pyoverdine  (ATCC)


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    ATCC pyoverdine
    Bar graphs showing <t>pyoverdine,</t> hemolysin, and swimming motility ( A ), biofilm formation after 24 h incubation at 37 °C ( B ), and pyocyanin and pyorubin production ( C ) by ATCC 10145 and the clinical strain. Error bars indicate the standard deviation. The bars represent the average of three to five replicates per group.
    Pyoverdine, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Proteomic analysis of keratitis-associated Pseudomonas aeruginosa"

    Article Title: Proteomic analysis of keratitis-associated Pseudomonas aeruginosa

    Journal: Molecular Vision

    doi:

    Bar graphs showing pyoverdine, hemolysin, and swimming motility ( A ), biofilm formation after 24 h incubation at 37 °C ( B ), and pyocyanin and pyorubin production ( C ) by ATCC 10145 and the clinical strain. Error bars indicate the standard deviation. The bars represent the average of three to five replicates per group.
    Figure Legend Snippet: Bar graphs showing pyoverdine, hemolysin, and swimming motility ( A ), biofilm formation after 24 h incubation at 37 °C ( B ), and pyocyanin and pyorubin production ( C ) by ATCC 10145 and the clinical strain. Error bars indicate the standard deviation. The bars represent the average of three to five replicates per group.

    Techniques Used: Incubation, Standard Deviation

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    ATCC pseudomonas aeruginosa
    Antibacterial activity of Stachys byzantina , S. officinalis and S. sylvatica extracts on S.A.: Staphylococcus aureus (ATCC 25923), S.E.: Staphylococcus epidermidis (ATCC 12228), B.C.: Bacillus cereus (ATCC 9372), E.F.: Enterococcus faecalis (ATCC 19433), E.C.: Escherichia coli (ATCC 8739), P.A.: Pseudomonas <t>aeruginosa</t> (ATCC 10145), En.C.: Enterobacter cloacae (ATCC 43560), P.M.: Proteus mirabilis (ATCC 29906).
    Pseudomonas Aeruginosa, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Antibacterial activity of Stachys byzantina , S. officinalis and S. sylvatica extracts on S.A.: Staphylococcus aureus (ATCC 25923), S.E.: Staphylococcus epidermidis (ATCC 12228), B.C.: Bacillus cereus (ATCC 9372), E.F.: Enterococcus faecalis (ATCC 19433), E.C.: Escherichia coli (ATCC 8739), P.A.: Pseudomonas aeruginosa (ATCC 10145), En.C.: Enterobacter cloacae (ATCC 43560), P.M.: Proteus mirabilis (ATCC 29906).

    Journal: Plants

    Article Title: Phytochemical Analysis and Biological Activity of Three Stachys Species (Lamiaceae) from Romania

    doi: 10.3390/plants10122710

    Figure Lengend Snippet: Antibacterial activity of Stachys byzantina , S. officinalis and S. sylvatica extracts on S.A.: Staphylococcus aureus (ATCC 25923), S.E.: Staphylococcus epidermidis (ATCC 12228), B.C.: Bacillus cereus (ATCC 9372), E.F.: Enterococcus faecalis (ATCC 19433), E.C.: Escherichia coli (ATCC 8739), P.A.: Pseudomonas aeruginosa (ATCC 10145), En.C.: Enterobacter cloacae (ATCC 43560), P.M.: Proteus mirabilis (ATCC 29906).

    Article Snippet: The bacterial strains used in this study were Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 12228), Bacillus cereus (ATCC 9372), Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 10145), Enterobacter cloacae (ATCC 43560) and Proteus mirabilis (ATCC 29906).

    Techniques: Activity Assay

    Principal component analysis score plots for S. aureus , P. aeruginosa , S. typhi , and K. pneumoniae . λ exc = 380 nm (NADH).

    Journal: International Scholarly Research Notices

    Article Title: Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates

    doi: 10.1155/2014/430412

    Figure Lengend Snippet: Principal component analysis score plots for S. aureus , P. aeruginosa , S. typhi , and K. pneumoniae . λ exc = 380 nm (NADH).

