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Avanti Polar 1 2 dioleoyl sn glycero 3 phosphoserine dops
1 2 Dioleoyl Sn Glycero 3 Phosphoserine Dops, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 2 dioleoyl sn glycero 3 phosphoserine dops/product/Avanti Polar
Average 99 stars, based on 6 article reviews
Price from $9.99 to $1999.99
1 2 dioleoyl sn glycero 3 phosphoserine dops - by Bioz Stars, 2020-02
99/100 stars

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Related Articles

Recombinant:

Article Title: Endogenous Thrombin Potential Changes during the First Cycle of Oral Contraceptive Use
Article Snippet: The phospholipids 1,2-dioleoyl-sn-glycero-3-phosphCOCholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were from Avanti Polar Lipids (Alabaster, AL, USA). .. Recombinant tissue factor (Innovin) was purchased from Siemens Healthcare (Marburg, Germany).

Size-exclusion Chromatography:

Article Title: Oligomerisation of Synaptobrevin-2 Studied by Native Mass Spectrometry and Chemical Cross-Linking
Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS) and cholesterol (Avanti Polar Lipids) were mixed in chloroform/methanol (2:1 ( v / v )) at a molar ratio of 5:2:2:1 and dried using a rotary evaporator [ ]. .. Detergent removal and spontaneous proteoliposome formation was achieved by size exclusion chromatography using a self-packed Sephadex G25 Superfine column (5/100 mm, bed volume 2 mL) on an Äkta Pure system (GE Healthcare) equilibrated in 20 mM HEPES, pH 7.4, 150 mM KCl, and 0.1 mM TCEP.

Concentration Assay:

Article Title: Comparison of VILIP-1 and VILIP-3 Binding to Phospholipid Monolayers
Article Snippet: 1,2-Dioleoyl-sn-glycero- 3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero- 3-phosphoserine (DOPS), 1,2-dimyristoyl-sn-glycero- 3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero- 3-phosphoserine (DMPS) were purchased from Avanti Polar Lipids (Coger, France). .. The phospholipid solutions were prepared by mixing the desired amount of DMPS/DMPC or DOPS/DOPC at 3:1 or 1:3 molar ratio dissolved in chloroform solution at a concentration of 1 mg/mL.

Article Title: Effects of Inhalable Particulate Matter on Blood Coagulation
Article Snippet: The composition of the phospholipid mixture was as follows: 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoetanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (Avanti Polar Lipids Inc., Alabaster, Alabama) in the proportion of 20/20/60 (M/M). .. Testing was also performed in the presence of soluble rabbit thrombomodulin (ICN Biomedicals, Aurora, Ohio) as activator of protein C added in the reaction mixture at a final concentration of 4 nM.

Article Title: Oligomerisation of Synaptobrevin-2 Studied by Native Mass Spectrometry and Chemical Cross-Linking
Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS) and cholesterol (Avanti Polar Lipids) were mixed in chloroform/methanol (2:1 ( v / v )) at a molar ratio of 5:2:2:1 and dried using a rotary evaporator [ ]. .. The dried lipid film was hydrated in 20 mM HEPES, pH 7.4, 150 mM KCl, 0.1 mM TCEP, and 5% ( w / v ) sodium cholate yielding multilamellar vesicles with a total lipid concentration of 15 mM.

Generated:

Article Title: Effects of Inhalable Particulate Matter on Blood Coagulation
Article Snippet: The composition of the phospholipid mixture was as follows: 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoetanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (Avanti Polar Lipids Inc., Alabaster, Alabama) in the proportion of 20/20/60 (M/M). .. Continuous registration of the generated thrombin was achieved with a fluorogenic synthetic substrate (Z-Gly-Gly-Arg-AMC HCl, Bachem, Switzerland) added to the test system at a final concentration of 417 μM.

Article Title: C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity
Article Snippet: Reagents l -α-Phosphatidylcholine (egg, chicken; ePC), DOPC, 1,2-dioleoyl-sn -glycero-3-phosphoserine (DOPS), PI4P, PI, and NBD-PS were purchased from Avanti Polar lipids (Alabaster, AL). .. Rho 1D4 antibody used for preparation of immunoaffinity columns was generated in-house ( ; ) and purchased from UBC through Flintbox ( www.rho1d4.com/ ); primary antibodies against calnexin, actin, and tubulin were from Abcam; fluorescent-tagged secondary antibodies for immunofluorescence imaging were from Molecular Probes; and anti-Cdc50-7F4 (CDC50A) primary antibodies used in Western blots and immunofluorescence analysis were raised in-house ( ).

SPR Assay:

Article Title: A Novel Heparin-dependent Inhibitor of Activated Protein C That Potentiates Consumptive Coagulopathy in Russell's Viper Envenomation *
Article Snippet: Synthetic phospholipids 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl- sn -glycero-3-phosphoserine (DOPS) were bought from Avanti Polar Lipids. .. The CM5 sensor chip for surface plasmon resonance (SPR) analysis was purchased from GE Healthcare.

