10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1 - by Bioz Stars, 2023-12
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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1 - by Bioz Stars, 2023-12
    86/100 stars

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    1) Product Images from "Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a"

    Article Title: Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a

    Journal: bioRxiv

    doi: 10.1101/2023.11.14.567134

    (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.
    Figure Legend Snippet: (a) The concentration of Cas enzyme and crRNA, together referred to as ribonucleoprotein (RNP) was tuned for most sensitive detection of AAV genomic titer. 4 RNP concentrations were tested against AAV genome titers ranging from 1e8 to 5e9 vg/mL. 10 nM RNP was selected was the most robust assay condition. Next, the standard NEBuffer 2.1, typically containing 10 mM Mg 2+ was custom prepared to test the effects of modifying magnesium concentration. Results after (b) 30 minutes and (c) 60 minutes of reaction show that increasing the Magnesium above 10 mM provide additional sensitivity represented by signal fold change over the NTC, where error bars represent standard deviation (n = 3). Statistical analysis was performed using a two tailed student’s t-test, where ns = not significant with p > 0.05, and asterisks (* = p < 0.005, ** = p < 0.0005) represent significant differences. (d) Buffer composition effect on CRAAVE assay sensitivity was further assessed by modifying both NaCl and MgCl 2 concentrations. Significantly better sensitivity was achieved when total ionic strength was lowered, but magnesium concentration was kept at 20 mM.

    Techniques Used: Concentration Assay, Standard Deviation, Two Tailed Test

    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1  (New England Biolabs)


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    10x nebuffer 2 1  (New England Biolabs)


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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1
    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    10x nebuffer 2 1  (New England Biolabs)


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    New England Biolabs 10x nebuffer 2 1

    10x Nebuffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Cas12a ortholog with distinct TTNA PAM enables sensitive detection of HPV16/18"

    Article Title: A Cas12a ortholog with distinct TTNA PAM enables sensitive detection of HPV16/18

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2023.100444


    Figure Legend Snippet:

    Techniques Used: Recombinant, Isolation, Modification, Transfection, SYBR Green Assay, Gel Extraction, Amplification, Stripping Membranes, DNA Extraction, Sequencing, RNA Sequencing Assay, Software

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