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Promega 120 bp fragment
120 Bp Fragment, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/120 bp fragment/product/Promega
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
120 bp fragment - by Bioz Stars, 2020-08
88/100 stars

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Countercurrent Chromatography:

Article Title: Leishmania infection in blood donors: A new challenge in leishmaniasis transmission?
Article Snippet: .. Primers HM1 ( 5'-CCG CCC CTA TTT TAC ACC AAC CCC-3' ), HM2 ( 5'-GGG GAG GGG CGT TCT GCG AA-3' ), and HM3 ( 5'-GGC CCA CTA TAT TAC ACC AAC CCC-3' ) were employed to amplify the 120-bp fragment of the conserved region of Leishmania kDNA minicircle [ ]. kDNA-PCR was performed with a final 25 μL volume containing 1x Colorless GoTaq Flexi buffer (Promega, Madison, USA), 200 μM dNTPs (dATP, dCTP, dGTP, and dTTP; Promega), 1.5 mM MgCl2 , a 1 μM concentration of each primer , roughly 150 ng of extracted DNA, and water to complete the reaction. .. In all reactions, 2 μL of Leishmania ( Viannia ) braziliensis genomic DNA (MHOM/BR/1975/M2903, LIPMed-Fiocruz, Rio de Janeiro) served as the positive control.

Concentration Assay:

Article Title: Leishmania infection in blood donors: A new challenge in leishmaniasis transmission?
Article Snippet: .. Primers HM1 ( 5'-CCG CCC CTA TTT TAC ACC AAC CCC-3' ), HM2 ( 5'-GGG GAG GGG CGT TCT GCG AA-3' ), and HM3 ( 5'-GGC CCA CTA TAT TAC ACC AAC CCC-3' ) were employed to amplify the 120-bp fragment of the conserved region of Leishmania kDNA minicircle [ ]. kDNA-PCR was performed with a final 25 μL volume containing 1x Colorless GoTaq Flexi buffer (Promega, Madison, USA), 200 μM dNTPs (dATP, dCTP, dGTP, and dTTP; Promega), 1.5 mM MgCl2 , a 1 μM concentration of each primer , roughly 150 ng of extracted DNA, and water to complete the reaction. .. In all reactions, 2 μL of Leishmania ( Viannia ) braziliensis genomic DNA (MHOM/BR/1975/M2903, LIPMed-Fiocruz, Rio de Janeiro) served as the positive control.

Clone Assay:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ). .. Positive cosmid genomic clones were analyzed and sequenced, and respective cDNA was obtained by RT-PCR using specific primers designed based on the genomic sequence.

Plasmid Preparation:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ). .. Positive cosmid genomic clones were analyzed and sequenced, and respective cDNA was obtained by RT-PCR using specific primers designed based on the genomic sequence.

Polymerase Chain Reaction:

Article Title: Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica
Article Snippet: .. The 120-bp fragment was cloned into the PCR vector pGEM-T (Promega, Madison, WI) and used to screen a cosmid genomic EP44 library ( ). .. Positive cosmid genomic clones were analyzed and sequenced, and respective cDNA was obtained by RT-PCR using specific primers designed based on the genomic sequence.

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  • 85
    Promega murine ccn2 ctgf cdna
    Effects of <t>rCCN2/CTGF</t> on ALPase activity from MPL cells. When MPL cells reached confluence, the culture medium was replaced with α-MEM containing 1% FBS, and the cells were then cultured with various concentrations of rCCN2/CTGF. After 48 hrs, the cell layers were collected, and ALPase activity was determined as described in
    Murine Ccn2 Ctgf Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine ccn2 ctgf cdna/product/Promega
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    murine ccn2 ctgf cdna - by Bioz Stars, 2020-08
    85/100 stars
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    92
    Promega 261 bp fragment
    Binding of Fnr and ArcA to S . Enteritidis lpxO promoter region. EMSA were conducted using a 480 bp fragment carrying the complete lpxO promoter and purified FnrD154A (A) or P-ArcA (B) proteins. EMSA were also performed incubating purified P-ArcA with fragments of the lpxO promoter region containing ABS-1, ABS-2, or ABS-3 (C) . In all cases, EMSA included heat-inactivated proteins and/or non-phosphorylated ArcA as negative controls. When indicated, a <t>261</t> bp DNA fragment was used as non-specific competitor.
    261 Bp Fragment, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/261 bp fragment/product/Promega
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    261 bp fragment - by Bioz Stars, 2020-08
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    91
    Promega bst eii
    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with <t>Bst</t> <t>EII</t> (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.
    Bst Eii, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst eii/product/Promega
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bst eii - by Bioz Stars, 2020-08
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    89
    Promega ecori hindiii fragment
    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with <t>Bst</t> <t>EII</t> (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.
    Ecori Hindiii Fragment, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori hindiii fragment/product/Promega
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ecori hindiii fragment - by Bioz Stars, 2020-08
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    Image Search Results


