yp raffinose  (Valiant)

 
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  • 93
    Name:
    Raffinose
    Description:
    Raffinose is a trisaccharide composed of galactose fructose and glucose that is used as carbon source in yeast culture media
    Catalog Number:
    114010032
    Price:
    1577.0
    Category:
    Life Sciences Biochemicals Carbohydrates Polysaccharides
    Applications:
    Cell culture ,Proteomics ,Purification and Detection ,Protein Analysis ,Yeast Culture ,Plasmid Production ,Protein Expression ,Gene Expression ,Cloning ,Gene Function ,Gene Regulation ,Automated Bacterial Growth Media ,S. cerevisiae Culture
    Size:
    1 kg
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    Structured Review

    Valiant yp raffinose
    Raffinose
    Raffinose is a trisaccharide composed of galactose fructose and glucose that is used as carbon source in yeast culture media
    https://www.bioz.com/result/yp raffinose/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    yp raffinose - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN
    Article Snippet: For TAP-tag experiment (Open Biosystems), yGPN1-interacting partners were isolated from yeast grown in 2 L of yeast extract/peptone/dextrose liquid medium until an OD at 600 nm of 0.8 was reached. .. For two-hybrid assays, cells were cultured in raffinose synthetic complete medium minus uracile and histidine (MP Biomedicals) supplemented with 0.2% of galactose synthetic complete medium minus uracile and histidine at 30°C. .. Yeast proteins for western blot were extracted from cells grown in raffinose synthetic complete medium minus histidine supplemented with 10% of galactose synthetic complete medium minus histidine at 30°C.

    Polymerase Chain Reaction:

    Article Title: An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
    Article Snippet: Pipette tips Reusable cotton-plug top serological (glass) pipettes (can also use single-use plastic pipettes) 10 ml pipettes (Fisher Scientific, catalog number: 13-675M) 25 ml pipettes (Fisher Scientific, catalog number: 13-675N) Pipette sterilizing boxes (Fisher Scientific, catalog number: 03-465) Nalgene PPCO centrifuge bottles with sealing closure (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 3141-0500) 50 ml conical tubes 30 × 115 mm (Corning, Falcon® , catalog number: 352070) 2.0 ml microcentrifuge conical screw cap tubes (FastPrep tubes, Fisher Scientific, catalog number: 02-681-344) Microcentrifuge tube screw caps with O-Rings (for FastPrep tubes, Fisher Scientific, catalog number: 02-681-366) Acid washed 425-600 μm glass beads (Sigma-Aldrich, catalog number: G8772) 18 gauge needle (PrecisionGlide, BD, catalog number: 305195) Disposable culture tubes, glass 13 × 100 mm (Fisher Scientific, catalog number: 14-961-27) 1.5 ml microcentrifuge tubes graduated (Fisher Scientific, catalog number: 05-408-129) Whatman filter paper (GE Healthcare, catalog number: 1003-917) Autoradiography film (Genesee Scientific, catalog number: 30-101) 1.5-1.7 ml polypropylene graduated tube with locking lid (Fisher Scientific, catalog number: 02-681-285) Hard-Shell High-Profile 96-well semi-skirted PCR plates (Bio-Rad Laboratories, catalog number: HSS9641) Tubular roll stock (to seal membrane in plastic before developing; Ampac, catalog number: TRS-95125-3) Immobilon-P membrane (PVDF; Merck, catalog number: IPVH00010) Microseal ‘B’ seal seals (Bio-Rad Laboratories, catalog number: MSB1001) Stericup sterile vacuum filter units, 500 ml (Merck, catalog number: SCGPU05RE) Yeast strains, wild type (WT) and overexpression (OE) for each TF of interest (see and for strain lists) Plasmids for TF overexpression and vector control for WT strain (see and for plasmid lists) Locus specific primers (Invitrogen) In-Fusion HD cloning kit (Takara Bio, Clontech, catalog number: 639649) Ice bucket 37% formaldehyde (Fisher Scientific, catalog number: F79) Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, catalog number: 500-0006) nProtein A Sepharose 4 Fast Flow beads (GE Healthcare, catalog number: 17-5280-01) Note: Ab-conjugated beads can be used instead, such as IgG Sepharose 6 Fast Flow (GE Healthcare, catalog number: 17-0969-01) and Sepharose 6 Fast Flow beads (GE Healthcare, catalog number: 17-0159-99) . .. QIAQuick PCR purification kit (QIAGEN, catalog number: 28106) iQ SYBR Green Supermix, 500 × 50 μI rxns (Bio-Rad Laboratories, catalog number: 1708882) Instant non-fat dry milk (Carnation) Antibody ( i.e. , α-TBP, monoclonal, Abcam, catalog number: ab61411) Secondary ECL-conjugated antibody (we use Amersham ECL mouse or rabbit IgG HRP-linked whole Ab, GE Healthcare, catalog numbers: NXA931 or NA934V) Amersham ECL Prime Western blotting detecting reagent (GE Healthcare, catalog number: RPN2232) Yeast extract (BD, Bacto™, catalog number: 212750) Bacto peptone (BD, Bacto™, catalog number: 211677) Sugar source, i.e. ,: D-(+)-Glucose (Sigma-Aldrich, catalog number: G7021) D-(+)-Galactose (Sigma-Aldrich, catalog number: G5388) D-(+)-Raffinose (MP Biomedicals, catalog number: 02102797) Difco yeast nitrogen base without amino acids and ammonium sulfate (BD, Difco™, catalog number: 233520) Note: Yeast nitrogen base without amino acids (with or without sugar source) can be used as an alternative and doesn’t require the addition of ammonium sulfate (i.e., Sigma-Aldrich, catalog number: Y0626) . .. Amino acids: Adenine hemisulfate dihydrate (MP Biomedicals, catalog number: 02100195) L-Histidine hydrochloride monohydrate (Acros Organics, catalog number: 411731000) L-Lysine (Fisher Scientific, catalog number: BP386) L-Tyrosine (Acros Organics, catalog number: 140641000) L-Tryptophan (Fisher Scientific, catalog number: BP395) Uracil (Affymetrix, catalog number: 23020) L-Leucine (Acros Organics, catalog number: 125121000) L-Methionine (Fisher Scientific, catalog number: BP388) L-Arginine hydrochloride (Fisher Scientific, catalog number: BP372) L-Serine (Fisher Scientific, catalog number: BP393) Valine (Fisher Scientific, catalog number: BP397) L-Threonine (MP Biomedicals, catalog number: 02103053) L-Isoleucine (Fisher Scientific, catalog number: BP384) L-Phenylalanine (Fisher Scientific, catalog number: BP391) L-Cysteine hydrochloride monohydrate (Fisher Scientific, catalog number: BP376) L-Aspartic Acid (Acros Organics, catalog number: 105041000) L-Proline (Fisher Scientific, catalog number: BP392) Note: Commercially available yeast synthetic drop-out medium supplements (Sigma-Aldrich) can be used instead of a home-made dropout mix .

    Purification:

