anti hamster igg whole molecule goat affinity purified antibody  (Valiant)

 
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    Name:
    Anti hamster IgG whole molecule goat affinity purified antibody
    Description:
    Product is goat affinity purified antibody to hamster IgG whole molecule and buffer salts
    Catalog Number:
    0856984
    Price:
    205.8
    Category:
    Life Sciences Antibodies Secondary Antibodies
    Applications:
    Immunoassays, Immunohistochemistry, ELISA, Immunoblot
    Size:
    2 mg
    Buy from Supplier


    Structured Review

    Valiant anti hamster igg whole molecule goat affinity purified antibody
    Anti hamster IgG whole molecule goat affinity purified antibody
    Product is goat affinity purified antibody to hamster IgG whole molecule and buffer salts
    https://www.bioz.com/result/anti hamster igg whole molecule goat affinity purified antibody/product/Valiant
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hamster igg whole molecule goat affinity purified antibody - by Bioz Stars, 2021-04
    95/100 stars

    Images

    1) Product Images from "PPP2R2D suppresses IL-2 production and Treg function"

    Article Title: PPP2R2D suppresses IL-2 production and Treg function

    Journal: JCI Insight

    doi: 10.1172/jci.insight.138215

    PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of CD3 + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA IgG level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P
    Figure Legend Snippet: PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of CD3 + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA IgG level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P

    Techniques Used: Mouse Assay, FACS, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining

    2) Product Images from "Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex"

    Article Title: Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex

    Journal: Nature immunology

    doi: 10.1038/ni.3714

    GATA-3 and CTLA-4 are differentially sensitive to graded expression of BATF and IRF4 in T H 2 cells following increasing strength of TCR stimulation. ( a ) Flow cytometry analyzing IRF4 and BATF expression on day 4 of primary activation in WT CD4 + T cells cultured under T H 2 conditions (anti-IFN-γ, anti-IL-12 and IL-4) with anti-CD28 and the indicated concentration of anti-CD3ε crosslinked by plate-bound anti-hamster IgG. Numbers indicate the percentage of live CD4 + cells in each quadrant. ( b ) Flow cytometry analyzing GATA-3 and CTLA-4 expression on day 4 in WT CD4 + T cells cultured as in ( a ). ( c ) Overlays of flow cytometry data from ( a ) and ( b ) showing expression of the indicated proteins at various doses of plate bound anti-CD3ε. Data are representative of two experiments using five biological replicates ( a – c ).
    Figure Legend Snippet: GATA-3 and CTLA-4 are differentially sensitive to graded expression of BATF and IRF4 in T H 2 cells following increasing strength of TCR stimulation. ( a ) Flow cytometry analyzing IRF4 and BATF expression on day 4 of primary activation in WT CD4 + T cells cultured under T H 2 conditions (anti-IFN-γ, anti-IL-12 and IL-4) with anti-CD28 and the indicated concentration of anti-CD3ε crosslinked by plate-bound anti-hamster IgG. Numbers indicate the percentage of live CD4 + cells in each quadrant. ( b ) Flow cytometry analyzing GATA-3 and CTLA-4 expression on day 4 in WT CD4 + T cells cultured as in ( a ). ( c ) Overlays of flow cytometry data from ( a ) and ( b ) showing expression of the indicated proteins at various doses of plate bound anti-CD3ε. Data are representative of two experiments using five biological replicates ( a – c ).

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Activation Assay, Cell Culture, Concentration Assay

    3) Product Images from "Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex"

