buffer a  (Valiant)

 
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    Name:
    NEW Wash Buffer concentrate
    Description:
    NEW Wash Concentrate is a concentrated solution of NaCl Tris and EDTA to which water and ethanol are added to make NEW Wash buffer which is used to purify DNA
    Catalog Number:
    111001202-cf
    Price:
    10.0
    Category:
    Life Sciences Sample Preparation DNA RNA Isolation and Purification Genomic DNA Isolation
    Applications:
    Purification of DNA
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    Structured Review

    Valiant buffer a
    NEW Wash Buffer concentrate
    NEW Wash Concentrate is a concentrated solution of NaCl Tris and EDTA to which water and ethanol are added to make NEW Wash buffer which is used to purify DNA
    https://www.bioz.com/result/buffer a/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer a - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Activity Assay:

    Article Title: The Trypanosoma brucei dihydroxyacetonephosphate acyltransferase TbDAT is dispensable for normal growth but important for synthesis of ether glycerophospholipids
    Article Snippet: DHAPAT and GPAT activity assays were performed as described in [ , , ]. .. Briefly, GPAT assay was performed in a buffer system containing 20 mM TrisHCl pH7.5, 4 mM NaF, 1 mM DTT, 2 mM MgCl2 , 1 mg/ml fatty acid free BSA, 100 μM fatty acyl-CoA, 0.4 mM G3P (including 5.7 μM of [U14 C]L-G3P; specific activity of 25 mCi/mmol; MP Biomedicals, LLC, Santa Ana, CA), and 200 μg of protein extract in a total volume of 200 μl. .. The reaction was incubated at 30°C and stopped after10 min with 700 μl of 1% HClO4 .

    Stripping Membranes:

    Article Title: Autoimmunity against INS-IGF2 Protein Expressed in Human Pancreatic Islets *Autoimmunity against INS-IGF2 Protein Expressed in Human Pancreatic Islets * ♦
    Article Snippet: A total of 50 μl of reaction mixture was then incubated for 1 h at 4 °C with 50 μl of protein A-Sepharose 4B conjugated (20%, washed four times at 4 °C by sedimentation in antigen buffer) (Invitrogen) in a 96-well filtration plate (MultiScreenHTS -DV Plate, Millipore AB, Solna, Sweden). .. The plate was then washed eight times with wash buffer (150 m m NaCl, 20 m m Tris, 0.15% Tween 20 (MP Biomedicals, LLC), pH 7.4) using a microplate washer (ELx50TM Microplate Strip Washer, Biotek Instruments, Inc., Winooski, VT). .. Antibody-bound radioactivity was counted in a β-counter (1450 MicroBeta® TriLux, PerkinElmer Life Sciences).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Lipoprotein Lipase SNPs rs13702 and rs301 Correlate with Clinical Outcome in Chronic Lymphocytic Leukemia Patients
    Article Snippet: All qPCR reactions were performed in duplicate on a LightCycler 480 (Roche Diagnostics). .. LPL ELISA To determine LPL protein levels in 44 samples, patient PBMCs were lysed using a 2× concentrated lysis buffer composed of 0.25% Triton X-100 (MP Biomedicals, Solon, Ohio, USA), 50 mM KCL, 100 mM TrisHCL pH 7.4, 40% glycerol [ ]. ..

    Lysis:

    Article Title: Lipoprotein Lipase SNPs rs13702 and rs301 Correlate with Clinical Outcome in Chronic Lymphocytic Leukemia Patients
    Article Snippet: All qPCR reactions were performed in duplicate on a LightCycler 480 (Roche Diagnostics). .. LPL ELISA To determine LPL protein levels in 44 samples, patient PBMCs were lysed using a 2× concentrated lysis buffer composed of 0.25% Triton X-100 (MP Biomedicals, Solon, Ohio, USA), 50 mM KCL, 100 mM TrisHCL pH 7.4, 40% glycerol [ ]. ..

    Blocking Assay:

    Article Title: A C. elegans eIF4E-family member upregulates translation at elevated temperatures of mRNAs encoding MSH-5 and other meiotic crossover proteins
    Article Snippet: Proteins were transferred to PVDF membranes (Bio-Rad) in a Mini Trans-Blot cell (Bio-Rad) at 90 V for 2 hours at 4°C. .. Membranes were blocked in buffer A [20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05% (v/v) Tween-20] containing 5% Phosphoblocker Blocking Reagent (Cell Biolabs, Inc.), washed four times (30 minutes each) with buffer A, and incubated in buffer A containing 5% Phosphoblocker and the primary antibody overnight at 4°C [1:500 dilution for anti-MSH-5 and 1:10,000 for mouse anti-β-actin (C4) (MP Biomedicals)]. .. Membranes were then washed four times (30 minutes each) with buffer A and incubated in buffer A containing 5% Phosphoblocker and a 1:10,000 dilution of either peroxidase-labeled goat anti-rabbit IgG (H+L) or horse anti-mouse IgG (H+L; Vector Laboratories) for 70 minutes at room temperature.

    Article Title: Three Dimensional Cellular Microarray Platform for Human Neural Stem Cell Differentiation and Toxicology
    Article Snippet: The cells were then permeabilized for 10 min with 0.15% (v/v) Triton X-100 (Sigma), rinsed three times with the wash buffer, and incubated overnight at 4°C with a blocking solution (TBS Superblock from Pierce). .. After blocking, the chips were rinsed three times with wash buffer containing 0.1% (v/v) Tween-20 (MP Biomedicals) and incubated for 2 h at room temperature with the various primary antibodies diluted in 1% (w/v) bovine serum albumin (BSA, Santa Cruz Biotechnology) in the wash buffer. .. The antibodies used were rabbit polyclonal anti-GalC (1:250 dilution, Santa Cruz Biotechnology), mouse monoclonal anti-GAPDH (1:100 dilution, Abcam), mouse monoclonal anti-GFAP (1:100 dilution, Abcam), mouse monoclonal anti-Nestin (1:100 dilution, BD BioScience), mouse monoclonal anti-βIII-Tubulin (1:400 dilution, Covance), and rabbit polyclonal anti-γ-Tubulin (1:1000 dilution, Sigma-Aldrich).

    Incubation:

    Article Title: A C. elegans eIF4E-family member upregulates translation at elevated temperatures of mRNAs encoding MSH-5 and other meiotic crossover proteins
    Article Snippet: Proteins were transferred to PVDF membranes (Bio-Rad) in a Mini Trans-Blot cell (Bio-Rad) at 90 V for 2 hours at 4°C. .. Membranes were blocked in buffer A [20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05% (v/v) Tween-20] containing 5% Phosphoblocker Blocking Reagent (Cell Biolabs, Inc.), washed four times (30 minutes each) with buffer A, and incubated in buffer A containing 5% Phosphoblocker and the primary antibody overnight at 4°C [1:500 dilution for anti-MSH-5 and 1:10,000 for mouse anti-β-actin (C4) (MP Biomedicals)]. .. Membranes were then washed four times (30 minutes each) with buffer A and incubated in buffer A containing 5% Phosphoblocker and a 1:10,000 dilution of either peroxidase-labeled goat anti-rabbit IgG (H+L) or horse anti-mouse IgG (H+L; Vector Laboratories) for 70 minutes at room temperature.

    Article Title: Three Dimensional Cellular Microarray Platform for Human Neural Stem Cell Differentiation and Toxicology
    Article Snippet: The cells were then permeabilized for 10 min with 0.15% (v/v) Triton X-100 (Sigma), rinsed three times with the wash buffer, and incubated overnight at 4°C with a blocking solution (TBS Superblock from Pierce). .. After blocking, the chips were rinsed three times with wash buffer containing 0.1% (v/v) Tween-20 (MP Biomedicals) and incubated for 2 h at room temperature with the various primary antibodies diluted in 1% (w/v) bovine serum albumin (BSA, Santa Cruz Biotechnology) in the wash buffer. .. The antibodies used were rabbit polyclonal anti-GalC (1:250 dilution, Santa Cruz Biotechnology), mouse monoclonal anti-GAPDH (1:100 dilution, Abcam), mouse monoclonal anti-GFAP (1:100 dilution, Abcam), mouse monoclonal anti-Nestin (1:100 dilution, BD BioScience), mouse monoclonal anti-βIII-Tubulin (1:400 dilution, Covance), and rabbit polyclonal anti-γ-Tubulin (1:1000 dilution, Sigma-Aldrich).

    Article Title: High-affinity agonist binding to C5aR results from a cooperative two-site binding mechanism
    Article Snippet: After additional 15 min incubation at 25 °C, the reaction was terminated by adding 5 ml of cold wash buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl and 5mM MgCl2 , and filtering through glass fiber filters (Millipore Sigma). .. After washing the filters twice with 5 ml cold wash buffer, the filters were incubated with 5 ml of CytoScint liquid scintillation cocktail (MP Biomedicals). .. The radiation of bound 35 S-GTPγS was measured on a Beckman LS6500 scintillation counter to determine the binding of 35 S-GTPγS to Gi induced by C5aR activation.

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  • 93
    Valiant buffer a
    Buffer A, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer a/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer a - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

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