1 2 dioleoyl sn glycero 3 phosphoethanolamine dope  (Avanti Polar)

 
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    Structured Review

    Avanti Polar 1 2 dioleoyl sn glycero 3 phosphoethanolamine dope
    Chemical structures of the lipids used in the study. (A) <t>1,2-diphytanoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DPhPC) and 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS). (B) <t>1,2-dioleoyl-</t> sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoetha-nolamine (DOPE), and bis(monooleoylglycero)phosphate (S,R isomer) (DOBMP).
    1 2 Dioleoyl Sn Glycero 3 Phosphoethanolamine Dope, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dioleoyl sn glycero 3 phosphoethanolamine dope/product/Avanti Polar
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    1 2 dioleoyl sn glycero 3 phosphoethanolamine dope - by Bioz Stars, 2020-02
    99/100 stars

    Images

    1) Product Images from "Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity"

    Article Title: Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity

    Journal: Biochimica et biophysica acta. Biomembranes

    doi: 10.1016/j.bbamem.2018.08.011

    Chemical structures of the lipids used in the study. (A) 1,2-diphytanoyl- sn -glycero-3-phosphocholine (DPhPC) and 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS). (B) 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoetha-nolamine (DOPE), and bis(monooleoylglycero)phosphate (S,R isomer) (DOBMP).
    Figure Legend Snippet: Chemical structures of the lipids used in the study. (A) 1,2-diphytanoyl- sn -glycero-3-phosphocholine (DPhPC) and 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS). (B) 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoetha-nolamine (DOPE), and bis(monooleoylglycero)phosphate (S,R isomer) (DOBMP).

    Techniques Used:

    2) Product Images from "A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior"

    Article Title: A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S179806

    Schematic drawing of lipoplex assembly and radiolabeling procedures of the gene delivery nanosystem. Abbreviation: DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine.
    Figure Legend Snippet: Schematic drawing of lipoplex assembly and radiolabeling procedures of the gene delivery nanosystem. Abbreviation: DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine.

    Techniques Used: Radioactivity

    3) Product Images from "Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells"

    Article Title: Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S24447

    DSPE-PEG-RGD peptide conjugates ( A ), Schematic representation of synthesis of DSPE-PEG-RGD peptide conjugates ( B ) MALDI-TOF mass spectrometric analysis of the DSPE-PEG-RGD conjugate and parental DSPE-PEG maleimide, demonstrating an increase in mass from 2922.79 to 3741.69 after conjugation with the thiolated RGD peptide. This corresponds to one thiolated RGD peptide molecule conjugated to one DSPE-PEG maleimide molecule. Abbreviations: DSPE-PEG-RGD, Arg(R)-Gly(G)-Asp(D) motif peptide conjugated to 1,2-distearoyl- sn -glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000]; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight.
    Figure Legend Snippet: DSPE-PEG-RGD peptide conjugates ( A ), Schematic representation of synthesis of DSPE-PEG-RGD peptide conjugates ( B ) MALDI-TOF mass spectrometric analysis of the DSPE-PEG-RGD conjugate and parental DSPE-PEG maleimide, demonstrating an increase in mass from 2922.79 to 3741.69 after conjugation with the thiolated RGD peptide. This corresponds to one thiolated RGD peptide molecule conjugated to one DSPE-PEG maleimide molecule. Abbreviations: DSPE-PEG-RGD, Arg(R)-Gly(G)-Asp(D) motif peptide conjugated to 1,2-distearoyl- sn -glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000]; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight.

    Techniques Used: Conjugation Assay

    4) Product Images from "A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior"

    Article Title: A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S179806

    Schematic drawing of lipoplex assembly and radiolabeling procedures of the gene delivery nanosystem. Abbreviation: DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine.
    Figure Legend Snippet: Schematic drawing of lipoplex assembly and radiolabeling procedures of the gene delivery nanosystem. Abbreviation: DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine.

    Techniques Used: Radioactivity

    5) Product Images from "Simultaneous delivery of anti-miR21 with doxorubicin prodrug by mimetic lipoprotein nanoparticles for synergistic effect against drug resistance in cancer cells"

    Article Title: Simultaneous delivery of anti-miR21 with doxorubicin prodrug by mimetic lipoprotein nanoparticles for synergistic effect against drug resistance in cancer cells

