Journal: Vaccines

Article Title: Rotavirus A Infection Prevalence and Spatio-Temporal Genotype Shift among Under-Five Children in Amhara National Regional State, Ethiopia: A Multi-Center Cross-Sectional Study

doi: 10.3390/vaccines12080866

Figure Lengend Snippet: Gel image of RVA VP4 and VP7 gene amplicons. Viral protein 4 gene amplicon ( A ): ML is a molecular ladder of 100 plus base pair, L1 to L5 are representative samples with amplified VP4 gene with 877 bp size. Viral protein 7 gene amplicon ( B ). ML is a molecular ladder of 100 plus base pairs, L1, L4, L10, and L12 are lanes with no VP7 gene amplification while the rest of the lanes have the expected VP7 gene amplicon with 1062 base pair size.

Article Snippet: The PCR amplicons derived from VP7 (1062 bp) ( A) and VP4 gene (877 bp) ( B) were extracted from the gel using QIAquick gel extraction kit (Qiagen, Hilden, Germany).

Techniques: Amplification

Journal: Vaccines

Article Title: Rotavirus A Infection Prevalence and Spatio-Temporal Genotype Shift among Under-Five Children in Amhara National Regional State, Ethiopia: A Multi-Center Cross-Sectional Study

doi: 10.3390/vaccines12080866

Figure Lengend Snippet: Phylogenetic analysis of the VP7 gene of RVA strains. Maximum-likelihood trees ( A – E ) were constructed based on the complete VP7 CDS region gene sequences (981 base pairs). A GTR nucleotide substitution model was used to construct the phylogenetic tree. The human and porcine G4 RVA strains were used as the outgroup. Current Ethiopian strains are marked in red dots, previous Ethiopian Strains are marked in blue squares, and RVA vaccine strains are marked in yellow triangles. Bootstrap values (1000 replicates) of ≥50% are shown at each node. The scale represents the rate of nucleotide substitution per site.

Article Snippet: The PCR amplicons derived from VP7 (1062 bp) ( A) and VP4 gene (877 bp) ( B) were extracted from the gel using QIAquick gel extraction kit (Qiagen, Hilden, Germany).

Techniques: Construct

Journal: Vaccines

Article Title: Rotavirus A Infection Prevalence and Spatio-Temporal Genotype Shift among Under-Five Children in Amhara National Regional State, Ethiopia: A Multi-Center Cross-Sectional Study

doi: 10.3390/vaccines12080866

Figure Lengend Snippet: The comparative amino acid sequences analysis of the VP7 antigenic epitopes of the circulating RVA strains (N = 68) with the vaccine strains (Rotarix and RotaTeq).

Article Snippet: The PCR amplicons derived from VP7 (1062 bp) ( A) and VP4 gene (877 bp) ( B) were extracted from the gel using QIAquick gel extraction kit (Qiagen, Hilden, Germany).

Techniques:

Journal: Vaccines

Article Title: Rotavirus A Infection Prevalence and Spatio-Temporal Genotype Shift among Under-Five Children in Amhara National Regional State, Ethiopia: A Multi-Center Cross-Sectional Study

doi: 10.3390/vaccines12080866

Figure Lengend Snippet: Three-dimensional representation of amino acid substitutions detected in VP7 protein of RVA strains (N = 68). Three-dimensional structure of VP7 monomer (light blue color). Antigenic epitopes are colored in green (7-1a), yellow (7-1b), and purple (7-2). Surface-exposed residues that differ between circulating strains in Ethiopia and strains contained in Rotarix or RotaTeq are shown in red color.

Article Snippet: The PCR amplicons derived from VP7 (1062 bp) ( A) and VP4 gene (877 bp) ( B) were extracted from the gel using QIAquick gel extraction kit (Qiagen, Hilden, Germany).

Techniques: