glassmilk  (Valiant)

 
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    • 93
    Name:
    Glassmilk for ancient DNA extraction
    Description:

    Catalog Number:
    111002202
    Price:
    61.0
    Category:
    Life Sciences Sample Preparation DNA RNA Isolation and Purification Ancient DNA
    Size:
    35 mL
    		Buy from Supplier

    Structured Review

    Valiant glassmilk
    Glassmilk for ancient DNA extraction

    https://www.bioz.com/result/glassmilk/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glassmilk - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar"

    Article Title: Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar

    Journal: Malaria Journal

    doi: 10.1186/s12936-020-3127-x

    Comparison of Ct-values obtained by 4 DNA extraction methods in a P. falciparum dilution row spotted on DBS. Direct pre-PCR (blue), chelex extraction (red), boil and spin extraction followed by glassmilk purification (green) and boil and spin extraction (purple). Experimental replicates are represented by the same symbol
    Figure Legend Snippet: Comparison of Ct-values obtained by 4 DNA extraction methods in a P. falciparum dilution row spotted on DBS. Direct pre-PCR (blue), chelex extraction (red), boil and spin extraction followed by glassmilk purification (green) and boil and spin extraction (purple). Experimental replicates are represented by the same symbol

    Techniques Used: DNA Extraction, Polymerase Chain Reaction, Purification

    Related Articles

    Incubation:

    Article Title: Improved Methods of Carnivore Faecal Sample Preservation, DNA Extraction and Quantification for Accurate Genotyping of Wild Tigers
    Article Snippet: .. 15µl of glassmilk (QBIOGENE) was added to the supernatant and incubated at room temperature for an hour. ..

    Ancient DNA Assay:

    Article Title: Two contemporaneous mitogenomes from terminal Pleistocene burials in eastern Beringia
    Article Snippet: .. The extraction buffer was spun down, and the released DNA molecules in the supernatant were mixed with 4 mL of Guanidine Thiocyanate-based Dehybernation Solution A and 200 µL of Ancient DNA GLASSMILK (silica suspension) components of the GENECLEAN For Ancient DNA Kit (MP Biomedicals), along with a final concentration of 0.05% Tween-20. .. This solution was incubated at 37 °C with rotation for 3 h. The silica particles were collected and purified as per the manufacturer’s protocol for the rest of the GENECLEAN kit.

    Concentration Assay:

    Article Title: Two contemporaneous mitogenomes from terminal Pleistocene burials in eastern Beringia
    Article Snippet: .. The extraction buffer was spun down, and the released DNA molecules in the supernatant were mixed with 4 mL of Guanidine Thiocyanate-based Dehybernation Solution A and 200 µL of Ancient DNA GLASSMILK (silica suspension) components of the GENECLEAN For Ancient DNA Kit (MP Biomedicals), along with a final concentration of 0.05% Tween-20. .. This solution was incubated at 37 °C with rotation for 3 h. The silica particles were collected and purified as per the manufacturer’s protocol for the rest of the GENECLEAN kit.

    Purification:

    Article Title: Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar
    Article Snippet: .. 25 µl of DNA solution was transferred into a new tube. (iii) Boil-and- spin plus glassmilk purification: After boil-and-spin extraction, DNA was purified using glassmilk (MP Biomedicals) according to the supplier’s instructions. (iv) Direct amplification of P. falciparum DNA in pre-PCR (details of final protocol described below) did not require any processing of punches from DBS. .. Four alternative polymerases for direct pre-PCR were tested during assay optimization: Phusion Blood Polymerase (Thermo Fisher Scientific), 2X KAPA HiFi HotStart (KAPA Biosystem), Hemo KlenTaq Polymerase (NEB), MyTaq DNA Polymerase (Bioline).

    Amplification:

    Article Title: Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar
    Article Snippet: .. 25 µl of DNA solution was transferred into a new tube. (iii) Boil-and- spin plus glassmilk purification: After boil-and-spin extraction, DNA was purified using glassmilk (MP Biomedicals) according to the supplier’s instructions. (iv) Direct amplification of P. falciparum DNA in pre-PCR (details of final protocol described below) did not require any processing of punches from DBS. .. Four alternative polymerases for direct pre-PCR were tested during assay optimization: Phusion Blood Polymerase (Thermo Fisher Scientific), 2X KAPA HiFi HotStart (KAPA Biosystem), Hemo KlenTaq Polymerase (NEB), MyTaq DNA Polymerase (Bioline).