    Article Title: An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
    Article Snippet: Pipette tips Reusable cotton-plug top serological (glass) pipettes (can also use single-use plastic pipettes) 10 ml pipettes (Fisher Scientific, catalog number: 13-675M) 25 ml pipettes (Fisher Scientific, catalog number: 13-675N) Pipette sterilizing boxes (Fisher Scientific, catalog number: 03-465) Nalgene PPCO centrifuge bottles with sealing closure (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 3141-0500) 50 ml conical tubes 30 × 115 mm (Corning, Falcon® , catalog number: 352070) 2.0 ml microcentrifuge conical screw cap tubes (FastPrep tubes, Fisher Scientific, catalog number: 02-681-344) Microcentrifuge tube screw caps with O-Rings (for FastPrep tubes, Fisher Scientific, catalog number: 02-681-366) Acid washed 425-600 μm glass beads (Sigma-Aldrich, catalog number: G8772) 18 gauge needle (PrecisionGlide, BD, catalog number: 305195) Disposable culture tubes, glass 13 × 100 mm (Fisher Scientific, catalog number: 14-961-27) 1.5 ml microcentrifuge tubes graduated (Fisher Scientific, catalog number: 05-408-129) Whatman filter paper (GE Healthcare, catalog number: 1003-917) Autoradiography film (Genesee Scientific, catalog number: 30-101) 1.5-1.7 ml polypropylene graduated tube with locking lid (Fisher Scientific, catalog number: 02-681-285) Hard-Shell High-Profile 96-well semi-skirted PCR plates (Bio-Rad Laboratories, catalog number: HSS9641) Tubular roll stock (to seal membrane in plastic before developing; Ampac, catalog number: TRS-95125-3) Immobilon-P membrane (PVDF; Merck, catalog number: IPVH00010) Microseal ‘B’ seal seals (Bio-Rad Laboratories, catalog number: MSB1001) Stericup sterile vacuum filter units, 500 ml (Merck, catalog number: SCGPU05RE) Yeast strains, wild type (WT) and overexpression (OE) for each TF of interest (see and for strain lists) Plasmids for TF overexpression and vector control for WT strain (see and for plasmid lists) Locus specific primers (Invitrogen) In-Fusion HD cloning kit (Takara Bio, Clontech, catalog number: 639649) Ice bucket 37% formaldehyde (Fisher Scientific, catalog number: F79) Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, catalog number: 500-0006) nProtein A Sepharose 4 Fast Flow beads (GE Healthcare, catalog number: 17-5280-01) Note: Ab-conjugated beads can be used instead, such as IgG Sepharose 6 Fast Flow (GE Healthcare, catalog number: 17-0969-01) and Sepharose 6 Fast Flow beads (GE Healthcare, catalog number: 17-0159-99) . .. QIAQuick PCR purification kit (QIAGEN, catalog number: 28106) iQ SYBR Green Supermix, 500 × 50 μI rxns (Bio-Rad Laboratories, catalog number: 1708882) Instant non-fat dry milk (Carnation) Antibody ( i.e. , α-TBP, monoclonal, Abcam, catalog number: ab61411) Secondary ECL-conjugated antibody (we use Amersham ECL mouse or rabbit IgG HRP-linked whole Ab, GE Healthcare, catalog numbers: NXA931 or NA934V) Amersham ECL Prime Western blotting detecting reagent (GE Healthcare, catalog number: RPN2232) Yeast extract (BD, Bacto™, catalog number: 212750) Bacto peptone (BD, Bacto™, catalog number: 211677) Sugar source, i.e. ,: D-(+)-Glucose (Sigma-Aldrich, catalog number: G7021) D-(+)-Galactose (Sigma-Aldrich, catalog number: G5388) D-(+)-Raffinose (MP Biomedicals, catalog number: 02102797) Difco yeast nitrogen base without amino acids and ammonium sulfate (BD, Difco™, catalog number: 233520) Note: Yeast nitrogen base without amino acids (with or without sugar source) can be used as an alternative and doesn’t require the addition of ammonium sulfate (i.e., Sigma-Aldrich, catalog number: Y0626) . .. Amino acids: Adenine hemisulfate dihydrate (MP Biomedicals, catalog number: 02100195) L-Histidine hydrochloride monohydrate (Acros Organics, catalog number: 411731000) L-Lysine (Fisher Scientific, catalog number: BP386) L-Tyrosine (Acros Organics, catalog number: 140641000) L-Tryptophan (Fisher Scientific, catalog number: BP395) Uracil (Affymetrix, catalog number: 23020) L-Leucine (Acros Organics, catalog number: 125121000) L-Methionine (Fisher Scientific, catalog number: BP388) L-Arginine hydrochloride (Fisher Scientific, catalog number: BP372) L-Serine (Fisher Scientific, catalog number: BP393) Valine (Fisher Scientific, catalog number: BP397) L-Threonine (MP Biomedicals, catalog number: 02103053) L-Isoleucine (Fisher Scientific, catalog number: BP384) L-Phenylalanine (Fisher Scientific, catalog number: BP391) L-Cysteine hydrochloride monohydrate (Fisher Scientific, catalog number: BP376) L-Aspartic Acid (Acros Organics, catalog number: 105041000) L-Proline (Fisher Scientific, catalog number: BP392) Note: Commercially available yeast synthetic drop-out medium supplements (Sigma-Aldrich) can be used instead of a home-made dropout mix .

