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Murashige and Skoog plant salt mixture w o agar

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Murashige and Skoog Medium contains Murashige and Skoog salts as well as a mixture of vitamins This modification of the original formula does not contain agar Murashige and Skoog Medium is widely used plant tissue culture growth medium It has been proven effective in the culture of tissue derived from moncotyledons and dicotyledons This medium originally formulated to support tobacco callus has been used to support callus initiation and growth the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants More recently it has been used for micropropagation of ornamental vegetable and fruit plants in research and commercial enterprises

Catalog Number:

0926330-cf

Price:

None

Category:

Life Sciences Cell Biology Culture Media Plant Growth Media

Applications:

Plant Cell Growth, Plant tissue culture growth

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Structured Review

Valiant skoog
Murashige and Skoog plant salt mixture w o agar

skoog - by Bioz Stars, 2021-03

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Article Title: Root Architecture Diversity and Meristem Dynamics in Different Populations of Arabidopsis thaliana
Article Snippet: Plant Growth Conditions Seeds of the 12 Arabidopsis accessions used in this study were disinfected in 20% sodium hypochlorite and 0.01% of Tween-20 for 15 min. Then seeds were stratified at 4°C for 5 days under dark conditions and sown on square petri dishes containing 0.2X Murashige and Skoog salts (MP Biomedicals), 0.05% MES (SigmaAldrich), 1% sucrose (SigmaAldrich) and 1% agar (Becton, Dickinson and Company) at pH = 5.6.

Article Title: Light dependent activation of the GCN2 kinase under cold and salt stress is mediated by the photosynthetic status of the chloroplast
Article Snippet: Seeds were germinated on half-strength Murashige-Skoog (1/2X MS) plant media (MP Biomedicals, cat # 2633024) with 0.65% Phytoagar (Bioworld, cat # 40100072-2) and grown under a long-day period of 16 h light (80±10 μEin m−2 s−1)/8 h dark at 22 °C and 50% humidity.

Article Title: ELF18-INDUCED LONG-NONCODING RNA Associates with Mediator to Enhance Expression of Innate Immune Response Genes in Arabidopsis
Article Snippet: Plants of all genotypes were grown on 0.6% agar media containing 0.5× Murashige and Skoog ( ) salts (MP Biomedicals), 1% sucrose (Fisher), and 0.5 g/L MES hydrate (Sigma-Aldrich) in a growth room at 22°C under 16 h light/8 h dark with white fluorescent light (∼100 μmol m−2 s−1 ).

Article Title: Light activates the translational regulatory GCN2 kinase via reactive oxygen species emanating from the chloroplast
Article Snippet: Seeds were germinated on ½-strength Murashige-Skoog (MS) salt plant media (MP Biomedicals, cat # 2633024) with 0.65% Phytoagar (Bioworld, cat # 40100072-2) under a long-day cycle of 16 hr light (80±10 µEin m-2 s-1 )/8 hr dark at 22 °C and 50% humidity.

Article Title: Identification of Arabidopsis GPAT9 (At5g60620) as an Essential Gene Involved in Triacylglycerol Biosynthesis 1 (At5g60620) as an Essential Gene Involved in Triacylglycerol Biosynthesis 1 [OPEN]
Article Snippet: Seeds were surface sterilized (30% [v/v] ethanol, 10% [v/v] bleach, and 0.1% [w/v] SDS) for 5 min, rinsed five times with sterile water, and plated onto germination medium (2.5 m m MES, pH 5.7, 1% [w/v] Suc, 1× Murashige and Skoog Plant Salt Mix [MP Biomedicals; ], and 0.8% [w/v] agar).

Mass Spectrometry:

Article Title: Genome mining of Streptomyces scabrisporus NF3 reveals symbiotic features including genes related to plant interactions
Article Snippet: Hydrolysis zones were detected as cleared zones after 7 and 14 days of incubation. .. Colonization of Arabidopsis thaliana Seeds of Arabidopsis thaliana Col-0 used in this study were disinfected in 20% sodium hypochlorite and 0.01% of Tween-20 for 15 min. Then seeds were stratified at 4°C for 4 days under dark conditions and sown on square petri dishes containing 0.2X Murashige and Skoog salts (MS) (MP Biomedicals), 0.05% MES (SigmaAldrich), 1% sucrose (SigmaAldrich) and 1% agar (Becton, Dickinson and Company) at pH = 5.6. .. At the moment of inoculation in MS plates, one group of seeds were inoculated with 10^7 CFU of bacterial mycelium previously grown in YMG for three days and washed (3x) in phosphate saline buffer (PBS).

Expressing:

Article Title: Rice Cytokinin GATA Transcription Factor1 Regulates Chloroplast Development and Plant Architecture 1Rice Cytokinin GATA Transcription Factor1 Regulates Chloroplast Development and Plant Architecture 1 [W]Rice Cytokinin GATA Transcription Factor1 Regulates Chloroplast Development and Plant Architecture 1 [W] [OA]
Article Snippet: .. In order to determine factors influencing Cga1 expression, wild-type plants were grown hydroponically for 2 weeks in 1× Murashige and Skoog plant salts (MP Biomedicals), removed from nutrients to deionized water in darkness for 24 h, and then resupplemented with (10 m m NO3 − ) or 10 µmol of BA (Fluka), while GA3 (Sigma) was applied as a 10-µmol foliar spray. ..

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