water activity  (Valiant)


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  • 94
    Name:
    Water for cell culture
    Description:
    For use in the preparation of cell culture media and cell suspension and washing solutions
    Catalog Number:
    091696054
    Price:
    12.0
    Applications:
    Mammalian Cell Culture, Preparation of cell culture media
    Size:
    500 mL
    Category:
    Life Sciences Cell Biology Culture Media Mammalian Cell Culture Media
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    Structured Review

    Valiant water activity
    Water for cell culture
    For use in the preparation of cell culture media and cell suspension and washing solutions
    https://www.bioz.com/result/water activity/product/Valiant
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    water activity - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    Radioactivity:

    Article Title: High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds 1
    Article Snippet: .. After incubation, the embryos were rinsed three times with distilled water to remove surface radioactivity and then transferred to scintillation vials where they were dissolved with 2 mL of NCS tissue solubilizing fluid (MP Biomedicals) at 45°C until fully dissolved. .. The total counts of radioactivity present in the fresh medium was compared with counts in the spent media, the dissolved embryos, and the KOH traps by transferring aliquots of each sample type to scintillation vials containing high flash point cocktail safety-solve scintillation fluid (National Diagnostics).

    Filtration:

    Article Title: FACS-mediated isolation of neuronal cell populations from virus infected human embryonic stem cell derived cerebral organoid cultures
    Article Snippet: .. VERO cells (ATCC® CCL-81™ ) Vero cell culture medium (see recipe) Vero cell infection medium (see recipe) T175 culture flasks ZIKA virus strain, for example Uganda 1947 (MR 766, ATCC® 1838™ ) or Puerto Rico 2015 (PRVABC59, ATCC® VR-1843™ ) 2 ml cryogenic vials 6-well flat bottom cell culture plates 2% Oxoid agar stock solution (see recipe) 2X Overlay medium (see recipe) 5% Sodium bicarbonate (Fisher Scientific, cat. no. L-14639) w/v in distilled water 1% DEAE Dextran (MP Biomedicals, cat. no. 195133) w/v in distilled water, sterilize by filtration 4% PFA in Phosphate-Buffered Saline (Boston BioProducts, cat. no. BM-155) Dulbecco’s Phosphate-Buffered Saline (PBS) – no calcium or magnesium (Invitrogen, cat. no. 14190144) 1% Crystal violet (Fisher Scientific, cat. no. C581-100) w/v in distilled water Methanol 2D cerebral organoid culture of 7 days (Step 16 of basic protocol 1) Cerebral organoid medium (see recipe) Penicillin/Streptomycin (Sigma, cat. no. P4333) Accutase (Innovative Cell Technologies, cat. no. AT104) DMEM/F12 (Gibco, cat. no. 11330-032) Cell scrapers EDTA solution (Invitrogen, cat. no. 15575-038) Fetal bovine serum (HyClone, cat. no. SH30071.03) PermWash buffer (BD Biosciences, cat. no. 554723) Fluorochrome-conjugated antibodies (see ) .. 1 Grow Vero cells in Vero cell culture medium to ~80% confluence, dissociate with EDTA/trypsin and seed 7.5 million cells in a T175 culture flask.

    Infection:

    Article Title: FACS-mediated isolation of neuronal cell populations from virus infected human embryonic stem cell derived cerebral organoid cultures
    Article Snippet: .. VERO cells (ATCC® CCL-81™ ) Vero cell culture medium (see recipe) Vero cell infection medium (see recipe) T175 culture flasks ZIKA virus strain, for example Uganda 1947 (MR 766, ATCC® 1838™ ) or Puerto Rico 2015 (PRVABC59, ATCC® VR-1843™ ) 2 ml cryogenic vials 6-well flat bottom cell culture plates 2% Oxoid agar stock solution (see recipe) 2X Overlay medium (see recipe) 5% Sodium bicarbonate (Fisher Scientific, cat. no. L-14639) w/v in distilled water 1% DEAE Dextran (MP Biomedicals, cat. no. 195133) w/v in distilled water, sterilize by filtration 4% PFA in Phosphate-Buffered Saline (Boston BioProducts, cat. no. BM-155) Dulbecco’s Phosphate-Buffered Saline (PBS) – no calcium or magnesium (Invitrogen, cat. no. 14190144) 1% Crystal violet (Fisher Scientific, cat. no. C581-100) w/v in distilled water Methanol 2D cerebral organoid culture of 7 days (Step 16 of basic protocol 1) Cerebral organoid medium (see recipe) Penicillin/Streptomycin (Sigma, cat. no. P4333) Accutase (Innovative Cell Technologies, cat. no. AT104) DMEM/F12 (Gibco, cat. no. 11330-032) Cell scrapers EDTA solution (Invitrogen, cat. no. 15575-038) Fetal bovine serum (HyClone, cat. no. SH30071.03) PermWash buffer (BD Biosciences, cat. no. 554723) Fluorochrome-conjugated antibodies (see ) .. 1 Grow Vero cells in Vero cell culture medium to ~80% confluence, dissociate with EDTA/trypsin and seed 7.5 million cells in a T175 culture flask.

