10x concentration  (Millipore)

 
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  • 95
    Name:
    Tris Phosphate EDTA Buffer 10X
    Description:

    Catalog Number:
    T3154
    Price:
    None
    Applications:
    Tris-Phosphate-EDTA Buffer 10X is diluted to working concentration and used for gel electrophoresis and gene expression studies.
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    Structured Review

    Millipore 10x concentration
    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, <t>10X</t> Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    https://www.bioz.com/result/10x concentration/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x concentration - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity"

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.01784

    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value
    Figure Legend Snippet: C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Techniques Used: Derivative Assay, Western Blot, Software, Zymography, Activity Assay, Cell Culture, Quantitation Assay

    2) Product Images from "Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity"

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.01784

    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value
    Figure Legend Snippet: C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Techniques Used: Derivative Assay, Western Blot, Software, Zymography, Activity Assay, Cell Culture, Quantitation Assay

    Related Articles

    Concentration Assay:

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity
    Article Snippet: After 5 h of incubation at 37°C, Ctrl- C2C12-, or P3F-C2C12-derived exosomes (at 10X concentration) were added to the cells. .. Then 40 h later, cells were treated simultaneously with 60 μM H2 O2 and Ctrl-C2C12 or P3F-C2C12 exosomes at 10X concentration. .. Cells were counted using trypan blue (Sigma) at 8 and 24 h following H2 O2 treatment.

    Article Title: A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression
    Article Snippet: After digestion was stopped, the soluble chromatin fraction (SN1), which accounted for ~66% of total chromatin, was prepared by centrifugation at 10,000×g for 15 min at 4 °C. .. The remaining insoluble material was extracted at increasing EDTA concentration from 2 to 200 mM. .. Nucleosomal composition of each fraction was analyzed by agarose gel electrophoresis (Supplementary Fig. ).

    Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution
    Article Snippet: Following optimization, where a bulk digestion method of one step full-tissue digestion using umbilical cord digestion kit (Milteyni Biotech) of all tissue as per manufacturers instructions, the optimum method for digestion was identified for the rest of the atlas. .. This consisted of a crypt chelation protocol as previously described ( ) with a modification of increasing EDTA concentration in digestion media to 5mM and for two digestion steps of 20 minute in a waterbath at 37°C, with agitation and collection of the supernatant after each incubation. .. The isolated epithelial crypts in the supernatant were processed to a single cell suspension with TypLE Express (GIBCO) and DNase 50μg/ml (Sigma) for 45 minutes while the remaining crypt-depleted tissue was digested with Umbilical Cord Dissociation kit (Milteyni Biotec) at 37°C with regular agitation using a blunt needle and syringe.

    Article Title: Breast cancer patient‐derived scaffolds as a tool to monitor chemotherapy responses in human tumor microenvironments, et al. Breast cancer patient‐derived scaffolds as a tool to monitor chemotherapy responses in human tumor microenvironments
    Article Snippet: .. After that, 3DPS were treated with 5‐FU and DOX at the 10X concentration as the PDS treatments. .. For RNA extraction, 3DPS were washed twice in cell media, lysed in 700 µl QIAzol (Qiagen) and homogenized for 2 × 2.5 min at 25 Hz.

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity
    Article Snippet: Cells were counted using trypan blue (Sigma) at 8 and 24 h following H2 O2 treatment. .. Flow Cytometry for ROS Production Evaluation Reactive oxygen species levels were evaluated for Ctrl-C2C12 and P3F-C2C12, or C2C12 cells treated with Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes at a 10X concentration. .. In summary, 30,000 cells were seeded in 6-well plates, and incubated at 37°C for 48 h. When needed, exosomes were added 5 h post plating.

    Article Title: Identification of annotated bioactive molecules that impair motility of the blood fluke Schistosoma mansoni
    Article Snippet: In place of the media, 100 μL HBSS supplemented with 0.1% bovine serum albumin (BSA), 500 μM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich), 20 mM HEPES (pH 7.4) and 1 mg/mL D-luciferin (Goldbio) was added to each well. .. After a 2 h incubation period at room temperature, luminescence background was recorded, and compounds and control ligands were added at 10X concentration. .. Immediately after compound addition, luminescence was read over a 45 min period to detect compounds stimulating cAMP release through receptor activation.

    Modification:

    Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution
    Article Snippet: Following optimization, where a bulk digestion method of one step full-tissue digestion using umbilical cord digestion kit (Milteyni Biotech) of all tissue as per manufacturers instructions, the optimum method for digestion was identified for the rest of the atlas. .. This consisted of a crypt chelation protocol as previously described ( ) with a modification of increasing EDTA concentration in digestion media to 5mM and for two digestion steps of 20 minute in a waterbath at 37°C, with agitation and collection of the supernatant after each incubation. .. The isolated epithelial crypts in the supernatant were processed to a single cell suspension with TypLE Express (GIBCO) and DNase 50μg/ml (Sigma) for 45 minutes while the remaining crypt-depleted tissue was digested with Umbilical Cord Dissociation kit (Milteyni Biotec) at 37°C with regular agitation using a blunt needle and syringe.

    Incubation:

    Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution
    Article Snippet: Following optimization, where a bulk digestion method of one step full-tissue digestion using umbilical cord digestion kit (Milteyni Biotech) of all tissue as per manufacturers instructions, the optimum method for digestion was identified for the rest of the atlas. .. This consisted of a crypt chelation protocol as previously described ( ) with a modification of increasing EDTA concentration in digestion media to 5mM and for two digestion steps of 20 minute in a waterbath at 37°C, with agitation and collection of the supernatant after each incubation. .. The isolated epithelial crypts in the supernatant were processed to a single cell suspension with TypLE Express (GIBCO) and DNase 50μg/ml (Sigma) for 45 minutes while the remaining crypt-depleted tissue was digested with Umbilical Cord Dissociation kit (Milteyni Biotec) at 37°C with regular agitation using a blunt needle and syringe.

    Article Title: Identification of annotated bioactive molecules that impair motility of the blood fluke Schistosoma mansoni
    Article Snippet: In place of the media, 100 μL HBSS supplemented with 0.1% bovine serum albumin (BSA), 500 μM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich), 20 mM HEPES (pH 7.4) and 1 mg/mL D-luciferin (Goldbio) was added to each well. .. After a 2 h incubation period at room temperature, luminescence background was recorded, and compounds and control ligands were added at 10X concentration. .. Immediately after compound addition, luminescence was read over a 45 min period to detect compounds stimulating cAMP release through receptor activation.

    other:

    Article Title: Role of Mitochondria and Lysosomes in the Selective Cytotoxicity of Cold Atmospheric Plasma on Retinoblastoma Cells
    Article Snippet: One-hundred microliter of diluted supernatant was added to 2.8 mL phosphate-EDTA buffer and 100 µL of the OPT solution.

    Flow Cytometry:

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity
    Article Snippet: Cells were counted using trypan blue (Sigma) at 8 and 24 h following H2 O2 treatment. .. Flow Cytometry for ROS Production Evaluation Reactive oxygen species levels were evaluated for Ctrl-C2C12 and P3F-C2C12, or C2C12 cells treated with Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes at a 10X concentration. .. In summary, 30,000 cells were seeded in 6-well plates, and incubated at 37°C for 48 h. When needed, exosomes were added 5 h post plating.

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  • 95
    Millipore 10x concentration
    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, <t>10X</t> Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value
    10x Concentration, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x concentration/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x concentration - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    94
    Millipore 10x concentrated
    (P)RR in urine samples from NS and LS Sprague-Dawley rats. A. 24 hour-urine collections were <t>10X</t> concentrated and 40 micrograms loaded in each well (n=4). Samples were incubated with specific (P)RR antibody. As loading control, unspecific staining of major urinary proteins is also shown in the same membrane. B. Quantification of specific (P)RR immunoblots normalized by total protein (as percentage of NS rats, * P
    10x Concentrated, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x concentrated/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x concentrated - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    95
    Millipore final 10x concentration
    LZD and LZD_IL-1Rn reduce inflammatory signatures in BAL. Bronchoalveolar lavages (BAL) were acquired pre-treatment and 3 weeks post-treatment with LZD (blue) or LZD+IL-1Rn (red). (A) Cells were analyzed by flow cytometry to determine frequency changes in CD4 T cells (CD3+CD4+), CD8 T cells (CD3+CD8+), macrophages (CD11b+CD206+), and neutrophils (CD11b+Calprotectin+). Wilcoxon signed rank test was performed to determine differences before and after drug treatment, regardless of group (LZD and LZD+IL-1Rn combined). (B) BAL fluid was concentrated <t>10X</t> and assessed by multiplex assay for changes in inflammatory cytokines and chemokines for a random subset of samples ( n = 3 per treatment group). Wilcoxon signed rank test was performed to determine differences before and after drug treatment, regardless of group (LZD and LZD+IL-1Rn combined).
    Final 10x Concentration, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/final 10x concentration/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    final 10x concentration - by Bioz Stars, 2021-06
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    94
    Millipore permethrin
    <t>Permethrin</t> resistance intensity in Anopheles gambiae , An . coluzzii and An . arabiensis from Lagos (LA), Ogun (OG), Edo (ED), Anambra (AN), Niger (NG) and Kwara (KW).
    Permethrin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/permethrin/product/Millipore
    Average 94 stars, based on 1 article reviews
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    permethrin - by Bioz Stars, 2021-06
    94/100 stars
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    Image Search Results


    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Journal: Frontiers in Oncology

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity

    doi: 10.3389/fonc.2020.01784

    Figure Lengend Snippet: C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Article Snippet: Then 40 h later, cells were treated simultaneously with 60 μM H2 O2 and Ctrl-C2C12 or P3F-C2C12 exosomes at 10X concentration.