    Article Snippet: Preparation of Bacterial Samples S. aureus (ATCC 6538), P. aeruginosa (ATCC 10145), S. typhi (ATCC 12600), and K. pneumoniae (ATCC 13883) were obtained from the Centre for Advanced Studies in Botany, University of Madras.

    Techniques:

    Tryptophan average steady state emission spectra ( λ exc = 280 nm) for S. aureus , P. aeruginosa , S. typhi , and K. pneumonia.

    Journal: International Scholarly Research Notices

    Article Title: Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates

    doi: 10.1155/2014/430412

    Figure Lengend Snippet: Tryptophan average steady state emission spectra ( λ exc = 280 nm) for S. aureus , P. aeruginosa , S. typhi , and K. pneumonia.

    Article Snippet: Preparation of Bacterial Samples S. aureus (ATCC 6538), P. aeruginosa (ATCC 10145), S. typhi (ATCC 12600), and K. pneumoniae (ATCC 13883) were obtained from the Centre for Advanced Studies in Botany, University of Madras.

    Techniques:

    NADH average steady state emission spectra ( λ exc = 380 nm) for S. aureus , P. aeruginosa , S. typhi , and K. pneumonia.

    Journal: International Scholarly Research Notices

    Article Title: Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates

    doi: 10.1155/2014/430412

    Figure Lengend Snippet: NADH average steady state emission spectra ( λ exc = 380 nm) for S. aureus , P. aeruginosa , S. typhi , and K. pneumonia.

    Article Snippet: Preparation of Bacterial Samples S. aureus (ATCC 6538), P. aeruginosa (ATCC 10145), S. typhi (ATCC 12600), and K. pneumoniae (ATCC 13883) were obtained from the Centre for Advanced Studies in Botany, University of Madras.

    Techniques:

    Pyocyanin production in P. aeruginosa bacteria (1.5 × 10 5 CFU/well) after 36 hours of exposure to various concentrations of royal jelly. Pyocyanin production increased due to exposure to sub-inhibitory royal jelly concentrations of 6.25% and decreased at lower royal jelly concentrations. The difference was based on the results of the Tukey-HSD analysis at the significance value (*) p

    Journal: F1000Research

    Article Title: Enhancement of pyocyanin production by subinhibitory concentration of royal jelly inPseudomonas aeruginosa

    doi: 10.12688/f1000research.27915.2

    Figure Lengend Snippet: Pyocyanin production in P. aeruginosa bacteria (1.5 × 10 5 CFU/well) after 36 hours of exposure to various concentrations of royal jelly. Pyocyanin production increased due to exposure to sub-inhibitory royal jelly concentrations of 6.25% and decreased at lower royal jelly concentrations. The difference was based on the results of the Tukey-HSD analysis at the significance value (*) p

    Article Snippet: One-way ANOVA showed a significant difference in the percentage of growth inhibition inP. aeruginosa ATCC® 10145™ (p = 0.000) andP. aeruginosa clinical isolate (p = 0.000) between royal jelly treatment groups and negative control.

    Techniques:

    The 12.5% royal jelly increased the formation of P. aeruginosa biofilm stronger than 25% and 6.25% royal jelly. ATCC 10145.

    Journal: F1000Research

    Article Title: Enhancement of pyocyanin production by subinhibitory concentration of royal jelly inPseudomonas aeruginosa

    doi: 10.12688/f1000research.27915.2

    Figure Lengend Snippet: The 12.5% royal jelly increased the formation of P. aeruginosa biofilm stronger than 25% and 6.25% royal jelly. ATCC 10145.

    Article Snippet: One-way ANOVA showed a significant difference in the percentage of growth inhibition inP. aeruginosa ATCC® 10145™ (p = 0.000) andP. aeruginosa clinical isolate (p = 0.000) between royal jelly treatment groups and negative control.