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S122171

    Agarose gel electrophoresis analysis. ( A ) Gel retardation assay of complexation efficiency of anti-miR21 by NLS-Dox. Lane 1: free anti-miR21 control, lane 2–9: complexation at various N/P molar ratios. ( B ) Gel retardation assay of the incorporation efficiency of complexes into anionic DMPG liposomes composed, lane 1–8: encapsulation at varying DMPG:anti-miR21 weight ratios. ( C ) Gel retardation assay of the incorporation efficiency of complexes into anionic liposomes composed of DMPG–DOPE (1:1, m/m). ( D ) Gel retardation assay of the incorporation efficiency after the preparation of the HMNs followed by incubation of Apo AI with preformed DMPG–DOPE liposomes at varying DMPG:anti-miR21 weight ratios. Abbreviations: miR21, microRNA21; DMPG, 1,2-ditetradecanoyl- sn -glycero-3-phospho-(1′- rac -glycerol); DOPE, 1,2-dioleoyl- sn glycero-3-phosphoethanolamine; HMNs, high-density lipoprotein-mimicking nanoparticles.
    Figure Legend Snippet: Agarose gel electrophoresis analysis. ( A ) Gel retardation assay of complexation efficiency of anti-miR21 by NLS-Dox. Lane 1: free anti-miR21 control, lane 2–9: complexation at various N/P molar ratios. ( B ) Gel retardation assay of the incorporation efficiency of complexes into anionic DMPG liposomes composed, lane 1–8: encapsulation at varying DMPG:anti-miR21 weight ratios. ( C ) Gel retardation assay of the incorporation efficiency of complexes into anionic liposomes composed of DMPG–DOPE (1:1, m/m). ( D ) Gel retardation assay of the incorporation efficiency after the preparation of the HMNs followed by incubation of Apo AI with preformed DMPG–DOPE liposomes at varying DMPG:anti-miR21 weight ratios. Abbreviations: miR21, microRNA21; DMPG, 1,2-ditetradecanoyl- sn -glycero-3-phospho-(1′- rac -glycerol); DOPE, 1,2-dioleoyl- sn glycero-3-phosphoethanolamine; HMNs, high-density lipoprotein-mimicking nanoparticles.

    Techniques Used: Agarose Gel Electrophoresis, Electrophoretic Mobility Shift Assay, Incubation

    Related Articles

    Marker:

    Article Title: Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy
    Article Snippet: Primary antibody Iba1, a microglia marker, was purchased from Wako Pure Chemical Industries (Japan) and secondary antibody Alexa 594 was purchased from Invitrogen (Portugal). .. Control cationic lipids 3β -[N -(N ′,N ′-dimethylaminoethane)-carbamoyl]-cholesterol (DC-Chol) and 1,2-dimyristoyl-sn -glycero-3-ethylphosphocholine (EPC) [ ] and the neutral colipids cholesterol (Chol) and 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE) were obtained from Avanti Polar Lipids (Alabaster, AL, USA).

    Transfection:

    Article Title: Non-viral VEGF165 gene therapy – magnetofection of acoustically active magnetic lipospheres (‘magnetobubbles’) increases tissue survival in an oversized skin flap model
    Article Snippet: The cationic lipid being the major component of the cationic lipid transfection reagent Metafectene™ was kindly provided by Dr. Stephan König (Biontex GmbH, Martinsried, Germany). .. 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar lipids (Alabaster, AL, USA).

    Luciferase:

    Article Title: Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery
    Article Snippet: 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA. .. Luciferase assay kit and MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium, inner salt) were purchased from Promega (Madison, WI, USA).

    DNA Profiling:

    Article Title: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor
    Article Snippet: Tissue culture, reagents and antibodies Basal-like breast cancer cell-lines, MDA-MB-231 (purchased from ATCC) and HCC1954 (generous gift of Dr Andrew Tutt, King’s College London, UK), independently validated by STR DNA fingerprinting at The Institute of Cancer Research (London, UK), were maintained in DMEM and RPMI-1640, respectively (Life Technologies Ltd, Paisley, Uk) and tested to be mycoplasma free. .. 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and Cy5-NHS was from GE Healthcare (Amersham, UK).

    Fluorescence:

    Article Title: Lipid Modified Triblock PAMAM-Based Nanocarriers for siRNA Drug Co-Delivery
    Article Snippet: 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000](ammonium salt) (PEG-5K -DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were purchased from Avanti Polar Lipids (AL, USA). .. Geneticin (G-418), FAM-labeled negative control siRNA and silencer Cy-3-labeled GAPDH siRNA were purchased from Ambion Life Technologies Invitrogen (NY, USA). siRNA, targeting green fluorescence proteins (siGFP): 5'-AUGAACUUCAGGGUCAGCUdTdT-3' (sense), ( ) Non-targeting control siRNA (siNegative): 5'-AGUACUGCUUACGAUACGGdTdT-3' (sense).

    Synthesized:

    Article Title: Simultaneous delivery of anti-miR21 with doxorubicin prodrug by mimetic lipoprotein nanoparticles for synergistic effect against drug resistance in cancer cells
    Article Snippet: Materials 1,2-Ditetradecanoyl-sn -glycero-3-phospho-(1′-rac -glycerol) (DMPG) and 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). .. The NLS peptides (CPKKKRKV-NH2 ) used in this work were synthesized by China Peptides (Shanghai, China).

    Article Title: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor
    Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and Cy5-NHS was from GE Healthcare (Amersham, UK). .. N -(2-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)ethyl)-N,N -dimethyl-2,3-bis((Z )-octadec-9-enyloxy)propan-1-aminium (DODEG4) and N,N,N -trimethyl-2,3-bis((Z )-octadec-9-enyloxy)propan-1-aminium (DOTMA) , were synthesized as previously described.

    Article Title: Influence of the Lipid Anchor Motif of N-Ras on the Interaction with Lipid Membranes: A Surface Plasmon Resonance Study
    Article Snippet: Lipids (1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) (100%), 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) (100%), 1,2-dioleoyl- sn -glycero-3-(phospho-rac-(1-glycerol)) (sodium salt) (DOPG) (100%), and 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) (100%)) were purchased from Avanti Polar Lipids (Alabaster, AL), and cholesterol (Chol) (≥99%) was obtained from Sigma Aldrich (Deisenhofen, Germany). .. Six different N-Ras constructs with different lipid anchors and nucleotide loading (Far/Far (GDP), HD/Far (GDP), HD/HD (GDP), Stbut/Far (GDP), HD/Far (GppNHp, 5′-guanosyl-( β , γ -imido)-triphosphate)), and nonlipidated N-Ras (GDP)) were synthesized as previously described ( ).