    SYBR Green Assay:

    Article Title: An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
    Article Snippet: Pipette tips Reusable cotton-plug top serological (glass) pipettes (can also use single-use plastic pipettes) 10 ml pipettes (Fisher Scientific, catalog number: 13-675M) 25 ml pipettes (Fisher Scientific, catalog number: 13-675N) Pipette sterilizing boxes (Fisher Scientific, catalog number: 03-465) Nalgene PPCO centrifuge bottles with sealing closure (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 3141-0500) 50 ml conical tubes 30 × 115 mm (Corning, Falcon® , catalog number: 352070) 2.0 ml microcentrifuge conical screw cap tubes (FastPrep tubes, Fisher Scientific, catalog number: 02-681-344) Microcentrifuge tube screw caps with O-Rings (for FastPrep tubes, Fisher Scientific, catalog number: 02-681-366) Acid washed 425-600 μm glass beads (Sigma-Aldrich, catalog number: G8772) 18 gauge needle (PrecisionGlide, BD, catalog number: 305195) Disposable culture tubes, glass 13 × 100 mm (Fisher Scientific, catalog number: 14-961-27) 1.5 ml microcentrifuge tubes graduated (Fisher Scientific, catalog number: 05-408-129) Whatman filter paper (GE Healthcare, catalog number: 1003-917) Autoradiography film (Genesee Scientific, catalog number: 30-101) 1.5-1.7 ml polypropylene graduated tube with locking lid (Fisher Scientific, catalog number: 02-681-285) Hard-Shell High-Profile 96-well semi-skirted PCR plates (Bio-Rad Laboratories, catalog number: HSS9641) Tubular roll stock (to seal membrane in plastic before developing; Ampac, catalog number: TRS-95125-3) Immobilon-P membrane (PVDF; Merck, catalog number: IPVH00010) Microseal ‘B’ seal seals (Bio-Rad Laboratories, catalog number: MSB1001) Stericup sterile vacuum filter units, 500 ml (Merck, catalog number: SCGPU05RE) Yeast strains, wild type (WT) and overexpression (OE) for each TF of interest (see and for strain lists) Plasmids for TF overexpression and vector control for WT strain (see and for plasmid lists) Locus specific primers (Invitrogen) In-Fusion HD cloning kit (Takara Bio, Clontech, catalog number: 639649) Ice bucket 37% formaldehyde (Fisher Scientific, catalog number: F79) Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, catalog number: 500-0006) nProtein A Sepharose 4 Fast Flow beads (GE Healthcare, catalog number: 17-5280-01) Note: Ab-conjugated beads can be used instead, such as IgG Sepharose 6 Fast Flow (GE Healthcare, catalog number: 17-0969-01) and Sepharose 6 Fast Flow beads (GE Healthcare, catalog number: 17-0159-99) . .. QIAQuick PCR purification kit (QIAGEN, catalog number: 28106) iQ SYBR Green Supermix, 500 × 50 μI rxns (Bio-Rad Laboratories, catalog number: 1708882) Instant non-fat dry milk (Carnation) Antibody ( i.e. , α-TBP, monoclonal, Abcam, catalog number: ab61411) Secondary ECL-conjugated antibody (we use Amersham ECL mouse or rabbit IgG HRP-linked whole Ab, GE Healthcare, catalog numbers: NXA931 or NA934V) Amersham ECL Prime Western blotting detecting reagent (GE Healthcare, catalog number: RPN2232) Yeast extract (BD, Bacto™, catalog number: 212750) Bacto peptone (BD, Bacto™, catalog number: 211677) Sugar source, i.e. ,: D-(+)-Glucose (Sigma-Aldrich, catalog number: G7021) D-(+)-Galactose (Sigma-Aldrich, catalog number: G5388) D-(+)-Raffinose (MP Biomedicals, catalog number: 02102797) Difco yeast nitrogen base without amino acids and ammonium sulfate (BD, Difco™, catalog number: 233520) Note: Yeast nitrogen base without amino acids (with or without sugar source) can be used as an alternative and doesn’t require the addition of ammonium sulfate (i.e., Sigma-Aldrich, catalog number: Y0626) . .. Amino acids: Adenine hemisulfate dihydrate (MP Biomedicals, catalog number: 02100195) L-Histidine hydrochloride monohydrate (Acros Organics, catalog number: 411731000) L-Lysine (Fisher Scientific, catalog number: BP386) L-Tyrosine (Acros Organics, catalog number: 140641000) L-Tryptophan (Fisher Scientific, catalog number: BP395) Uracil (Affymetrix, catalog number: 23020) L-Leucine (Acros Organics, catalog number: 125121000) L-Methionine (Fisher Scientific, catalog number: BP388) L-Arginine hydrochloride (Fisher Scientific, catalog number: BP372) L-Serine (Fisher Scientific, catalog number: BP393) Valine (Fisher Scientific, catalog number: BP397) L-Threonine (MP Biomedicals, catalog number: 02103053) L-Isoleucine (Fisher Scientific, catalog number: BP384) L-Phenylalanine (Fisher Scientific, catalog number: BP391) L-Cysteine hydrochloride monohydrate (Fisher Scientific, catalog number: BP376) L-Aspartic Acid (Acros Organics, catalog number: 105041000) L-Proline (Fisher Scientific, catalog number: BP392) Note: Commercially available yeast synthetic drop-out medium supplements (Sigma-Aldrich) can be used instead of a home-made dropout mix .

    Western Blot:

    Article Title: An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
    Article Snippet: Pipette tips Reusable cotton-plug top serological (glass) pipettes (can also use single-use plastic pipettes) 10 ml pipettes (Fisher Scientific, catalog number: 13-675M) 25 ml pipettes (Fisher Scientific, catalog number: 13-675N) Pipette sterilizing boxes (Fisher Scientific, catalog number: 03-465) Nalgene PPCO centrifuge bottles with sealing closure (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 3141-0500) 50 ml conical tubes 30 × 115 mm (Corning, Falcon® , catalog number: 352070) 2.0 ml microcentrifuge conical screw cap tubes (FastPrep tubes, Fisher Scientific, catalog number: 02-681-344) Microcentrifuge tube screw caps with O-Rings (for FastPrep tubes, Fisher Scientific, catalog number: 02-681-366) Acid washed 425-600 μm glass beads (Sigma-Aldrich, catalog number: G8772) 18 gauge needle (PrecisionGlide, BD, catalog number: 305195) Disposable culture tubes, glass 13 × 100 mm (Fisher Scientific, catalog number: 14-961-27) 1.5 ml microcentrifuge tubes graduated (Fisher Scientific, catalog number: 05-408-129) Whatman filter paper (GE Healthcare, catalog number: 1003-917) Autoradiography film (Genesee Scientific, catalog number: 30-101) 1.5-1.7 ml polypropylene graduated tube with locking lid (Fisher Scientific, catalog number: 02-681-285) Hard-Shell High-Profile 96-well semi-skirted PCR plates (Bio-Rad Laboratories, catalog number: HSS9641) Tubular roll stock (to seal membrane in plastic before developing; Ampac, catalog number: TRS-95125-3) Immobilon-P membrane (PVDF; Merck, catalog number: IPVH00010) Microseal ‘B’ seal seals (Bio-Rad Laboratories, catalog number: MSB1001) Stericup sterile vacuum filter units, 500 ml (Merck, catalog number: SCGPU05RE) Yeast strains, wild type (WT) and overexpression (OE) for each TF of interest (see and for strain lists) Plasmids for TF overexpression and vector control for WT strain (see and for plasmid lists) Locus specific primers (Invitrogen) In-Fusion HD cloning kit (Takara Bio, Clontech, catalog number: 639649) Ice bucket 37% formaldehyde (Fisher Scientific, catalog number: F79) Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories, catalog number: 500-0006) nProtein A Sepharose 4 Fast Flow beads (GE Healthcare, catalog number: 17-5280-01) Note: Ab-conjugated beads can be used instead, such as IgG Sepharose 6 Fast Flow (GE Healthcare, catalog number: 17-0969-01) and Sepharose 6 Fast Flow beads (GE Healthcare, catalog number: 17-0159-99) . .. QIAQuick PCR purification kit (QIAGEN, catalog number: 28106) iQ SYBR Green Supermix, 500 × 50 μI