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Promega 120 bp fragment
120 Bp Fragment, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 87 stars, based on 1 article reviews
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120 bp fragment - by Bioz Stars, 2020-01
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Clone Assay:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ). .. Positive cosmid genomic clones were analyzed and sequenced, and respective cDNA was obtained by RT-PCR using specific primers designed based on the genomic sequence.

Selection:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: A 120-bp PCR fragment of the C. parasitica adaptor protein 1μ subunit ( AP-1 μ), a coated vesicle adaptor that facilitates cargo selection in the late secretory trans -Golgi network (TGN) and in endosomes , was obtained from cDNA prepared from RNA obtained as previously described , using primers designed based on the Neurospora crassa sequence ( ): AP50asp50F (5′-TTAATGTCAAATTTGAGATTCCCTAT-3′) and AP50asp160Rev (5′-GACTGCGTGATGTACCGGACCC). .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ).

Polymerase Chain Reaction:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ). .. Positive cosmid genomic clones were analyzed and sequenced, and respective cDNA was obtained by RT-PCR using specific primers designed based on the genomic sequence.

Genomic Sequencing:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: Kex2 cDNA sequence was assembled from cDNA clones obtained from reverse transcription-PCR (RT-PCR) with specific primers derived from the genomic sequences. .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ).

Chromosome Walking:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: The compilation of the three PCR fragments derived from these primers allowed the design of two specific primers (60R, 5′-GGCGATGTTGGAGTCGT-3′, and 970R, 5′-GGACAATGAGGCCTC-3′) to start chromosome walking in both directions. .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ).

Sequencing:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: A 120-bp PCR fragment of the C. parasitica adaptor protein 1μ subunit ( AP-1 μ), a coated vesicle adaptor that facilitates cargo selection in the late secretory trans -Golgi network (TGN) and in endosomes , was obtained from cDNA prepared from RNA obtained as previously described , using primers designed based on the Neurospora crassa sequence ( ): AP50asp50F (5′-TTAATGTCAAATTTGAGATTCCCTAT-3′) and AP50asp160Rev (5′-GACTGCGTGATGTACCGGACCC). .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ).

Plasmid Preparation:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ). .. Positive cosmid genomic clones were analyzed and sequenced, and respective cDNA was obtained by RT-PCR using specific primers designed based on the genomic sequence.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: Kex2 cDNA sequence was assembled from cDNA clones obtained from reverse transcription-PCR (RT-PCR) with specific primers derived from the genomic sequences. .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ).

Derivative Assay:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: Kex2 cDNA sequence was assembled from cDNA clones obtained from reverse transcription-PCR (RT-PCR) with specific primers derived from the genomic sequences. .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ).

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  • 78
    Promega murine ccn2 ctgf cdna
    Effects of <t>rCCN2/CTGF</t> on ALPase activity from MPL cells. When MPL cells reached confluence, the culture medium was replaced with α-MEM containing 1% FBS, and the cells were then cultured with various concentrations of rCCN2/CTGF. After 48 hrs, the cell layers were collected, and ALPase activity was determined as described in
    Murine Ccn2 Ctgf Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine ccn2 ctgf cdna/product/Promega
    Average 78 stars, based on 1 article reviews
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    85
    Promega bst eii
    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with <t>Bst</t> <t>EII</t> (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.
    Bst Eii, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega kb m mitf promoter fragment
    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with <t>Bst</t> <t>EII</t> (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.
    Kb M Mitf Promoter Fragment, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega leishmania kdna minicircle
    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with <t>Bst</t> <t>EII</t> (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.
    Leishmania Kdna Minicircle, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    leishmania kdna minicircle - by Bioz Stars, 2020-01
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    Effects of rCCN2/CTGF on ALPase activity from MPL cells. When MPL cells reached confluence, the culture medium was replaced with α-MEM containing 1% FBS, and the cells were then cultured with various concentrations of rCCN2/CTGF. After 48 hrs, the cell layers were collected, and ALPase activity was determined as described in

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Effects of rCCN2/CTGF on ALPase activity from MPL cells. When MPL cells reached confluence, the culture medium was replaced with α-MEM containing 1% FBS, and the cells were then cultured with various concentrations of rCCN2/CTGF. After 48 hrs, the cell layers were collected, and ALPase activity was determined as described in "Materials and Methods." Values represent the averages ± SD of 3 separate experiments. Asterisks denote statistically significant differences from the vehicle-treated control at the significance level of * p