    Article Title: Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex

    Journal: Nature immunology

    doi: 10.1038/ni.3714

    GATA-3 and CTLA-4 are differentially sensitive to graded expression of BATF and IRF4 in T H 2 cells following increasing strength of TCR stimulation. ( a ) Flow cytometry analyzing IRF4 and BATF expression on day 4 of primary activation in WT CD4 + T cells cultured under T H 2 conditions (anti-IFN-γ, anti-IL-12 and IL-4) with anti-CD28 and the indicated concentration of anti-CD3ε crosslinked by plate-bound anti-hamster IgG. Numbers indicate the percentage of live CD4 + cells in each quadrant. ( b ) Flow cytometry analyzing GATA-3 and CTLA-4 expression on day 4 in WT CD4 + T cells cultured as in ( a ). ( c ) Overlays of flow cytometry data from ( a ) and ( b ) showing expression of the indicated proteins at various doses of plate bound anti-CD3ε. Data are representative of two experiments using five biological replicates ( a – c ).
    Figure Legend Snippet: GATA-3 and CTLA-4 are differentially sensitive to graded expression of BATF and IRF4 in T H 2 cells following increasing strength of TCR stimulation. ( a ) Flow cytometry analyzing IRF4 and BATF expression on day 4 of primary activation in WT CD4 + T cells cultured under T H 2 conditions (anti-IFN-γ, anti-IL-12 and IL-4) with anti-CD28 and the indicated concentration of anti-CD3ε crosslinked by plate-bound anti-hamster IgG. Numbers indicate the percentage of live CD4 + cells in each quadrant. ( b ) Flow cytometry analyzing GATA-3 and CTLA-4 expression on day 4 in WT CD4 + T cells cultured as in ( a ). ( c ) Overlays of flow cytometry data from ( a ) and ( b ) showing expression of the indicated proteins at various doses of plate bound anti-CD3ε. Data are representative of two experiments using five biological replicates ( a – c ).

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Activation Assay, Cell Culture, Concentration Assay

    Related Articles

    Purification:

    Article Title: Single-cell RNA-Seq analysis identifies a noncoding interleukin 4 (IL-4) RNA that post-transcriptionally up-regulates IL-4 production in T helper cells
    Article Snippet: CD4+ T cells were purified from the spleens of C57BL/6 mice using a magnetic biotin selection kit (Stem Cell). .. Purified CD4 T cells were activated with plate-bound anti-hamster IgG (MP Biomedicals) and soluble anti-CD3 (5 μg/ml, 145-2C11, Bio X Cell) and anti-CD28 (1 μg/ml, 37N, Bio X Cell) in T cell medium (Iscove's modified Dulbecco's medium, 10% FBS, 1× glutamine, 1× penicillin, 1× streptomycin, and 50 μ m β-mercaptoethanol), and defined as the TH 0 condition. .. For other subsets, various combinations of antibodies and cytokines were added as follows: TH 1 conditions, anti-IL-4 (5 μg/ml, 11B11, Bio X Cell) and IL-12 (2 ng/ml, PeproTech); TH 2 conditions, anti-IFN-γ (5 μg/ml, Bio X Cell) and IL-4 (10 ng/ml, PeproTech); TH 17 conditions, transforming growth factor β1(1 ng/ml, PeproTech), IL-6 (20 ng/ml, PeproTech), anti-IL-4 (5 μg/ml), and anti-IFN-γ (5 μg/ml); iTreg conditions, IL-2 (100 units/ml, PeproTech), TGF-β1 (5 ng/ml), anti-IL-4 (5 μg/ml), and anti-IFN-γ (5 μg/ml).

    Article Title: PPP2R2D suppresses IL-2 production and Treg function
    Article Snippet: Naive CD4+ T cells were purified by mouse CD4+ CD62L+ T Cell Isolation Kit II (Miltenyi Biotec). .. Purified naive T cells were stimulated with plate-bound goat anti–hamster IgG (SKU 0856984, MP Biomedicals), soluble anti-CD3 (0.25 μg/mL,145-2C11; BioLegend), and anti-CD28 (0.5 μg/mL, 37.51; BioLegend) for Th0-nonpolarized condition culture. .. In addition to Th0-nonpolarized condition, the following stimulation was used for each polarized condition: IL-12 (20 ng/mL; R & D Systems) and anti–IL-4 (10 μg/mL, C17.8; BioLegend) for Th1; IL-6 (3 ng/mL; R & D Systems), TGF-β1 (0.3 ng/mL; R & D Systems), anti–IL-4 (10 μg/mL, C17.8; BioLegend), and anti–IFN-γ (10 μg/mL; XMG1.2; BioLegend) for Th17; and IL-2 (20 ng/mL; R & D Systems), TGF-β1 (3 ng/mL), anti–IL-4 (10 μg/mL), and anti–IFN-γ (10 μg/mL) for Treg.