    Techniques:

    Percentage of inhibition of growth of P. aeruginosa bacteria (1.5 × 10 5 CFU/well). Bacterial cultures were incubated with varying concentrations of royal jelly for 18 hours at 37°C. Royal jelly 50% and 25% inhibit bacterial growth. The difference is based on the results of the Games-Howell analysis at the significance value (*) p

    Journal: F1000Research

    Article Title: Enhancement of pyocyanin production by subinhibitory concentration of royal jelly inPseudomonas aeruginosa

    doi: 10.12688/f1000research.27915.2

    Figure Lengend Snippet: Percentage of inhibition of growth of P. aeruginosa bacteria (1.5 × 10 5 CFU/well). Bacterial cultures were incubated with varying concentrations of royal jelly for 18 hours at 37°C. Royal jelly 50% and 25% inhibit bacterial growth. The difference is based on the results of the Games-Howell analysis at the significance value (*) p

    Article Snippet: One-way ANOVA showed a significant difference in the percentage of growth inhibition inP. aeruginosa ATCC® 10145™ (p = 0.000) andP. aeruginosa clinical isolate (p = 0.000) between royal jelly treatment groups and negative control.

    Techniques: Inhibition, Incubation

    Pyocyanin was identified from the greenish colour of the P. aeruginosa culture supernatant after 36 h incubation at 37°C. P. aeruginosa bacteria (1.5 × 10 5 CFU/well) ATCC 10145 ( a ); clinical isolate ( b ). Royal jelly 12.5% (A); 6.25% (B); 3,125% (C); 1.56% (D); 0.78% (E); 0.39% (F); 0.19% (G); 0.098% (H); 0% (I); No treatment (J).

    Journal: F1000Research

    Article Title: Enhancement of pyocyanin production by subinhibitory concentration of royal jelly inPseudomonas aeruginosa

    doi: 10.12688/f1000research.27915.2

    Figure Lengend Snippet: Pyocyanin was identified from the greenish colour of the P. aeruginosa culture supernatant after 36 h incubation at 37°C. P. aeruginosa bacteria (1.5 × 10 5 CFU/well) ATCC 10145 ( a ); clinical isolate ( b ). Royal jelly 12.5% (A); 6.25% (B); 3,125% (C); 1.56% (D); 0.78% (E); 0.39% (F); 0.19% (G); 0.098% (H); 0% (I); No treatment (J).

    Article Snippet: One-way ANOVA showed a significant difference in the percentage of growth inhibition inP. aeruginosa ATCC® 10145™ (p = 0.000) andP. aeruginosa clinical isolate (p = 0.000) between royal jelly treatment groups and negative control.

    Techniques: Incubation

    Scanning electron micrograph of P. aeruginosa biofilm showed the formation of specific bacterial colonies on the adhesion surface with extensions indicating bacterial motility in the group exposed to royal jelly 6.25% ( A ), complete coverage of the adhesion surface by the bacterial biofilm mass in the treatment group with royal jelly 12.5% ( B ). Adhesion surface was free from bacterial biofilm in the bacterial group treated with 25% royal jelly ( C ). Figure D shows the biofilm architecture of the 6.25% royal jelly group, and E is that of the 12.5% royal jelly group at a magnification of x5000. The negative control group showed evenly distributed bacterial colonies on the adhesion surface ( F ), while the positive control group (chlorhexidine) showed complete inhibition of biofilm formation ( G ). Overall, the SEM results presented the highest increase in biofilm mass formation by 12.5% royal jelly. ATCC 10145 strain

    Journal: F1000Research

    Article Title: Enhancement of pyocyanin production by subinhibitory concentration of royal jelly inPseudomonas aeruginosa

    doi: 10.12688/f1000research.27915.2

    Figure Lengend Snippet: Scanning electron micrograph of P. aeruginosa biofilm showed the formation of specific bacterial colonies on the adhesion surface with extensions indicating bacterial motility in the group exposed to royal jelly 6.25% ( A ), complete coverage of the adhesion surface by the bacterial biofilm mass in the treatment group with royal jelly 12.5% ( B ). Adhesion surface was free from bacterial biofilm in the bacterial group treated with 25% royal jelly ( C ). Figure D shows the biofilm architecture of the 6.25% royal jelly group, and E is that of the 12.5% royal jelly group at a magnification of x5000. The negative control group showed evenly distributed bacterial colonies on the adhesion surface ( F ), while the positive control group (chlorhexidine) showed complete inhibition of biofilm formation ( G ). Overall, the SEM results presented the highest increase in biofilm mass formation by 12.5% royal jelly. ATCC 10145 strain

