kb dna ladder  (Solis BioDyne)


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    Structured Review

    Solis BioDyne kb dna ladder
    Electrophoretic analysis of genomic <t>DNA</t> from biobanked frozen tumor samples. DNA (5 μL) was loaded on a 2% agarose gel and visualized by ethidium bromide staining. The gel shows the result of 10 representative samples. Compact bands of DNA were observed for all samples at a high molecular weight according to the <t>ladder.</t> The absence of smearing favors the absence of DNA degradation.
    Kb Dna Ladder, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kb dna ladder/product/Solis BioDyne
    Average 93 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    kb dna ladder - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Tumor Banks: A Quality Control Scheme Proposal"

    Article Title: Tumor Banks: A Quality Control Scheme Proposal

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2019.00225

    Electrophoretic analysis of genomic DNA from biobanked frozen tumor samples. DNA (5 μL) was loaded on a 2% agarose gel and visualized by ethidium bromide staining. The gel shows the result of 10 representative samples. Compact bands of DNA were observed for all samples at a high molecular weight according to the ladder. The absence of smearing favors the absence of DNA degradation.
    Figure Legend Snippet: Electrophoretic analysis of genomic DNA from biobanked frozen tumor samples. DNA (5 μL) was loaded on a 2% agarose gel and visualized by ethidium bromide staining. The gel shows the result of 10 representative samples. Compact bands of DNA were observed for all samples at a high molecular weight according to the ladder. The absence of smearing favors the absence of DNA degradation.

    Techniques Used: Agarose Gel Electrophoresis, Staining, Molecular Weight

    2) Product Images from "A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study"

    Article Title: A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

    Journal: Molecules

    doi: 10.3390/molecules24234335

    Gel electrophoregram of the interaction of phenolic compounds (PhC) and their Cu(II) complexes with plasmid DNA in the absence of hydrogen peroxide (non-Fenton system). Electrophoretic profile of agarose gel (0.8%) of the reaction mixture of 20 µM pBSK+ DNA, free phenolic compounds (lanes 1–5) and Cu(II) complexes with phenolic compounds (lanes 6–10), incubated at RT for 30 min. The order of the columns in the gel: (p) control pDNA; (1–5) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM), (6–10) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM) + 5 µM Cu(II). Experimental conditions of all reactions were the same, so the observed differences in gels are related to structural differences of phenolic compounds. Due to the different solubility of phenolic compounds, individual phenolic compounds were used under different concentrations. (PhC = Phenolic compound; MYR = Myricetin; MOR = Morin; DHF = 3′,4′-dihydroxyflavone; TAX = Taxifolin; CUM = 4-hydroxycoumarin).
    Figure Legend Snippet: Gel electrophoregram of the interaction of phenolic compounds (PhC) and their Cu(II) complexes with plasmid DNA in the absence of hydrogen peroxide (non-Fenton system). Electrophoretic profile of agarose gel (0.8%) of the reaction mixture of 20 µM pBSK+ DNA, free phenolic compounds (lanes 1–5) and Cu(II) complexes with phenolic compounds (lanes 6–10), incubated at RT for 30 min. The order of the columns in the gel: (p) control pDNA; (1–5) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM), (6–10) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM) + 5 µM Cu(II). Experimental conditions of all reactions were the same, so the observed differences in gels are related to structural differences of phenolic compounds. Due to the different solubility of phenolic compounds, individual phenolic compounds were used under different concentrations. (PhC = Phenolic compound; MYR = Myricetin; MOR = Morin; DHF = 3′,4′-dihydroxyflavone; TAX = Taxifolin; CUM = 4-hydroxycoumarin).

    Techniques Used: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation, Solubility

    Absorption spectra obtained by titration of free phenolic compound (20 μM) and phenolic compound in the presence of Cu(II) (Cu:phenolic compound = 1:2) with CT-DNA (0–50 μM) in a solution containing 5 mM Tris-HCl, 50 mM NaCl, pH 7.2 in the absence of CT-DNA (red line) and in the presence of an increasing concentration of CT-DNA (0–50 μM). The direction of the arrow indicates the shift in the absorbance after addition of the CT-DNA solution. Abbreviations: myricetin (MYR), morin (MOR), 3′,4′-dihydroxyflavone (DHF), taxifolin (TAX), 4-hydroxycoumarin (CUM).
    Figure Legend Snippet: Absorption spectra obtained by titration of free phenolic compound (20 μM) and phenolic compound in the presence of Cu(II) (Cu:phenolic compound = 1:2) with CT-DNA (0–50 μM) in a solution containing 5 mM Tris-HCl, 50 mM NaCl, pH 7.2 in the absence of CT-DNA (red line) and in the presence of an increasing concentration of CT-DNA (0–50 μM). The direction of the arrow indicates the shift in the absorbance after addition of the CT-DNA solution. Abbreviations: myricetin (MYR), morin (MOR), 3′,4′-dihydroxyflavone (DHF), taxifolin (TAX), 4-hydroxycoumarin (CUM).