    Light Microscopy:

    Article Title: Fibrin Microthreads Support Mesenchymal Stem Cell Growth While Maintaining Differentiation Potential
    Article Snippet: .. At the conclusion of the feeding cycle adipogenic cultures were fixed in 4% paraformaldehyde, rinsed in distilled water, dehydrated in 60% isopropanol, stained with 0.3% Oil Red O (MP Biomedical, Solon, OH) in distilled water, rinsed in 60% isopropanol, counterstained with hematoxylin (Sigma Aldrich, St. Louis, MO), rinsed with distilled water, and viewed under a light microscope. .. For osteogenic differentiation, cells were re-plated at 5 × 103 cells per cm2 tissue culture surface area and cultured overnight in MSCGM.

    Incubation:

    Article Title: High Flux Through the Oxidative Pentose Phosphate Pathway Lowers Efficiency in Developing Camelina Seeds 1
    Article Snippet: .. After incubation, the embryos were rinsed three times with distilled water to remove surface radioactivity and then transferred to scintillation vials where they were dissolved with 2 mL of NCS tissue solubilizing fluid (MP Biomedicals) at 45°C until fully dissolved. .. The total counts of radioactivity present in the fresh medium was compared with counts in the spent media, the dissolved embryos, and the KOH traps by transferring aliquots of each sample type to scintillation vials containing high flash point cocktail safety-solve scintillation fluid (National Diagnostics).

    Activity Assay:

    Article Title: Effect of Brine Concentrations on the Bacteriological and Chemical Quality and Histamine Content of Brined and Dried Milkfish
    Article Snippet: .. Determination of pH Value, Moisture Content, Water Activity, and Salt ContentTen grams of the samples was weighted and homogenized with a mixer (FastPrep-24, MP Biomedicals, Solon, OH, USA) for 2 min with 20 mL of deionized water to make a thick slurry. .. The pH of this slurry was determined using a digital pH meter (Mettler Toledo FE20/EL20, Schwerzenbach, Switzerland).

    Cell Culture:

    Article Title: FACS-mediated isolation of neuronal cell populations from virus infected human embryonic stem cell derived cerebral organoid cultures
    Article Snippet: .. VERO cells (ATCC® CCL-81™ ) Vero cell culture medium (see recipe) Vero cell infection medium (see recipe) T175 culture flasks ZIKA virus strain, for example Uganda 1947 (MR 766, ATCC® 1838™ ) or Puerto Rico 2015 (PRVABC59, ATCC® VR-1843™ ) 2 ml cryogenic vials 6-well flat bottom cell culture plates 2% Oxoid agar stock solution (see recipe) 2X Overlay medium (see recipe) 5% Sodium bicarbonate (Fisher Scientific, cat. no. L-14639) w/v in distilled water 1% DEAE Dextran (MP Biomedicals, cat. no. 195133) w/v in distilled water, sterilize by filtration 4% PFA in Phosphate-Buffered Saline (Boston BioProducts, cat. no. BM-155) Dulbecco’s Phosphate-Buffered Saline (PBS) – no calcium or magnesium (Invitrogen, cat. no. 14190144) 1% Crystal violet (Fisher Scientific, cat. no. C581-100) w/v in distilled water Methanol 2D cerebral organoid culture of 7 days (Step 16 of basic protocol 1) Cerebral organoid medium (see recipe) Penicillin/Streptomycin (Sigma, cat. no. P4333) Accutase (Innovative Cell Technologies, cat. no. AT104) DMEM/F12 (Gibco, cat. no. 11330-032) Cell scrapers EDTA solution (Invitrogen, cat. no. 15575-038) Fetal bovine serum (HyClone, cat. no. SH30071.03) PermWash buffer (BD Biosciences, cat. no. 554723) Fluorochrome-conjugated antibodies (see ) .. 1 Grow Vero cells in Vero cell culture medium to ~80% confluence, dissociate with EDTA/trypsin and seed 7.5 million cells in a T175 culture flask.

    Staining:

    Article Title: Fibrin Microthreads Support Mesenchymal Stem Cell Growth While Maintaining Differentiation Potential
    Article Snippet: .. At the conclusion of the feeding cycle adipogenic cultures were fixed in 4% paraformaldehyde, rinsed in distilled water, dehydrated in 60% isopropanol, stained with 0.3% Oil Red O (MP Biomedical, Solon, OH) in distilled water, rinsed in 60% isopropanol, counterstained with hematoxylin (Sigma Aldrich, St. Louis, MO), rinsed with distilled water, and viewed under a light microscope. .. For osteogenic differentiation, cells were re-plated at 5 × 103 cells per cm2 tissue culture surface area and cultured overnight in MSCGM.