Journal: Engineering in Life Sciences

Article Title: Automation of a Capillary‐Wave Microbioreactor Platform to Enhance Phage Sensitivity Screen Efficiency

doi: 10.1002/elsc.70021

Figure Lengend Snippet: Cell number of E. coli during a phagogram in the automated cwMBR platform with phage MM02 (MOI of 10 −3 and 10 −5 ) at 37°C in complex medium (oscillation frequency of the cwMBR 70 Hz, amplitude 5%). The graphs show the mean and the standard deviation (lightly colored) from triplicates. Automated infection using the nanodispenser with the phages was performed at t = 1 h of incubation of the culture in the cwMBR without phages. The second nanodispenser was applied to compensate for evaporation by adding deionized water every 15 min beginning after 15 min of cultivation.

Article Snippet: Therefore, E. coli K12 MG1655 (DSM 18039) and the phage vB_EcoM_MM02 (short: MM02; DSM 29475) were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and used as bacterium and model phage, respectively.

Techniques: Standard Deviation, Infection, Incubation, Evaporation