Enzyme-linked Immunosorbent Assay:

Article Title: Effects of Inhalable Particulate Matter on Blood Coagulation
Article Snippet: Tissue plasminogen activator (t-PA), D-dimer and C- Reactive Protein (CRP) were determined using commercially available ELISA assays (ELISA-Zymutest, HYPHEN BioMed). .. The composition of the phospholipid mixture was as follows: 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoetanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (Avanti Polar Lipids Inc., Alabaster, Alabama) in the proportion of 20/20/60 (M/M).

Activation Assay:

Article Title: Effects of Inhalable Particulate Matter on Blood Coagulation
Article Snippet: The test was based on the activation of coagulation in platelet-poor plasma with calcium chloride with or without tissue factor and phospholipids as exogenous triggers of blood coagulation. .. The composition of the phospholipid mixture was as follows: 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoetanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (Avanti Polar Lipids Inc., Alabaster, Alabama) in the proportion of 20/20/60 (M/M).

Incubation:

Article Title: Oligomerisation of Synaptobrevin-2 Studied by Native Mass Spectrometry and Chemical Cross-Linking
Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS) and cholesterol (Avanti Polar Lipids) were mixed in chloroform/methanol (2:1 ( v / v )) at a molar ratio of 5:2:2:1 and dried using a rotary evaporator [ ]. .. Syb(1-116) in 1% chaps was added at a lipid to protein ratio of 300:1 ( n / n ) and incubated for 1 h at 25 °C in a rotary evaporator without evaporation.

Coagulation:

Article Title: Effects of Inhalable Particulate Matter on Blood Coagulation
Article Snippet: The test was based on the activation of coagulation in platelet-poor plasma with calcium chloride with or without tissue factor and phospholipids as exogenous triggers of blood coagulation. .. The composition of the phospholipid mixture was as follows: 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoetanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) (Avanti Polar Lipids Inc., Alabaster, Alabama) in the proportion of 20/20/60 (M/M).

Article Title: A Novel Heparin-dependent Inhibitor of Activated Protein C That Potentiates Consumptive Coagulopathy in Russell's Viper Envenomation *
Article Snippet: Synthetic phospholipids 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl- sn -glycero-3-phosphoserine (DOPS) were bought from Avanti Polar Lipids. .. Normal coagulation control plasma and antithrombin/heparin cofactor II immune-depleted plasma were from HYPHEN Biomed and Enzyme Research Laboratories, respectively.

Imaging:

Article Title: C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity
Article Snippet: Reagents l -α-Phosphatidylcholine (egg, chicken; ePC), DOPC, 1,2-dioleoyl-sn -glycero-3-phosphoserine (DOPS), PI4P, PI, and NBD-PS were purchased from Avanti Polar lipids (Alabaster, AL). .. Rho 1D4 antibody used for preparation of immunoaffinity columns was generated in-house ( ; ) and purchased from UBC through Flintbox ( www.rho1d4.com/ ); primary antibodies against calnexin, actin, and tubulin were from Abcam; fluorescent-tagged secondary antibodies for immunofluorescence imaging were from Molecular Probes; and anti-Cdc50-7F4 (CDC50A) primary antibodies used in Western blots and immunofluorescence analysis were raised in-house ( ).

Evaporation:

Article Title: Oligomerisation of Synaptobrevin-2 Studied by Native Mass Spectrometry and Chemical Cross-Linking
Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS) and cholesterol (Avanti Polar Lipids) were mixed in chloroform/methanol (2:1 ( v / v )) at a molar ratio of 5:2:2:1 and dried using a rotary evaporator [ ]. .. Syb(1-116) in 1% chaps was added at a lipid to protein ratio of 300:1 ( n / n ) and incubated for 1 h at 25 °C in a rotary evaporator without evaporation.

Western Blot:

Article Title: C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity
Article Snippet: Reagents l -α-Phosphatidylcholine (egg, chicken; ePC), DOPC, 1,2-dioleoyl-sn -glycero-3-phosphoserine (DOPS), PI4P, PI, and NBD-PS were purchased from Avanti Polar lipids (Alabaster, AL). .. Rho 1D4 antibody used for preparation of immunoaffinity columns was generated in-house ( ; ) and purchased from UBC through Flintbox ( www.rho1d4.com/ ); primary antibodies against calnexin, actin, and tubulin were from Abcam; fluorescent-tagged secondary antibodies for immunofluorescence imaging were from Molecular Probes; and anti-Cdc50-7F4 (CDC50A) primary antibodies used in Western blots and immunofluorescence analysis were raised in-house ( ).

Mouse Assay:

Article Title: A Novel Heparin-dependent Inhibitor of Activated Protein C That Potentiates Consumptive Coagulopathy in Russell's Viper Envenomation *
Article Snippet: Synthetic phospholipids 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl- sn -glycero-3-phosphoserine (DOPS) were bought from Avanti Polar Lipids. .. ICR mice were purchased from BioLASCO and housed in a pathogen free environment.

Chromatin Immunoprecipitation:

Article Title: A Novel Heparin-dependent Inhibitor of Activated Protein C That Potentiates Consumptive Coagulopathy in Russell's Viper Envenomation *
Article Snippet: Synthetic phospholipids 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl- sn -glycero-3-phosphoserine (DOPS) were bought from Avanti Polar Lipids. .. The CM5 sensor chip for surface plasmon resonance (SPR) analysis was purchased from GE Healthcare.

