254 nm (A) and 3D-HPLC chromatogram of KIOM-79 by Shimadzu HPLC system with DAD detection at 200–400 nm (B).
Figure Legend Snippet: Analysis of KIOM-79 by HPLC. HPLC chromatogram of KIOM-79 by Agilent 1200 HPLC system with MWD detection at 254 nm (A) and 3D-HPLC chromatogram of KIOM-79 by Shimadzu HPLC system with DAD detection at 200–400 nm (B).

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses"

Article Title: Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2014/580745

HPLC and TIC of typical TSC sample obtained using an Agilent 6130 Quadrupole LC-MS connected to an Agilent 1200 HPLC system. (a) HPLC (276 nm), (b) (+) TIC, and (c) (−) TIC.
Figure Legend Snippet: HPLC and TIC of typical TSC sample obtained using an Agilent 6130 Quadrupole LC-MS connected to an Agilent 1200 HPLC system. (a) HPLC (276 nm), (b) (+) TIC, and (c) (−) TIC.

Techniques Used: High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy

Related Articles

High Performance Liquid Chromatography:

Article Title: Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses
Article Snippet: .. LC-MS analyses were performed using an Agilent 6130 Quadrupole LC-MS (Agilent, Waldbronn, Germany) connected to an Agilent 1200 HPLC system (Agilent, Waldbronn, Germany). .. The parameters for MS analysis in the positive and negative ion mode were as follows: nebulizer, 35 psi; ionization voltage, 3500 V; dry temperature, 350°C; flow rate of carrier gas, 9.0 L·min−1 .

Article Title: KIOM-79 Protects AGE-Induced Retinal Pericyte Apoptosis via Inhibition of NF-kappaB Activation In Vitro and In Vivo
Article Snippet: .. HPLC Analysis of KIOM-79 To quantify major compounds of KIOM-79, the high-performance liquid chromatography (HPLC) analysis was performed by an Agilent 1200 HPLC system and the 3D-HPLC chromatogram was acquired by a Shimadzu HPLC system. .. Spherex C-18 analytical column (250×4.6 mm, 5.0 µ m, Phenomenex) was used with the mobile phase consisted of acetonitrile (A ) and 0.1% acetic acid in water (B ).

Article Title: Structural and functional characterization of the trifunctional antibody catumaxomab
Article Snippet: .. The peptides were separated on a reversed-phase HPLC using an Agilent 1200 HPLC system equipped with a diode-array detector, an auto-sampler, and a temperature controlled column compartment (Agilent, Waldbronn, Germany). .. The eluents were A: 0.1% TFA in water (Merck, Darmstadt, Germany) and B: 0.1% TFA in acetonitrile (Merck, Darmstadt, Germany).

Article Title: Enhanced absorption of hydroxysafflor yellow A using a self-double-emulsifying drug delivery system: in vitro and in vivo studies
Article Snippet: .. Samples were determined using an Agilent 1200 HPLC system with ZORBAX SB-C18 (4.6 mm × 250 mm, 5 μm) (Agilent, Santa Clara, CA). ..

Article Title: Biodegradable hybrid polymer micelles for combination drug therapy in ovarian cancer
Article Snippet: .. PTX levels were determined by high-performance liquid chromatography (HPLC) analysis under isocratic conditions using an Agilent 1200 HPLC system a diode array detector set at 227 nm. .. As stationary phase a Nucleosil C18 column was used (250 mm × 4.6 mm), a mobile phase of acetonitrile/water mixture (55/45, v/v) was applied at a flow rate of 1 mL/min.

Article Title: Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA
Article Snippet: .. Nucleosides were analysed by RP-HPLC on a Supelcosil LC-18-S HPLC column (25 cm × 4.6 mm, 5 µm) equipped with a pre-column (4.6 × 20 mm) at 30°C on an Agilent 1200 HPLC system. ..

Article Title: Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses
Article Snippet: .. LC-DAD-ESI-IT-MSn Analysis of Typical TSC Sample To comprehensively identify the chemical constituents in TSC sample by the fragmentation rules, a LC-DAD-ESI-IT-MSn experiment was performed using an Agilent 6320 ion-trap spectrometer (Agilent, Waldbronn, Germany) connected to an Agilent 1200 HPLC system (Agilent, Waldbronn, Germany). ..