    Multiple Displacement Amplification:

    Article Title: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor
    Article Snippet: Tissue culture, reagents and antibodies Basal-like breast cancer cell-lines, MDA-MB-231 (purchased from ATCC) and HCC1954 (generous gift of Dr Andrew Tutt, King’s College London, UK), independently validated by STR DNA fingerprinting at The Institute of Cancer Research (London, UK), were maintained in DMEM and RPMI-1640, respectively (Life Technologies Ltd, Paisley, Uk) and tested to be mycoplasma free. .. 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and Cy5-NHS was from GE Healthcare (Amersham, UK).

    Mouse Assay:

    Article Title: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor
    Article Snippet: Bone marrow macrophages were extracted from HCC1954 tumour bearing mice, cultured with macrophage colony stimulating factor-1 (GFM8-100, Cambridge Bioscience, UK) for 7 days before use in experiments. .. 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and Cy5-NHS was from GE Healthcare (Amersham, UK).

    Plasmid Preparation:

    Article Title: Non-viral VEGF165 gene therapy – magnetofection of acoustically active magnetic lipospheres (‘magnetobubbles’) increases tissue survival in an oversized skin flap model
    Article Snippet: The plasmid pcDNA3.1(+) EGFP encoding enhanced green fluorescent protein driven under CMV-promoter was kindly provided by Dr. Martina Anton (Institute of Experimental Oncology, Technische Universität München, Germany). .. 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar lipids (Alabaster, AL, USA).

    Article Title: Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery
    Article Snippet: 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA. .. Endotoxin-free plasmid purification kit was purchased from TIANGEN (Beijing, China).

    Article Title: A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior
    Article Snippet: 3,3′-Iminobis( N , N -dimethylpropylamine), Boc-Gly-OH, Fmoc-Lys(Boc)-OH, and 1-Iodododecane were purchased from Sigma-Aldrich Co. (St Louis, MO, USA); N , N ′- Diisopropylethylamine (DIPEA) and O-(7-Azabenzotriazol-1-yl)- N , N , N ′, N ′-tetramethyluronium hexafluorophosphate (HATU) were purchased from Chem-Impex International, Inc (Wood Dale, IL, USA); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar Lipids, Inc (Alabaster, AL, USA) and p-SCN-Bn-Deferoxamine (deferoxamine) was purchased from Macrocyclics, Inc (Cat. No: B-705) (Dallas, TX, USA). .. The construction of the plasmid pGTh-CMV.

    Article Title: A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior
    Article Snippet: Materials 3,3′-Iminobis(N ,N -dimethylpropylamine), Boc-Gly-OH, Fmoc-Lys(Boc)-OH, and 1-Iodododecane were purchased from Sigma-Aldrich Co. (St Louis, MO, USA); N ,N ′- Diisopropylethylamine (DIPEA) and O-(7-Azabenzotriazol-1-yl)-N ,N ,N ′,N ′-tetramethyluronium hexafluorophosphate (HATU) were purchased from Chem-Impex International, Inc (Wood Dale, IL, USA); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar Lipids, Inc (Alabaster, AL, USA) and p-SCN-Bn-Deferoxamine (deferoxamine) was purchased from Macrocyclics, Inc (Cat. No: B-705) (Dallas, TX, USA). .. The construction of the plasmid pGTh-CMV.

    Negative Control:

    Article Title: Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells
    Article Snippet: Chemicals A negative control siRNA or FAM-labeled negative control siRNA (having a molecular mass of approximately 13.5 kDa, sense: FAM-5′-UUC-UCC-GAA-CGU-GUC-ACG- UTT-3′ and antisense: 5′-ACG-UGA-CAC-GUU-CGG- AGA-ATT-3′) were purchased from MDBio Inc (Taipei, Taiwan). .. All of the lipid materials used, 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-cholesterol), 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE), 1,2-distearoyl-sn -glycero-3-phosphoethanolamine-N-[carboxy (polyethylene glycol)-2000] [DSPE-PEG(2000) carboxy], and 1,2-distearoyl-sn -glycero-3-phosphoethanolamine- N-[maleimide (polyethylene glycol)-2000] [DSPE-PEG(2000) maleimide] were obtained from Avanti Polar Lipids (Alabaster, AL).

    Article Title: Lipid Modified Triblock PAMAM-Based Nanocarriers for siRNA Drug Co-Delivery
    Article Snippet: 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000](ammonium salt) (PEG-5K -DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were purchased from Avanti Polar Lipids (AL, USA). .. Geneticin (G-418), FAM-labeled negative control siRNA and silencer Cy-3-labeled GAPDH siRNA were purchased from Ambion Life Technologies Invitrogen (NY, USA). siRNA, targeting green fluorescence proteins (siGFP): 5'-AUGAACUUCAGGGUCAGCUdTdT-3' (sense), ( ) Non-targeting control siRNA (siNegative): 5'-AGUACUGCUUACGAUACGGdTdT-3' (sense).

    Cell Culture:

    Article Title: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor
    Article Snippet: Bone marrow macrophages were extracted from HCC1954 tumour bearing mice, cultured with macrophage colony stimulating factor-1 (GFM8-100, Cambridge Bioscience, UK) for 7 days before use in experiments. .. 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and Cy5-NHS was from GE Healthcare (Amersham, UK).

    Purification:

    Article Title: Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery
    Article Snippet: Anhydrous chloroform and dichloromethane were dried and purified under nitrogen by using standard methods and were distilled immediately before use. .. 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA.

    Article Title: Improvement of Immunogenicity of Meningococcal Lipooligosaccharide by Coformulation with Lipidated Transferrin-Binding Protein B in Liposomes: Implications for Vaccine Development
    Article Snippet: Small cationic liposomes were prepared by the Bangham method ( ) from ethyl-DOPC and 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) (Avanti Polar Lipids, Inc., Alabaster, AL) in a 3:2 molar ratio, and LOS was incorporated using a detergent-removal method. .. Liposomes comprising increasing amounts of LOS, ranging from a 25:1 to 400:1 lipid/LOS molar ratio were then prepared by adding various amounts of a sterile solution of purified LOS at 1 mg/ml in 10 mM Tris–100 mM OG, pH 7.0, to the mixed ethyl-DOPC/DOPE/OG micelles followed by OG removal by dialysis against 10 mM Tris, pH 7.0, under aseptic conditions.

    Article Title: Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity
    Article Snippet: PA63 was prepared from PA83 using furin [ ] or purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) in the purified form. .. 1,2-Diphytanoyl- sn -glycero-3-phosphocholine (DPhPC), 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS), 1, 2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), bis (monooleoylglycero)phosphate (S,R isomer) (DOBMP), 1,2-diphytanoyl- sn -glycero-3-phosphoethanolamine (DPhPE), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), and bis(monomyristoylglycero)phosphate (S,R isomer) (DMBMP) lipids were purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA (all in chloroform).

    Article Title: A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats
    Article Snippet: Materials 1,2-dioleoyl-sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE), L -α-phosphatidylinositol (liver PI), DOPS, PIP2 , BSM, di-stearoyl phosphatidyl ethanolamine-PEG(2000)-biotin and cholesterol were purchased from Avanti Polar Lipids. .. Clathrin was extracted from fresh porcine brains and purified as described in ref. .

    Produced:

    Article Title: Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity
    Article Snippet: D512K and K245G-R252N bicomponent octameric variant of PA was produced as described in ref. [ ]. .. 1,2-Diphytanoyl- sn -glycero-3-phosphocholine (DPhPC), 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS), 1, 2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), bis (monooleoylglycero)phosphate (S,R isomer) (DOBMP), 1,2-diphytanoyl- sn -glycero-3-phosphoethanolamine (DPhPE), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), and bis(monomyristoylglycero)phosphate (S,R isomer) (DMBMP) lipids were purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA (all in chloroform).

    Modification:

    Article Title: Cationic Liposomes Carrying siRNA: Impact of Lipid Composition on Physicochemical Properties, Cytotoxicity and Endosomal Escape
    Article Snippet: 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and 3ß-[ N -( N ′, N ′-dimethylaminoethane)-carbamoyl]cholesterol hydrochloride (DC-Chol) and cholesterol (Chol) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). .. Dulbecco’s Modified Eagle Medium (DMEM), Fetal Bovine Serum (FBS), MEM non-essential amino acids, Penicillin Streptomycin (PenStrep® ), AmphotericinB (Fungizone® ), Trypsine-EDTA and Opti-MEM® were purchased from Gibco (Invitrogen, Rockville, MD, USA).

    Construct:

    Article Title: Influence of the Lipid Anchor Motif of N-Ras on the Interaction with Lipid Membranes: A Surface Plasmon Resonance Study
    Article Snippet: Lipids (1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) (100%), 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) (100%), 1,2-dioleoyl- sn -glycero-3-(phospho-rac-(1-glycerol)) (sodium salt) (DOPG) (100%), and 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) (100%)) were purchased from Avanti Polar Lipids (Alabaster, AL), and cholesterol (Chol) (≥99%) was obtained from Sigma Aldrich (Deisenhofen, Germany). .. Six different N-Ras constructs with different lipid anchors and nucleotide loading (Far/Far (GDP), HD/Far (GDP), HD/HD (GDP), Stbut/Far (GDP), HD/Far (GppNHp, 5′-guanosyl-( β , γ -imido)-triphosphate)), and nonlipidated N-Ras (GDP)) were synthesized as previously described ( ).

    Variant Assay:

    Article Title: Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity
    Article Snippet: D512K and K245G-R252N bicomponent octameric variant of PA was produced as described in ref. [ ]. .. 1,2-Diphytanoyl- sn -glycero-3-phosphocholine (DPhPC), 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS), 1, 2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), bis (monooleoylglycero)phosphate (S,R isomer) (DOBMP), 1,2-diphytanoyl- sn -glycero-3-phosphoethanolamine (DPhPE), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), and bis(monomyristoylglycero)phosphate (S,R isomer) (DMBMP) lipids were purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA (all in chloroform).

    Sequencing:

    Article Title: Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells
    Article Snippet: All of the lipid materials used, 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-cholesterol), 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE), 1,2-distearoyl-sn -glycero-3-phosphoethanolamine-N-[carboxy (polyethylene glycol)-2000] [DSPE-PEG(2000) carboxy], and 1,2-distearoyl-sn -glycero-3-phosphoethanolamine- N-[maleimide (polyethylene glycol)-2000] [DSPE-PEG(2000) maleimide] were obtained from Avanti Polar Lipids (Alabaster, AL). .. The RGD peptide (sequence: H-Gly-Arg-Gly-Asp-Ser-Pro-Lys-Cys-OH, molecular weight, 818.9 Da) was purchased from Yao-Hong Biotechnology Inc (Taipei, Taiwan).

    Sonication:

    Article Title: Improvement of Immunogenicity of Meningococcal Lipooligosaccharide by Coformulation with Lipidated Transferrin-Binding Protein B in Liposomes: Implications for Vaccine Development
    Article Snippet: Small cationic liposomes were prepared by the Bangham method ( ) from ethyl-DOPC and 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) (Avanti Polar Lipids, Inc., Alabaster, AL) in a 3:2 molar ratio, and LOS was incorporated using a detergent-removal method. .. Briefly, 0.3 mol of ethyl-DOPC and 0.2 mol of DOPE in chloroform were evaporated to complete dryness, resuspended in 30 ml of a 10 mM Tris buffer, pH 7.0, and bath sonicated until the formation of a homogeneous suspension of small unilamellar liposomes.

    Recombinant:

    Article Title: Protein S stimulates inhibition of the tissue factor pathway by tissue factor pathway inhibitor
    Article Snippet: Recombinant TF (thromboplastin) was from Dade Innovin (Marburg, Germany). .. 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were obtained from Avanti Polar Lipids.

    Article Title: Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity
    Article Snippet: Recombinant Bacillus anthracis PA83 and LFN were produced as described in refs. [ , ]. .. 1,2-Diphytanoyl- sn -glycero-3-phosphocholine (DPhPC), 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS), 1, 2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), bis (monooleoylglycero)phosphate (S,R isomer) (DOBMP), 1,2-diphytanoyl- sn -glycero-3-phosphoethanolamine (DPhPE), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), and bis(monomyristoylglycero)phosphate (S,R isomer) (DMBMP) lipids were purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA (all in chloroform).

    Chromatin Immunoprecipitation:

    Article Title: Influence of the Lipid Anchor Motif of N-Ras on the Interaction with Lipid Membranes: A Surface Plasmon Resonance Study
    Article Snippet: Lipids (1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) (100%), 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) (100%), 1,2-dioleoyl- sn -glycero-3-(phospho-rac-(1-glycerol)) (sodium salt) (DOPG) (100%), and 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) (100%)) were purchased from Avanti Polar Lipids (Alabaster, AL), and cholesterol (Chol) (≥99%) was obtained from Sigma Aldrich (Deisenhofen, Germany). .. The extruder and suitable polycarbonate membranes (0.1 μ M) and filter supports were purchased from Avanti Polar Lipids, and larger membrane filters (0.2 μ M), as well as the pioneer L1 sensor chip, were obtained from GE Healthcare (Munich, Germany).

    Molecular Weight:

    Article Title: Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells
    Article Snippet: All of the lipid materials used, 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-cholesterol), 1,2-dioleoyl-sn -glycero-3-phosphoethanolamine (DOPE), 1,2-distearoyl-sn -glycero-3-phosphoethanolamine-N-[carboxy (polyethylene glycol)-2000] [DSPE-PEG(2000) carboxy], and 1,2-distearoyl-sn -glycero-3-phosphoethanolamine- N-[maleimide (polyethylene glycol)-2000] [DSPE-PEG(2000) maleimide] were obtained from Avanti Polar Lipids (Alabaster, AL). .. The RGD peptide (sequence: H-Gly-Arg-Gly-Asp-Ser-Pro-Lys-Cys-OH, molecular weight, 818.9 Da) was purchased from Yao-Hong Biotechnology Inc (Taipei, Taiwan).