rxns (Bio-Rad Laboratories, catalog number: 1708882) Instant non-fat dry milk (Carnation) Antibody ( i.e. , α-TBP, monoclonal, Abcam, catalog number: ab61411) Secondary ECL-conjugated antibody (we use Amersham ECL mouse or rabbit IgG HRP-linked whole Ab, GE Healthcare, catalog numbers: NXA931 or NA934V) Amersham ECL Prime Western blotting detecting reagent (GE Healthcare, catalog number: RPN2232) Yeast extract (BD, Bacto™, catalog number: 212750) Bacto peptone (BD, Bacto™, catalog number: 211677) Sugar source, i.e. ,: D-(+)-Glucose (Sigma-Aldrich, catalog number: G7021) D-(+)-Galactose (Sigma-Aldrich, catalog number: G5388) D-(+)-Raffinose (MP Biomedicals, catalog number: 02102797) Difco yeast nitrogen base without amino acids and ammonium sulfate (BD, Difco™, catalog number: 233520) Note: Yeast nitrogen base without amino acids (with or without sugar source) can be used as an alternative and doesn’t require the addition of ammonium sulfate (i.e., Sigma-Aldrich, catalog number: Y0626) . .. Amino acids: Adenine hemisulfate dihydrate (MP Biomedicals, catalog number: 02100195) L-Histidine hydrochloride monohydrate (Acros Organics, catalog number: 411731000) L-Lysine (Fisher Scientific, catalog number: BP386) L-Tyrosine (Acros Organics, catalog number: 140641000) L-Tryptophan (Fisher Scientific, catalog number: BP395) Uracil (Affymetrix, catalog number: 23020) L-Leucine (Acros Organics, catalog number: 125121000) L-Methionine (Fisher Scientific, catalog number: BP388) L-Arginine hydrochloride (Fisher Scientific, catalog number: BP372) L-Serine (Fisher Scientific, catalog number: BP393) Valine (Fisher Scientific, catalog number: BP397) L-Threonine (MP Biomedicals, catalog number: 02103053) L-Isoleucine (Fisher Scientific, catalog number: BP384) L-Phenylalanine (Fisher Scientific, catalog number: BP391) L-Cysteine hydrochloride monohydrate (Fisher Scientific, catalog number: BP376) L-Aspartic Acid (Acros Organics, catalog number: 105041000) L-Proline (Fisher Scientific, catalog number: BP392) Note: Commercially available yeast synthetic drop-out medium supplements (Sigma-Aldrich) can be used instead of a home-made dropout mix .

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  • Team
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  • Contact
  • Bioz Stars
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  • 93
    Valiant yp raffinose
    Yp Raffinose, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yp raffinose/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    yp raffinose - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

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