1×roche complete mini protease inhibitor cocktail  (Roche)

 
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    Structured Review

    Roche 1×roche complete mini protease inhibitor cocktail
    1×Roche Complete Mini Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1×roche complete mini protease inhibitor cocktail/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1×roche complete mini protease inhibitor cocktail - by Bioz Stars, 2020-04
    85/100 stars

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    Western Blot:

    Article Title: Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth
    Article Snippet: .. Western blot analysis Whole cell lysates were prepared in RIPA buffer supplemented with 10 mM β-glycerophosphate, 1 mM sodium orthovanadate, 10 mM NaF, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 1×Roche Complete Mini Protease Inhibitor Cocktail (Roche, Indianapolis, IN). .. To detect the level of IκBα in the cytosol and NF-κB p65 in the nuclear, cytosol and the nuclear fractions were extracted by using a kit from Nanjing Keygen (Nanjing, China).

    Protease Inhibitor:

    Article Title: Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth
    Article Snippet: .. Western blot analysis Whole cell lysates were prepared in RIPA buffer supplemented with 10 mM β-glycerophosphate, 1 mM sodium orthovanadate, 10 mM NaF, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 1×Roche Complete Mini Protease Inhibitor Cocktail (Roche, Indianapolis, IN). .. To detect the level of IκBα in the cytosol and NF-κB p65 in the nuclear, cytosol and the nuclear fractions were extracted by using a kit from Nanjing Keygen (Nanjing, China).

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  • 93
    Roche mini edta free protease inhibitor cocktail tablet
    Limited RNaseIII activity in E . histolytica trophozoite lysate. (A) Stem-loop structure of pre-miR122 dsRNA substrate used for RNaseIII activity assay. Mature miR-122/miR-122* duplex shown in red. Structure obtained from http://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000442 . (B) In vitro cleavage of [α- 32 P]ATP-labeled pre-miR122 by 10μl of whole cell lysate from E . histolytica trophozoites expressing Myc-EhRNaseIII. Reactions contained 3mM MgCl 2 , <t>20mM</t> potassium glutamate, ± 20mM <t>EDTA</t> and were incubated for 40, 80, or 120 minutes at 37°C. EDTA chelates the magnesium and inhibits RNaseIII activity. The dsRNA only sample contained no protein. The buffer only sample contained 10μl of lysate buffer (no protein). dsRNA and buffer only samples were incubated for 120 minutes at 37°C. Positive control reactions contained 1U of E . coli RNaseIII (Ambion). Asterisks indicate specific cleavage products– 50-60nt (*) and 20-21nt (**). The 40-50nt bands are non-specific products of the pre-miR122 cleavage.
    Mini Edta Free Protease Inhibitor Cocktail Tablet, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini edta free protease inhibitor cocktail tablet/product/Roche
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    mini edta free protease inhibitor cocktail tablet - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    99
    Roche complete mini protease inhibitor cocktail
    CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, <t>complete</t> digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.
    Complete Mini Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 350 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete mini protease inhibitor cocktail/product/Roche
    Average 99 stars, based on 350 article reviews
    Price from $9.99 to $1999.99
    complete mini protease inhibitor cocktail - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Roche complete mini edta free protease inhibitor cocktail tablets
    Extensive proteolytic processing in human platelets during storage. Platelets were stored under blood-banking conditions for 9 days with or without <t>EDTA-free</t> <t>cOmplete</t> protease inhibitor cocktail, and the platelet proteome from different days was characterized
    Complete Mini Edta Free Protease Inhibitor Cocktail Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete mini edta free protease inhibitor cocktail tablets/product/Roche
    Average 94 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    complete mini edta free protease inhibitor cocktail tablets - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    99
    Roche protease inhibitors
    Extensive proteolytic processing in human platelets during storage. Platelets were stored under blood-banking conditions for 9 days with or without <t>EDTA-free</t> <t>cOmplete</t> protease inhibitor cocktail, and the platelet proteome from different days was characterized
    Protease Inhibitors, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitors/product/Roche
    Average 99 stars, based on 6503 article reviews
    Price from $9.99 to $1999.99
    protease inhibitors - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Limited RNaseIII activity in E . histolytica trophozoite lysate. (A) Stem-loop structure of pre-miR122 dsRNA substrate used for RNaseIII activity assay. Mature miR-122/miR-122* duplex shown in red. Structure obtained from http://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000442 . (B) In vitro cleavage of [α- 32 P]ATP-labeled pre-miR122 by 10μl of whole cell lysate from E . histolytica trophozoites expressing Myc-EhRNaseIII. Reactions contained 3mM MgCl 2 , 20mM potassium glutamate, ± 20mM EDTA and were incubated for 40, 80, or 120 minutes at 37°C. EDTA chelates the magnesium and inhibits RNaseIII activity. The dsRNA only sample contained no protein. The buffer only sample contained 10μl of lysate buffer (no protein). dsRNA and buffer only samples were incubated for 120 minutes at 37°C. Positive control reactions contained 1U of E . coli RNaseIII (Ambion). Asterisks indicate specific cleavage products– 50-60nt (*) and 20-21nt (**). The 40-50nt bands are non-specific products of the pre-miR122 cleavage.