    Modification:

    Article Title: Single-cell RNA-Seq analysis identifies a noncoding interleukin 4 (IL-4) RNA that post-transcriptionally up-regulates IL-4 production in T helper cells
    Article Snippet: CD4+ T cells were purified from the spleens of C57BL/6 mice using a magnetic biotin selection kit (Stem Cell). .. Purified CD4 T cells were activated with plate-bound anti-hamster IgG (MP Biomedicals) and soluble anti-CD3 (5 μg/ml, 145-2C11, Bio X Cell) and anti-CD28 (1 μg/ml, 37N, Bio X Cell) in T cell medium (Iscove's modified Dulbecco's medium, 10% FBS, 1× glutamine, 1× penicillin, 1× streptomycin, and 50 μ m β-mercaptoethanol), and defined as the TH 0 condition. .. For other subsets, various combinations of antibodies and cytokines were added as follows: TH 1 conditions, anti-IL-4 (5 μg/ml, 11B11, Bio X Cell) and IL-12 (2 ng/ml, PeproTech); TH 2 conditions, anti-IFN-γ (5 μg/ml, Bio X Cell) and IL-4 (10 ng/ml, PeproTech); TH 17 conditions, transforming growth factor β1(1 ng/ml, PeproTech), IL-6 (20 ng/ml, PeproTech), anti-IL-4 (5 μg/ml), and anti-IFN-γ (5 μg/ml); iTreg conditions, IL-2 (100 units/ml, PeproTech), TGF-β1 (5 ng/ml), anti-IL-4 (5 μg/ml), and anti-IFN-γ (5 μg/ml).

    In Vitro:

    Article Title: T-cell specific deletion of STIM1 and STIM2 protects mice from EAE by impairing the effector functions of Th1 and Th17 cells
    Article Snippet: For cytokine measurements, CD4+ T cells isolated from spleen and lymph nodes of MOG immunized mice were stimulated with 50 μg/ml MOG35-55 either together with irradiated (20 Gy) splenocytes from syngeneic C57BL/6 mice or 10 ng/ml IL-23 as indicated. .. For in vitro differentiation of CD4+ T cells into ThN, Th1 or Th17 cells, 1x106 negatively selected CD4+ T cells were stimulated with 0.25 μg/ml anti-CD3 (145-2C11, eBioscience) and 1 μg/ml anti-CD28 (37.51, eBioscience) in goat-anti-hamster IgG (0.12 mg/ml, MP Biomedicals) pre-coated 6-well plates. .. For ThN cells, no further supplements were added; for Th1 cells, 10 ng/ml IL-12 (Peprotech) and 5 μg/ml anti-IL-4 (11B11, eBioscience) were added; for Th17 cells, 10 μg/ml anti-IFN-γ (XMG1.2, eBioscience), 10 μg/ml anti-IL-4, 20 ng/ml mouse IL-6 (Peprotech, Rocky Hill, NJ) and 0.5 ng/ml human TGF-β1 (Peprotech) were added.

    Activation Assay:

    Article Title: Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex
    Article Snippet: For time course and TCR dose titration experiments (FACS, microarray and ChIP) naïve CD4+ T cells were sorted as CD4+ CD25− CD44− cells using spleen and lymph node cells after negative selection with biotinylated antibodies against B220, DX5 and CD8, streptavidin-nanobeads and MojoSort Magnetic Cell Separation system (Biolegend). .. Naïve CD4+ T cells were activated with soluble anti-CD3ε (145-2C11, 1 μg/ml, BioXCell) at various concentrations cross linked by plate-bound anti-hamster IgG (MP biomedicals, 1:50) and anti-CD28 (37.51, BioXCell) (4 μg/ml) under TH 2 conditions (anti-IFN-γ 10 μg/ml (XMG1.2, BioXCell), anti-IL-12 10 μg/ml (Tosh), IL-4 10 ng/ml (Peprotech)) and were analyzed on the indicated day after primary activation. .. For peptide dose titration, naïve KJ126+ cells from DO11.10 mice were purified and activated with MACS purified CD11c+ dendritic cells from spleens of BALB/c mice in the presence of ovalbumin (323–339) peptide under TH 2 conditions.

