10x pcr buffer  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Roche 10x pcr buffer
    10x Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x pcr buffer/product/Roche
    Average 92 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    10x pcr buffer - by Bioz Stars, 2020-09
    92/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Risk for HIV-1 infection is not associated with Repeat-Region polymorphism in the DC-SIGN neck domain and Novel Genetic DC-SIGN Variants among North Indians
    Article Snippet: .. Polymerase Chain Reaction (PCR) was conducted in a 25 µl reaction containing 50 ng of genomic DNA, 5 pmol of each primer, 175 µM dNTPs, 1.5 mM MgCl2 , 10X PCR buffer and 0.5 U of Taq polymerase (Roche). .. Thermocycling procedure (PerkinElmer 9600) consisted of initial denaturation at 94 °C for 4 min followed by 35 cycles of 94 °C for 30 s, 52 °C for 45 s and 72 °C for 1 min and final extension of 72 °C for 7 min. Amplicons were visualized by ultraviolet transillumination in 2% agarose gel containing ethidium bromide.

    Article Title: Global radiation in a rare biosphere soil diatom
    Article Snippet: .. The PCR mixtures contained per sample 1 µL of the template DNA, 0.4 µM of each primer, 200 µM of each deoxynucleotide triphosphate, 2.5 µL of 10x PCR buffer, and 0.25U of Fast Start High fidelity Taq polymerase (Roche Inc.). .. The final reaction volume was adjusted to 25 µL using HPLC water.

    Article Title: Real-Time Quantitative PCR of Microdissected Paraffin-Embedded Breast Carcinoma
    Article Snippet: .. Reverse transcription (RT) took place in a final reaction volume of 20 μl containing 50 units MuLV reverse transcriptase (Applied Biosystems, Foster City, CA), 10X PCR buffer, 5 pmol specific antisense primer (also used in PCR) for either Her-2/ neu or β-actin, 4 U RNase inhibitor (Roche), 4 mmol/L of each dNTPs, 6.25 mmol/L MgCl2 , and 2 μl template. .. The RT step was performed on a Perkin Elmer 9700 Thermocycler at 42°C for 30 minutes followed by 94°C for 5 minutes.

    Article Title: Evaluation of immunogenicity and protective effect of DNA vaccine encoding surface antigen1 (SAG1) of Toxoplasma gondii and TLR-5 ligand as a genetic adjuvant against acute toxoplasmosis in BALB/c mice.
    Article Snippet: .. Toxoplasma gondii is an obligate intracellular, protozoan that causes a high incidence of serious zoonotic parasitic disease in humans. .. Toxoplasma gondii is an obligate intracellular, protozoan that causes a high incidence of serious zoonotic parasitic disease in humans.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Roche pcr buffer
    Antigenic region of the VacA gene from Helicobacter pylori amplified by <t>PCR.</t> Lane 1: <t>DNA</t> marker, Lane 2: PCR product
    Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 905 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Roche
    Average 94 stars, based on 905 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    85
    Roche 10x faststart taq dna polymerase pcr buffer
    Antigenic region of the VacA gene from Helicobacter pylori amplified by <t>PCR.</t> Lane 1: <t>DNA</t> marker, Lane 2: PCR product
    10x Faststart Taq Dna Polymerase Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x faststart taq dna polymerase pcr buffer/product/Roche
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    10x faststart taq dna polymerase pcr buffer - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Antigenic region of the VacA gene from Helicobacter pylori amplified by PCR. Lane 1: DNA marker, Lane 2: PCR product

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Expression and Antigenic Evaluation of VacA Antigenic Fragment of Helicobacter Pylori

    doi:

    Figure Lengend Snippet: Antigenic region of the VacA gene from Helicobacter pylori amplified by PCR. Lane 1: DNA marker, Lane 2: PCR product

    Article Snippet: PCR amplification was performed in a 50 μl total volume containing 3 μl of template DNA, 2 μl of each primers (10 picomole), 8 μl MgCl₂ (25 mM), 1.5 μl dNTP mixture, 5 μl PCR buffer (10X) and 1 μl of expand DNA polymerase (Roche, Germany).

    Techniques: Amplification, Polymerase Chain Reaction, Marker

    Colony PCR showing the cDNA derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).

    Journal: PLoS ONE

    Article Title: A New Component of the Nasonia Sex Determining Cascade Is Maternally Silenced and Regulates Transformer Expression

    doi: 10.1371/journal.pone.0063618

    Figure Lengend Snippet: Colony PCR showing the cDNA derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).

    Article Snippet: One l 1∶100 diluted cDNA was mixed with 10x PCR buffer (Roche) (10x concentrate; 100 mM Tris-HCl, 500 mM KCl; pH 8.3), 200 M dNTPs, 2 units Taq polymerase (Roche), 400 nM of primers NvTra_poly_F1 and NvTra_poly_R1 and supplemented with milliQ water to 25 l. PCR profile was as follows: initial denaturing step of 95 C for 3 min, followed by 20 cycles of 95 C for 15 s, 57 C for 30 s and 72 C for 30 s, ending with a final extension step of 72 C for 7 min. As negative control one l of milliQ water was used.

    Techniques: Polymerase Chain Reaction, Derivative Assay

    Electrophoresis of PCR product of Sarcocystis isolated from goat and calf in northern Iran. Lanes: 1 2, Sarcocystis isolated from goat; 3, Sarcocystis isolated from calf muscle sample; M, 100 bp ladder DNA size marker; and N, negative control

    Journal: International Journal of Molecular and Cellular Medicine

    Article Title: Sarcocystis cruzi: First Molecular Identification from Cattle in Iran

    doi:

    Figure Lengend Snippet: Electrophoresis of PCR product of Sarcocystis isolated from goat and calf in northern Iran. Lanes: 1 2, Sarcocystis isolated from goat; 3, Sarcocystis isolated from calf muscle sample; M, 100 bp ladder DNA size marker; and N, negative control

    Article Snippet: One reaction mixture contained 3 µl of the DNA solution, 20 µl of dH2 O, 3 µl of 10x PCR buffer (Roche, Germany), 0.5 µl of dNTP (Roche, Germany), 0.5 µl of Tag polymrase (Roche, Germany), 0.5 µl of each primer (20 pmol), 0.5 µl of MgCl 2 and RNase-free water to make a final volume of 30 µl.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Isolation, Northern Blot, Marker, Negative Control