Immunofluorescence:

Article Title: C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity
Article Snippet: Reagents l -α-Phosphatidylcholine (egg, chicken; ePC), DOPC, 1,2-dioleoyl-sn -glycero-3-phosphoserine (DOPS), PI4P, PI, and NBD-PS were purchased from Avanti Polar lipids (Alabaster, AL). .. Rho 1D4 antibody used for preparation of immunoaffinity columns was generated in-house ( ; ) and purchased from UBC through Flintbox ( www.rho1d4.com/ ); primary antibodies against calnexin, actin, and tubulin were from Abcam; fluorescent-tagged secondary antibodies for immunofluorescence imaging were from Molecular Probes; and anti-Cdc50-7F4 (CDC50A) primary antibodies used in Western blots and immunofluorescence analysis were raised in-house ( ).

Plasmid Preparation:

Article Title: Functional contacts between MPER and the anti-HIV-1 broadly neutralizing antibody 4E10 extend into the core of the membrane
Article Snippet: Vector pEVOL, encoding a tRNA synthetase suitable for the incorporation of the photoreactive amino acid, p BPA, was a gift from Prof. P. G. Schultz (The Scripps Research Institute, CA) [ ]. .. Lipids 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl- sn -glycero-3-phosphoserine (DOPS), sphingomyelin (SM), cholesterol (Chol), 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine (POPC), and 1,2-dioleoyl- sn -glycerol-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, Alabama).

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  • 99
    Avanti Polar dops
    Peptide-induced liposome leakage for negatively charged <t>(DOPC/DOPS,</t> DOPC/GM1, ‘mitochondria’) membranes, as well as zwitterionic DOPC membranes. *p
    Dops, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dops/product/Avanti Polar
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    dops - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    77
    Avanti Polar dops lipids
    Examples of how intensities of tube segments are recorded and quantified. Tube segments transiently diffuse into focus of the confocal microscope. An adaptive filament algorithm 41 was used to track the filaments and extract the intensity while they are in focus. By recording a number of images several different tube segments can be analyzed. ( A ) A GUV displaying a number of tube segments which are in focus (right panel). Left panel shows the fitted curves (red curves) which overlap with the tube segments which are in focus. The GUV membrane can similarly be quantified using the same strategy. Scale bar, 10 μm. ( B ) Enlarged image of a tube from ( A ) with the fitted curve as an overlay (red curve). ( C ) Intensity profile along the red curve in ( B ). The portion of the tube which is closest to the microscope focus corresponds to the maximum intensity value and is used in the following quantification of tube intensities. ( D ) Distribution of the maximum intensities from a number of tube segments from two different GUVs (images of GUV1 and GUV2 are shown to the right). The number of tube segments are N seg = 31 (GUV1) and N seg = 33 (GUV2). ( E ) Distribution of tube intensities from a GUV containing YFP labeled I-BAR coated tubes ([I-BAR] = 2.9 μM). The red squares represent maximum tube intensities from the membrane channel (TR-DHPE) and the green circles corresponds to the equivalent signal from the YFP labeled I-BAR ( N seg = 64). To the right are shown confocal images of the YFP labeled I-BAR (green) and membrane (red). Membrane composition is <t>DOPC:DOPS:TR-DHPE</t> 59.7:40:0.3.
    Dops Lipids, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dops lipids/product/Avanti Polar
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dops lipids - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    78
    Avanti Polar l α dioleoyl phosphatidylserine dops
    Physical stability of SN-38 liposome preparations. Notes: ( A ) Optical density measurements of SN-38 liposome suspensions. The measurements were conducted for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples immediately after preparation, and also 24, 72, and 168 h after preparation, from dark blue to light blue. Samples were stored at 4°C in the dark. ( B ) Size and ( C ) SN-38 retention rate during storage of lyophilized SN-38 loaded liposomes. The measurements were undertaken for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples after rehydration of freshly lyophilized samples and at 24 and 168 h after storage, from dark pink to light pink. The measurements were performed, from left to right, after rehydration of freshly lyophilized samples and 24, and 168 h after storage. Data correspond to mean values ± SD of, at least, three different experiments. Abbreviations: SLE, soy bean lipid extract; SN-38, irinotecan metabolite; DOC, <t>DSPC/DOPS/CHOL;</t> DSPC, L-α-distearoyl-phospathidylcholine; DOPS, <t>L-α-dioleoyl-phospathidylserine;</t> CHOL, cholesterol; EPC, egg yolk phosphatidylcholine; Zave, Z-average mean.
    L α Dioleoyl Phosphatidylserine Dops, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l α dioleoyl phosphatidylserine dops/product/Avanti Polar
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l α dioleoyl phosphatidylserine dops - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    Image Search Results


    Peptide-induced liposome leakage for negatively charged (DOPC/DOPS, DOPC/GM1, ‘mitochondria’) membranes, as well as zwitterionic DOPC membranes. *p

    Journal: Scientific Reports

    Article Title: Pronounced peptide selectivity for melanoma through tryptophan end-tagging

    doi: 10.1038/srep24952

    Figure Lengend Snippet: Peptide-induced liposome leakage for negatively charged (DOPC/DOPS, DOPC/GM1, ‘mitochondria’) membranes, as well as zwitterionic DOPC membranes. *p

    Article Snippet: DOPS (1,2-dioleoyl-sn -Glycero-3-phosphoserine, monosodium salt), DOPC (1,2-dioleoyl-sn -Glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn -Glycero-3-phoshoetanolamine), DOPI (1,2-dioleoyl-sn -Glycero-3-phosphoinositol, monosodium salt), CL (Cardiolipin; C18:1), and GM1 (Ganglioside GM1) were all from Avanti Polar Lipids (Alabaster, USA) and of > 99% purity.

    Techniques:

    z-potential of DOPC/DOPS (3/1 mol/mol), DOPC/GM1 (3/1 mol/mol), and ‘mitochondria’ (Mi), liposomes in the presence of GRR10W4 ( a ) and GRR10 ( b ) at the indicated concentrations. The point of zero potential is included to guide the eye. *p

    Journal: Scientific Reports

    Article Title: Pronounced peptide selectivity for melanoma through tryptophan end-tagging

    doi: 10.1038/srep24952

    Figure Lengend Snippet: z-potential of DOPC/DOPS (3/1 mol/mol), DOPC/GM1 (3/1 mol/mol), and ‘mitochondria’ (Mi), liposomes in the presence of GRR10W4 ( a ) and GRR10 ( b ) at the indicated concentrations. The point of zero potential is included to guide the eye. *p

    Article Snippet: DOPS (1,2-dioleoyl-sn -Glycero-3-phosphoserine, monosodium salt), DOPC (1,2-dioleoyl-sn -Glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn -Glycero-3-phoshoetanolamine), DOPI (1,2-dioleoyl-sn -Glycero-3-phosphoinositol, monosodium salt), CL (Cardiolipin; C18:1), and GM1 (Ganglioside GM1) were all from Avanti Polar Lipids (Alabaster, USA) and of > 99% purity.

    Techniques:

    Ellipsometry results on the adsorption of GRR10 and GRR10W4N at supported lipid membranes. ( a ) Effect of negatively charged DOPS and ganglioside GM1 content on the adsorption of GRR10W4N to DOPC/DOPS (left) and DOPC/GM1 (right) membranes. ( b ) Comparison of GRR10 and GRR10W4 adsorption to DOPC/DOPS and DOPC/GM1 membranes ( c ) Comparison of GRR10W4 adsorption to different negatively charged membranes of relevance to cancer cell uptake, as well as zwitterionic DOPC membranes. *p

    Journal: Scientific Reports

    Article Title: Pronounced peptide selectivity for melanoma through tryptophan end-tagging

    doi: 10.1038/srep24952

    Figure Lengend Snippet: Ellipsometry results on the adsorption of GRR10 and GRR10W4N at supported lipid membranes. ( a ) Effect of negatively charged DOPS and ganglioside GM1 content on the adsorption of GRR10W4N to DOPC/DOPS (left) and DOPC/GM1 (right) membranes. ( b ) Comparison of GRR10 and GRR10W4 adsorption to DOPC/DOPS and DOPC/GM1 membranes ( c ) Comparison of GRR10W4 adsorption to different negatively charged membranes of relevance to cancer cell uptake, as well as zwitterionic DOPC membranes. *p

    Article Snippet: DOPS (1,2-dioleoyl-sn -Glycero-3-phosphoserine, monosodium salt), DOPC (1,2-dioleoyl-sn -Glycero-3-phosphocholine), DOPE (1,2-dioleoyl-sn -Glycero-3-phoshoetanolamine), DOPI (1,2-dioleoyl-sn -Glycero-3-phosphoinositol, monosodium salt), CL (Cardiolipin; C18:1), and GM1 (Ganglioside GM1) were all from Avanti Polar Lipids (Alabaster, USA) and of > 99% purity.

    Techniques: Adsorption

    Normalized temporal correlation function for mode n = 5 for proteoGUVs of DOPC/DOPS/Chol/ATPase of similar reduced membrane tension. ( A ) Nonactive membranes in the absence of ATP conforming to a monoexponential form of the short-time decay (solid line).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

    doi: 10.1073/pnas.1209909109

    Figure Lengend Snippet: Normalized temporal correlation function for mode n = 5 for proteoGUVs of DOPC/DOPS/Chol/ATPase of similar reduced membrane tension. ( A ) Nonactive membranes in the absence of ATP conforming to a monoexponential form of the short-time decay (solid line).

    Article Snippet: DOPC (1,2-dioleyl- sn -glycero-3-phosphocholine), DOPS (1,2-dioleyl- sn -glycero-3-phosphoserine), and cholesterol were purchased from Avanti Polar Lipids and used without further purification.

    Techniques:

    For a proteoGUV of DOPC/DOPS/Chol/ATPase, a radius of 12.97 μm in the presence of 1 mM of ATP is shown: ( A ) Experimental distribution of the Fourier amplitudes of the contour fluctuations; α 5 or β 5 . ( B ) Plot of the normalized temporal

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

    doi: 10.1073/pnas.1209909109

    Figure Lengend Snippet: For a proteoGUV of DOPC/DOPS/Chol/ATPase, a radius of 12.97 μm in the presence of 1 mM of ATP is shown: ( A ) Experimental distribution of the Fourier amplitudes of the contour fluctuations; α 5 or β 5 . ( B ) Plot of the normalized temporal

    Article Snippet: DOPC (1,2-dioleyl- sn -glycero-3-phosphocholine), DOPS (1,2-dioleyl- sn -glycero-3-phosphoserine), and cholesterol were purchased from Avanti Polar Lipids and used without further purification.

    Techniques:

    Relaxation time as a function of mode number, n , for active membranes consisting of a proteoGUV of DOPC/DOPS/Chol/ATPase in the presence of ATP, (compare with ). ( A ) The initial relaxation time scales as τ 1 ∼ n –3.1 for modes

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

    doi: 10.1073/pnas.1209909109

    Figure Lengend Snippet: Relaxation time as a function of mode number, n , for active membranes consisting of a proteoGUV of DOPC/DOPS/Chol/ATPase in the presence of ATP, (compare with ). ( A ) The initial relaxation time scales as τ 1 ∼ n –3.1 for modes

    Article Snippet: DOPC (1,2-dioleyl- sn -glycero-3-phosphocholine), DOPS (1,2-dioleyl- sn -glycero-3-phosphoserine), and cholesterol were purchased from Avanti Polar Lipids and used without further purification.

    Techniques:

    Examples of how intensities of tube segments are recorded and quantified. Tube segments transiently diffuse into focus of the confocal microscope. An adaptive filament algorithm 41 was used to track the filaments and extract the intensity while they are in focus. By recording a number of images several different tube segments can be analyzed. ( A ) A GUV displaying a number of tube segments which are in focus (right panel). Left panel shows the fitted curves (red curves) which overlap with the tube segments which are in focus. The GUV membrane can similarly be quantified using the same strategy. Scale bar, 10 μm. ( B ) Enlarged image of a tube from ( A ) with the fitted curve as an overlay (red curve). ( C ) Intensity profile along the red curve in ( B ). The portion of the tube which is closest to the microscope focus corresponds to the maximum intensity value and is used in the following quantification of tube intensities. ( D ) Distribution of the maximum intensities from a number of tube segments from two different GUVs (images of GUV1 and GUV2 are shown to the right). The number of tube segments are N seg = 31 (GUV1) and N seg = 33 (GUV2). ( E ) Distribution of tube intensities from a GUV containing YFP labeled I-BAR coated tubes ([I-BAR] = 2.9 μM). The red squares represent maximum tube intensities from the membrane channel (TR-DHPE) and the green circles corresponds to the equivalent signal from the YFP labeled I-BAR ( N seg = 64). To the right are shown confocal images of the YFP labeled I-BAR (green) and membrane (red). Membrane composition is DOPC:DOPS:TR-DHPE 59.7:40:0.3.

    Journal: Scientific Reports

    Article Title: Dynamics of membrane nanotubes coated with I-BAR

    doi: 10.1038/srep30054

    Figure Lengend Snippet: Examples of how intensities of tube segments are recorded and quantified. Tube segments transiently diffuse into focus of the confocal microscope. An adaptive filament algorithm 41 was used to track the filaments and extract the intensity while they are in focus. By recording a number of images several different tube segments can be analyzed. ( A ) A GUV displaying a number of tube segments which are in focus (right panel). Left panel shows the fitted curves (red curves) which overlap with the tube segments which are in focus. The GUV membrane can similarly be quantified using the same strategy. Scale bar, 10 μm. ( B ) Enlarged image of a tube from ( A ) with the fitted curve as an overlay (red curve). ( C ) Intensity profile along the red curve in ( B ). The portion of the tube which is closest to the microscope focus corresponds to the maximum intensity value and is used in the following quantification of tube intensities. ( D ) Distribution of the maximum intensities from a number of tube segments from two different GUVs (images of GUV1 and GUV2 are shown to the right). The number of tube segments are N seg = 31 (GUV1) and N seg = 33 (GUV2). ( E ) Distribution of tube intensities from a GUV containing YFP labeled I-BAR coated tubes ([I-BAR] = 2.9 μM). The red squares represent maximum tube intensities from the membrane channel (TR-DHPE) and the green circles corresponds to the equivalent signal from the YFP labeled I-BAR ( N seg = 64). To the right are shown confocal images of the YFP labeled I-BAR (green) and membrane (red). Membrane composition is DOPC:DOPS:TR-DHPE 59.7:40:0.3.

    Article Snippet: Unless stated otherwise all GUVs were prepared from a lipid/dye mixture composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine, Avanti polar lipids), DOPS lipids (1,2-dioleoyl-sn-glycero-3-phosphoserine, Avanti Polar Lipids) and 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine-Texas Red was purchased from Invitrogen.

    Techniques: Microscopy, Labeling

    I-BAR domains from ABBA can form tubes pointing into Giant Unilamellar lipid Vesicles (GUVs) at high density or bind to existing tubes at lower density. ( A,B ) Schematic depiction showing I-BAR binding to tubes pointing inwards into the lumen of a GUV. ( C ) Incubation of a GUV with 2.3 μM I-BAR. Graph shows radial intensity plot of TR-DHPE signal (red) and YFP labeled I-BAR signal (green) from a GUV containing a number of inward pointing tubes as shown by the confocal images. ( D ) Incubation of a GUV with 290 nM I-BAR. Graph shows radial intensity of TR-DHPE signal (red) and YFP labeled I-BAR signal (green) from a GUV containing a number of inward pointing tubes as shown by the confocal images. Each intensity plotted in ( C,D ) is the average of all pixels with the same distance to the center of the GUV. The YFP intensities in ( C,D ) are normalized by the same constant. Membrane composition is DOPC:DOPS:TR-DHPE 59.7:40:0.3.

    Journal: Scientific Reports

    Article Title: Dynamics of membrane nanotubes coated with I-BAR

    doi: 10.1038/srep30054

    Figure Lengend Snippet: I-BAR domains from ABBA can form tubes pointing into Giant Unilamellar lipid Vesicles (GUVs) at high density or bind to existing tubes at lower density. ( A,B ) Schematic depiction showing I-BAR binding to tubes pointing inwards into the lumen of a GUV. ( C ) Incubation of a GUV with 2.3 μM I-BAR. Graph shows radial intensity plot of TR-DHPE signal (red) and YFP labeled I-BAR signal (green) from a GUV containing a number of inward pointing tubes as shown by the confocal images. ( D ) Incubation of a GUV with 290 nM I-BAR. Graph shows radial intensity of TR-DHPE signal (red) and YFP labeled I-BAR signal (green) from a GUV containing a number of inward pointing tubes as shown by the confocal images. Each intensity plotted in ( C,D ) is the average of all pixels with the same distance to the center of the GUV. The YFP intensities in ( C,D ) are normalized by the same constant. Membrane composition is DOPC:DOPS:TR-DHPE 59.7:40:0.3.

    Article Snippet: Unless stated otherwise all GUVs were prepared from a lipid/dye mixture composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine, Avanti polar lipids), DOPS lipids (1,2-dioleoyl-sn-glycero-3-phosphoserine, Avanti Polar Lipids) and 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine-Texas Red was purchased from Invitrogen.

    Techniques: Binding Assay, Incubation, Labeling

    Short membrane nanotubes coated with high density of I-BAR are semi-flexible with L p ~ L and do not move laterally on the GUV membrane. ( A ) From top to bottom panel, (i) example of a tube fixed on the GUV membrane (ii) the corresponding maximum projection of the intensity of a series of images of the same tube (iii) the corresponding average intensity from the entire time series and (iv) line profile parallel to the GUV membrane at a distance corresponding to L /3 from the GUV membrane. ( B–D ) More examples of short tubes plotted in the same sequence as in ( A ). ( E ) Theoretical calculation of the probability of a fluctuating rod which is fixed at one end. Top image shows a rod which has L p / L = 20, in the middle image L p /L = 1.7 and in the bottom image L p / L = 0.7. x and y axes range from 0 to L and –L to L, respectively. The graph below the images corresponds to line profiles along the depicted lines in the three images, L p / L = 20 (blue), L p / L = 1.7 (green) and L p / L = 0.7 (red). All images in ( A–D ) present intensities from YFP labeled I-BAR. Membrane composition DOPC:DOPS:TR-DHPE 59.7:40:0.3. The figure shows representative examples of the (N short > 100) short tubes observed in this work. All scale bars are 2 μm.

    Journal: Scientific Reports

    Article Title: Dynamics of membrane nanotubes coated with I-BAR

    doi: 10.1038/srep30054

    Figure Lengend Snippet: Short membrane nanotubes coated with high density of I-BAR are semi-flexible with L p ~ L and do not move laterally on the GUV membrane. ( A ) From top to bottom panel, (i) example of a tube fixed on the GUV membrane (ii) the corresponding maximum projection of the intensity of a series of images of the same tube (iii) the corresponding average intensity from the entire time series and (iv) line profile parallel to the GUV membrane at a distance corresponding to L /3 from the GUV membrane. ( B–D ) More examples of short tubes plotted in the same sequence as in ( A ). ( E ) Theoretical calculation of the probability of a fluctuating rod which is fixed at one end. Top image shows a rod which has L p / L = 20, in the middle image L p /L = 1.7 and in the bottom image L p / L = 0.7. x and y axes range from 0 to L and –L to L, respectively. The graph below the images corresponds to line profiles along the depicted lines in the three images, L p / L = 20 (blue), L p / L = 1.7 (green) and L p / L = 0.7 (red). All images in ( A–D ) present intensities from YFP labeled I-BAR. Membrane composition DOPC:DOPS:TR-DHPE 59.7:40:0.3. The figure shows representative examples of the (N short > 100) short tubes observed in this work. All scale bars are 2 μm.

    Article Snippet: Unless stated otherwise all GUVs were prepared from a lipid/dye mixture composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine, Avanti polar lipids), DOPS lipids (1,2-dioleoyl-sn-glycero-3-phosphoserine, Avanti Polar Lipids) and 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine-Texas Red was purchased from Invitrogen.