    Article Title: DC3, the 21-kDa Subunit of the Outer Dynein Arm-Docking Complex (ODA-DC), Is a Novel EF-Hand Protein Important for Assembly of Both the Outer Arm and the ODA-DC
    Article Snippet: The sequence of clone p25K2–1 was further confirmed by automated sequencing of both strands (DNA Sequencing Facility, Iowa State University, Ames, IA). .. The cDNA insert of p25K2-1 (see above) was amplified by PCR, electrophoresed in an agarose gel, and recovered by excising the gel band and extracting the DNA with NaI and GLASSMILK (Qbiogene, Inc., Carlsbad, CA). .. The DNA was released from the GLASSMILK with 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, and used as template for probe synthesis using the Prime-It II random primer labeling kit.

    Polymerase Chain Reaction:

    Article Title: DC3, the 21-kDa Subunit of the Outer Dynein Arm-Docking Complex (ODA-DC), Is a Novel EF-Hand Protein Important for Assembly of Both the Outer Arm and the ODA-DC
    Article Snippet: The sequence of clone p25K2–1 was further confirmed by automated sequencing of both strands (DNA Sequencing Facility, Iowa State University, Ames, IA). .. The cDNA insert of p25K2-1 (see above) was amplified by PCR, electrophoresed in an agarose gel, and recovered by excising the gel band and extracting the DNA with NaI and GLASSMILK (Qbiogene, Inc., Carlsbad, CA). .. The DNA was released from the GLASSMILK with 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, and used as template for probe synthesis using the Prime-It II random primer labeling kit.

    Agarose Gel Electrophoresis:

    Article Title: DC3, the 21-kDa Subunit of the Outer Dynein Arm-Docking Complex (ODA-DC), Is a Novel EF-Hand Protein Important for Assembly of Both the Outer Arm and the ODA-DC
    Article Snippet: The sequence of clone p25K2–1 was further confirmed by automated sequencing of both strands (DNA Sequencing Facility, Iowa State University, Ames, IA). .. The cDNA insert of p25K2-1 (see above) was amplified by PCR, electrophoresed in an agarose gel, and recovered by excising the gel band and extracting the DNA with NaI and GLASSMILK (Qbiogene, Inc., Carlsbad, CA). .. The DNA was released from the GLASSMILK with 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, and used as template for probe synthesis using the Prime-It II random primer labeling kit.

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    • glassmilk  (Valiant)
    93
    Valiant glassmilk
    Comparison of Ct-values obtained by 4 DNA extraction methods in a P. falciparum dilution row spotted on DBS. Direct pre-PCR (blue), chelex extraction (red), boil and spin extraction followed by <t>glassmilk</t> purification (green) and boil and spin extraction (purple). Experimental replicates are represented by the same symbol
    Glassmilk, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glassmilk/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glassmilk - by Bioz Stars, 2021-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of Ct-values obtained by 4 DNA extraction methods in a P. falciparum dilution row spotted on DBS. Direct pre-PCR (blue), chelex extraction (red), boil and spin extraction followed by glassmilk purification (green) and boil and spin extraction (purple). Experimental replicates are represented by the same symbol

    Journal: Malaria Journal

    Article Title: Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar

    doi: 10.1186/s12936-020-3127-x

    Figure Lengend Snippet: Comparison of Ct-values obtained by 4 DNA extraction methods in a P. falciparum dilution row spotted on DBS. Direct pre-PCR (blue), chelex extraction (red), boil and spin extraction followed by glassmilk purification (green) and boil and spin extraction (purple). Experimental replicates are represented by the same symbol

    Article Snippet: 25 µl of DNA solution was transferred into a new tube. (iii) Boil-and- spin plus glassmilk purification: After boil-and-spin extraction, DNA was purified using glassmilk (MP Biomedicals) according to the supplier’s instructions. (iv) Direct amplification of P. falciparum DNA in pre-PCR (details of final protocol described below) did not require any processing of punches from DBS.

    Techniques: DNA Extraction, Polymerase Chain Reaction, Purification