Article Title: A New Class of Quorum Quenching Molecules from Staphylococcus Species Affects Communication and Growth of Gram-Negative Bacteria
Article Snippet: .. Qualitative analysis was carried out on an Agilent 1200 HPLC system (Agilent technologies, Waldbronn, Germany) and a RP-HPLC Waters xBridge C18, 5 mm, 4.6×150 mm column. ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses
Article Snippet: .. LC-MS analyses were performed using an Agilent 6130 Quadrupole LC-MS (Agilent, Waldbronn, Germany) connected to an Agilent 1200 HPLC system (Agilent, Waldbronn, Germany). .. The parameters for MS analysis in the positive and negative ion mode were as follows: nebulizer, 35 psi; ionization voltage, 3500 V; dry temperature, 350°C; flow rate of carrier gas, 9.0 L·min−1 .

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    Agilent technologies agilent 1200 hplc
    Performance comparison between EO-pumped <t>HPLC</t> and <t>Agilent</t> 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).
    Agilent 1200 Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 1200 hplc/product/Agilent technologies
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    agilent 1200 hplc - by Bioz Stars, 2020-07
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    88
    Agilent technologies gel permeation hplc equipment
    <t>GP-HPLC</t> of <t>PBLPS</t> from the four varieties of bamboo leave: PBLPS: Purified BLPS. ( a,b ) from Phyllostachys nigra (PN); ( c,d ) from Phyllostachys vivax (PV); ( e,f ) from Chimonobambusa quadrangularis (Fenzi) Makino (CQ); ( g,h ) from Phyllostachys bambusoides (PB).
    Gel Permeation Hplc Equipment, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies diode array detector hplc dad
    <t>HPLC-DAD</t> (high pressure liquid chromatography-diode-array detector) chromatograms of compounds in control (non-extruded shell) and milled shells extruded at 70 °C and 150 rpm with detection at 280 nm. Blue: control. Red: treatment 70 °C and 150 rpm. Retention time (t R , min).
    Diode Array Detector Hplc Dad, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies hplc dad
    <t>HPLC-DAD</t> chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3- O - α -rhamnopyranosyl-(1→2)[ α -rhamnopyran-osyl-(1→6)]- β -D-galactopyranoside, t R = 20.600 min), C3 (kaempferol 3- O -(6′′- O - E - p -coumaryl)- β -D-glucopyranoside, t R = 38.763 min).
    Hplc Dad, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Performance comparison between EO-pumped HPLC and Agilent 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).

    Journal: Analytical Chemistry

    Article Title: High-Pressure Open-Channel On-Chip Electroosmotic Pump for Nanoflow High Performance Liquid Chromatography

    doi: 10.1021/ac4040345

    Figure Lengend Snippet: Performance comparison between EO-pumped HPLC and Agilent 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).

    Article Snippet: Figure b,c presents a performance comparison between chromatograms from the EO-pumped HPLC (Figure b) and those from an Agilent 1200 HPLC (Figure c).

    Techniques: High Performance Liquid Chromatography, Chromatin Immunoprecipitation, Injection, Concentration Assay, Flow Cytometry

    GP-HPLC of PBLPS from the four varieties of bamboo leave: PBLPS: Purified BLPS. ( a,b ) from Phyllostachys nigra (PN); ( c,d ) from Phyllostachys vivax (PV); ( e,f ) from Chimonobambusa quadrangularis (Fenzi) Makino (CQ); ( g,h ) from Phyllostachys bambusoides (PB).

    Journal: Molecules

    Article Title: Chemical Constituents and Structural Characterization of Polysaccharides from Four Typical Bamboo Species Leaves

    doi: 10.3390/molecules20034162

    Figure Lengend Snippet: GP-HPLC of PBLPS from the four varieties of bamboo leave: PBLPS: Purified BLPS. ( a,b ) from Phyllostachys nigra (PN); ( c,d ) from Phyllostachys vivax (PV); ( e,f ) from Chimonobambusa quadrangularis (Fenzi) Makino (CQ); ( g,h ) from Phyllostachys bambusoides (PB).

    Article Snippet: The molecular weight of PBLPS-1 and PBLPS-2 was determined using gel permeation HPLC equipment (Agilent 1200) equipped with a detector of ELSD 3300/2000 and a TSK-gel G5000 column (7.8 mm × 30 cm, Tosoh (Shanghai)Co. Ltd., Shanghai, China).

