igg1  (Valiant)

 
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    Name:
    IgG rabbit purified IgG
    Description:
    Product is the lyophilized powder of rabbit IgG and buffer salts Rabbit IgG is purified from pooled rabbit serum using multi step procedures which may include salt fractionation gel filtration ion exchange chromatography and immunoabsorption The product is dialyzed into 0 01M sodium phosphate 0 07M sodium chloride pH 7 3 filtered through a 0 22 µm filter vialed and lyophilized No preservative added
    Catalog Number:
    0855944
    Price:
    216.5
    Category:
    Life Sciences Biochemicals Proteins and Derivatives Tissue and Blood Products
    Applications:
    Immunoassays, ELISA, Dot immunobinding, Immunoblot, Immunodiffusion, Immunoelectrophoresis
    Size:
    50 mg
    Buy from Supplier


    Structured Review

    Valiant igg1
    IgG rabbit purified IgG
    Product is the lyophilized powder of rabbit IgG and buffer salts Rabbit IgG is purified from pooled rabbit serum using multi step procedures which may include salt fractionation gel filtration ion exchange chromatography and immunoabsorption The product is dialyzed into 0 01M sodium phosphate 0 07M sodium chloride pH 7 3 filtered through a 0 22 µm filter vialed and lyophilized No preservative added
    https://www.bioz.com/result/igg1/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg1 - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity"

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063269

    Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P
    Figure Legend Snippet: Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    Related Articles

    Purification:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Article Title: Purification of specific loci for proteomic analysis
    Article Snippet: Dynabeads M270-epoxy (Invitrogen 143-02D) are suspended in N,N-dimethylformamide (Fisher D119) at 30 mg/mL and stored at 4°C. .. Purified rabbit IgG (MP Biomedicals 55944) is re-suspended at 17 mg/mL in dH2 O and stored at −80°C in 100 μL aliquots. ..

    Article Title: Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection
    Article Snippet: Immunoaffinity purification sample preparation MRC5 cells were mechanically lysed with a Polytron in lysis buffer (20 mM HEPES-KOH pH 7.4, 110 mM potassium acetate, 2 mM MgCl2 , 0.1% Tween-20, 1 μM ZnCl2 , and 1 μM CaCl2 ) containing 0.2% Triton X-100 and protease and phosphatase inhibitor cocktail. .. Protein A/G magnetic beads (Pierce #88802) were washed with the lysis buffer and conjugated to the following antibodies for 1 h at 4 °C; anti-FLAG antibody (AB_262044), anti-IFIT1 antibody (AB_2783869), or purified rabbit IgG (MP Biomedicals). .. Cell lysates were incubated with the conjugated beads for 1 h at 4 °C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Lysis:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Article Title: Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection
    Article Snippet: Immunoaffinity purification sample preparation MRC5 cells were mechanically lysed with a Polytron in lysis buffer (20 mM HEPES-KOH pH 7.4, 110 mM potassium acetate, 2 mM MgCl2 , 0.1% Tween-20, 1 μM ZnCl2 , and 1 μM CaCl2 ) containing 0.2% Triton X-100 and protease and phosphatase inhibitor cocktail. .. Protein A/G magnetic beads (Pierce #88802) were washed with the lysis buffer and conjugated to the following antibodies for 1 h at 4 °C; anti-FLAG antibody (AB_262044), anti-IFIT1 antibody (AB_2783869), or purified rabbit IgG (MP Biomedicals). .. Cell lysates were incubated with the conjugated beads for 1 h at 4 °C.

    Labeling:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Protease Inhibitor:

    Article Title: Integrative Structure and Functional Anatomy of a Nuclear Pore Complex
    Article Snippet: The diploid Saccharomyces uvarum strain (ATCC 9080) was grown and processed for NE purification as previously described . .. The following materials were used in this study: Dynabeads M-270 Epoxy (143.02D; Invitrogen); rabbit IgG (55944; MP Biomedicals); protease inhibitor cocktail (P-8340; Sigma-Aldrich); and Solution P (2 mg Pepstatin A, 90 mg PMSF, and 5 mL of absolute ethanol). .. Immuno-purification of the endogenous S. cerevisiae NPC A novel immuno-purification protocol for the isolation of endogenous whole NPCs from S. cerevisiae was developed using previously published methodology – .

    Staining:

    Article Title: Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells
    Article Snippet: After deparaffinization with xylene and rehydration, the slides were blocked by pre-incubation with 10% normal goat serum (KPL, 710027) for 1 h. Paraformaldehyde-fixed VSMCs were permeabilized by incubation with 0.1% Triton X-100 in phosphate-buffered saline (PBS) for 30 min. Then, the tissue section or cells were incubated overnight at 4°C with the following primary antibodies: anti-α-SMA (1:50, Santa Cruz Biotechnology, sc-130617), anti-Beclin1 (1:100, Abcam, ab207612), anti-LC3B (1:100, Abcam, ab48394), anti-KLF3 (1:50, Santa Cruz Biotechnology, sc-514500), anti-NEDD4L (1:100, Abcam, ab46521), and anti-PFKP (1:100, Abcam, ab204131). .. Secondary antibodies were rhodamine-labeled antibody to rabbit immunoglobulin G (IgG) (1:50, KPL, 031506) and fluorescein-labeled antibody to mouse IgG (1:50, KPL, 021815), or rhodamine-labeled antibody to mouse IgG (1:50, KPL, 031806) and fluorescein-labeled antibody to rabbit IgG (1:50, KPL, 021516), and 4′,6-diamidino-2-phenylindole (DAPI) (1:50, MP Biomedicals, 157574) was used to stain nuclei in each experiment. .. Images were captured by confocal microscopy (DM6000 CFS, Leica Microsystems) and processed by LAS AF software.

