Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Vaginal smear of Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups showing A (proestrus), B (estrus), C (metestrus) and D (diestrus). Nucleated epithelia cells (white arrow), cornified epithelia cells (red arrow) and leukocytes (yellow arrow) were observed (crystal violet stain, X100).

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Negative Control, Positive Control, Staining

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Percentage of mice that restored normal estrus cycle among Negative control (NC), MitoQ 10 (MQ), Vitamin D3 (VD), Vitamin D3 vehicle (VDV), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Negative Control, Positive Control

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Histomorphological sections of ovaries from : Negative control (NC): A&B , Vitamin D3 vehicle (VDV): C&D, MitoQ 10 (MQ): E&F , Vitamin D3 (VD): G&H , Vitamin D3 plus MitoQ 10 (V+M): I&J and Positive control (PC): K&L groups. In A, B, C and D sections, many follicular cyst ( FC ) and atretic follicle ( AtF ) but no corpus luteum ( CL ) were observed. E, F, G, H, I, J, K and L sections show various stages of follicular development such as pre-antral follicle, antral follicle ( AF ), few atretic follicle ( AtF ) and many corpora lutea ( CL ) (H&E, A C E G I K: × 40, B D F H J L : × 100).

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Negative Control, Positive Control

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Relative expression of mRNAs of A : 3β-hydroxysteroid dehydrogenase ( 3β-HSD ), B : steroidogenic acute regulatory ( StAR ), C : cytochrome P450 family 11 subfamily member 1 ( Cyp11a1 ), D : cytochrome P450 aromatase ( Cyp19a1 ) by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups. # indicate p > 0.05, ∗ indicates p < 0.05 and ∗∗ indicates p < 0.01 when the rate of expression of the other groups was compared with that of NC group.

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Expressing, Negative Control, Positive Control

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups. # indicate p > 0.05 ∗ indicates p < 0.05 and ∗∗ indicates p < 0.01 when the mean level of the other groups was compared with that of NC group. (Full-unadjusted images are shown in supplementary material 1, 2 and 5).

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Expressing, Negative Control, Positive Control

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Relative expression of mRNAs of A : Heme oxygenase-1 ( HO-1 ) , B : Nuclear factor erythroid-2-related factor 2 ( Nrf2 ) and C : Kelch-like ECH-associated protein 1 ( Keap1 ) by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups. # indicate p > 0.05, ∗ indicates p < 0.05 and ∗∗ indicates p < 0.01 when the rate of expression of the other groups was compared with that of NC group.

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Expressing, Negative Control, Positive Control

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Expression of A: Heme oxygenase-1 ( HO-1 ) and B: Nuclear factor erythroid-2-related factor 2 ( Nrf2 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups. # indicate p > 0.05 ∗ indicates p < 0.05 and ∗∗ indicates p < 0.01 when the mean level of the other groups was compared with that of NC group. (Full-unadjusted images are shown in supplementary material 3, 4 and 5).

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Expressing, Negative Control, Positive Control

Journal: Heliyon

Article Title: Assessing the effect of MitoQ 10 and Vitamin D3 on ovarian oxidative stress, steroidogenesis and histomorphology in DHEA induced PCOS mouse model

doi: 10.1016/j.heliyon.2020.e04279

Figure Lengend Snippet: Amount of A : serum Malondialldehyde (MDA), B : serum superoxide dismutase (SOD) activities and C : ovarian tissue superoxide dismutase (SOD) activities of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V + M) and Positive Control (PC) groups. # indicate p > 0.05 ∗ indicates p < 0.05 and ∗∗ indicates p < 0.01 when the mean level of the other groups was compared with that of NC group.

Article Snippet: Expression of A: 3β-hydroxysteroid dehydrogenase ( 3β-HSD ) and B: cytochrome P450 aromatase ( Cyp19a1 ) proteins by ovaries of mice in Negative control (NC), Vitamin D3 vehicle (VDV), Vitamin D3 (VD), MitoQ 10 (MQ), Vitamin D3 plus MitoQ 10 (V+M) and Positive Control (PC) groups.

Techniques: Negative Control, Positive Control