    Article Snippet: One-way ANOVA showed a significant difference in the percentage of growth inhibition inP. aeruginosa ATCC® 10145™ (p = 0.000) andP. aeruginosa clinical isolate (p = 0.000) between royal jelly treatment groups and negative control.

    Techniques: Negative Control, Positive Control, Inhibition

    Membrane damage in P. aeruginosa ATCC 10145 and S. thermophilus DSM 20617 T exposed to surfactin. Flow cytometry density diagrams show the SYBR Green I vs PI fluorescence of cells exposed to promysalin or chlorhexidine or both the molecules. Viable cells are gated in G1, viable cells with slightly damaged cell membrane are gated in G2. Dead cells with damaged membrane are gated in G3. The transition of cell population from gate G1 to gate G3 is related to the entity of cell membrane damage. ( a ) S. thermophilus incubated 60 min at 37 °C in presence of DMSO. ( b ) S. thermophilus incubated 60 min at 37 °C in presence of surfactin (200 µg/ml). ( C ) P. aeruginosa cells incubated 60 min at 37 °C in presence of DMSO. ( d ) P. aeruginosa cells incubated 60 min at 37 °C in presence of surfactin (200 µg/ml). DMSO was added to cell suspension in the same amount which was present in the surfactin solution.

    Journal: Scientific Reports

    Article Title: Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria

    doi: 10.1038/s41598-017-07567-0

    Figure Lengend Snippet: Membrane damage in P. aeruginosa ATCC 10145 and S. thermophilus DSM 20617 T exposed to surfactin. Flow cytometry density diagrams show the SYBR Green I vs PI fluorescence of cells exposed to promysalin or chlorhexidine or both the molecules. Viable cells are gated in G1, viable cells with slightly damaged cell membrane are gated in G2. Dead cells with damaged membrane are gated in G3. The transition of cell population from gate G1 to gate G3 is related to the entity of cell membrane damage. ( a ) S. thermophilus incubated 60 min at 37 °C in presence of DMSO. ( b ) S. thermophilus incubated 60 min at 37 °C in presence of surfactin (200 µg/ml). ( C ) P. aeruginosa cells incubated 60 min at 37 °C in presence of DMSO. ( d ) P. aeruginosa cells incubated 60 min at 37 °C in presence of surfactin (200 µg/ml). DMSO was added to cell suspension in the same amount which was present in the surfactin solution.

    Article Snippet: Unfortunately, when promysalin was tested against the sensitive Pseudomonas stutzeri LMG 2333 and P. aeruginosa ATCC 10145 by flow cytometry, a moderate or no cell membrane damage was observed, even if the exposition of bacterial cells to promysalin was prolonged for several hours at 37 °C (Figures – ).

    Techniques: Flow Cytometry, Cytometry, SYBR Green Assay, Fluorescence, Incubation

    Transmission Electron Microscope images of P. aeruginosa ATCC 10145 and S. thermophilus DSM 20617 T before and after exposure to chlorhexidine and promysalin. ( a ) P. aeruginosa cell not exposed and ( b ) exposed to chlorhexidine (100 µg/ml) or ( c ) to promysalin (100 µg/ml). ( d ) S. thermophilus cell not exposed and ( e ) exposed to chlorhexidine (100 µg/ml) or ( f ) to promysalin (100 µg/ml). Black arrows indicate the membrane protrusions in cell exposed to chlorhexidine.

