Journal: International Journal of Molecular Sciences
Article Title: High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy
Figure Lengend Snippet: The effects of BoxA on the activation of NLRP3, ASC, and NF-kB. ( a ) The protein level of NLRP3, ASC, and NF-kB in the retina from the control normal group, ONC + PBS group, and ONC+ BoxA group was examined by simple western immunoblots. GAPDH was used to ensure equal loading. ( b ) Analysis of the effects of BoxA on protein expression. Each piece of data represents the mean ± SD, n = 3. * p
Article Snippet: Isolated retinas were washed twice with phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (04807423, MP Biomedicals, Santa Ana, California, USA) and simultaneously were gently shaken for 15 min, blotted with PBS containing 3% BSA (FA016, GENVIEW, Beijing, China) plus 0.1% Triton X-100 at room temperature for 2 h, then incubated with primary antibody targeting the RGC-specific marker RNA Binding Protein with multiple splicing (RBPMS, ABN1362, Millipore, USA) overnight at 4 °C.
Techniques: Activation Assay, Western Blot, Expressing