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    Avanti Polar nbd dope
    Normalization with various detergents. ( A ) Fluorescence after addition of the same volume of buffer or detergent solution (final concentration is 0.83% wt/wt for DDM and 0.67% vol/vol for Triton) to a liposome solution with 18 mM lipids. To ensure a complete dissociation of the liposomes and the absence of FRET within micelles, detergent was added up to 55 mM for Triton (more than 200 times its CMC) and up to 80 mM for DDM (more than 500 times its CMC), without any change in the outcome. Only fluorescent lipids concentrations below 0.01% have been tested because they provide fluorescence intensities close to that of infinite dilution (see Fig. 1 ). Fluorescence is decreased upon addition of detergent. The values are normalized by the average fluorescence after addition of buffer (crosses). All standard deviations are within the size of the markers. ( B ) Relative fluorescence intensities after addition of DDM, Triton or β-OG (final concentration 0.83% wt/wt, 0.67% vol/vol and 0.83% wt/wt respectively) to a liposome solution with 1 mM lipids and containing 1.5% <t>NBD-DOPE</t> and 1.5% Rho-DOPE. The values are normalized by the average fluorescence produced by DDM in panel A. Error bars are standard deviations on more than 50 wells. In both panels, the temperature is set at 37 °C.
    Nbd Dope, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Avanti Polar 1 2 dioleoyl sn glycero 3 phosphoethanolamine dope
    Chemical structures of the lipids used in the study. (A) <t>1,2-diphytanoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DPhPC) and 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS). (B) <t>1,2-dioleoyl-</t> sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoetha-nolamine (DOPE), and bis(monooleoylglycero)phosphate (S,R isomer) (DOBMP).
    1 2 Dioleoyl Sn Glycero 3 Phosphoethanolamine Dope, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dioleoyl sn glycero 3 phosphoethanolamine dope/product/Avanti Polar
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
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    87
    Avanti Polar 1 2 dioleyl sn glycero 3 phosphoethanolamine dope
    Description of liposomal formulations used in the study (A) Illustrations of liposomes used. PL= phospholipids, PEG = polyethylene glycol, <t>DOPE</t> = dioleoylphosphatidyl-ethanolamine, DSPC = <t>distearoyl-sn-glycero-3-phosphocholine,</t> DSPE = <t>1,2-distearoylphosphatidyl-ethanolamine.</t> (B) Liposomal stability in solution. DOX-containing liposomes (1 mM) were incubated in stock solution (5% (w/v) sucrose solution pH 5.0 or in growth medium (DMEM with 10% (v/v) FCS, pH 7.4 for 24, 48 or 72 h. The liposomes were filtered and DOX was quantified to determine the percent of DOX retained in liposomes. The values show average of three experiments with standard deviations. (C) Effect of ceramide on doxorubicin release from liposomes. HeLa cells were incubated with various concentrations (30 – 1000 nM) of four different DOX-loaded liposomes and Free-DOX. After 24 h the cell medium was removed, cells washed and lysed using 0.1% (v/v) Triton-X100. DOX still encapsulated in liposomes was separated from free/released DOX by solid phase extraction and quantified. The data show the percent of DOX being encapsulated in liposomes or free. Mean values obtained by analyzing 3 replicates are shown.
    1 2 Dioleyl Sn Glycero 3 Phosphoethanolamine Dope, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dioleyl sn glycero 3 phosphoethanolamine dope/product/Avanti Polar
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    1 2 dioleyl sn glycero 3 phosphoethanolamine dope - by Bioz Stars, 2020-02
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    Normalization with various detergents. ( A ) Fluorescence after addition of the same volume of buffer or detergent solution (final concentration is 0.83% wt/wt for DDM and 0.67% vol/vol for Triton) to a liposome solution with 18 mM lipids. To ensure a complete dissociation of the liposomes and the absence of FRET within micelles, detergent was added up to 55 mM for Triton (more than 200 times its CMC) and up to 80 mM for DDM (more than 500 times its CMC), without any change in the outcome. Only fluorescent lipids concentrations below 0.01% have been tested because they provide fluorescence intensities close to that of infinite dilution (see Fig. 1 ). Fluorescence is decreased upon addition of detergent. The values are normalized by the average fluorescence after addition of buffer (crosses). All standard deviations are within the size of the markers. ( B ) Relative fluorescence intensities after addition of DDM, Triton or β-OG (final concentration 0.83% wt/wt, 0.67% vol/vol and 0.83% wt/wt respectively) to a liposome solution with 1 mM lipids and containing 1.5% NBD-DOPE and 1.5% Rho-DOPE. The values are normalized by the average fluorescence produced by DDM in panel A. Error bars are standard deviations on more than 50 wells. In both panels, the temperature is set at 37 °C.

    Journal: Scientific Reports

    Article Title: Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes

    doi: 10.1038/srep43860

    Figure Lengend Snippet: Normalization with various detergents. ( A ) Fluorescence after addition of the same volume of buffer or detergent solution (final concentration is 0.83% wt/wt for DDM and 0.67% vol/vol for Triton) to a liposome solution with 18 mM lipids. To ensure a complete dissociation of the liposomes and the absence of FRET within micelles, detergent was added up to 55 mM for Triton (more than 200 times its CMC) and up to 80 mM for DDM (more than 500 times its CMC), without any change in the outcome. Only fluorescent lipids concentrations below 0.01% have been tested because they provide fluorescence intensities close to that of infinite dilution (see Fig. 1 ). Fluorescence is decreased upon addition of detergent. The values are normalized by the average fluorescence after addition of buffer (crosses). All standard deviations are within the size of the markers. ( B ) Relative fluorescence intensities after addition of DDM, Triton or β-OG (final concentration 0.83% wt/wt, 0.67% vol/vol and 0.83% wt/wt respectively) to a liposome solution with 1 mM lipids and containing 1.5% NBD-DOPE and 1.5% Rho-DOPE. The values are normalized by the average fluorescence produced by DDM in panel A. Error bars are standard deviations on more than 50 wells. In both panels, the temperature is set at 37 °C.

    Article Snippet: Material POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and Rho-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) were purchased from Avanti Polar Lipids. β -OG (Octyl β-D-glucopyranoside) and DDM (n-Dodecyl β-D-maltoside) were purchased from Sigma-Aldrich in powder.

    Techniques: Fluorescence, Concentration Assay, Produced

    Infinite dilution of the fluorescent lipids. Intrinsic fluorescence of NBD-DOPE, i.e. total fluorescence divided by the quantity of dyes, was measured for various fractions of fluorescent lipids (NBD-DOPE and Rho-DOPE being both equal). The circles are data points with only fluorescent liposomes at 18 mM lipids. Triangles were obtained from various experiments in which there was a mixture of fluorescent and non-fluorescent liposomes at ratios varying from 1:2 to 1:10; the total lipid concentration also being 18 mM. As fluorescent lipid fractions decrease, FRET between NBD and Rhodamine is reduced. The plateau, corresponding to the absence of FRET, i.e. infinite dilution, is reached for fluorescent lipids fraction bellow or equal to 0.01%, i.e. for ~1 fluorescent lipid per liposome or less. 1 was set as the average of the data points below 0.01% from the purely fluorescent lipids. Error bars are the minimum and maximum values from 4 different wells (often smaller than the marker). The temperature is set at 37 °C.

    Journal: Scientific Reports

    Article Title: Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes

    doi: 10.1038/srep43860

    Figure Lengend Snippet: Infinite dilution of the fluorescent lipids. Intrinsic fluorescence of NBD-DOPE, i.e. total fluorescence divided by the quantity of dyes, was measured for various fractions of fluorescent lipids (NBD-DOPE and Rho-DOPE being both equal). The circles are data points with only fluorescent liposomes at 18 mM lipids. Triangles were obtained from various experiments in which there was a mixture of fluorescent and non-fluorescent liposomes at ratios varying from 1:2 to 1:10; the total lipid concentration also being 18 mM. As fluorescent lipid fractions decrease, FRET between NBD and Rhodamine is reduced. The plateau, corresponding to the absence of FRET, i.e. infinite dilution, is reached for fluorescent lipids fraction bellow or equal to 0.01%, i.e. for ~1 fluorescent lipid per liposome or less. 1 was set as the average of the data points below 0.01% from the purely fluorescent lipids. Error bars are the minimum and maximum values from 4 different wells (often smaller than the marker). The temperature is set at 37 °C.

    Article Snippet: Material POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and Rho-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) were purchased from Avanti Polar Lipids. β -OG (Octyl β-D-glucopyranoside) and DDM (n-Dodecyl β-D-maltoside) were purchased from Sigma-Aldrich in powder.

    Techniques: Fluorescence, Concentration Assay, Marker

    Detergent normalization with the temperature. Following the same procedure as that described in Fig. 2A for liposomes containing 0.001% NBD-DOPE and Rho-DOPE and a concentration of 18 mM lipids. This is done for a range of temperatures between 27 °C and 47 °C.

    Journal: Scientific Reports

    Article Title: Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes

    doi: 10.1038/srep43860

    Figure Lengend Snippet: Detergent normalization with the temperature. Following the same procedure as that described in Fig. 2A for liposomes containing 0.001% NBD-DOPE and Rho-DOPE and a concentration of 18 mM lipids. This is done for a range of temperatures between 27 °C and 47 °C.

    Article Snippet: Material POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and Rho-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) were purchased from Avanti Polar Lipids. β -OG (Octyl β-D-glucopyranoside) and DDM (n-Dodecyl β-D-maltoside) were purchased from Sigma-Aldrich in powder.

    Techniques: Concentration Assay

    Increase of fluorescence upon dequenching after dilution of the dyes. Dequenching obtained from Eq. (2) using solutions containing liposomes with 1.5% (no dilution, d = 1), 0.75% (diluted twice, d = 2) and 0.19% (diluted 8 times, d = 8) NBD-DOPE and Rho-DOPE lipids. Dilution experiments were performed at 32 °C, 37 °C and 42 °C. The results do not depend on the temperature and are consistent with previous reports at 37 °C 12 (crosses). The continuous line, represented between d = 1 and d = 100 corresponds to the fit obtained by using Eq. (3) between d = 1 and d = 8. Representing it until d = 100 was only done for the sake of clarity, to check that, indeed, this fit allowed to find that, from one point, further dilution does not lead to further dequenching, since the dyes are already sufficiently far from each other for FRET not to occur.

    Journal: Scientific Reports

    Article Title: Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes

    doi: 10.1038/srep43860

    Figure Lengend Snippet: Increase of fluorescence upon dequenching after dilution of the dyes. Dequenching obtained from Eq. (2) using solutions containing liposomes with 1.5% (no dilution, d = 1), 0.75% (diluted twice, d = 2) and 0.19% (diluted 8 times, d = 8) NBD-DOPE and Rho-DOPE lipids. Dilution experiments were performed at 32 °C, 37 °C and 42 °C. The results do not depend on the temperature and are consistent with previous reports at 37 °C 12 (crosses). The continuous line, represented between d = 1 and d = 100 corresponds to the fit obtained by using Eq. (3) between d = 1 and d = 8. Representing it until d = 100 was only done for the sake of clarity, to check that, indeed, this fit allowed to find that, from one point, further dilution does not lead to further dequenching, since the dyes are already sufficiently far from each other for FRET not to occur.

    Article Snippet: Material POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), NBD-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) and Rho-DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) were purchased from Avanti Polar Lipids. β -OG (Octyl β-D-glucopyranoside) and DDM (n-Dodecyl β-D-maltoside) were purchased from Sigma-Aldrich in powder.

    Techniques: Fluorescence

    Chemical structures of the lipids used in the study. (A) 1,2-diphytanoyl- sn -glycero-3-phosphocholine (DPhPC) and 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS). (B) 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoetha-nolamine (DOPE), and bis(monooleoylglycero)phosphate (S,R isomer) (DOBMP).