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  • 95
    Valiant anti hamster igg whole molecule goat affinity purified antibody
    PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of <t>CD3</t> + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA <t>IgG</t> level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P
    Anti Hamster Igg Whole Molecule Goat Affinity Purified Antibody, supplied by Valiant, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hamster igg whole molecule goat affinity purified antibody/product/Valiant
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hamster igg whole molecule goat affinity purified antibody - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of CD3 + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA IgG level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P

    Journal: JCI Insight

    Article Title: PPP2R2D suppresses IL-2 production and Treg function

    doi: 10.1172/jci.insight.138215

    Figure Lengend Snippet: PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of CD3 + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA IgG level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P

    Article Snippet: Purified naive T cells were stimulated with plate-bound goat anti–hamster IgG (SKU 0856984, MP Biomedicals), soluble anti-CD3 (0.25 μg/mL,145-2C11; BioLegend), and anti-CD28 (0.5 μg/mL, 37.51; BioLegend) for Th0-nonpolarized condition culture.

    Techniques: Mouse Assay, FACS, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining

    GATA-3 and CTLA-4 are differentially sensitive to graded expression of BATF and IRF4 in T H 2 cells following increasing strength of TCR stimulation. ( a ) Flow cytometry analyzing IRF4 and BATF expression on day 4 of primary activation in WT CD4 + T cells cultured under T H 2 conditions (anti-IFN-γ, anti-IL-12 and IL-4) with anti-CD28 and the indicated concentration of anti-CD3ε crosslinked by plate-bound anti-hamster IgG. Numbers indicate the percentage of live CD4 + cells in each quadrant. ( b ) Flow cytometry analyzing GATA-3 and CTLA-4 expression on day 4 in WT CD4 + T cells cultured as in ( a ). ( c ) Overlays of flow cytometry data from ( a ) and ( b ) showing expression of the indicated proteins at various doses of plate bound anti-CD3ε. Data are representative of two experiments using five biological replicates ( a – c ).

    Journal: Nature immunology

    Article Title: Quality of TCR signaling encoded by differential enhancer affinities for the composite BATF-IRF4 transcription factor complex

    doi: 10.1038/ni.3714

    Figure Lengend Snippet: GATA-3 and CTLA-4 are differentially sensitive to graded expression of BATF and IRF4 in T H 2 cells following increasing strength of TCR stimulation. ( a ) Flow cytometry analyzing IRF4 and BATF expression on day 4 of primary activation in WT CD4 + T cells cultured under T H 2 conditions (anti-IFN-γ, anti-IL-12 and IL-4) with anti-CD28 and the indicated concentration of anti-CD3ε crosslinked by plate-bound anti-hamster IgG. Numbers indicate the percentage of live CD4 + cells in each quadrant. ( b ) Flow cytometry analyzing GATA-3 and CTLA-4 expression on day 4 in WT CD4 + T cells cultured as in ( a ). ( c ) Overlays of flow cytometry data from ( a ) and ( b ) showing expression of the indicated proteins at various doses of plate bound anti-CD3ε. Data are representative of two experiments using five biological replicates ( a – c ).

    Article Snippet: On day 7, cells were re-stimulated under the same conditions or were re-stimulated using soluble anti-CD3ε (145-2C11, 1 μg/ml, BioXCell) at various concentrations cross linked by plate-bound anti-hamster IgG (MP biomedicals, 1:50) and anti-CD28 (37.51, BioXCell) (4 μg/ml) under TH 2 conditions.

    Techniques: Expressing, Flow Cytometry, Cytometry, Activation Assay, Cell Culture, Concentration Assay