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Activity Assay, Cell Culture

    RT-PCR analysis of the expression of CCN2/CTGF mRNA in sparse (S) and confluent (C) MPL cultures. Total RNA was purified from MPL cultures under sparse and confluent conditions, and 1 μg in each sample was reverse transcribed and used for PCR. The expression of CCN2/CTGF mRNA was stronger under the sparse than the confluent condition as evaluated in the exponential phase of the amplification (25 cycles). Levels of GAPDH mRNA, used as an internal control, were almost the same among these samples.

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: RT-PCR analysis of the expression of CCN2/CTGF mRNA in sparse (S) and confluent (C) MPL cultures. Total RNA was purified from MPL cultures under sparse and confluent conditions, and 1 μg in each sample was reverse transcribed and used for PCR. The expression of CCN2/CTGF mRNA was stronger under the sparse than the confluent condition as evaluated in the exponential phase of the amplification (25 cycles). Levels of GAPDH mRNA, used as an internal control, were almost the same among these samples.

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Purification, Polymerase Chain Reaction, Amplification

    (A) Effect of rCCN2/CTGF on DNA synthesis in MPL cells. MPL cells were inoculated at a density of 5 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 2 days. Then, the culture medium was changed to α-MEM with 0.5% FBS, and the cells were incubated with various concentrations of rCCN2/CTGF for 24 hrs and labeled with [ 3 H] thymidine for the last 4 hrs. The incorporated radioactivity was determined by a liquid scintillation counter. Values represent the averages ± SD. Data were computed with the results of 2 independent series of experiments with multiple sample numbers. Asterisks denote statistically significant difference from the vehicle-treated control. (B) Effect of rCCN2/CTGF on the proliferation of MPL cells. MPL cells were inoculated at a density of 2 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 24 hrs. Then, the medium was changed to α-MEM with 1% FBS, and the cells were cultured with various concentrations of rCCN2/CTGF for 3 days. The cell number was computed by using Tetra-Color One as specified by the manufacturer. Points and bars represent the averages and SD, respectively. Asterisks indicate significant differences between vehicle-treated control and rCCN2/CTGF-treated samples at the significance level of ** p

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: (A) Effect of rCCN2/CTGF on DNA synthesis in MPL cells. MPL cells were inoculated at a density of 5 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 2 days. Then, the culture medium was changed to α-MEM with 0.5% FBS, and the cells were incubated with various concentrations of rCCN2/CTGF for 24 hrs and labeled with [ 3 H] thymidine for the last 4 hrs. The incorporated radioactivity was determined by a liquid scintillation counter. Values represent the averages ± SD. Data were computed with the results of 2 independent series of experiments with multiple sample numbers. Asterisks denote statistically significant difference from the vehicle-treated control. (B) Effect of rCCN2/CTGF on the proliferation of MPL cells. MPL cells were inoculated at a density of 2 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 24 hrs. Then, the medium was changed to α-MEM with 1% FBS, and the cells were cultured with various concentrations of rCCN2/CTGF for 3 days. The cell number was computed by using Tetra-Color One as specified by the manufacturer. Points and bars represent the averages and SD, respectively. Asterisks indicate significant differences between vehicle-treated control and rCCN2/CTGF-treated samples at the significance level of ** p

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: DNA Synthesis, Cell Culture, Incubation, Labeling, Radioactivity

    Effects of rCCN2/CTGF on mRNA expression of osteoblast and PDL-related markers in MPL cells. Confluent cultures of MPL cells were treated with rCCN2/CTGF (100 ng/ml, closed bars) or vehicle (dotted bars) for 24 hrs. Total RNA was purified, and 1 μg of each sample was reverse-transcribed and used for PCR. Alkaline phosphatase (ALPase), type I collagen, and periostin mRNAs were shown to be up-regulated clearly by the addition of rCCN2/CTGF under exponential conditions (25, 30, and 25 cycles, respectively) for amplification. The expression of GAPDH, used for an internal control, was almost the same among these samples.

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Effects of rCCN2/CTGF on mRNA expression of osteoblast and PDL-related markers in MPL cells. Confluent cultures of MPL cells were treated with rCCN2/CTGF (100 ng/ml, closed bars) or vehicle (dotted bars) for 24 hrs. Total RNA was purified, and 1 μg of each sample was reverse-transcribed and used for PCR. Alkaline phosphatase (ALPase), type I collagen, and periostin mRNAs were shown to be up-regulated clearly by the addition of rCCN2/CTGF under exponential conditions (25, 30, and 25 cycles, respectively) for amplification. The expression of GAPDH, used for an internal control, was almost the same among these samples.

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Expressing, Purification, Polymerase Chain Reaction, Amplification

    Effect of rCCN2/CTGF on the synthesis of collagen and total protein in MPL cells. Various concentrations of rCCN2/CTGF were added to confluent cultures of MPL cells, and the cells were cultured for 12 hrs. Then, the cells were labeled with [ 3 H] proline for another 12 hrs, after which their cell layers were collected for analysis. The radioactivities of [ 3 H] proline incorporated into total nascent proteins and collagenase-digestable portions were measured as described in

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Effect of rCCN2/CTGF on the synthesis of collagen and total protein in MPL cells. Various concentrations of rCCN2/CTGF were added to confluent cultures of MPL cells, and the cells were cultured for 12 hrs. Then, the cells were labeled with [ 3 H] proline for another 12 hrs, after which their cell layers were collected for analysis. The radioactivities of [ 3 H] proline incorporated into total nascent proteins and collagenase-digestable portions were measured as described in "Materials and Methods." Slashed and closed boxes indicate the collagen and total protein synthesis, respectively. Values represent the mean average ± SD (n = 2). Asterisks denote statistically significant differences (** p

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Cell Culture, Labeling

    Expression of CCN2/CTGF mRNA in sparse and confluent MPL cultures (in situ hybridization) with or without TGF-β stimulation. MPL cultures in the sparsity phase (A and B) and confluence phase (C and D) were treated with TGF-β (B and D) or PBS (control: A and C). The cells were then cultured for 12 hrs, fixed, and hybridized with an antisense riboprobe for ccn2/ctgf mRNA. CCN2/CTGF mRNA was distinctly expressed in the cells in the sparse (A), but not in those in the confluent (C) state. However, the addition of TGF-β enhanced the level of CCN2/CTGF mRNA strongly, not only in the sparse cultures (B), but also in the confluent ones (D).

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Expression of CCN2/CTGF mRNA in sparse and confluent MPL cultures (in situ hybridization) with or without TGF-β stimulation. MPL cultures in the sparsity phase (A and B) and confluence phase (C and D) were treated with TGF-β (B and D) or PBS (control: A and C). The cells were then cultured for 12 hrs, fixed, and hybridized with an antisense riboprobe for ccn2/ctgf mRNA. CCN2/CTGF mRNA was distinctly expressed in the cells in the sparse (A), but not in those in the confluent (C) state. However, the addition of TGF-β enhanced the level of CCN2/CTGF mRNA strongly, not only in the sparse cultures (B), but also in the confluent ones (D).

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Expressing, In Situ Hybridization, Cell Culture

    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with Bst EII (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Differentiation of "Mycobacterium canettii" from Other Mycobacterium tuberculosis Complex Organisms by PCR-Restriction Analysis of the hsp65 Gene

    doi: 10.1128/JCM.39.10.3705-3708.2001

    Figure Lengend Snippet: PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with Bst EII (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.

    Article Snippet: Although the results were similar for all 102 isolates studied after the digestions with Bst EII (three fragments of 235, 120, and 80 bp; Fig. A), Hae III (four fragments of 155, 130, 70, and 40 bp; Fig. B), and Aci I (three fragments of 185, 130, 90 bp; Fig. C), the digestion of the 441-bp PCR product with Hha I clearly differentiated “ M. canettii ” from all other isolates at the subspecies level (Fig. D); it resulted in three fragments of 260, 105, and 60 bp for “ M. canettii ,” whereas it resulted in four fragments of 185, 105, 75, and 60 bp for M. tuberculosis , M. bovis , and M. bovis BCG.

    Techniques: Polymerase Chain Reaction, Sequencing, Negative Control, Mutagenesis