lactobacillus salivarius  (ATCC)

Journal: Scientific Reports

Article Title: Unravelling TPX2-centered co-expression networks as key drivers of aggressive prostate cancer

doi: 10.1038/s41598-025-27704-4

Figure Lengend Snippet: Shared genes and pathways between TPX2 WGCNA modules. ( a ) Upset plot showing shared DEGs between WGCNA modules related to TPX2 as a hub gene, including M3 (prim/norm), M2 (mHSPC/prim) and M1 (mCRPC/mHSPC). A total of 22 DEGs were shared among all three modules. ( b ) Heatmap of the unsupervised hierarchical clustering analysis on the variance-stabilized transformed (VST) expression of shared DEGs among all TPX2 modules as in (a). Each row corresponds to a single gene, whereas each column corresponds to a single sample. ( c ) Upset plot showing shared REACTOME pathways between modules as in (a). ( d ) A total of 20 pathways were shared among all three modules as in (a). The circle size is the gene ratio for each shared pathway in each comparison.

Article Snippet: TMA FFPE blocks were cut into 3 μm thick sections and manual IHC staining was performed using a Rabbit Anti-Human TPX2 antibody (11741-1-AP, Proteintech) diluted at 1:200.

Techniques: Transformation Assay, Expressing, Comparison

Journal: Scientific Reports

Article Title: Unravelling TPX2-centered co-expression networks as key drivers of aggressive prostate cancer

doi: 10.1038/s41598-025-27704-4

Figure Lengend Snippet: Validation of TPX2 protein expression. ( a ) Representative microscopic images of IHC staining of normal (norm), primary PCa (prim) and lymph node metastatic (met) PCa samples. Brown nuclear staining indicates TPX2 expression. Sections were counterstained with hematoxilin (blue color). ( b ) Protein expression levels indicated by H-scores, calculated from tissue microarrays containing 154 norm, 194 prim and 54 met tissue core biopsies. ( c ) Kaplan Meyer survival curves for high (red) and low (blue) expression levels of TPX2 . The expression cutoff (6.38) was determined by recursive partitioning. Hazard ratio (HR) including 95% confidence interval (CI) were determined by Cox proportional hazards analysis.

Article Snippet: TMA FFPE blocks were cut into 3 μm thick sections and manual IHC staining was performed using a Rabbit Anti-Human TPX2 antibody (11741-1-AP, Proteintech) diluted at 1:200.

Techniques: Biomarker Discovery, Expressing, Immunohistochemistry, Staining

Journal: Scientific Reports

Article Title: Unravelling TPX2-centered co-expression networks as key drivers of aggressive prostate cancer

doi: 10.1038/s41598-025-27704-4

Figure Lengend Snippet: Univariate Cox proportional hazards analysis of TPX2 -associated genes. Forest plot shows univariate Cox regression analysis of six TPX2 -associated genes ( TPX2 , MYBL2 , EXO1 , RRM2 , CENPA , and NEIL3 ), using biochemical recurrence (BCR) as the outcome. Hazard ratios (HR) with 95% confidence intervals (CI) are displayed for high vs. low expression groups, as defined by recursive partitioning (RP) cutpoints. After FDR correction, all six genes showed significant higher risk of BCR.

Article Snippet: TMA FFPE blocks were cut into 3 μm thick sections and manual IHC staining was performed using a Rabbit Anti-Human TPX2 antibody (11741-1-AP, Proteintech) diluted at 1:200.

Techniques: Expressing