    Techniques: High Performance Liquid Chromatography, Purification

    HPLC-DAD (high pressure liquid chromatography-diode-array detector) chromatograms of compounds in control (non-extruded shell) and milled shells extruded at 70 °C and 150 rpm with detection at 280 nm. Blue: control. Red: treatment 70 °C and 150 rpm. Retention time (t R , min).

    Journal: Biomolecules

    Article Title: In Vitro Antioxidant Activity Optimization of Nut Shell (Carya illinoinensis) by Extrusion Using Response Surface Methods

    doi: 10.3390/biom9120883

    Figure Lengend Snippet: HPLC-DAD (high pressure liquid chromatography-diode-array detector) chromatograms of compounds in control (non-extruded shell) and milled shells extruded at 70 °C and 150 rpm with detection at 280 nm. Blue: control. Red: treatment 70 °C and 150 rpm. Retention time (t R , min).

    Article Snippet: The compounds were quantified by high-performance liquid chromatography with a diode-array detector (HPLC–DAD) (1200 Series, Agilent Technologies, Santa Clara, CA, USA) using a reverse-phase column (Zorbax SB-Aq, Santa Clara, CA, USA) 4.6 mm ID × 150 mm (3.5 µm) scanning at different wavelengths of 254, 280, and 320 (obtained from an sample scanning).

    Techniques: High Performance Liquid Chromatography

    HPLC-DAD chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3- O - α -rhamnopyranosyl-(1→2)[ α -rhamnopyran-osyl-(1→6)]- β -D-galactopyranoside, t R = 20.600 min), C3 (kaempferol 3- O -(6′′- O - E - p -coumaryl)- β -D-glucopyranoside, t R = 38.763 min).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: In Vivo Evaluation of the Antiasthmatic, Antitussive, and Expectorant Activities and Chemical Components of Three Elaeagnus Leaves

    doi: 10.1155/2015/428208

    Figure Lengend Snippet: HPLC-DAD chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3- O - α -rhamnopyranosyl-(1→2)[ α -rhamnopyran-osyl-(1→6)]- β -D-galactopyranoside, t R = 20.600 min), C3 (kaempferol 3- O -(6′′- O - E - p -coumaryl)- β -D-glucopyranoside, t R = 38.763 min).

    Article Snippet: Chemical Analysis by HPLC/DAD About 1.0 g of three Elaeagnus leaves was immersed in water and decocted for 1 h. The extracted solution was filtered for the tested sample and detected by HPLC-DAD (Model 1200, Agilent Technologies Co. Ltd., USA).

    Techniques: High Performance Liquid Chromatography

    HPLC-DAD chromatographies of E. lanceolata leaf and monomeric compounds C3.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: In Vivo Evaluation of the Antiasthmatic, Antitussive, and Expectorant Activities and Chemical Components of Three Elaeagnus Leaves

    doi: 10.1155/2015/428208

    Figure Lengend Snippet: HPLC-DAD chromatographies of E. lanceolata leaf and monomeric compounds C3.

    Article Snippet: Chemical Analysis by HPLC/DAD About 1.0 g of three Elaeagnus leaves was immersed in water and decocted for 1 h. The extracted solution was filtered for the tested sample and detected by HPLC-DAD (Model 1200, Agilent Technologies Co. Ltd., USA).

    Techniques: High Performance Liquid Chromatography

    HPLC-DAD chromatographies of E. henryi leaf and monomeric compounds C1, C2 (kaempferol 3- O - α -L-rhamnopyranosyl-(1→2)- β -D-glucopyranoside, t R = 23.100 min), and C3.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: In Vivo Evaluation of the Antiasthmatic, Antitussive, and Expectorant Activities and Chemical Components of Three Elaeagnus Leaves

    doi: 10.1155/2015/428208

    Figure Lengend Snippet: HPLC-DAD chromatographies of E. henryi leaf and monomeric compounds C1, C2 (kaempferol 3- O - α -L-rhamnopyranosyl-(1→2)- β -D-glucopyranoside, t R = 23.100 min), and C3.

    Article Snippet: Chemical Analysis by HPLC/DAD About 1.0 g of three Elaeagnus leaves was immersed in water and decocted for 1 h. The extracted solution was filtered for the tested sample and detected by HPLC-DAD (Model 1200, Agilent Technologies Co. Ltd., USA).

    Techniques: High Performance Liquid Chromatography