    Techniques: Derivative Assay, Western Blot, Software, Zymography, Activity Assay, Cell Culture, Quantitation Assay

    (P)RR in urine samples from NS and LS Sprague-Dawley rats. A. 24 hour-urine collections were 10X concentrated and 40 micrograms loaded in each well (n=4). Samples were incubated with specific (P)RR antibody. As loading control, unspecific staining of major urinary proteins is also shown in the same membrane. B. Quantification of specific (P)RR immunoblots normalized by total protein (as percentage of NS rats, * P

    Journal: The American journal of the medical sciences

    Article Title: Angiotensin II increases the expression of (pro)renin receptor during low salt conditions in rat renal inner medullary collecting ducts

    doi: 10.1097/MAJ.0000000000000335

    Figure Lengend Snippet: (P)RR in urine samples from NS and LS Sprague-Dawley rats. A. 24 hour-urine collections were 10X concentrated and 40 micrograms loaded in each well (n=4). Samples were incubated with specific (P)RR antibody. As loading control, unspecific staining of major urinary proteins is also shown in the same membrane. B. Quantification of specific (P)RR immunoblots normalized by total protein (as percentage of NS rats, * P

    Article Snippet: Urine collections (24 hrs) from NS and LS were 10X concentrated and subsequently subjected to Western blot analysis (40 micrograms of total protein) using a polyclonal rabbit anti-(P)RR that recognizes the intracellular segment and the ecto-domain (ATP6AP2, 1:200 dilution; Sigma-Aldrich) as described previously.

    Techniques: Incubation, Staining, Western Blot

    LZD and LZD_IL-1Rn reduce inflammatory signatures in BAL. Bronchoalveolar lavages (BAL) were acquired pre-treatment and 3 weeks post-treatment with LZD (blue) or LZD+IL-1Rn (red). (A) Cells were analyzed by flow cytometry to determine frequency changes in CD4 T cells (CD3+CD4+), CD8 T cells (CD3+CD8+), macrophages (CD11b+CD206+), and neutrophils (CD11b+Calprotectin+). Wilcoxon signed rank test was performed to determine differences before and after drug treatment, regardless of group (LZD and LZD+IL-1Rn combined). (B) BAL fluid was concentrated 10X and assessed by multiplex assay for changes in inflammatory cytokines and chemokines for a random subset of samples ( n = 3 per treatment group). Wilcoxon signed rank test was performed to determine differences before and after drug treatment, regardless of group (LZD and LZD+IL-1Rn combined).

    Journal: Frontiers in Immunology

    Article Title: Evaluation of IL-1 Blockade as an Adjunct to Linezolid Therapy for Tuberculosis in Mice and Macaques

    doi: 10.3389/fimmu.2020.00891

    Figure Lengend Snippet: LZD and LZD_IL-1Rn reduce inflammatory signatures in BAL. Bronchoalveolar lavages (BAL) were acquired pre-treatment and 3 weeks post-treatment with LZD (blue) or LZD+IL-1Rn (red). (A) Cells were analyzed by flow cytometry to determine frequency changes in CD4 T cells (CD3+CD4+), CD8 T cells (CD3+CD8+), macrophages (CD11b+CD206+), and neutrophils (CD11b+Calprotectin+). Wilcoxon signed rank test was performed to determine differences before and after drug treatment, regardless of group (LZD and LZD+IL-1Rn combined). (B) BAL fluid was concentrated 10X and assessed by multiplex assay for changes in inflammatory cytokines and chemokines for a random subset of samples ( n = 3 per treatment group). Wilcoxon signed rank test was performed to determine differences before and after drug treatment, regardless of group (LZD and LZD+IL-1Rn combined).

    Article Snippet: For BAL samples, supernatants were concentrated using regenerated cellulose centrifugal filter tubes (3,000 NMWL, Millipore Sigma) to a final 10X concentration (5 to 0.5 mL).

    Techniques: Flow Cytometry, Multiplex Assay

    Permethrin resistance intensity in Anopheles gambiae , An . coluzzii and An . arabiensis from Lagos (LA), Ogun (OG), Edo (ED), Anambra (AN), Niger (NG) and Kwara (KW).

    Journal: PLoS ONE

    Article Title: Pyrethroids resistance intensity and resistance mechanisms in Anopheles gambiae from malaria vector surveillance sites in Nigeria

    doi: 10.1371/journal.pone.0205230

    Figure Lengend Snippet: Permethrin resistance intensity in Anopheles gambiae , An . coluzzii and An . arabiensis from Lagos (LA), Ogun (OG), Edo (ED), Anambra (AN), Niger (NG) and Kwara (KW).

    Article Snippet: The 5x and 10x diagnostic concentrations were prepared using Technical-grade of permethrin and deltamethrin (Sigma-Aldrich) diluted in acetone and olive oil (1:1) as contained in the manufactural instruction to form a stock solution.

    Techniques: