mef  (ATCC)


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    Structured Review

    ATCC mef
    Kinetics and morphology of cell spreading for different cell lines and substrates. (A) Relative area increase for <t>Vero</t> cells in first 20 min after plating. Cells with isotropic spreading (white circles) exhibit more distinct phase transition than anisotropically spread cells (black circles), black triangles represent a graph for whole population. (B) Vero cells spreading morphology on the glass. Scale bar: 10 µm. Cell with isotropic type of spreading extends smooth lamellae on the > 2/3 of circumference, cell with anisotropic spreading extends two or more short lamellae with concave edges. (C) Kinetics of Vero (black triangles), <t>MEF</t> (white circles) and 3T3 (black circles) spreading on glass surface. Vero cells demonstrated most obvious transition between phases, although the type of kinetic curve was similar for all cell lines. (D) Spreading of Vero cells on the glass in presence of serum (white circles), on the poly-L-lysine covered glass in presence of serum (black circles), on the fibronectin-covered glass in presence of serum (black triangles) and on the fibronectin-covered glass in absence of serum (white triangles). In the absence of serum cell spreading on the fibronectin-covered surface is decelerated, but the transition between spreading phases and the type of kinetic curve remains the same. In the presence of both integrin-specific (fibronectin) and nonspecific (vitronectin) ligands spreading kinetics becomes more linear and after 180 min cells continue to spread without transition to polarization.
    Mef, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mef/product/ATCC
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mef - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "Dynamic microtubules drive fibroblast spreading"

    Article Title: Dynamic microtubules drive fibroblast spreading

    Journal: Biology Open

    doi: 10.1242/bio.038968

    Kinetics and morphology of cell spreading for different cell lines and substrates. (A) Relative area increase for Vero cells in first 20 min after plating. Cells with isotropic spreading (white circles) exhibit more distinct phase transition than anisotropically spread cells (black circles), black triangles represent a graph for whole population. (B) Vero cells spreading morphology on the glass. Scale bar: 10 µm. Cell with isotropic type of spreading extends smooth lamellae on the > 2/3 of circumference, cell with anisotropic spreading extends two or more short lamellae with concave edges. (C) Kinetics of Vero (black triangles), MEF (white circles) and 3T3 (black circles) spreading on glass surface. Vero cells demonstrated most obvious transition between phases, although the type of kinetic curve was similar for all cell lines. (D) Spreading of Vero cells on the glass in presence of serum (white circles), on the poly-L-lysine covered glass in presence of serum (black circles), on the fibronectin-covered glass in presence of serum (black triangles) and on the fibronectin-covered glass in absence of serum (white triangles). In the absence of serum cell spreading on the fibronectin-covered surface is decelerated, but the transition between spreading phases and the type of kinetic curve remains the same. In the presence of both integrin-specific (fibronectin) and nonspecific (vitronectin) ligands spreading kinetics becomes more linear and after 180 min cells continue to spread without transition to polarization.
    Figure Legend Snippet: Kinetics and morphology of cell spreading for different cell lines and substrates. (A) Relative area increase for Vero cells in first 20 min after plating. Cells with isotropic spreading (white circles) exhibit more distinct phase transition than anisotropically spread cells (black circles), black triangles represent a graph for whole population. (B) Vero cells spreading morphology on the glass. Scale bar: 10 µm. Cell with isotropic type of spreading extends smooth lamellae on the > 2/3 of circumference, cell with anisotropic spreading extends two or more short lamellae with concave edges. (C) Kinetics of Vero (black triangles), MEF (white circles) and 3T3 (black circles) spreading on glass surface. Vero cells demonstrated most obvious transition between phases, although the type of kinetic curve was similar for all cell lines. (D) Spreading of Vero cells on the glass in presence of serum (white circles), on the poly-L-lysine covered glass in presence of serum (black circles), on the fibronectin-covered glass in presence of serum (black triangles) and on the fibronectin-covered glass in absence of serum (white triangles). In the absence of serum cell spreading on the fibronectin-covered surface is decelerated, but the transition between spreading phases and the type of kinetic curve remains the same. In the presence of both integrin-specific (fibronectin) and nonspecific (vitronectin) ligands spreading kinetics becomes more linear and after 180 min cells continue to spread without transition to polarization.

    Techniques Used: Sublimation

    2) Product Images from "Dynamic microtubules drive fibroblast spreading"

    Article Title: Dynamic microtubules drive fibroblast spreading

    Journal: Biology Open

    doi: 10.1242/bio.038968

    Kinetics and morphology of cell spreading for different cell lines and substrates. (A) Relative area increase for Vero cells in first 20 min after plating. Cells with isotropic spreading (white circles) exhibit more distinct phase transition than anisotropically spread cells (black circles), black triangles represent a graph for whole population. (B) Vero cells spreading morphology on the glass. Scale bar: 10 µm. Cell with isotropic type of spreading extends smooth lamellae on the > 2/3 of circumference, cell with anisotropic spreading extends two or more short lamellae with concave edges. (C) Kinetics of Vero (black triangles), MEF (white circles) and 3T3 (black circles) spreading on glass surface. Vero cells demonstrated most obvious transition between phases, although the type of kinetic curve was similar for all cell lines. (D) Spreading of Vero cells on the glass in presence of serum (white circles), on the poly-L-lysine covered glass in presence of serum (black circles), on the fibronectin-covered glass in presence of serum (black triangles) and on the fibronectin-covered glass in absence of serum (white triangles). In the absence of serum cell spreading on the fibronectin-covered surface is decelerated, but the transition between spreading phases and the type of kinetic curve remains the same. In the presence of both integrin-specific (fibronectin) and nonspecific (vitronectin) ligands spreading kinetics becomes more linear and after 180 min cells continue to spread without transition to polarization.
    Figure Legend Snippet: Kinetics and morphology of cell spreading for different cell lines and substrates. (A) Relative area increase for Vero cells in first 20 min after plating. Cells with isotropic spreading (white circles) exhibit more distinct phase transition than anisotropically spread cells (black circles), black triangles represent a graph for whole population. (B) Vero cells spreading morphology on the glass. Scale bar: 10 µm. Cell with isotropic type of spreading extends smooth lamellae on the > 2/3 of circumference, cell with anisotropic spreading extends two or more short lamellae with concave edges. (C) Kinetics of Vero (black triangles), MEF (white circles) and 3T3 (black circles) spreading on glass surface. Vero cells demonstrated most obvious transition between phases, although the type of kinetic curve was similar for all cell lines. (D) Spreading of Vero cells on the glass in presence of serum (white circles), on the poly-L-lysine covered glass in presence of serum (black circles), on the fibronectin-covered glass in presence of serum (black triangles) and on the fibronectin-covered glass in absence of serum (white triangles). In the absence of serum cell spreading on the fibronectin-covered surface is decelerated, but the transition between spreading phases and the type of kinetic curve remains the same. In the presence of both integrin-specific (fibronectin) and nonspecific (vitronectin) ligands spreading kinetics becomes more linear and after 180 min cells continue to spread without transition to polarization.

    Techniques Used: Sublimation

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    ATCC mouse embryonic fibroblasts mefs
    Mouse Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse embryonic fibroblasts mefs/product/ATCC
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