adenine n1 oxide  (Valiant)


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  • 93
    Name:
    Adenine
    Description:

    Catalog Number:
    114060012
    Price:
    45.0
    Size:
    10 g
    Category:
    Nucleotides Peptides
    Buy from Supplier


    Structured Review

    Valiant adenine n1 oxide
    Adenine

    https://www.bioz.com/result/adenine n1 oxide/product/Valiant
    Average 93 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    adenine n1 oxide - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "Anti-inflammatory effects of adenosine N1-oxide"

    Article Title: Anti-inflammatory effects of adenosine N1-oxide

    Journal: Journal of Inflammation (London, England)

    doi: 10.1186/s12950-014-0045-0

    Inhibitory effects of ANO, adenosine, adenine and adenine N1-oxide on TNF-α and IL-6 secretion by LPS/muIFN-γ-stimulated peritoneal macrophages. Peritoneal macrophages (5 × 10 4 /well) were stimulated with LPS (1 μg/mL) and muIFN-γ (10 IU/mL) in the presence or absence of various concentrations of ANO, adenosine, adenine and adenine N1-oxide at 37°C for 24 h. Levels of TNF-α (A) and IL-6 (B) in the culture supernatants and growth of macrophages (C) were determined as described in Figure 1 . Values represent the means ± S.D. of quadruplicate cultures. Results are representative of two separate experiments with similar results. * p
    Figure Legend Snippet: Inhibitory effects of ANO, adenosine, adenine and adenine N1-oxide on TNF-α and IL-6 secretion by LPS/muIFN-γ-stimulated peritoneal macrophages. Peritoneal macrophages (5 × 10 4 /well) were stimulated with LPS (1 μg/mL) and muIFN-γ (10 IU/mL) in the presence or absence of various concentrations of ANO, adenosine, adenine and adenine N1-oxide at 37°C for 24 h. Levels of TNF-α (A) and IL-6 (B) in the culture supernatants and growth of macrophages (C) were determined as described in Figure 1 . Values represent the means ± S.D. of quadruplicate cultures. Results are representative of two separate experiments with similar results. * p

    Techniques Used:

    2) Product Images from "Outer Dynein Arm Light Chain 1 Is Essential for Controlling the Ciliary Response to Cyclic AMP in Paramecium tetraurelia"

    Article Title: Outer Dynein Arm Light Chain 1 Is Essential for Controlling the Ciliary Response to Cyclic AMP in Paramecium tetraurelia

    Journal: Eukaryotic Cell

    doi: 10.1128/EC.05279-11

    cAMP-dependent phosphorylation of the axonemal proteins from  ODAL1 -RNAi cells. Axonemes were labeled  in vitro  with adenosine 5′-[γ- 32 P]triphosphate. The phosphorylated proteins were run on 3-to-15% linear gradient acrylamide gels. CBB,
    Figure Legend Snippet: cAMP-dependent phosphorylation of the axonemal proteins from ODAL1 -RNAi cells. Axonemes were labeled in vitro with adenosine 5′-[γ- 32 P]triphosphate. The phosphorylated proteins were run on 3-to-15% linear gradient acrylamide gels. CBB,

    Techniques Used: Labeling, In Vitro

    Related Articles

    Amplification:

    Article Title: Association Between a Functional Variant Downstream of TNFAIP3 and Systemic Lupus Erythematosus
    Article Snippet: .. The amplified product was gel-purified and end-labeled with (γ-32 P) adenosine triphosphate (MP Biomedicals Int.) using T4 polynucleotide kinase (Invitrogen). .. A portion of each probe preparation was sequenced to verify no mutations had been introduced during amplification.

    Synthesized:

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: .. Electrophoretic mobility shift assays 40 base pair (non-risk) or 39 bp (risk) DNA probes were synthesized and end-labeled with (γ-32 P) adenosine triphosphate (MP Biomedicals Int.) using T4 polynucleotide kinase (Invitrogen, Grand Island, NY). .. Nuclear protein extracts were prepared from cells stimulated with LPS (1 ug/mL) or P/I (50 ng/ml, 500 ng/ml) for 2 hours and incubated for 25 min at 37°C with labeled probes in binding buffer (1 ug poly dI-dC, 20 mM HEPES, 10% Glycerol, 100 mM KCl, and 0.2 mM EDTA, pH 7.9).

    Labeling:

    Article Title: Incorporation of 5-chlorocytosine into mammalian DNA results in heritable gene silencing and altered cytosine methylation patterns
    Article Snippet: .. For the polymerase incorporation assays, the 22mer primer, derived from the hamster hprt promoter, was 5′-32 P-end labeled by T4 polynucleotide kinase (New England Biolabs, Ipswich, MA) with [γ-32 P]adenosine triphosphate (MP Biomedicals, Costa Mesa, CA) under conditions recommended by the enzyme supplier and subsequently purified using G50 Sephadex columns (Roche, Indianapolis, IN). .. The labeled 22mer was mixed with a 2-fold excess of the complementary 30mer strand in 100 mM Tris–HCl pH 7.0, incubated at 95°C for 5 min and allowed to cool to room temperature slowly to create a duplex with a 5′ overhang that provides a template for Klenow exo minus fragment ( ).