    Effects of rCCN2/CTGF on ALPase activity from MPL cells. When MPL cells reached confluence, the culture medium was replaced with α-MEM containing 1% FBS, and the cells were then cultured with various concentrations of rCCN2/CTGF. After 48 hrs, the cell layers were collected, and ALPase activity was determined as described in

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Effects of rCCN2/CTGF on ALPase activity from MPL cells. When MPL cells reached confluence, the culture medium was replaced with α-MEM containing 1% FBS, and the cells were then cultured with various concentrations of rCCN2/CTGF. After 48 hrs, the cell layers were collected, and ALPase activity was determined as described in "Materials and Methods." Values represent the averages ± SD of 3 separate experiments. Asterisks denote statistically significant differences from the vehicle-treated control at the significance level of * p

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Activity Assay, Cell Culture

    RT-PCR analysis of the expression of CCN2/CTGF mRNA in sparse (S) and confluent (C) MPL cultures. Total RNA was purified from MPL cultures under sparse and confluent conditions, and 1 μg in each sample was reverse transcribed and used for PCR. The expression of CCN2/CTGF mRNA was stronger under the sparse than the confluent condition as evaluated in the exponential phase of the amplification (25 cycles). Levels of GAPDH mRNA, used as an internal control, were almost the same among these samples.

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: RT-PCR analysis of the expression of CCN2/CTGF mRNA in sparse (S) and confluent (C) MPL cultures. Total RNA was purified from MPL cultures under sparse and confluent conditions, and 1 μg in each sample was reverse transcribed and used for PCR. The expression of CCN2/CTGF mRNA was stronger under the sparse than the confluent condition as evaluated in the exponential phase of the amplification (25 cycles). Levels of GAPDH mRNA, used as an internal control, were almost the same among these samples.

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Purification, Polymerase Chain Reaction, Amplification

    (A) Effect of rCCN2/CTGF on DNA synthesis in MPL cells. MPL cells were inoculated at a density of 5 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 2 days. Then, the culture medium was changed to α-MEM with 0.5% FBS, and the cells were incubated with various concentrations of rCCN2/CTGF for 24 hrs and labeled with [ 3 H] thymidine for the last 4 hrs. The incorporated radioactivity was determined by a liquid scintillation counter. Values represent the averages ± SD. Data were computed with the results of 2 independent series of experiments with multiple sample numbers. Asterisks denote statistically significant difference from the vehicle-treated control. (B) Effect of rCCN2/CTGF on the proliferation of MPL cells. MPL cells were inoculated at a density of 2 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 24 hrs. Then, the medium was changed to α-MEM with 1% FBS, and the cells were cultured with various concentrations of rCCN2/CTGF for 3 days. The cell number was computed by using Tetra-Color One as specified by the manufacturer. Points and bars represent the averages and SD, respectively. Asterisks indicate significant differences between vehicle-treated control and rCCN2/CTGF-treated samples at the significance level of ** p

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: (A) Effect of rCCN2/CTGF on DNA synthesis in MPL cells. MPL cells were inoculated at a density of 5 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 2 days. Then, the culture medium was changed to α-MEM with 0.5% FBS, and the cells were incubated with various concentrations of rCCN2/CTGF for 24 hrs and labeled with [ 3 H] thymidine for the last 4 hrs. The incorporated radioactivity was determined by a liquid scintillation counter. Values represent the averages ± SD. Data were computed with the results of 2 independent series of experiments with multiple sample numbers. Asterisks denote statistically significant difference from the vehicle-treated control. (B) Effect of rCCN2/CTGF on the proliferation of MPL cells. MPL cells were inoculated at a density of 2 × 10 3 /well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 24 hrs. Then, the medium was changed to α-MEM with 1% FBS, and the cells were cultured with various concentrations of rCCN2/CTGF for 3 days. The cell number was computed by using Tetra-Color One as specified by the manufacturer. Points and bars represent the averages and SD, respectively. Asterisks indicate significant differences between vehicle-treated control and rCCN2/CTGF-treated samples at the significance level of ** p

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: DNA Synthesis, Cell Culture, Incubation, Labeling, Radioactivity

    Effects of rCCN2/CTGF on mRNA expression of osteoblast and PDL-related markers in MPL cells. Confluent cultures of MPL cells were treated with rCCN2/CTGF (100 ng/ml, closed bars) or vehicle (dotted bars) for 24 hrs. Total RNA was purified, and 1 μg of each sample was reverse-transcribed and used for PCR. Alkaline phosphatase (ALPase), type I collagen, and periostin mRNAs were shown to be up-regulated clearly by the addition of rCCN2/CTGF under exponential conditions (25, 30, and 25 cycles, respectively) for amplification. The expression of GAPDH, used for an internal control, was almost the same among these samples.

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Effects of rCCN2/CTGF on mRNA expression of osteoblast and PDL-related markers in MPL cells. Confluent cultures of MPL cells were treated with rCCN2/CTGF (100 ng/ml, closed bars) or vehicle (dotted bars) for 24 hrs. Total RNA was purified, and 1 μg of each sample was reverse-transcribed and used for PCR. Alkaline phosphatase (ALPase), type I collagen, and periostin mRNAs were shown to be up-regulated clearly by the addition of rCCN2/CTGF under exponential conditions (25, 30, and 25 cycles, respectively) for amplification. The expression of GAPDH, used for an internal control, was almost the same among these samples.

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Expressing, Purification, Polymerase Chain Reaction, Amplification

    Effect of rCCN2/CTGF on the synthesis of collagen and total protein in MPL cells. Various concentrations of rCCN2/CTGF were added to confluent cultures of MPL cells, and the cells were cultured for 12 hrs. Then, the cells were labeled with [ 3 H] proline for another 12 hrs, after which their cell layers were collected for analysis. The radioactivities of [ 3 H] proline incorporated into total nascent proteins and collagenase-digestable portions were measured as described in

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Effect of rCCN2/CTGF on the synthesis of collagen and total protein in MPL cells. Various concentrations of rCCN2/CTGF were added to confluent cultures of MPL cells, and the cells were cultured for 12 hrs. Then, the cells were labeled with [ 3 H] proline for another 12 hrs, after which their cell layers were collected for analysis. The radioactivities of [ 3 H] proline incorporated into total nascent proteins and collagenase-digestable portions were measured as described in "Materials and Methods." Slashed and closed boxes indicate the collagen and total protein synthesis, respectively. Values represent the mean average ± SD (n = 2). Asterisks denote statistically significant differences (** p

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Cell Culture, Labeling

    Expression of CCN2/CTGF mRNA in sparse and confluent MPL cultures (in situ hybridization) with or without TGF-β stimulation. MPL cultures in the sparsity phase (A and B) and confluence phase (C and D) were treated with TGF-β (B and D) or PBS (control: A and C). The cells were then cultured for 12 hrs, fixed, and hybridized with an antisense riboprobe for ccn2/ctgf mRNA. CCN2/CTGF mRNA was distinctly expressed in the cells in the sparse (A), but not in those in the confluent (C) state. However, the addition of TGF-β enhanced the level of CCN2/CTGF mRNA strongly, not only in the sparse cultures (B), but also in the confluent ones (D).

    Journal: Cell communication and signaling : CCS

    Article Title: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

    doi: 10.1186/1478-811X-3-11

    Figure Lengend Snippet: Expression of CCN2/CTGF mRNA in sparse and confluent MPL cultures (in situ hybridization) with or without TGF-β stimulation. MPL cultures in the sparsity phase (A and B) and confluence phase (C and D) were treated with TGF-β (B and D) or PBS (control: A and C). The cells were then cultured for 12 hrs, fixed, and hybridized with an antisense riboprobe for ccn2/ctgf mRNA. CCN2/CTGF mRNA was distinctly expressed in the cells in the sparse (A), but not in those in the confluent (C) state. However, the addition of TGF-β enhanced the level of CCN2/CTGF mRNA strongly, not only in the sparse cultures (B), but also in the confluent ones (D).

    Article Snippet: In situ hybridization For generation of the antisense probe, a 120-bp fragment of murine ccn2/ctgf cDNA corresponding to a non-coding region was subcloned into the pGEM-T plasmid (Promega, Madison, WI, USA), and riboprobes were synthesized by using a DIG RNA labelling kit (Roche, Mannheim, Germany) or 35 S-UTP(30TBq/nmol, Amersham Biosciences Corp.) following the manufacturer's instructions.

    Techniques: Expressing, In Situ Hybridization, Cell Culture

    Binding of Fnr and ArcA to S . Enteritidis lpxO promoter region. EMSA were conducted using a 480 bp fragment carrying the complete lpxO promoter and purified FnrD154A (A) or P-ArcA (B) proteins. EMSA were also performed incubating purified P-ArcA with fragments of the lpxO promoter region containing ABS-1, ABS-2, or ABS-3 (C) . In all cases, EMSA included heat-inactivated proteins and/or non-phosphorylated ArcA as negative controls. When indicated, a 261 bp DNA fragment was used as non-specific competitor.

    Journal: Frontiers in Microbiology

    Article Title: Fnr and ArcA Regulate Lipid A Hydroxylation in Salmonella Enteritidis by Controlling lpxO Expression in Response to Oxygen Availability

    doi: 10.3389/fmicb.2018.01220

    Figure Lengend Snippet: Binding of Fnr and ArcA to S . Enteritidis lpxO promoter region. EMSA were conducted using a 480 bp fragment carrying the complete lpxO promoter and purified FnrD154A (A) or P-ArcA (B) proteins. EMSA were also performed incubating purified P-ArcA with fragments of the lpxO promoter region containing ABS-1, ABS-2, or ABS-3 (C) . In all cases, EMSA included heat-inactivated proteins and/or non-phosphorylated ArcA as negative controls. When indicated, a 261 bp DNA fragment was used as non-specific competitor.

    Article Snippet: This fragment (120 ng) was incubated with different amounts of FnrD154A for 30 min at 37°C in reaction buffer supplemented with 50 μg BSA and 120 ng of a 261 bp fragment of non-specific competitor DNA corresponding to the polylinker region of pGEM-T Easy (Promega).

    Techniques: Binding Assay, Purification

    PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with Bst EII (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Differentiation of "Mycobacterium canettii" from Other Mycobacterium tuberculosis Complex Organisms by PCR-Restriction Analysis of the hsp65 Gene

    doi: 10.1128/JCM.39.10.3705-3708.2001

    Figure Lengend Snippet: PRA patterns of the M. tuberculosis complex organisms upon digestion of the 441-bp PCR product with Bst EII (A), Hae III (B), Aci I (C), or Hha I (D) and the sequence of the hsp65 fragment from “ M. canettii ” (E). Lanes 1, M. tuberculosis ; lanes 2, M. bovis ; lanes 3, M. bovis BCG; lanes 4, M. africanum ; lanes 5, “ M. canettii ”; lanes C, negative control; lanes M, 100-bp ladder. The underlined portion in panel E shows the single mutation at position 235 (C-to-T transition) that is linked to the disappearance of a Hha I site and that corresponds to position 631 of the homologous hsp65 gene of M. tuberculosis H37Rv.

    Article Snippet: Although the results were similar for all 102 isolates studied after the digestions with Bst EII (three fragments of 235, 120, and 80 bp; Fig. A), Hae III (four fragments of 155, 130, 70, and 40 bp; Fig. B), and Aci I (three fragments of 185, 130, 90 bp; Fig. C), the digestion of the 441-bp PCR product with Hha I clearly differentiated “ M. canettii ” from all other isolates at the subspecies level (Fig. D); it resulted in three fragments of 260, 105, and 60 bp for “ M. canettii ,” whereas it resulted in four fragments of 185, 105, 75, and 60 bp for M. tuberculosis , M. bovis , and M. bovis BCG.

    Techniques: Polymerase Chain Reaction, Sequencing, Negative Control, Mutagenesis