    Techniques Used: Titration, Concentration Assay

    Gel electrophoregram of the interaction of phenolic compounds (PhC) and plasmid DNA in the presence of hydrogen peroxide (Fenton system). Electrophoretic profile (0.8% agarose gel) of DNA protection against oxidative damage in the reaction mixture (20 µL) containing Cu(II) ions, phenolic compounds, pBSK+ DNA, hydrogen peroxide and incubated at RT for 30 min. The order of the samples in the gel: (p) control pDNA; (C) Fenton like reaction containing pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 ; (1–8) Fenton like reaction + phenolic compound at concentrations of 5–500 µM (MYR, MOR, DHF) or 5–5000 µM (TAX, CUM); (L) 1 kb DNA standard (LIN) linearized pDNA.
    Figure Legend Snippet: Gel electrophoregram of the interaction of phenolic compounds (PhC) and plasmid DNA in the presence of hydrogen peroxide (Fenton system). Electrophoretic profile (0.8% agarose gel) of DNA protection against oxidative damage in the reaction mixture (20 µL) containing Cu(II) ions, phenolic compounds, pBSK+ DNA, hydrogen peroxide and incubated at RT for 30 min. The order of the samples in the gel: (p) control pDNA; (C) Fenton like reaction containing pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 ; (1–8) Fenton like reaction + phenolic compound at concentrations of 5–500 µM (MYR, MOR, DHF) or 5–5000 µM (TAX, CUM); (L) 1 kb DNA standard (LIN) linearized pDNA.

    Techniques Used: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation

    Relative densitometric quantification of band intensities of, at least, 3 agarose gels as illustrated in Figure 9 . Green colour corresponds to the SC form of plasmid DNA, red colour to the LIN form and orange colour to the OC form. Taxifolin and coumarin were tested up to a concentration of 5000 μM. p—SC form of pDNA, C —proportion of pDNA conformations formed in Fenton like reaction, 1–8—proportion of pDNA conformations formed in Fenton like reaction in the presence of phenolic compound. Standard deviations are expressed as error bars.
    Figure Legend Snippet: Relative densitometric quantification of band intensities of, at least, 3 agarose gels as illustrated in Figure 9 . Green colour corresponds to the SC form of plasmid DNA, red colour to the LIN form and orange colour to the OC form. Taxifolin and coumarin were tested up to a concentration of 5000 μM. p—SC form of pDNA, C —proportion of pDNA conformations formed in Fenton like reaction, 1–8—proportion of pDNA conformations formed in Fenton like reaction in the presence of phenolic compound. Standard deviations are expressed as error bars.

    Techniques Used: Plasmid Preparation, Concentration Assay

    3) Product Images from "Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study"

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22041619

    Electrophoretic profile of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu (II) ions and hydrogen peroxide. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; (1) pDNA + 5 µM CuCl 2 , 50 µM H 2 O 2 , (2–4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 plus (2) L-histidine, (3) DMSO, (4) SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.
    Figure Legend Snippet: Electrophoretic profile of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu (II) ions and hydrogen peroxide. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; (1) pDNA + 5 µM CuCl 2 , 50 µM H 2 O 2 , (2–4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 plus (2) L-histidine, (3) DMSO, (4) SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol, (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol; (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm −3 ).
    Figure Legend Snippet: Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol, (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol; (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm −3 ).

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    Electrophoretic profile of agarose gel (0.8%), 15 µM plasmid PBSK + DNA with Cu(II) ions, hydrogen peroxide and kaempferol incubated for 30 min. Samples were loaded in the gel lanes in the following order: (p) pDNA–control; (K) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 -control Cu-Fenton reaction; (1–8) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + (1) 5 µM kaempferol, (2) 10 µM kaempferol, (3) 50 µM kaempferol, (4) 100 µM kaempferol, (5) 200 µM kaempferol, (6) 300 µM kaempferol, (7) 400 µM kaempferol, (8) 500 µM kaempferol; (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.
    Figure Legend Snippet: Electrophoretic profile of agarose gel (0.8%), 15 µM plasmid PBSK + DNA with Cu(II) ions, hydrogen peroxide and kaempferol incubated for 30 min. Samples were loaded in the gel lanes in the following order: (p) pDNA–control; (K) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 -control Cu-Fenton reaction; (1–8) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + (1) 5 µM kaempferol, (2) 10 µM kaempferol, (3) 50 µM kaempferol, (4) 100 µM kaempferol, (5) 200 µM kaempferol, (6) 300 µM kaempferol, (7) 400 µM kaempferol, (8) 500 µM kaempferol; (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation, Incubation

    Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).
    Figure Legend Snippet: Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).

    Techniques Used: Concentration Assay

    4) Product Images from "NGS study of glucocorticoid response genes in inflammatory bowel disease patients"

    Article Title: NGS study of glucocorticoid response genes in inflammatory bowel disease patients

    Journal: Archives of Medical Science : AMS

    doi: 10.5114/aoms.2019.84470

    Agarose electrophoresis of all 54 LR-PCR products for one DNA sample. Lane: 1–5 NR3C1 (2400 bp, 6301 bp, 5031 bp, 6208 bp, 2261 bp), 6–10 – NLRP1 (5287 bp, 7575 bp, 12504 bp, 5023 bp, 1088 bp), 11–13 – IPO13 (4357 bp, 6294 bp, 4762 bp), 14–19 – FKBP5 (2935 bp, 2534 bp, 6516 bp, 2523 bp, 7518 bp, 12540 bp), 20–23 – HSPA4 (1290 bp, 13489 bp, 7051 bp, 9368 bp), 24–29 – ABCB1 (3518 bp, 15887 bp, 16809 bp, 14483 bp, 17000 bp, 5967 bp), 30–32 – STIP1 (2224 bp, 6456 bp, 5400 bp), 33–35 – HSP90AA1 (1404 bp, 1238 bp, 5157 bp), 36 – IL1A (12577 bp), 37 – IL1B (10196 bp), 38 – IL2 (6505 bp), 39 – IL4 (10003 bp), 40 – CXCL8 (5000 bp), 41 – IL10 (6199 bp), 42 – NFKBI (5016 bp), 43 – JUN (4730 bp), 44 – MIF (2731 bp), 45 – TNF (8831 bp), 46–49 – MAPK14 (1910 bp, 7515 bp, 4900 bp, 13300 bp), 50–51 – CYP3A4 (10626 bp, 15518 bp), 52–54 – CYP3A5 (8829 bp, 7268 bp, 4910 bp), 1 kb – marker ladder 1000 bp. The gel images were obtained by trimming and color adjusting of the full-length gels in the IrfanView 4.44 program. The lanes numbering corresponds with the numbering of the amplified fragments in Tables III and IV
    Figure Legend Snippet: Agarose electrophoresis of all 54 LR-PCR products for one DNA sample. Lane: 1–5 NR3C1 (2400 bp, 6301 bp, 5031 bp, 6208 bp, 2261 bp), 6–10 – NLRP1 (5287 bp, 7575 bp, 12504 bp, 5023 bp, 1088 bp), 11–13 – IPO13 (4357 bp, 6294 bp, 4762 bp), 14–19 – FKBP5 (2935 bp, 2534 bp, 6516 bp, 2523 bp, 7518 bp, 12540 bp), 20–23 – HSPA4 (1290 bp, 13489 bp, 7051 bp, 9368 bp), 24–29 – ABCB1 (3518 bp, 15887 bp, 16809 bp, 14483 bp, 17000 bp, 5967 bp), 30–32 – STIP1 (2224 bp, 6456 bp, 5400 bp), 33–35 – HSP90AA1 (1404 bp, 1238 bp, 5157 bp), 36 – IL1A (12577 bp), 37 – IL1B (10196 bp), 38 – IL2 (6505 bp), 39 – IL4 (10003 bp), 40 – CXCL8 (5000 bp), 41 – IL10 (6199 bp), 42 – NFKBI (5016 bp), 43 – JUN (4730 bp), 44 – MIF (2731 bp), 45 – TNF (8831 bp), 46–49 – MAPK14 (1910 bp, 7515 bp, 4900 bp, 13300 bp), 50–51 – CYP3A4 (10626 bp, 15518 bp), 52–54 – CYP3A5 (8829 bp, 7268 bp, 4910 bp), 1 kb – marker ladder 1000 bp. The gel images were obtained by trimming and color adjusting of the full-length gels in the IrfanView 4.44 program. The lanes numbering corresponds with the numbering of the amplified fragments in Tables III and IV

    Techniques Used: Electrophoresis, Polymerase Chain Reaction, Marker, Amplification

    5) Product Images from "Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study"

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22041619

    Electrophoretic profile of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu (II) ions and hydrogen peroxide. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; (1) pDNA + 5 µM CuCl 2 , 50 µM H 2 O 2 , (2–4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 plus (2) L-histidine, (3) DMSO, (4) SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.
    Figure Legend Snippet: Electrophoretic profile of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu (II) ions and hydrogen peroxide. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; (1) pDNA + 5 µM CuCl 2 , 50 µM H 2 O 2 , (2–4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 plus (2) L-histidine, (3) DMSO, (4) SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol, (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol; (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm −3 ).
    Figure Legend Snippet: Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol, (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol; (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm −3 ).

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    Electrophoretic profile of agarose gel (0.8%), 15 µM plasmid PBSK + DNA with Cu(II) ions, hydrogen peroxide and kaempferol incubated for 30 min. Samples were loaded in the gel lanes in the following order: (p) pDNA–control; (K) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 -control Cu-Fenton reaction; (1–8) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + (1) 5 µM kaempferol, (2) 10 µM kaempferol, (3) 50 µM kaempferol, (4) 100 µM kaempferol, (5) 200 µM kaempferol, (6) 300 µM kaempferol, (7) 400 µM kaempferol, (8) 500 µM kaempferol; (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.
    Figure Legend Snippet: Electrophoretic profile of agarose gel (0.8%), 15 µM plasmid PBSK + DNA with Cu(II) ions, hydrogen peroxide and kaempferol incubated for 30 min. Samples were loaded in the gel lanes in the following order: (p) pDNA–control; (K) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 -control Cu-Fenton reaction; (1–8) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + (1) 5 µM kaempferol, (2) 10 µM kaempferol, (3) 50 µM kaempferol, (4) 100 µM kaempferol, (5) 200 µM kaempferol, (6) 300 µM kaempferol, (7) 400 µM kaempferol, (8) 500 µM kaempferol; (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation, Incubation

    Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).
    Figure Legend Snippet: Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).

    Techniques Used: Concentration Assay

    6) Product Images from "Synthesis, Crystal Structure, Spectroscopic Properties and Potential Biological Activities of Salicylate‒Neocuproine Ternary Copper(II) Complexes"

    Article Title: Synthesis, Crystal Structure, Spectroscopic Properties and Potential Biological Activities of Salicylate‒Neocuproine Ternary Copper(II) Complexes

    Journal: Molecules

    doi: 10.3390/molecules20022115

    Agarose gel electrophoresis (0.8%) of pBSK+ plasmid cleavage by Cu(II) complexes. All reactions were in a final volume of 20 μL, for 1 h at 37 °C. Lanes represent: (p) control plasmid, (1–5) plasmid + copper complex (5, 10, 25, 40, 50 µM); (6–10) plasmid + copper complex (5, 10, 25, 40, 50 µM) each with 50 µM H 2 O 2 ; (L) 1kb DNA ladder, (lin) plasmid linearised with Eco RI restrictase. ( A – C ) refer to complex 1 , 2 and 3 , respectively.
    Figure Legend Snippet: Agarose gel electrophoresis (0.8%) of pBSK+ plasmid cleavage by Cu(II) complexes. All reactions were in a final volume of 20 μL, for 1 h at 37 °C. Lanes represent: (p) control plasmid, (1–5) plasmid + copper complex (5, 10, 25, 40, 50 µM); (6–10) plasmid + copper complex (5, 10, 25, 40, 50 µM) each with 50 µM H 2 O 2 ; (L) 1kb DNA ladder, (lin) plasmid linearised with Eco RI restrictase. ( A – C ) refer to complex 1 , 2 and 3 , respectively.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    Agarose gel electrophoresis (0.8%) of pBSK+. Lanes represent: (p) control plasmid, (1) plasmid + H 2 O 2 , (2) plasmid + DMSO, (L) 1kb DNA ladder, (lin) plasmid linearised with Eco RI restrictase.
    Figure Legend Snippet: Agarose gel electrophoresis (0.8%) of pBSK+. Lanes represent: (p) control plasmid, (1) plasmid + H 2 O 2 , (2) plasmid + DMSO, (L) 1kb DNA ladder, (lin) plasmid linearised with Eco RI restrictase.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    7) Product Images from "A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study"

    Article Title: A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

    Journal: Molecules

    doi: 10.3390/molecules24234335

    Gel electrophoregram of the interaction of phenolic compounds (PhC) and their Cu(II) complexes with plasmid DNA in the absence of hydrogen peroxide (non-Fenton system). Electrophoretic profile of agarose gel (0.8%) of the reaction mixture of 20 µM pBSK+ DNA, free phenolic compounds (lanes 1–5) and Cu(II) complexes with phenolic compounds (lanes 6–10), incubated at RT for 30 min. The order of the columns in the gel: (p) control pDNA; (1–5) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM), (6–10) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM) + 5 µM Cu(II). Experimental conditions of all reactions were the same, so the observed differences in gels are related to structural differences of phenolic compounds. Due to the different solubility of phenolic compounds, individual phenolic compounds were used under different concentrations. (PhC = Phenolic compound; MYR = Myricetin; MOR = Morin; DHF = 3′,4′-dihydroxyflavone; TAX = Taxifolin; CUM = 4-hydroxycoumarin).
    Figure Legend Snippet: Gel electrophoregram of the interaction of phenolic compounds (PhC) and their Cu(II) complexes with plasmid DNA in the absence of hydrogen peroxide (non-Fenton system). Electrophoretic profile of agarose gel (0.8%) of the reaction mixture of 20 µM pBSK+ DNA, free phenolic compounds (lanes 1–5) and Cu(II) complexes with phenolic compounds (lanes 6–10), incubated at RT for 30 min. The order of the columns in the gel: (p) control pDNA; (1–5) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM), (6–10) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM) + 5 µM Cu(II). Experimental conditions of all reactions were the same, so the observed differences in gels are related to structural differences of phenolic compounds. Due to the different solubility of phenolic compounds, individual phenolic compounds were used under different concentrations. (PhC = Phenolic compound; MYR = Myricetin; MOR = Morin; DHF = 3′,4′-dihydroxyflavone; TAX = Taxifolin; CUM = 4-hydroxycoumarin).

    Techniques Used: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation, Solubility

    Absorption spectra obtained by titration of free phenolic compound (20 μM) and phenolic compound in the presence of Cu(II) (Cu:phenolic compound = 1:2) with CT-DNA (0–50 μM) in a solution containing 5 mM Tris-HCl, 50 mM NaCl, pH 7.2 in the absence of CT-DNA (red line) and in the presence of an increasing concentration of CT-DNA (0–50 μM). The direction of the arrow indicates the shift in the absorbance after addition of the CT-DNA solution. Abbreviations: myricetin (MYR), morin (MOR), 3′,4′-dihydroxyflavone (DHF), taxifolin (TAX), 4-hydroxycoumarin (CUM).
    Figure Legend Snippet: Absorption spectra obtained by titration of free phenolic compound (20 μM) and phenolic compound in the presence of Cu(II) (Cu:phenolic compound = 1:2) with CT-DNA (0–50 μM) in a solution containing 5 mM Tris-HCl, 50 mM NaCl, pH 7.2 in the absence of CT-DNA (red line) and in the presence of an increasing concentration of CT-DNA (0–50 μM). The direction of the arrow indicates the shift in the absorbance after addition of the CT-DNA solution. Abbreviations: myricetin (MYR), morin (MOR), 3′,4′-dihydroxyflavone (DHF), taxifolin (TAX), 4-hydroxycoumarin (CUM).

    Techniques Used: Titration, Concentration Assay

    Gel electrophoregram of the interaction of phenolic compounds (PhC) and plasmid DNA in the presence of hydrogen peroxide (Fenton system). Electrophoretic profile (0.8% agarose gel) of DNA protection against oxidative damage in the reaction mixture (20 µL) containing Cu(II) ions, phenolic compounds, pBSK+ DNA, hydrogen peroxide and incubated at RT for 30 min. The order of the samples in the gel: (p) control pDNA; (C) Fenton like reaction containing pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 ; (1–8) Fenton like reaction + phenolic compound at concentrations of 5–500 µM (MYR, MOR, DHF) or 5–5000 µM (TAX, CUM); (L) 1 kb DNA standard (LIN) linearized pDNA.
    Figure Legend Snippet: Gel electrophoregram of the interaction of phenolic compounds (PhC) and plasmid DNA in the presence of hydrogen peroxide (Fenton system). Electrophoretic profile (0.8% agarose gel) of DNA protection against oxidative damage in the reaction mixture (20 µL) containing Cu(II) ions, phenolic compounds, pBSK+ DNA, hydrogen peroxide and incubated at RT for 30 min. The order of the samples in the gel: (p) control pDNA; (C) Fenton like reaction containing pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 ; (1–8) Fenton like reaction + phenolic compound at concentrations of 5–500 µM (MYR, MOR, DHF) or 5–5000 µM (TAX, CUM); (L) 1 kb DNA standard (LIN) linearized pDNA.

    Techniques Used: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation

    Relative densitometric quantification of band intensities of, at least, 3 agarose gels as illustrated in Figure 9 . Green colour corresponds to the SC form of plasmid DNA, red colour to the LIN form and orange colour to the OC form. Taxifolin and coumarin were tested up to a concentration of 5000 μM. p—SC form of pDNA, C —proportion of pDNA conformations formed in Fenton like reaction, 1–8—proportion of pDNA conformations formed in Fenton like reaction in the presence of phenolic compound. Standard deviations are expressed as error bars.
    Figure Legend Snippet: Relative densitometric quantification of band intensities of, at least, 3 agarose gels as illustrated in Figure 9 . Green colour corresponds to the SC form of plasmid DNA, red colour to the LIN form and orange colour to the OC form. Taxifolin and coumarin were tested up to a concentration of 5000 μM. p—SC form of pDNA, C —proportion of pDNA conformations formed in Fenton like reaction, 1–8—proportion of pDNA conformations formed in Fenton like reaction in the presence of phenolic compound. Standard deviations are expressed as error bars.

    Techniques Used: Plasmid Preparation, Concentration Assay

    8) Product Images from "Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study"

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22041619

    Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).
    Figure Legend Snippet: Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).

    Techniques Used: Concentration Assay

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    Solis BioDyne kb dna ladder
    Electrophoretic analysis of genomic <t>DNA</t> from biobanked frozen tumor samples. DNA (5 μL) was loaded on a 2% agarose gel and visualized by ethidium bromide staining. The gel shows the result of 10 representative samples. Compact bands of DNA were observed for all samples at a high molecular weight according to the <t>ladder.</t> The absence of smearing favors the absence of DNA degradation.
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    Electrophoretic analysis of genomic DNA from biobanked frozen tumor samples. DNA (5 μL) was loaded on a 2% agarose gel and visualized by ethidium bromide staining. The gel shows the result of 10 representative samples. Compact bands of DNA were observed for all samples at a high molecular weight according to the ladder. The absence of smearing favors the absence of DNA degradation.

    Journal: Frontiers in Medicine

    Article Title: Tumor Banks: A Quality Control Scheme Proposal

    doi: 10.3389/fmed.2019.00225

    Figure Lengend Snippet: Electrophoretic analysis of genomic DNA from biobanked frozen tumor samples. DNA (5 μL) was loaded on a 2% agarose gel and visualized by ethidium bromide staining. The gel shows the result of 10 representative samples. Compact bands of DNA were observed for all samples at a high molecular weight according to the ladder. The absence of smearing favors the absence of DNA degradation.

    Article Snippet: 1 kb DNA ladder from Solis Biodyne was used as molecular marker.

    Techniques: Agarose Gel Electrophoresis, Staining, Molecular Weight

    Gel electrophoregram of the interaction of phenolic compounds (PhC) and their Cu(II) complexes with plasmid DNA in the absence of hydrogen peroxide (non-Fenton system). Electrophoretic profile of agarose gel (0.8%) of the reaction mixture of 20 µM pBSK+ DNA, free phenolic compounds (lanes 1–5) and Cu(II) complexes with phenolic compounds (lanes 6–10), incubated at RT for 30 min. The order of the columns in the gel: (p) control pDNA; (1–5) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM), (6–10) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM) + 5 µM Cu(II). Experimental conditions of all reactions were the same, so the observed differences in gels are related to structural differences of phenolic compounds. Due to the different solubility of phenolic compounds, individual phenolic compounds were used under different concentrations. (PhC = Phenolic compound; MYR = Myricetin; MOR = Morin; DHF = 3′,4′-dihydroxyflavone; TAX = Taxifolin; CUM = 4-hydroxycoumarin).

    Journal: Molecules

    Article Title: A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

    doi: 10.3390/molecules24234335

    Figure Lengend Snippet: Gel electrophoregram of the interaction of phenolic compounds (PhC) and their Cu(II) complexes with plasmid DNA in the absence of hydrogen peroxide (non-Fenton system). Electrophoretic profile of agarose gel (0.8%) of the reaction mixture of 20 µM pBSK+ DNA, free phenolic compounds (lanes 1–5) and Cu(II) complexes with phenolic compounds (lanes 6–10), incubated at RT for 30 min. The order of the columns in the gel: (p) control pDNA; (1–5) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM), (6–10) pDNA + 5–500/5000 µM PhC (MYR, MOR, DHF/TAX, CUM) + 5 µM Cu(II). Experimental conditions of all reactions were the same, so the observed differences in gels are related to structural differences of phenolic compounds. Due to the different solubility of phenolic compounds, individual phenolic compounds were used under different concentrations. (PhC = Phenolic compound; MYR = Myricetin; MOR = Morin; DHF = 3′,4′-dihydroxyflavone; TAX = Taxifolin; CUM = 4-hydroxycoumarin).

    Article Snippet: The 1 kb DNA ladder was purchased from Solis BioDyne (Tartu, Estonia) and EcoRI restriction enzyme from Thermo Fisher Scientific (Vienna, Austria).

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation, Solubility

    Absorption spectra obtained by titration of free phenolic compound (20 μM) and phenolic compound in the presence of Cu(II) (Cu:phenolic compound = 1:2) with CT-DNA (0–50 μM) in a solution containing 5 mM Tris-HCl, 50 mM NaCl, pH 7.2 in the absence of CT-DNA (red line) and in the presence of an increasing concentration of CT-DNA (0–50 μM). The direction of the arrow indicates the shift in the absorbance after addition of the CT-DNA solution. Abbreviations: myricetin (MYR), morin (MOR), 3′,4′-dihydroxyflavone (DHF), taxifolin (TAX), 4-hydroxycoumarin (CUM).

    Journal: Molecules

    Article Title: A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

    doi: 10.3390/molecules24234335

    Figure Lengend Snippet: Absorption spectra obtained by titration of free phenolic compound (20 μM) and phenolic compound in the presence of Cu(II) (Cu:phenolic compound = 1:2) with CT-DNA (0–50 μM) in a solution containing 5 mM Tris-HCl, 50 mM NaCl, pH 7.2 in the absence of CT-DNA (red line) and in the presence of an increasing concentration of CT-DNA (0–50 μM). The direction of the arrow indicates the shift in the absorbance after addition of the CT-DNA solution. Abbreviations: myricetin (MYR), morin (MOR), 3′,4′-dihydroxyflavone (DHF), taxifolin (TAX), 4-hydroxycoumarin (CUM).

    Article Snippet: The 1 kb DNA ladder was purchased from Solis BioDyne (Tartu, Estonia) and EcoRI restriction enzyme from Thermo Fisher Scientific (Vienna, Austria).

    Techniques: Titration, Concentration Assay

    Gel electrophoregram of the interaction of phenolic compounds (PhC) and plasmid DNA in the presence of hydrogen peroxide (Fenton system). Electrophoretic profile (0.8% agarose gel) of DNA protection against oxidative damage in the reaction mixture (20 µL) containing Cu(II) ions, phenolic compounds, pBSK+ DNA, hydrogen peroxide and incubated at RT for 30 min. The order of the samples in the gel: (p) control pDNA; (C) Fenton like reaction containing pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 ; (1–8) Fenton like reaction + phenolic compound at concentrations of 5–500 µM (MYR, MOR, DHF) or 5–5000 µM (TAX, CUM); (L) 1 kb DNA standard (LIN) linearized pDNA.

    Journal: Molecules

    Article Title: A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

    doi: 10.3390/molecules24234335

    Figure Lengend Snippet: Gel electrophoregram of the interaction of phenolic compounds (PhC) and plasmid DNA in the presence of hydrogen peroxide (Fenton system). Electrophoretic profile (0.8% agarose gel) of DNA protection against oxidative damage in the reaction mixture (20 µL) containing Cu(II) ions, phenolic compounds, pBSK+ DNA, hydrogen peroxide and incubated at RT for 30 min. The order of the samples in the gel: (p) control pDNA; (C) Fenton like reaction containing pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 ; (1–8) Fenton like reaction + phenolic compound at concentrations of 5–500 µM (MYR, MOR, DHF) or 5–5000 µM (TAX, CUM); (L) 1 kb DNA standard (LIN) linearized pDNA.

    Article Snippet: The 1 kb DNA ladder was purchased from Solis BioDyne (Tartu, Estonia) and EcoRI restriction enzyme from Thermo Fisher Scientific (Vienna, Austria).

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Incubation

    Relative densitometric quantification of band intensities of, at least, 3 agarose gels as illustrated in Figure 9 . Green colour corresponds to the SC form of plasmid DNA, red colour to the LIN form and orange colour to the OC form. Taxifolin and coumarin were tested up to a concentration of 5000 μM. p—SC form of pDNA, C —proportion of pDNA conformations formed in Fenton like reaction, 1–8—proportion of pDNA conformations formed in Fenton like reaction in the presence of phenolic compound. Standard deviations are expressed as error bars.

    Journal: Molecules

    Article Title: A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3′,4′-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study

    doi: 10.3390/molecules24234335

    Figure Lengend Snippet: Relative densitometric quantification of band intensities of, at least, 3 agarose gels as illustrated in Figure 9 . Green colour corresponds to the SC form of plasmid DNA, red colour to the LIN form and orange colour to the OC form. Taxifolin and coumarin were tested up to a concentration of 5000 μM. p—SC form of pDNA, C —proportion of pDNA conformations formed in Fenton like reaction, 1–8—proportion of pDNA conformations formed in Fenton like reaction in the presence of phenolic compound. Standard deviations are expressed as error bars.

    Article Snippet: The 1 kb DNA ladder was purchased from Solis BioDyne (Tartu, Estonia) and EcoRI restriction enzyme from Thermo Fisher Scientific (Vienna, Austria).

    Techniques: Plasmid Preparation, Concentration Assay

    Electrophoretic profile of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu (II) ions and hydrogen peroxide. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; (1) pDNA + 5 µM CuCl 2 , 50 µM H 2 O 2 , (2–4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 plus (2) L-histidine, (3) DMSO, (4) SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Journal: International Journal of Molecular Sciences

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    doi: 10.3390/ijms22041619

    Figure Lengend Snippet: Electrophoretic profile of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu (II) ions and hydrogen peroxide. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; (1) pDNA + 5 µM CuCl 2 , 50 µM H 2 O 2 , (2–4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 plus (2) L-histidine, (3) DMSO, (4) SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Article Snippet: The 1 kb DNA ladder was from Solis BioDyne (Tartu, Estonia), ethidium bromide (EtBr) was from Serva (Heidelberg, Germany), EcoR1 restriction was from Thermoscientific (Rockford IL, USA), and the Isolate II Plasmid Kit from Bioline (Memphis TN, USA).

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation

    Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol, (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol; (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm −3 ).

    Journal: International Journal of Molecular Sciences

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    doi: 10.3390/ijms22041619

    Figure Lengend Snippet: Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol, (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol; (2) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm −3 ).

    Article Snippet: The 1 kb DNA ladder was from Solis BioDyne (Tartu, Estonia), ethidium bromide (EtBr) was from Serva (Heidelberg, Germany), EcoR1 restriction was from Thermoscientific (Rockford IL, USA), and the Isolate II Plasmid Kit from Bioline (Memphis TN, USA).

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation

    Electrophoretic profile of agarose gel (0.8%), 15 µM plasmid PBSK + DNA with Cu(II) ions, hydrogen peroxide and kaempferol incubated for 30 min. Samples were loaded in the gel lanes in the following order: (p) pDNA–control; (K) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 -control Cu-Fenton reaction; (1–8) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + (1) 5 µM kaempferol, (2) 10 µM kaempferol, (3) 50 µM kaempferol, (4) 100 µM kaempferol, (5) 200 µM kaempferol, (6) 300 µM kaempferol, (7) 400 µM kaempferol, (8) 500 µM kaempferol; (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Journal: International Journal of Molecular Sciences

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    doi: 10.3390/ijms22041619

    Figure Lengend Snippet: Electrophoretic profile of agarose gel (0.8%), 15 µM plasmid PBSK + DNA with Cu(II) ions, hydrogen peroxide and kaempferol incubated for 30 min. Samples were loaded in the gel lanes in the following order: (p) pDNA–control; (K) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 -control Cu-Fenton reaction; (1–8) pDNA + 5 µM CuCl 2 + 50 µM H 2 O 2 + (1) 5 µM kaempferol, (2) 10 µM kaempferol, (3) 50 µM kaempferol, (4) 100 µM kaempferol, (5) 200 µM kaempferol, (6) 300 µM kaempferol, (7) 400 µM kaempferol, (8) 500 µM kaempferol; (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease.

    Article Snippet: The 1 kb DNA ladder was from Solis BioDyne (Tartu, Estonia), ethidium bromide (EtBr) was from Serva (Heidelberg, Germany), EcoR1 restriction was from Thermoscientific (Rockford IL, USA), and the Isolate II Plasmid Kit from Bioline (Memphis TN, USA).

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Incubation

    Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).

    Journal: International Journal of Molecular Sciences

    Article Title: Antioxidant vs. Prooxidant Properties of the Flavonoid, Kaempferol, in the Presence of Cu(II) Ions: A ROS-Scavenging Activity, Fenton Reaction and DNA Damage Study

    doi: 10.3390/ijms22041619

    Figure Lengend Snippet: Relative viscosity of CT-DNA in buffer (5 mM TRIS, 50 mM NaCl, pH 7.2) influenced by increasing concentration of free kaempferol and Cu-kaempferol complex at 25 ± 0.1 °C compared to ethidium bromide (EtBr).

    Article Snippet: The 1 kb DNA ladder was from Solis BioDyne (Tartu, Estonia), ethidium bromide (EtBr) was from Serva (Heidelberg, Germany), EcoR1 restriction was from Thermoscientific (Rockford IL, USA), and the Isolate II Plasmid Kit from Bioline (Memphis TN, USA).

    Techniques: Concentration Assay

    Agarose electrophoresis of all 54 LR-PCR products for one DNA sample. Lane: 1–5 NR3C1 (2400 bp, 6301 bp, 5031 bp, 6208 bp, 2261 bp), 6–10 – NLRP1 (5287 bp, 7575 bp, 12504 bp, 5023 bp, 1088 bp), 11–13 – IPO13 (4357 bp, 6294 bp, 4762 bp), 14–19 – FKBP5 (2935 bp, 2534 bp, 6516 bp, 2523 bp, 7518 bp, 12540 bp), 20–23 – HSPA4 (1290 bp, 13489 bp, 7051 bp, 9368 bp), 24–29 – ABCB1 (3518 bp, 15887 bp, 16809 bp, 14483 bp, 17000 bp, 5967 bp), 30–32 – STIP1 (2224 bp, 6456 bp, 5400 bp), 33–35 – HSP90AA1 (1404 bp, 1238 bp, 5157 bp), 36 – IL1A (12577 bp), 37 – IL1B (10196 bp), 38 – IL2 (6505 bp), 39 – IL4 (10003 bp), 40 – CXCL8 (5000 bp), 41 – IL10 (6199 bp), 42 – NFKBI (5016 bp), 43 – JUN (4730 bp), 44 – MIF (2731 bp), 45 – TNF (8831 bp), 46–49 – MAPK14 (1910 bp, 7515 bp, 4900 bp, 13300 bp), 50–51 – CYP3A4 (10626 bp, 15518 bp), 52–54 – CYP3A5 (8829 bp, 7268 bp, 4910 bp), 1 kb – marker ladder 1000 bp. The gel images were obtained by trimming and color adjusting of the full-length gels in the IrfanView 4.44 program. The lanes numbering corresponds with the numbering of the amplified fragments in Tables III and IV

    Journal: Archives of Medical Science : AMS

    Article Title: NGS study of glucocorticoid response genes in inflammatory bowel disease patients

    doi: 10.5114/aoms.2019.84470

    Figure Lengend Snippet: Agarose electrophoresis of all 54 LR-PCR products for one DNA sample. Lane: 1–5 NR3C1 (2400 bp, 6301 bp, 5031 bp, 6208 bp, 2261 bp), 6–10 – NLRP1 (5287 bp, 7575 bp, 12504 bp, 5023 bp, 1088 bp), 11–13 – IPO13 (4357 bp, 6294 bp, 4762 bp), 14–19 – FKBP5 (2935 bp, 2534 bp, 6516 bp, 2523 bp, 7518 bp, 12540 bp), 20–23 – HSPA4 (1290 bp, 13489 bp, 7051 bp, 9368 bp), 24–29 – ABCB1 (3518 bp, 15887 bp, 16809 bp, 14483 bp, 17000 bp, 5967 bp), 30–32 – STIP1 (2224 bp, 6456 bp, 5400 bp), 33–35 – HSP90AA1 (1404 bp, 1238 bp, 5157 bp), 36 – IL1A (12577 bp), 37 – IL1B (10196 bp), 38 – IL2 (6505 bp), 39 – IL4 (10003 bp), 40 – CXCL8 (5000 bp), 41 – IL10 (6199 bp), 42 – NFKBI (5016 bp), 43 – JUN (4730 bp), 44 – MIF (2731 bp), 45 – TNF (8831 bp), 46–49 – MAPK14 (1910 bp, 7515 bp, 4900 bp, 13300 bp), 50–51 – CYP3A4 (10626 bp, 15518 bp), 52–54 – CYP3A5 (8829 bp, 7268 bp, 4910 bp), 1 kb – marker ladder 1000 bp. The gel images were obtained by trimming and color adjusting of the full-length gels in the IrfanView 4.44 program. The lanes numbering corresponds with the numbering of the amplified fragments in Tables III and IV

    Article Snippet: To identify the approximate size of amplified fragments, the 1 kb DNA Ladder (Solis Biodyne) was used.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Marker, Amplification