    Journal: PLoS ONE

    Article Title: A Single RNaseIII Domain Protein from Entamoeba histolytica Has dsRNA Cleavage Activity and Can Help Mediate RNAi Gene Silencing in a Heterologous System

    doi: 10.1371/journal.pone.0133740

    Figure Lengend Snippet: Limited RNaseIII activity in E . histolytica trophozoite lysate. (A) Stem-loop structure of pre-miR122 dsRNA substrate used for RNaseIII activity assay. Mature miR-122/miR-122* duplex shown in red. Structure obtained from http://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000442 . (B) In vitro cleavage of [α- 32 P]ATP-labeled pre-miR122 by 10μl of whole cell lysate from E . histolytica trophozoites expressing Myc-EhRNaseIII. Reactions contained 3mM MgCl 2 , 20mM potassium glutamate, ± 20mM EDTA and were incubated for 40, 80, or 120 minutes at 37°C. EDTA chelates the magnesium and inhibits RNaseIII activity. The dsRNA only sample contained no protein. The buffer only sample contained 10μl of lysate buffer (no protein). dsRNA and buffer only samples were incubated for 120 minutes at 37°C. Positive control reactions contained 1U of E . coli RNaseIII (Ambion). Asterisks indicate specific cleavage products– 50-60nt (*) and 20-21nt (**). The 40-50nt bands are non-specific products of the pre-miR122 cleavage.

    Article Snippet: Dicing assays were conducted in 20μl volumes where 10μl of whole cell extract was incubated with 10,000 cpm of radiolabeled dsRNA (~2 ng/μl final concentration) in reaction buffer (150mM sucrose, 20mM potassium L-glutamate (Sigma), 20mM HEPES-KOH, pH 7.9, 3mM MgCl2 , 10 μg/ml leupeptin, 1 Complete Mini EDTA-free protease inhibitor cocktail tablet (Roche Diagnostics) for every 3ml of buffer) [ ] supplemented with 30U Protector RNase Inhibitor (Roche Diagnostics) and 1U/μl SUPERNase·IN RNase Inhibitor (Ambion) with or without 20mM EDTA for 2 hours at 37°C unless otherwise indicated.

    Techniques: Activity Assay, In Vitro, Labeling, Expressing, Incubation, Positive Control

    CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, complete digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.

    Journal: Wellcome Open Research

    Article Title: Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo

    doi: 10.12688/wellcomeopenres.12394.1

    Figure Lengend Snippet: CRISPR/Cas9 mutagenesis of lamc3 causes defects in parachordal chain (PAC) development. ( A ) Design of sgRNAs targeted to exon1 of the lamc3 gene. Each sgRNA targets a restriction endonuclease site used for genotyping and is upstream of a protospacer adjustment motif (PAM). ( B – D ) Cas9/sgRNA injected embryos (as labelled) at 50 hpf. ( B – D ) Brightfield images show no developmental defects or developmental delay. ( B′ – D′ ) Whole embryo images of Tg( fli1a :egfp) fluorescence area indicated by white dotted line is enlarged in ( B′′ – D′′ ) the PAC in each hemisegment is indicated with a red asterisk. ( E ) Quantification of Tg( fli1a :egfp) embryos at 50 hpf with PAC defects. P-values determined by Fisher’s exact Two-tailed test compared to Cas9 alone controls. ( F – I ) 2% agarose genotyping gels with 100 bp ladder. ( F ) Schematic diagram of the region amplified by PCR contains two SphI sites (orange), which would digest into three fragments of 83, 164 and 263 bp in length. Predicted mutation of one of these sites will produce two bands of 83 and 427 bp in length. A natural variant contains a SNP in the second SphI site, complete digestion of this variant would produce two bands of 164 and 346 bp in length. Mutation of the target SphI site would prevent digestion of the fragment. Digestion of the amplicon with Taq α I (blue) in wild type embryos will yield two fragments of 204 and 306 bp in size. ( G ) Restriction endonuclease digest of target region shows undigested mutant products (black asterisk) at a size of 427/510 bp in sgRNA1-injected embryos. ( H ) Fewer undigested mutant products (510 bp) were observed in sgRNA2-injected embryos. ( I ) Brightfield images sgRNA1/Cas9 injected embryo at 5 dpf shows severe oedema. ( J ) Genotyping of 3-month adult fish. A, anterior; b/bp, base pairs; d, days post fertilisation; D, dorsal; kb, kilobases; mpf, months post fertilisation; n, number of embryos; PAC, parachordal chain; PAM, protospacer adjustment motif; SBMO, splice-blocking morpholino; sgRNA, short guide RNA; U, undigested control;.

    Article Snippet: Frozen embryos were thawed and homogenised in 100 μl of protein extraction buffer (1% IGEPAL, 150 mM NaCl, 20 mM Tris pH 7.5, 2 mM EDTA, 50 mM NaF, 1mM sodium pyrophosphate) with 1× cOmplete Mini Protease Inhibitor Cocktail (1183615300, Roche) overnight at 4°C with rotation.

    Techniques: CRISPR, Mutagenesis, Injection, Fluorescence, Two Tailed Test, Amplification, Polymerase Chain Reaction, Variant Assay, Fluorescence In Situ Hybridization, Blocking Assay

    Extensive proteolytic processing in human platelets during storage. Platelets were stored under blood-banking conditions for 9 days with or without EDTA-free cOmplete protease inhibitor cocktail, and the platelet proteome from different days was characterized

    Journal: Blood

    Article Title: TAILS N-terminomics of human platelets reveals pervasive metalloproteinase-dependent proteolytic processing in storage

    doi: 10.1182/blood-2014-04-569640

    Figure Lengend Snippet: Extensive proteolytic processing in human platelets during storage. Platelets were stored under blood-banking conditions for 9 days with or without EDTA-free cOmplete protease inhibitor cocktail, and the platelet proteome from different days was characterized

    Article Snippet: For broad protease inhibition, cOmplete mini EDTA-free protease inhibitor cocktail tablets (1 tablet/10 mL) (Roche) were used.

    Techniques: Protease Inhibitor

    Examples of proteolytic processing identified by TAILS in stored platelets. Platelets were stored under blood-banking conditions for 9 days with or without EDTA-free cOmplete protease inhibitor cocktail, and the platelet proteome from different days was

    Journal: Blood

    Article Title: TAILS N-terminomics of human platelets reveals pervasive metalloproteinase-dependent proteolytic processing in storage

    doi: 10.1182/blood-2014-04-569640

    Figure Lengend Snippet: Examples of proteolytic processing identified by TAILS in stored platelets. Platelets were stored under blood-banking conditions for 9 days with or without EDTA-free cOmplete protease inhibitor cocktail, and the platelet proteome from different days was

    Article Snippet: For broad protease inhibition, cOmplete mini EDTA-free protease inhibitor cocktail tablets (1 tablet/10 mL) (Roche) were used.

    Techniques: Protease Inhibitor