mouse embryonic fibroblasts  (ATCC)


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    ATCC mouse embryonic fibroblasts
    Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scrc 1040 tm  (ATCC)


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    ATCC scrc 1040 tm
    Scrc 1040 Tm, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts  (ATCC)


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    ATCC mouse embryonic fibroblasts
    Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    embryonic mouse fibroblast cells  (ATCC)


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    ATCC embryonic mouse fibroblast cells
    Embryonic Mouse Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
    STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase <t>nor</t> <t>Src</t> kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. <t>MEF</t> cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The oncogenic FIP1L1-PDGFR α fusion protein displays skewed signaling properties compared to its wild-type PDGFR α counterpart"

    Article Title: The oncogenic FIP1L1-PDGFR α fusion protein displays skewed signaling properties compared to its wild-type PDGFR α counterpart

    Journal: JAK-STAT

    doi: 10.1080/21623996.2015.1062596

    STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase nor Src kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. MEF cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.
    Figure Legend Snippet: STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase nor Src kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. MEF cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.

    Techniques Used: Activity Assay, Activation Assay, Stable Transfection, Expressing, Western Blot, Transfection, Plasmid Preparation, Staining

    embryonic fibroblast feeder cells  (ATCC)


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    ATCC embryonic fibroblast feeder cells
    Embryonic Fibroblast Feeder Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
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    mef cells  (ATCC)


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    ATCC mef cells
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    cf 1 mouse embryonic fibroblasts  (ATCC)


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    ATCC cf 1 mouse embryonic fibroblasts
    J1 mESCs co-cultured with <t>MEFs</t> were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
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    1) Product Images from "Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells"

    Article Title: Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0039329

    J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
    Figure Legend Snippet: J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.

    Techniques Used: Cell Culture, Staining, Activity Assay

    mitomycin c treated mouse embryonic fibroblasts cf 1  (ATCC)


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    ATCC mitomycin c treated mouse embryonic fibroblasts cf 1
    Mitomycin C Treated Mouse Embryonic Fibroblasts Cf 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
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    ATCC mouse embryonic fibroblasts
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    ATCC mef cells
    STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase <t>nor</t> <t>Src</t> kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. <t>MEF</t> cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.
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    ATCC embryonic fibroblast feeder cells
    STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase <t>nor</t> <t>Src</t> kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. <t>MEF</t> cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.
    Embryonic Fibroblast Feeder Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC cf 1 mouse embryonic fibroblasts
    J1 mESCs co-cultured with <t>MEFs</t> were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
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    ATCC mitomycin c treated mouse embryonic fibroblasts cf 1
    J1 mESCs co-cultured with <t>MEFs</t> were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
    Mitomycin C Treated Mouse Embryonic Fibroblasts Cf 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase nor Src kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. MEF cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.

    Journal: JAK-STAT

    Article Title: The oncogenic FIP1L1-PDGFR α fusion protein displays skewed signaling properties compared to its wild-type PDGFR α counterpart

    doi: 10.1080/21623996.2015.1062596

    Figure Lengend Snippet: STAT phosphorylation by F/PDGFRα does neither depend on Janus kinase nor Src kinase activity. ( A ) Janus kinase activity is not required for F/PDGFRα-mediated STAT5 activation. 293FR-cell lines either stably expressing F/PDGFRα or lacking the persistently activated protein (parental cells) were treated with doxycycline (5ng/ml) for 8h. Simultaneously, the pan-JAK inhibitors JAK inhibitor I (JI-I) or INCB-018424/Jakafi® (INCB; 1 μM) were administered to prevent Jak activation. As a control for Janus-kinase mediated STAT-activation, the parental 293FR-cells were pretreated with inhibitors for 8h and additionally stimulated with OSM (25 ng/ml) for 30 min. Activation of pSTAT1, pSTAT3 and pSTAT5 was assessed by Western blot analysis. One representative experiment of at least 3 biological replicates is shown. ( B ) STAT activation by oncogenic PDGFRa proteins does not require Src kinase activity. MEF cells lacking the Src family kinases Yes and Fyn (SRC++ cells) or lacking Src, Yes and Fyn (SYF cells) were transfected with empty vector (mock) or expression plasmids encoding for F/PDGFRα or PDGFRα-D842V. After 48 h, phosphorylation of STAT1, 3 and 5 was monitored by Western blot analysis. A representative tubulin staining is added, showing comparable protein amounts in the samples. One representative experiment of 3 biological replicates is shown.

    Article Snippet: Src++ (ATCC®-CRL-2497™) and SYF (ATCC®-CRL-2459™) MEF cells were purchased from ATCC.

    Techniques: Activity Assay, Activation Assay, Stable Transfection, Expressing, Western Blot, Transfection, Plasmid Preparation, Staining

    J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.

    Journal: PLoS ONE

    Article Title: Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells

    doi: 10.1371/journal.pone.0039329

    Figure Lengend Snippet: J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.

    Article Snippet: CF-1 mouse embryonic fibroblasts (MEF; SCRC-1040, ATCC) were used as a feeder layer for mESC cell culture.

    Techniques: Cell Culture, Staining, Activity Assay