fastprep instrument  (Valiant)

 
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    Name:
    FastPrep 96 demo instrument
    Description:

    Catalog Number:
    116010500d
    Price:
    0.0
    Category:
    Life Sciences Sample Preparation FastPrep Bead Beating Systems Bead Beating Instruments
    Size:
    1 Each
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    Structured Review

    Valiant fastprep instrument
    FastPrep 96 demo instrument

    https://www.bioz.com/result/fastprep instrument/product/Valiant
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fastprep instrument - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents"

    Article Title: Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

    Journal: MethodsX

    doi: 10.1016/j.mex.2015.03.007

    Concentrations of nucleic acids extracted (DNA and RNA) in function of the number of 40-s grinding cycles at 6 m s −1 performed by the FastPrep™ instrument. Errors bars correspond to 95% confidence intervals (alpha 0.05, 3 replicates). The conditions chosen as standard are framed.
    Figure Legend Snippet: Concentrations of nucleic acids extracted (DNA and RNA) in function of the number of 40-s grinding cycles at 6 m s −1 performed by the FastPrep™ instrument. Errors bars correspond to 95% confidence intervals (alpha 0.05, 3 replicates). The conditions chosen as standard are framed.

    Techniques Used:

    Related Articles

    RNA Extraction:

    Article Title: MicroRNA Expression Profiling Reveals MiRNA Families Regulating Specific Biological Pathways in Mouse Frontal Cortex and Hippocampus
    Article Snippet: Tissues were snap frozen in liquid nitrogen and stored at −80°C until RNA isolation. .. Total RNA extraction Tissue was homogenized using lysing matrixD tubes and the Fastprep instrument (Q.Biogene, Carlsbad, CA) according to the manufacturer's recommendation. .. The extraction of total RNA was performed using the TriReagent (Molecular Research Center, Cincinnati, OH) also according to the manufacturer's instructions.

    Isolation:

    Article Title: Rgg Influences the Expression of Multiple Regulatory Loci To Coregulate Virulence Factor Expression in Streptococcus pyogenes
    Article Snippet: Cultures were centrifuged, and the bacteria were suspended in 200 μl of diethyl pyrocarbonate (Sigma Chemical Co., St. Louis, Mo.)-treated water and frozen in liquid nitrogen. .. RNA was isolated with a FastPrep Instrument (Qbiogene, Carlsbad, Calif.) and a FastPrep Blue kit (Qbiogene) as previously described ( ). ..

    Lysis:

    Article Title: Biodiversity and Emerging Biogeography of the Neutrophilic Iron-Oxidizing Zetaproteobacteria ▿ ▿ †
    Article Snippet: Genomic DNA (gDNA) was extracted from samples using a Fast DNA Spin kit for soil (Qbiogene, Carlsbad, CA) according to the manufacturer's protocol, with the modification that gDNA was eluted into 10 mM Tris with 0.1 mM EDTA at pH 8 (TE). .. To optimize the cellular lysis step, a FastPrep instrument (Qbiogene) was used at an indexed speed of 5.5 for 30 s. The purity and concentration of gDNA were determined with a NanoDrop ND-1000 spectrophotometer. ..

    Concentration Assay:

    Article Title: Biodiversity and Emerging Biogeography of the Neutrophilic Iron-Oxidizing Zetaproteobacteria ▿ ▿ †
    Article Snippet: Genomic DNA (gDNA) was extracted from samples using a Fast DNA Spin kit for soil (Qbiogene, Carlsbad, CA) according to the manufacturer's protocol, with the modification that gDNA was eluted into 10 mM Tris with 0.1 mM EDTA at pH 8 (TE). .. To optimize the cellular lysis step, a FastPrep instrument (Qbiogene) was used at an indexed speed of 5.5 for 30 s. The purity and concentration of gDNA were determined with a NanoDrop ND-1000 spectrophotometer. ..

    Spectrophotometry:

    Article Title: Biodiversity and Emerging Biogeography of the Neutrophilic Iron-Oxidizing Zetaproteobacteria ▿ ▿ †
    Article Snippet: Genomic DNA (gDNA) was extracted from samples using a Fast DNA Spin kit for soil (Qbiogene, Carlsbad, CA) according to the manufacturer's protocol, with the modification that gDNA was eluted into 10 mM Tris with 0.1 mM EDTA at pH 8 (TE). .. To optimize the cellular lysis step, a FastPrep instrument (Qbiogene) was used at an indexed speed of 5.5 for 30 s. The purity and concentration of gDNA were determined with a NanoDrop ND-1000 spectrophotometer. ..

    Size-exclusion Chromatography:

    Article Title: Assessment of the human fecal microbiota: II. Reproducibility and associations of 16S rRNA pyrosequences
    Article Snippet: The mixture was incubated for 1 hour at 37°C, following which 10 μL proteinase K (20 mg/ml), 100 μL 10% SDS, and 20 μL RNase A (20 mg/ml) were added. .. To further lyse microbial cells, mechanical disruption (bead beating) was performed on the mixture using a FastPrep instrument (MP Biomedicals, Solon, OH) set at 6.0 m/s for 30 sec. .. The lysate was processed using the ZR Fecal DNA extraction kit (ZYMO Research, Irvine, CA) according to the manufacturer's recommendation omitting the lysis steps (steps 1–3).

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    Valiant fastprep instrument
    Concentrations of nucleic acids extracted (DNA and RNA) in function of the number of 40-s grinding cycles at 6 m s −1 performed by the <t>FastPrep™</t> instrument. Errors bars correspond to 95% confidence intervals (alpha 0.05, 3 replicates). The conditions chosen as standard are framed.
    Fastprep Instrument, supplied by Valiant, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastprep instrument/product/Valiant
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fastprep instrument - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

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    Concentrations of nucleic acids extracted (DNA and RNA) in function of the number of 40-s grinding cycles at 6 m s −1 performed by the FastPrep™ instrument. Errors bars correspond to 95% confidence intervals (alpha 0.05, 3 replicates). The conditions chosen as standard are framed.

    Journal: MethodsX

    Article Title: Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

    doi: 10.1016/j.mex.2015.03.007

    Figure Lengend Snippet: Concentrations of nucleic acids extracted (DNA and RNA) in function of the number of 40-s grinding cycles at 6 m s −1 performed by the FastPrep™ instrument. Errors bars correspond to 95% confidence intervals (alpha 0.05, 3 replicates). The conditions chosen as standard are framed.

    Article Snippet: Grinding: homogenize in the FastPrep™ instrument (MP Biomedical) for 40 s at a speed setting of 6.0.

    Techniques: