dpbs  (Valiant)


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    Valiant dpbs
    Dpbs, supplied by Valiant, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dpbs/product/Valiant
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dpbs - by Bioz Stars, 2022-09
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    Valiant phosphate buffered saline pbs
    Relative viral replication and recombination in the presence of bacterial protein. Using primers specific to hexon sequence in HAdV-D19 (A and C) and HAdV-D29 (B and D), quantitative PCR was used to quantify total viral DNA from 1 to 8 days postinfection in <t>PBS-treated</t> versus E . coli K-12 lysate-treated C2BBe1 cells (A and B) and A549 cells (C and D). DNA quantity is graphed relative to the levels at 1 day postinfection. (E and F) C2BBe1 (E) and A549 (F) cells pretreated with <t>PBS,</t> K-12 lysate, DH5α lysate, or K-12 lysate depleted of RecA were coinfected with HAdV-D19 and HAdV-D29 and subjected to quantitative PCR at 5 to 8 days postinfection, with primers chosen to amplify only the HVL2 recombinant. Values that are significantly different ( P
    Phosphate Buffered Saline Pbs, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs - by Bioz Stars, 2022-09
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    Relative viral replication and recombination in the presence of bacterial protein. Using primers specific to hexon sequence in HAdV-D19 (A and C) and HAdV-D29 (B and D), quantitative PCR was used to quantify total viral DNA from 1 to 8 days postinfection in PBS-treated versus E . coli K-12 lysate-treated C2BBe1 cells (A and B) and A549 cells (C and D). DNA quantity is graphed relative to the levels at 1 day postinfection. (E and F) C2BBe1 (E) and A549 (F) cells pretreated with PBS, K-12 lysate, DH5α lysate, or K-12 lysate depleted of RecA were coinfected with HAdV-D19 and HAdV-D29 and subjected to quantitative PCR at 5 to 8 days postinfection, with primers chosen to amplify only the HVL2 recombinant. Values that are significantly different ( P

    Journal: mSphere

    Article Title: Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection

    doi: 10.1128/mSphere.00105-18

    Figure Lengend Snippet: Relative viral replication and recombination in the presence of bacterial protein. Using primers specific to hexon sequence in HAdV-D19 (A and C) and HAdV-D29 (B and D), quantitative PCR was used to quantify total viral DNA from 1 to 8 days postinfection in PBS-treated versus E . coli K-12 lysate-treated C2BBe1 cells (A and B) and A549 cells (C and D). DNA quantity is graphed relative to the levels at 1 day postinfection. (E and F) C2BBe1 (E) and A549 (F) cells pretreated with PBS, K-12 lysate, DH5α lysate, or K-12 lysate depleted of RecA were coinfected with HAdV-D19 and HAdV-D29 and subjected to quantitative PCR at 5 to 8 days postinfection, with primers chosen to amplify only the HVL2 recombinant. Values that are significantly different ( P

    Article Snippet: E . coli K-12 and DH5α were each inoculated into Luria broth (LB), incubated at 37°C for 16 h in a shaking incubator at 225 rpm, then collected by centrifugation in 50-ml conical tubes at 5,000 rpm for 10 min, and resuspended in 10 ml of phosphate-buffered saline (PBS), and aliquots of the bacterial solutions were added to Lysing Matrix B tubes (MP Biomedicals, Santa Ana, CA).

    Techniques: Sequencing, Real-time Polymerase Chain Reaction, Recombinant

    BoxA inhibits the thinning of retinal layers determined by OCT. ( a ) Representative image of the control, TON + PBS, and TON+ BoxA. Two vertical calipers were placed on each side of the optic nerve head, 800 and 1000 µm away from the center of the optic nerve head. The combined thickness (µm) of the NFL, GCL, and IPL was measured. ( b ) Averaged data of retinal thickness from recordings. ( c ) The inner retina thickness was normalized to those of the control eyes, expressed as a percentage, and plotted as a function of the post-ONC time course. Each piece of data represents the mean ± SD, n = 6. *** p

    Journal: International Journal of Molecular Sciences

    Article Title: High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy

    doi: 10.3390/ijms23126715

    Figure Lengend Snippet: BoxA inhibits the thinning of retinal layers determined by OCT. ( a ) Representative image of the control, TON + PBS, and TON+ BoxA. Two vertical calipers were placed on each side of the optic nerve head, 800 and 1000 µm away from the center of the optic nerve head. The combined thickness (µm) of the NFL, GCL, and IPL was measured. ( b ) Averaged data of retinal thickness from recordings. ( c ) The inner retina thickness was normalized to those of the control eyes, expressed as a percentage, and plotted as a function of the post-ONC time course. Each piece of data represents the mean ± SD, n = 6. *** p

    Article Snippet: Isolated retinas were washed twice with phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (04807423, MP Biomedicals, Santa Ana, California, USA) and simultaneously were gently shaken for 15 min, blotted with PBS containing 3% BSA (FA016, GENVIEW, Beijing, China) plus 0.1% Triton X-100 at room temperature for 2 h, then incubated with primary antibody targeting the RGC-specific marker RNA Binding Protein with multiple splicing (RBPMS, ABN1362, Millipore, USA) overnight at 4 °C.