    Journal: Scientific Reports

    Article Title: Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria

    doi: 10.1038/s41598-017-07567-0

    Figure Lengend Snippet: Transmission Electron Microscope images of P. aeruginosa ATCC 10145 and S. thermophilus DSM 20617 T before and after exposure to chlorhexidine and promysalin. ( a ) P. aeruginosa cell not exposed and ( b ) exposed to chlorhexidine (100 µg/ml) or ( c ) to promysalin (100 µg/ml). ( d ) S. thermophilus cell not exposed and ( e ) exposed to chlorhexidine (100 µg/ml) or ( f ) to promysalin (100 µg/ml). Black arrows indicate the membrane protrusions in cell exposed to chlorhexidine.

    Article Snippet: Unfortunately, when promysalin was tested against the sensitive Pseudomonas stutzeri LMG 2333 and P. aeruginosa ATCC 10145 by flow cytometry, a moderate or no cell membrane damage was observed, even if the exposition of bacterial cells to promysalin was prolonged for several hours at 37 °C (Figures – ).

    Techniques: Transmission Assay, Microscopy

    Membrane damage in P. aeruginosa ATCC 10145 exposed to promysalin, chlorhexidine, and to a mixture of the two molecules. Flow cytometry density diagrams show the SYBR Green I vs PI fluorescence of cells exposed to promysalin or chlorhexidine or both the molecules. Viable cells are gated in G1, viable cells with slightly damaged cell membrane are gated in G2. Dead cells with damaged membrane are gated in G3. The transition of cell population from gate G1 to gate G3 is related to the entity of cell membrane damage. ( a ) P. aeruginosa unlabeled cells. ( b ) P. aeruginosa cells incubated 60 min at 37 °C. ( c ) P. aeruginosa cells incubated 60 min at 37 °C in presence of DMSO. ( d ) P. aeruginosa cells incubated 60 min at 37 °C in presence of chlorhexidine (10 µg/ml). ( e ) P. aeruginosa cells incubated 60 min at 37 °C in presence of promysalin (100 µg/ml). ( F ) P. aeruginosa cells incubated 60 min at 37 °C in presence of chlorhexidine and promysalin (10 µg/ml and 100 µg/ml respectively). DMSO was added to cell suspension in the same amount which was present in the promysalin solution.

    Journal: Scientific Reports

    Article Title: Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria

    doi: 10.1038/s41598-017-07567-0

    Figure Lengend Snippet: Membrane damage in P. aeruginosa ATCC 10145 exposed to promysalin, chlorhexidine, and to a mixture of the two molecules. Flow cytometry density diagrams show the SYBR Green I vs PI fluorescence of cells exposed to promysalin or chlorhexidine or both the molecules. Viable cells are gated in G1, viable cells with slightly damaged cell membrane are gated in G2. Dead cells with damaged membrane are gated in G3. The transition of cell population from gate G1 to gate G3 is related to the entity of cell membrane damage. ( a ) P. aeruginosa unlabeled cells. ( b ) P. aeruginosa cells incubated 60 min at 37 °C. ( c ) P. aeruginosa cells incubated 60 min at 37 °C in presence of DMSO. ( d ) P. aeruginosa cells incubated 60 min at 37 °C in presence of chlorhexidine (10 µg/ml). ( e ) P. aeruginosa cells incubated 60 min at 37 °C in presence of promysalin (100 µg/ml). ( F ) P. aeruginosa cells incubated 60 min at 37 °C in presence of chlorhexidine and promysalin (10 µg/ml and 100 µg/ml respectively). DMSO was added to cell suspension in the same amount which was present in the promysalin solution.

    Article Snippet: Unfortunately, when promysalin was tested against the sensitive Pseudomonas stutzeri LMG 2333 and P. aeruginosa ATCC 10145 by flow cytometry, a moderate or no cell membrane damage was observed, even if the exposition of bacterial cells to promysalin was prolonged for several hours at 37 °C (Figures – ).

    Techniques: Flow Cytometry, Cytometry, SYBR Green Assay, Fluorescence, Incubation