    Purification:

    Article Title: Incorporation of 5-chlorocytosine into mammalian DNA results in heritable gene silencing and altered cytosine methylation patterns
    Article Snippet: .. For the polymerase incorporation assays, the 22mer primer, derived from the hamster hprt promoter, was 5′-32 P-end labeled by T4 polynucleotide kinase (New England Biolabs, Ipswich, MA) with [γ-32 P]adenosine triphosphate (MP Biomedicals, Costa Mesa, CA) under conditions recommended by the enzyme supplier and subsequently purified using G50 Sephadex columns (Roche, Indianapolis, IN). .. The labeled 22mer was mixed with a 2-fold excess of the complementary 30mer strand in 100 mM Tris–HCl pH 7.0, incubated at 95°C for 5 min and allowed to cool to room temperature slowly to create a duplex with a 5′ overhang that provides a template for Klenow exo minus fragment ( ).

    Concentration Assay:

    Article Title: Outer Dynein Arm Light Chain 1 Is Essential for Controlling the Ciliary Response to Cyclic AMP in Paramecium tetraurelia
    Article Snippet: .. The ATP concentration of [γ-32 P]ATP was 10 μM, and the radioactivity was adjusted to 10 μCi with adenosine 5′-[γ-32 P]triphosphate (specific activity, 6,000 Ci/mmol; MP Biomedicals Inc., Solon, OH). ..

    Incubation:

    Article Title: Systemic immunotherapy delays photoreceptor cell loss and prevents vascular pathology in Royal College of Surgeons rats
    Article Snippet: .. The retinas were then transferred to a new vial containing 2 ml of 0.02% beta-nicotinamide adenine dinucleotide phosphate (NADPH)-reduced tetrasodium salt (MP Biomedicals, Solon, OH), 0.04% nitroblue tetrazolium chloride (Fisher Scientific, Pittsburgh, PA), and 3% Triton X-100 (Sigma) in PBS and incubated on a shaker for 2 h at room temperature. .. Next, the retinas were gently washed with PBS for 30 min and mounted on glass slides, dehydrated with alcohol, and covered with DPX Mounting Medium (Leica Microsoft, Buffalo Grove, IL).

    Radioactivity:

    Article Title: Outer Dynein Arm Light Chain 1 Is Essential for Controlling the Ciliary Response to Cyclic AMP in Paramecium tetraurelia
    Article Snippet: .. The ATP concentration of [γ-32 P]ATP was 10 μM, and the radioactivity was adjusted to 10 μCi with adenosine 5′-[γ-32 P]triphosphate (specific activity, 6,000 Ci/mmol; MP Biomedicals Inc., Solon, OH). ..

    Activity Assay:

    Article Title: Co-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins
    Article Snippet: .. [γ32 P]-adenosine triphosphate (ATP) (specific activity 6000 Ci/mmol) was from MP Biomedicals. ..

    Article Title: Outer Dynein Arm Light Chain 1 Is Essential for Controlling the Ciliary Response to Cyclic AMP in Paramecium tetraurelia
    Article Snippet: .. The ATP concentration of [γ-32 P]ATP was 10 μM, and the radioactivity was adjusted to 10 μCi with adenosine 5′-[γ-32 P]triphosphate (specific activity, 6,000 Ci/mmol; MP Biomedicals Inc., Solon, OH). ..

    Electrophoretic Mobility Shift Assay:

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: .. Electrophoretic mobility shift assays 40 base pair (non-risk) or 39 bp (risk) DNA probes were synthesized and end-labeled with (γ-32 P) adenosine triphosphate (MP Biomedicals Int.) using T4 polynucleotide kinase (Invitrogen, Grand Island, NY). .. Nuclear protein extracts were prepared from cells stimulated with LPS (1 ug/mL) or P/I (50 ng/ml, 500 ng/ml) for 2 hours and incubated for 25 min at 37°C with labeled probes in binding buffer (1 ug poly dI-dC, 20 mM HEPES, 10% Glycerol, 100 mM KCl, and 0.2 mM EDTA, pH 7.9).

    other:

    Article Title: Anti-inflammatory effects of adenosine N1-oxide
    Article Snippet: Adenine N1-Oxide was purchased from MP Biomedicals (Santa Ana, CA).

    Derivative Assay:

    Article Title: Incorporation of 5-chlorocytosine into mammalian DNA results in heritable gene silencing and altered cytosine methylation patterns
    Article Snippet: .. For the polymerase incorporation assays, the 22mer primer, derived from the hamster hprt promoter, was 5′-32 P-end labeled by T4 polynucleotide kinase (New England Biolabs, Ipswich, MA) with [γ-32 P]adenosine triphosphate (MP Biomedicals, Costa Mesa, CA) under conditions recommended by the enzyme supplier and subsequently purified using G50 Sephadex columns (Roche, Indianapolis, IN). .. The labeled 22mer was mixed with a 2-fold excess of the complementary 30mer strand in 100 mM Tris–HCl pH 7.0, incubated at 95°C for 5 min and allowed to cool to room temperature slowly to create a duplex with a 5′ overhang that provides a template for Klenow exo minus fragment ( ).