    Techniques: Sequencing, Labeling

    Membrane curvature sensing by I-BAR depends on protein density on the membrane. ( A ) Overlay of the membrane (red) and YFP labeled I-BAR (green) showing higher intensity of the protein inside the highly curved tube than on the nearly flat GUV membrane. The arrow shows an example of a tube connected with the GUV membrane. In subsequent images the tube disappears due to high mobility of the tube at low protein density. Protein concentration in solution is 290 nM. Scale bar, 10 μm. ( B ) Radial intensity of the membrane and YFP signal in a GUV incubated with YFP labeled I-BAR [I-BAR] = 2.3 μM. ( C ) Protein sorting as a function of distance from the center of the GUV. Sorting is defined as the ratio between the protein density on the tube and on the GUV membrane which can be calculated by using eq. 4 . ( D ) Example of radial intensity at lower density of [I-BAR] = 290 nM. ( E ) Corresponding sorting of the signals presented in ( D ). Each intensity plotted in ( B,D ) is the average of all pixels with the same distance to the center of the GUV. Confocal images in ( B,D ) show the overlay of the protein (green) and membrane (red). Membrane composition DOPC:DOPS:TR-DHPE 59.7:40:0.3. Data were recorded for 15 GUVs incubated with 290 nM of YFP labeled I-BAR and all showed similar level of sorting. At micromolar concentrations of the protein we never detected any sorting ( N = 59 GUVs).

    Journal: Scientific Reports

    Article Title: Dynamics of membrane nanotubes coated with I-BAR

    doi: 10.1038/srep30054

    Figure Lengend Snippet: Membrane curvature sensing by I-BAR depends on protein density on the membrane. ( A ) Overlay of the membrane (red) and YFP labeled I-BAR (green) showing higher intensity of the protein inside the highly curved tube than on the nearly flat GUV membrane. The arrow shows an example of a tube connected with the GUV membrane. In subsequent images the tube disappears due to high mobility of the tube at low protein density. Protein concentration in solution is 290 nM. Scale bar, 10 μm. ( B ) Radial intensity of the membrane and YFP signal in a GUV incubated with YFP labeled I-BAR [I-BAR] = 2.3 μM. ( C ) Protein sorting as a function of distance from the center of the GUV. Sorting is defined as the ratio between the protein density on the tube and on the GUV membrane which can be calculated by using eq. 4 . ( D ) Example of radial intensity at lower density of [I-BAR] = 290 nM. ( E ) Corresponding sorting of the signals presented in ( D ). Each intensity plotted in ( B,D ) is the average of all pixels with the same distance to the center of the GUV. Confocal images in ( B,D ) show the overlay of the protein (green) and membrane (red). Membrane composition DOPC:DOPS:TR-DHPE 59.7:40:0.3. Data were recorded for 15 GUVs incubated with 290 nM of YFP labeled I-BAR and all showed similar level of sorting. At micromolar concentrations of the protein we never detected any sorting ( N = 59 GUVs).

    Article Snippet: Unless stated otherwise all GUVs were prepared from a lipid/dye mixture composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine, Avanti polar lipids), DOPS lipids (1,2-dioleoyl-sn-glycero-3-phosphoserine, Avanti Polar Lipids) and 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine-Texas Red was purchased from Invitrogen.

    Techniques: Labeling, Protein Concentration, Incubation

    Physical stability of SN-38 liposome preparations. Notes: ( A ) Optical density measurements of SN-38 liposome suspensions. The measurements were conducted for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples immediately after preparation, and also 24, 72, and 168 h after preparation, from dark blue to light blue. Samples were stored at 4°C in the dark. ( B ) Size and ( C ) SN-38 retention rate during storage of lyophilized SN-38 loaded liposomes. The measurements were undertaken for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples after rehydration of freshly lyophilized samples and at 24 and 168 h after storage, from dark pink to light pink. The measurements were performed, from left to right, after rehydration of freshly lyophilized samples and 24, and 168 h after storage. Data correspond to mean values ± SD of, at least, three different experiments. Abbreviations: SLE, soy bean lipid extract; SN-38, irinotecan metabolite; DOC, DSPC/DOPS/CHOL; DSPC, L-α-distearoyl-phospathidylcholine; DOPS, L-α-dioleoyl-phospathidylserine; CHOL, cholesterol; EPC, egg yolk phosphatidylcholine; Zave, Z-average mean.

    Journal: International Journal of Nanomedicine

    Article Title: A novel microfluidic liposomal formulation for the delivery of the SN-38 camptothecin: characterization and in vitro assessment of its cytotoxic effect on two tumor cell lines

    doi: 10.2147/IJN.S166219

    Figure Lengend Snippet: Physical stability of SN-38 liposome preparations. Notes: ( A ) Optical density measurements of SN-38 liposome suspensions. The measurements were conducted for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples immediately after preparation, and also 24, 72, and 168 h after preparation, from dark blue to light blue. Samples were stored at 4°C in the dark. ( B ) Size and ( C ) SN-38 retention rate during storage of lyophilized SN-38 loaded liposomes. The measurements were undertaken for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples after rehydration of freshly lyophilized samples and at 24 and 168 h after storage, from dark pink to light pink. The measurements were performed, from left to right, after rehydration of freshly lyophilized samples and 24, and 168 h after storage. Data correspond to mean values ± SD of, at least, three different experiments. Abbreviations: SLE, soy bean lipid extract; SN-38, irinotecan metabolite; DOC, DSPC/DOPS/CHOL; DSPC, L-α-distearoyl-phospathidylcholine; DOPS, L-α-dioleoyl-phospathidylserine; CHOL, cholesterol; EPC, egg yolk phosphatidylcholine; Zave, Z-average mean.

    Article Snippet: Materials L-α-distearoyl-phosphatidylcholine (DSPC), egg yolk phosphatidylcholine (EPC), soy bean lipid extract (SLE), L-α-dioleoyl-phosphatidylserine (DOPS), and cholesterol (CHOL) were purchased from Avanti Polar Lipids (Birmingham, AL, USA).

    Techniques:

    Cytotoxic effect of SN-38 solubilized in dimethyl sulfoxide (DMSO) and encapsulated in liposomes. Notes: ( A ) Survival (%) of HeLa cells after treatment with SN-38 encapsulated in SLE liposomes at different molar ratios. ( B ) Survival (%) of HeLa cells after treatment with SN-38 encapsulated in liposomes with the compositions indicated, at the same lipid/drug molar ratio (20:1) and in DMSO solution. For ( A ) and ( B ), cells were incubated for 24 h with the drug and survival was evaluated 3, 24, and 48 h after drug removal by the MTT assay. Cell survival in the presence of the amount of blank liposomes or DMSO in the incubation medium, which would contain the amount of drug to provide all tested SN-38 concentrations, was always between 96% and 98%. Data correspond to mean values ± SD of at least three different experiments. Abbreviations: SLE, soy bean lipid extract; SN-38, irinotecan metabolite; DOC, DSPC/DOPS/CHOL; DSPC, L-α-distearoyl-phospathidylcholine; DOPS, L-α-dioleoyl-phosphatidylserine; CHOL, cholesterol; EPC, egg yolk phosphatidylcholine.

    Journal: International Journal of Nanomedicine

    Article Title: A novel microfluidic liposomal formulation for the delivery of the SN-38 camptothecin: characterization and in vitro assessment of its cytotoxic effect on two tumor cell lines

    doi: 10.2147/IJN.S166219

    Figure Lengend Snippet: Cytotoxic effect of SN-38 solubilized in dimethyl sulfoxide (DMSO) and encapsulated in liposomes. Notes: ( A ) Survival (%) of HeLa cells after treatment with SN-38 encapsulated in SLE liposomes at different molar ratios. ( B ) Survival (%) of HeLa cells after treatment with SN-38 encapsulated in liposomes with the compositions indicated, at the same lipid/drug molar ratio (20:1) and in DMSO solution. For ( A ) and ( B ), cells were incubated for 24 h with the drug and survival was evaluated 3, 24, and 48 h after drug removal by the MTT assay. Cell survival in the presence of the amount of blank liposomes or DMSO in the incubation medium, which would contain the amount of drug to provide all tested SN-38 concentrations, was always between 96% and 98%. Data correspond to mean values ± SD of at least three different experiments. Abbreviations: SLE, soy bean lipid extract; SN-38, irinotecan metabolite; DOC, DSPC/DOPS/CHOL; DSPC, L-α-distearoyl-phospathidylcholine; DOPS, L-α-dioleoyl-phosphatidylserine; CHOL, cholesterol; EPC, egg yolk phosphatidylcholine.

    Article Snippet: Materials L-α-distearoyl-phosphatidylcholine (DSPC), egg yolk phosphatidylcholine (EPC), soy bean lipid extract (SLE), L-α-dioleoyl-phosphatidylserine (DOPS), and cholesterol (CHOL) were purchased from Avanti Polar Lipids (Birmingham, AL, USA).

    Techniques: Incubation, MTT Assay

    SEM and TEM images. Notes: SEM ( A ) and TEM ( B ) images of a lyophilized preparation of SN-38-loaded EPC/DOPS liposomes. The bar is equivalent to 100 nm. Abbreviations: TEM, transmission electron microscopy; SEM, scanning electron microscopy; SN-38, irinotecan metabolite; EPC, egg yolk phosphatidylcholine; DOPS, L-α-dioleoyl-phosphatidylserine.

    Journal: International Journal of Nanomedicine

    Article Title: A novel microfluidic liposomal formulation for the delivery of the SN-38 camptothecin: characterization and in vitro assessment of its cytotoxic effect on two tumor cell lines

    doi: 10.2147/IJN.S166219

    Figure Lengend Snippet: SEM and TEM images. Notes: SEM ( A ) and TEM ( B ) images of a lyophilized preparation of SN-38-loaded EPC/DOPS liposomes. The bar is equivalent to 100 nm. Abbreviations: TEM, transmission electron microscopy; SEM, scanning electron microscopy; SN-38, irinotecan metabolite; EPC, egg yolk phosphatidylcholine; DOPS, L-α-dioleoyl-phosphatidylserine.

    Article Snippet: Materials L-α-distearoyl-phosphatidylcholine (DSPC), egg yolk phosphatidylcholine (EPC), soy bean lipid extract (SLE), L-α-dioleoyl-phosphatidylserine (DOPS), and cholesterol (CHOL) were purchased from Avanti Polar Lipids (Birmingham, AL, USA).

    Techniques: Transmission Electron Microscopy, Transmission Assay, Electron Microscopy