    Journal: Biochimica et biophysica acta. Biomembranes

    Article Title: Effect of late endosomal DOBMP lipid and traditional model lipids of electrophysiology on the anthrax toxin channel activity

    doi: 10.1016/j.bbamem.2018.08.011

    Figure Lengend Snippet: Chemical structures of the lipids used in the study. (A) 1,2-diphytanoyl- sn -glycero-3-phosphocholine (DPhPC) and 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS). (B) 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoetha-nolamine (DOPE), and bis(monooleoylglycero)phosphate (S,R isomer) (DOBMP).

    Article Snippet: 1,2-Diphytanoyl- sn -glycero-3-phosphocholine (DPhPC), 1,2-diphytanoyl- sn -glycero-3-phospho-L-serine (DPhPS), 1, 2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), 1,2-dioleoyl- sn -glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), bis (monooleoylglycero)phosphate (S,R isomer) (DOBMP), 1,2-diphytanoyl- sn -glycero-3-phosphoethanolamine (DPhPE), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), and bis(monomyristoylglycero)phosphate (S,R isomer) (DMBMP) lipids were purchased from Avanti Polar Lipids, Inc., Alabaster, AL, USA (all in chloroform).

    Techniques:

    Schematic drawing of lipoplex assembly and radiolabeling procedures of the gene delivery nanosystem. Abbreviation: DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine.

    Journal: International Journal of Nanomedicine

    Article Title: A 89Zr-labeled lipoplex nanosystem for image-guided gene delivery: design, evaluation of stability and in vivo behavior

    doi: 10.2147/IJN.S179806

    Figure Lengend Snippet: Schematic drawing of lipoplex assembly and radiolabeling procedures of the gene delivery nanosystem. Abbreviation: DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine.

    Article Snippet: 3,3′-Iminobis( N , N -dimethylpropylamine), Boc-Gly-OH, Fmoc-Lys(Boc)-OH, and 1-Iodododecane were purchased from Sigma-Aldrich Co. (St Louis, MO, USA); N , N ′- Diisopropylethylamine (DIPEA) and O-(7-Azabenzotriazol-1-yl)- N , N , N ′, N ′-tetramethyluronium hexafluorophosphate (HATU) were purchased from Chem-Impex International, Inc (Wood Dale, IL, USA); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was purchased from Avanti Polar Lipids, Inc (Alabaster, AL, USA) and p-SCN-Bn-Deferoxamine (deferoxamine) was purchased from Macrocyclics, Inc (Cat. No: B-705) (Dallas, TX, USA).

    Techniques: Radioactivity

    Description of liposomal formulations used in the study (A) Illustrations of liposomes used. PL= phospholipids, PEG = polyethylene glycol, DOPE = dioleoylphosphatidyl-ethanolamine, DSPC = distearoyl-sn-glycero-3-phosphocholine, DSPE = 1,2-distearoylphosphatidyl-ethanolamine. (B) Liposomal stability in solution. DOX-containing liposomes (1 mM) were incubated in stock solution (5% (w/v) sucrose solution pH 5.0 or in growth medium (DMEM with 10% (v/v) FCS, pH 7.4 for 24, 48 or 72 h. The liposomes were filtered and DOX was quantified to determine the percent of DOX retained in liposomes. The values show average of three experiments with standard deviations. (C) Effect of ceramide on doxorubicin release from liposomes. HeLa cells were incubated with various concentrations (30 – 1000 nM) of four different DOX-loaded liposomes and Free-DOX. After 24 h the cell medium was removed, cells washed and lysed using 0.1% (v/v) Triton-X100. DOX still encapsulated in liposomes was separated from free/released DOX by solid phase extraction and quantified. The data show the percent of DOX being encapsulated in liposomes or free. Mean values obtained by analyzing 3 replicates are shown.

    Journal: Oncotarget

    Article Title: Ceramide-containing liposomes with doxorubicin: time and cell-dependent effect of C6 and C12 ceramide

    doi: 10.18632/oncotarget.20217

    Figure Lengend Snippet: Description of liposomal formulations used in the study (A) Illustrations of liposomes used. PL= phospholipids, PEG = polyethylene glycol, DOPE = dioleoylphosphatidyl-ethanolamine, DSPC = distearoyl-sn-glycero-3-phosphocholine, DSPE = 1,2-distearoylphosphatidyl-ethanolamine. (B) Liposomal stability in solution. DOX-containing liposomes (1 mM) were incubated in stock solution (5% (w/v) sucrose solution pH 5.0 or in growth medium (DMEM with 10% (v/v) FCS, pH 7.4 for 24, 48 or 72 h. The liposomes were filtered and DOX was quantified to determine the percent of DOX retained in liposomes. The values show average of three experiments with standard deviations. (C) Effect of ceramide on doxorubicin release from liposomes. HeLa cells were incubated with various concentrations (30 – 1000 nM) of four different DOX-loaded liposomes and Free-DOX. After 24 h the cell medium was removed, cells washed and lysed using 0.1% (v/v) Triton-X100. DOX still encapsulated in liposomes was separated from free/released DOX by solid phase extraction and quantified. The data show the percent of DOX being encapsulated in liposomes or free. Mean values obtained by analyzing 3 replicates are shown.

    Article Snippet: All lipids; 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] ammonium salt (DSPE-PEG2000), 1,2-dioleyl-sn-glycero-3-phosphoethanolamine (DOPE), N-hexanoyl-D-erythro-sphingosine (Ceramide C6), and N-lauroyl-D-erythro-sphingosine (Ceramide C12), were purchased from Avanti Polar Lipids (Alabaster, AL, USA).

    Techniques: Incubation