    Techniques:

    The effects of BoxA on the activation of NLRP3, ASC, and NF-kB. ( a ) The protein level of NLRP3, ASC, and NF-kB in the retina from the control normal group, ONC + PBS group, and ONC+ BoxA group was examined by simple western immunoblots. GAPDH was used to ensure equal loading. ( b ) Analysis of the effects of BoxA on protein expression. Each piece of data represents the mean ± SD, n = 3. * p

    Journal: International Journal of Molecular Sciences

    Article Title: High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy

    doi: 10.3390/ijms23126715

    Figure Lengend Snippet: The effects of BoxA on the activation of NLRP3, ASC, and NF-kB. ( a ) The protein level of NLRP3, ASC, and NF-kB in the retina from the control normal group, ONC + PBS group, and ONC+ BoxA group was examined by simple western immunoblots. GAPDH was used to ensure equal loading. ( b ) Analysis of the effects of BoxA on protein expression. Each piece of data represents the mean ± SD, n = 3. * p

    Article Snippet: Isolated retinas were washed twice with phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (04807423, MP Biomedicals, Santa Ana, California, USA) and simultaneously were gently shaken for 15 min, blotted with PBS containing 3% BSA (FA016, GENVIEW, Beijing, China) plus 0.1% Triton X-100 at room temperature for 2 h, then incubated with primary antibody targeting the RGC-specific marker RNA Binding Protein with multiple splicing (RBPMS, ABN1362, Millipore, USA) overnight at 4 °C.

    Techniques: Activation Assay, Western Blot, Expressing

    BOXA prevents RGCs function. ( a ) Representative Positive Scotopic Threshold Responses (pSTRs) of the control (black line), TON + PBS group (grey line), and TON + BoxA group (brown line) 7 days post ONC. ( b ) Averaged data of the pSTR amplitudes from recordings. ( c ) Representative Pattern Electroretinogram (PERG) of the control (black line), TON + PBS group (grey line), and TON + BoxA group (brown line) 6 days post ONC. ( d ) Averaged data of the N2 wave amplitudes from recordings. ( e , f ) The pSTR and N2 wave amplitudes were normalized to those of the control eyes, expressed as a percentage, and plotted as a function of the post-ONC time course. Each piece of data represents the mean ± SD, n = 6. ** p

    Journal: International Journal of Molecular Sciences

    Article Title: High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy

    doi: 10.3390/ijms23126715

    Figure Lengend Snippet: BOXA prevents RGCs function. ( a ) Representative Positive Scotopic Threshold Responses (pSTRs) of the control (black line), TON + PBS group (grey line), and TON + BoxA group (brown line) 7 days post ONC. ( b ) Averaged data of the pSTR amplitudes from recordings. ( c ) Representative Pattern Electroretinogram (PERG) of the control (black line), TON + PBS group (grey line), and TON + BoxA group (brown line) 6 days post ONC. ( d ) Averaged data of the N2 wave amplitudes from recordings. ( e , f ) The pSTR and N2 wave amplitudes were normalized to those of the control eyes, expressed as a percentage, and plotted as a function of the post-ONC time course. Each piece of data represents the mean ± SD, n = 6. ** p

    Article Snippet: Isolated retinas were washed twice with phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (04807423, MP Biomedicals, Santa Ana, California, USA) and simultaneously were gently shaken for 15 min, blotted with PBS containing 3% BSA (FA016, GENVIEW, Beijing, China) plus 0.1% Triton X-100 at room temperature for 2 h, then incubated with primary antibody targeting the RGC-specific marker RNA Binding Protein with multiple splicing (RBPMS, ABN1362, Millipore, USA) overnight at 4 °C.

    Techniques:

    Intracellular metal content of WT and isogenic mutant GAS strains as assessed by ICP-MS. GAS strains were grown to the mid-exponential growth phase (A 600 , 0.8) in THY broth supplemented with TPEN. (A) The intracellular Zn levels of WT, Δ adcC , and Δ rpsN.2 strains grown in THY supplemented with 30 μM TPEN are shown. (B) The cytosolic Zn levels of each indicated strain grown in THY supplemented with 32.5 μM TPEN are shown. Cell pellets were washed twice in PBS containing 1 mM nitrilotriacetic acid, followed by two washes in chelexed PBS, and suspended in chelexed PBS. The Zn content in the clarified cell lysate was analyzed with ICP-MS. The Zn levels were normalized to the total cytosolic protein concentration. The data are the mean ± standard deviation for two biological replicates. P values (*, P

    Journal: Infection and Immunity

    Article Title: Group A Streptococcus AdcR Regulon Participates in Bacterial Defense against Host-Mediated Zinc Sequestration and Contributes to Virulence

    doi: 10.1128/IAI.00097-20

    Figure Lengend Snippet: Intracellular metal content of WT and isogenic mutant GAS strains as assessed by ICP-MS. GAS strains were grown to the mid-exponential growth phase (A 600 , 0.8) in THY broth supplemented with TPEN. (A) The intracellular Zn levels of WT, Δ adcC , and Δ rpsN.2 strains grown in THY supplemented with 30 μM TPEN are shown. (B) The cytosolic Zn levels of each indicated strain grown in THY supplemented with 32.5 μM TPEN are shown. Cell pellets were washed twice in PBS containing 1 mM nitrilotriacetic acid, followed by two washes in chelexed PBS, and suspended in chelexed PBS. The Zn content in the clarified cell lysate was analyzed with ICP-MS. The Zn levels were normalized to the total cytosolic protein concentration. The data are the mean ± standard deviation for two biological replicates. P values (*, P

    Article Snippet: Cells were subsequently washed in sterile chelexed PBS, suspended in 0.4 ml sterile PBS, and lysed by ballistic disintegration (lysing matrix B and Fastprep96 automated homogenizer; MP Biomedicals).

    Techniques: Mutagenesis, Protein Concentration, Standard Deviation

    The anti-bacillus effect of vitamin B5 (VB5) in mice with Mycobacterium tuberculosis infection. C57BL/6J mice were infected with H37Rv (~200 bacteria/mouse). Oral administration with phosphate buffer solution, VB5, and INH was started from the day after infection (day 1) and continued for 1, 2, and 4 weeks alternatively. The lungs and spleens were analyzed at indicated time. (A) Colony-forming units (CFUs) were obtained from the lungs and spleen cell lysates by serial dilution and plating on 7H10 agar in triplicate. The colonies were counted after 4 weeks. (B) Spleen weights were detected. Data shown are the mean ± SD. * P

    Journal: Frontiers in Immunology

    Article Title: Vitamin B5 Reduces Bacterial Growth via Regulating Innate Immunity and Adaptive Immunity in Mice Infected with Mycobacterium tuberculosis

    doi: 10.3389/fimmu.2018.00365

    Figure Lengend Snippet: The anti-bacillus effect of vitamin B5 (VB5) in mice with Mycobacterium tuberculosis infection. C57BL/6J mice were infected with H37Rv (~200 bacteria/mouse). Oral administration with phosphate buffer solution, VB5, and INH was started from the day after infection (day 1) and continued for 1, 2, and 4 weeks alternatively. The lungs and spleens were analyzed at indicated time. (A) Colony-forming units (CFUs) were obtained from the lungs and spleen cell lysates by serial dilution and plating on 7H10 agar in triplicate. The colonies were counted after 4 weeks. (B) Spleen weights were detected. Data shown are the mean ± SD. * P

    Article Snippet: Followed, VB5 (MP Biomedicals, USA), isoniazid (INH), and phosphate buffer solution (PBS) were alternatively administered orally.

    Techniques: Mouse Assay, Infection, Serial Dilution

    Vitamin B5 (VB5) promoted the Mycobacterium tuberculosis (MTB) phagocytosis by macrophages and the clearance of intracellular mycobacteria in macrophages. (A) Bone marrow-derived macrophages (BMDMs) were pretreated with VB5 followed by MTB H37Rv infection, and intracellular viable bacteria were detected with colony-forming unit (CFU) assays at 0, 1, 2, and 3 day postinfection. (B,C) BMDMs were pretreated with phosphate buffer solution or VB5 (10 µM) for 24 h and then challenged with Texas-Red-labeled MTB H37Rv (multiplicity of infection = 5) for 1 h. (B) Phagocytosis of MTB H37Rv was determined by flow cytometry. (C) Mean fluorescence intensity (MFI) was calculated. (D) Survival rate was calculated via the method that the numbers of viable bacillus at different times divided the number of viable bacillus at 0 day. Data shown are the mean ± SD. * P

    Journal: Frontiers in Immunology

    Article Title: Vitamin B5 Reduces Bacterial Growth via Regulating Innate Immunity and Adaptive Immunity in Mice Infected with Mycobacterium tuberculosis

    doi: 10.3389/fimmu.2018.00365

    Figure Lengend Snippet: Vitamin B5 (VB5) promoted the Mycobacterium tuberculosis (MTB) phagocytosis by macrophages and the clearance of intracellular mycobacteria in macrophages. (A) Bone marrow-derived macrophages (BMDMs) were pretreated with VB5 followed by MTB H37Rv infection, and intracellular viable bacteria were detected with colony-forming unit (CFU) assays at 0, 1, 2, and 3 day postinfection. (B,C) BMDMs were pretreated with phosphate buffer solution or VB5 (10 µM) for 24 h and then challenged with Texas-Red-labeled MTB H37Rv (multiplicity of infection = 5) for 1 h. (B) Phagocytosis of MTB H37Rv was determined by flow cytometry. (C) Mean fluorescence intensity (MFI) was calculated. (D) Survival rate was calculated via the method that the numbers of viable bacillus at different times divided the number of viable bacillus at 0 day. Data shown are the mean ± SD. * P

    Article Snippet: Followed, VB5 (MP Biomedicals, USA), isoniazid (INH), and phosphate buffer solution (PBS) were alternatively administered orally.

    Techniques: Derivative Assay, Infection, Labeling, Flow Cytometry, Fluorescence