1x pcr buffer (Solis BioDyne)

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Solis BioDyne 1x pcr buffer
1x Pcr Buffer, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x pcr buffer/product/Solis BioDyne
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

1x pcr buffer - by Bioz Stars, 2021-09

86/100 stars

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Polymerase Chain Reaction:

Article Title: Limited added value of fungal ITS amplicon sequencing in the study of bovine abortion
Article Snippet: .. PCR was performed in 30 μL reaction mixtures containing 1x PCR buffer, 2 mM MgCl2 , 0.4 μM forward and reverse primer, 200 μM deoxyribonucleotide triphosphates (dNTPs), 0.25 μL of 5 U/μL thermostable DNA FIREPol® Polymerase Solis BioDyne, 21.25 μL of sterile water and 2 μL of DNA solution. ..

Article Title: A dual colour FISH method for routine validation of sexed Bos taurus semen
Article Snippet: .. The 20 μL reaction mix consisted of 1X PCR buffer (Solis BioDyne), 3 mM MgCl2 , 600 μM of each dATP, dCTP, dGTP, 400 μM of dTTP, 200 μM of aa-dUTP (Thermo Scientific), 500 nM of the each five forward and the five reverse primers (Metabion), 10 ng of the pooled DNA sequences from the first PCR and 1.5 U of FIREPol® DNA polymerase (Solis BioDyne). ..

Article Title: Rapid in vitro detection of CTX-M groups 1, 2, 8, 9 resistance genes by LAMP assays
Article Snippet: .. The amplification was carried out in a 25μl total reaction volume with 2μl genomic DNA as template, 1X PCR Solys Biodyne Buffer (20mMTris-HCl, 10mMKCl, 2mM MgSO4 , pH 8.8), 0.6M of betaine, 0.5μl (10μM) of a couple of appropriate primers (CTX-M group-F/CTX-M group-R), 0.5μl of dNTPs mixture (2.5mM of each dNTPs), and 0.125μl (5U/ml) of Taq DNA polymerase (Solis Biodyne). ..

Article Title: SNaPshot Assay for the Detection of the Most Common CFTR Mutations in Infertile Men
Article Snippet: .. PCR conditions and capillary electrophoresis PCR reaction mix contained 1X reaction buffer B2, 2.5 mM MgCl2 , 200 µM each dNTP, 100 nM each primer, 1X Solution S, 1 U HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 100 ng genomic DNA. ..

Article Title: A dual colour FISH method for routine validation of sexed Bos taurus semen
Article Snippet: .. The PCR amplification of all the X chromosome-specific DNA sequences was carried out in 20 μL reactions consisting of 1X PCR buffer (Solis BioDyne, Tartu, Estonia), 1.5 mM MgCl2 , 300 μM of each nucleotide (Thermo Scientific, Waltham, MA, USA), 500 nM of each forward and reverse primers (Metabion, Steinkirchen, Germany), 50 ng of bovine genomic DNA and 1.5 U of FIREPol® DNA polymerase (Solis BioDyne). ..

Quantitative RT-PCR:

Article Title: Expression of Progesterone Receptor Membrane Component 1 (PGRMC1), Progestin and AdipoQ Receptor 7 (PAQPR7), and Plasminogen Activator Inhibitor 1 RNA-Binding Protein (PAIRBP1) in Glioma Spheroids In Vitro
Article Snippet: .. RT-qPCR reactions were done in 20 μ L mixes including 12.5 ng cDNA template, 0.2 mM of each dNTP, 3 mM MgCl2 , 1x buffer B2 (Solis BioDyne, Tartu, Estonia), 300 nM of each primer, 200 nM probe, 50 nM ROX reference dye (Biotium, Hayward, USA), and 1-unit HOT FIREPol DNA polymerase (Solis BioDyne). ..

Amplification:

Article Title: Beyond the Binding Site: In Vivo Identification of tbx2, smarca5 and wnt5b as Molecular Targets of CNBP during Embryonic Development
Article Snippet: .. In the case of tbx2b (t 2), 5% DMSO was added to reaction medium, while wnt5b (w 1) and wnt5b (w2) amplification required HOTFIREpol polymerase plus 1X S solution (Solis Biodine). ..

1x pcr buffer (Solis BioDyne)

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