    Incubation:

    Article Title: Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions
    Article Snippet: The soluble fraction was isolated by centrifugation for 10 minutes; the supernatant was decanted and subjected to another 10 minute centrifugation step. .. The supernatant was incubated with 25 mg of Dynabeads (Invitrogen) cross-linked to rabbit IgG (MP Biomedicals) with gentle agitation for 10 minutes. ..

    Magnetic Beads:

    Article Title: Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection
    Article Snippet: Immunoaffinity purification sample preparation MRC5 cells were mechanically lysed with a Polytron in lysis buffer (20 mM HEPES-KOH pH 7.4, 110 mM potassium acetate, 2 mM MgCl2 , 0.1% Tween-20, 1 μM ZnCl2 , and 1 μM CaCl2 ) containing 0.2% Triton X-100 and protease and phosphatase inhibitor cocktail. .. Protein A/G magnetic beads (Pierce #88802) were washed with the lysis buffer and conjugated to the following antibodies for 1 h at 4 °C; anti-FLAG antibody (AB_262044), anti-IFIT1 antibody (AB_2783869), or purified rabbit IgG (MP Biomedicals). .. Cell lysates were incubated with the conjugated beads for 1 h at 4 °C.

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  • 93
    Valiant rabbit igg
    Thermal profiling points to virus–virus and virus–host protein interactions, including the association of the essential HCMV pUL52 with immunomodulatory host proteins. a Distance matrix of Ex for pairs of quantified viral proteins. Color indicates Ex value for the corresponding protein pair. Clusters #1 and 2 highlight potential viral complexes. b Coaggregation curves of IE1 and IE2 (left), MCP, SCP, and RIR1 (left middle), proteins in cluster #1 (right middle) and proteins in cluster #2 (right) at 96 hpi. Known and literature-suggested complex components are indicated in red. c , d Coaggregation curves of c pUL38- TSC1-TSC2 and d <t>pUL52-IFIT1-</t> IFIT2 at each infection time point. pUL52 was not detected at mock and 24 hpi. Statistical analysis of curve similarities is shown with p -values (corrected with Benjamini-Hochberg procedure generated using random protein pair sampling). e IP-PRM analysis validating the complex composed of pUL52, IFIT1, and IFIT2. An anti-IFIT1 IP or a control <t>IgG</t> IP was conducted at 48 and 96 hpi and abundances of pUL52, IFIT1, and IFIT2 were quantified by PRM. Data represent average normalized abundance ± SD, n = 1 for 48 hpi, and n = 2 for 96 hpi.
    Rabbit Igg, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit igg/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit igg - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Thermal profiling points to virus–virus and virus–host protein interactions, including the association of the essential HCMV pUL52 with immunomodulatory host proteins. a Distance matrix of Ex for pairs of quantified viral proteins. Color indicates Ex value for the corresponding protein pair. Clusters #1 and 2 highlight potential viral complexes. b Coaggregation curves of IE1 and IE2 (left), MCP, SCP, and RIR1 (left middle), proteins in cluster #1 (right middle) and proteins in cluster #2 (right) at 96 hpi. Known and literature-suggested complex components are indicated in red. c , d Coaggregation curves of c pUL38- TSC1-TSC2 and d pUL52-IFIT1- IFIT2 at each infection time point. pUL52 was not detected at mock and 24 hpi. Statistical analysis of curve similarities is shown with p -values (corrected with Benjamini-Hochberg procedure generated using random protein pair sampling). e IP-PRM analysis validating the complex composed of pUL52, IFIT1, and IFIT2. An anti-IFIT1 IP or a control IgG IP was conducted at 48 and 96 hpi and abundances of pUL52, IFIT1, and IFIT2 were quantified by PRM. Data represent average normalized abundance ± SD, n = 1 for 48 hpi, and n = 2 for 96 hpi.

    Journal: Nature Communications

    Article Title: Temporal dynamics of protein complex formation and dissociation during human cytomegalovirus infection

    doi: 10.1038/s41467-020-14586-5

    Figure Lengend Snippet: Thermal profiling points to virus–virus and virus–host protein interactions, including the association of the essential HCMV pUL52 with immunomodulatory host proteins. a Distance matrix of Ex for pairs of quantified viral proteins. Color indicates Ex value for the corresponding protein pair. Clusters #1 and 2 highlight potential viral complexes. b Coaggregation curves of IE1 and IE2 (left), MCP, SCP, and RIR1 (left middle), proteins in cluster #1 (right middle) and proteins in cluster #2 (right) at 96 hpi. Known and literature-suggested complex components are indicated in red. c , d Coaggregation curves of c pUL38- TSC1-TSC2 and d pUL52-IFIT1- IFIT2 at each infection time point. pUL52 was not detected at mock and 24 hpi. Statistical analysis of curve similarities is shown with p -values (corrected with Benjamini-Hochberg procedure generated using random protein pair sampling). e IP-PRM analysis validating the complex composed of pUL52, IFIT1, and IFIT2. An anti-IFIT1 IP or a control IgG IP was conducted at 48 and 96 hpi and abundances of pUL52, IFIT1, and IFIT2 were quantified by PRM. Data represent average normalized abundance ± SD, n = 1 for 48 hpi, and n = 2 for 96 hpi.

    Article Snippet: Protein A/G magnetic beads (Pierce #88802) were washed with the lysis buffer and conjugated to the following antibodies for 1 h at 4 °C; anti-FLAG antibody (AB_262044), anti-IFIT1 antibody (AB_2783869), or purified rabbit IgG (MP Biomedicals).

    Techniques: Infection, Generated, Sampling

    Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P

    Journal: PLoS ONE

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity

    doi: 10.1371/journal.pone.0063269

    Figure Lengend Snippet: Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P

    Article Snippet: Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification