peroxidase conjugated goat anti rabbit igg  (Valiant)

 
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    Name:
    Anti rabbit IgG F Ab 2 affinity purified goat antibody peroxidase conjugated
    Description:
    Product is horseradish peroxidase HRP conjugated goat affinity purified antibody to rabbit IgG F ab 2 and buffer salts
    Catalog Number:
    0855691
    Price:
    331.7
    Category:
    Life Sciences Antibodies Secondary Antibodies
    Applications:
    Immunohistochemistry, Immunoblotting ,ELISA
    Size:
    2 mL
    Buy from Supplier


    Structured Review

    Valiant peroxidase conjugated goat anti rabbit igg
    Physical association and co-localization between <t>Mfa1</t> and Mfa2. Mfa1 or Mfa2 was immunoprecipitated from whole-cell lysate of 33277 with anti-Mfa1 or anti-Mfa2 serum. The precipitates were separated by SDS-PAGE (a), followed by Western blotting using either anti-Mfa1 (b) or anti-Mfa2 serum (c). Lanes: 1, 33277 precipitate with anti-Mfa1; 2, 33277 precipitate with anti-Mfa2; 3, 33277 precipitate with anti-OmpA (Pgm6/7) as a negative control. The positions of Mfa1, Mfa2 and molecular mass markers are indicated. (d) Single-labelled, immunogold electron microscopy. Ultra-thin sections of JI-1 (Δ fimA ) were reacted with chicken anti-Mfa1, followed by 6 nm gold-labelled anti-chicken Ig. Bar, 100 nm. (e) Double-labelling immunogold microscopy. The sections were first reacted with chicken anti-Mfa1 and rabbit anti-Mfa2 serum, followed by incubation with 6 nm gold-labelled anti-chicken Ig and 20 nm gold-labelled anti-rabbit <t>IgG.</t> Bar, 50 nm.
    Product is horseradish peroxidase HRP conjugated goat affinity purified antibody to rabbit IgG F ab 2 and buffer salts
    https://www.bioz.com/result/peroxidase conjugated goat anti rabbit igg/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti rabbit igg - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2"

    Article Title: Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2

    Journal: Microbiology

    doi: 10.1099/mic.0.028928-0

    Physical association and co-localization between Mfa1 and Mfa2. Mfa1 or Mfa2 was immunoprecipitated from whole-cell lysate of 33277 with anti-Mfa1 or anti-Mfa2 serum. The precipitates were separated by SDS-PAGE (a), followed by Western blotting using either anti-Mfa1 (b) or anti-Mfa2 serum (c). Lanes: 1, 33277 precipitate with anti-Mfa1; 2, 33277 precipitate with anti-Mfa2; 3, 33277 precipitate with anti-OmpA (Pgm6/7) as a negative control. The positions of Mfa1, Mfa2 and molecular mass markers are indicated. (d) Single-labelled, immunogold electron microscopy. Ultra-thin sections of JI-1 (Δ fimA ) were reacted with chicken anti-Mfa1, followed by 6 nm gold-labelled anti-chicken Ig. Bar, 100 nm. (e) Double-labelling immunogold microscopy. The sections were first reacted with chicken anti-Mfa1 and rabbit anti-Mfa2 serum, followed by incubation with 6 nm gold-labelled anti-chicken Ig and 20 nm gold-labelled anti-rabbit IgG. Bar, 50 nm.
    Figure Legend Snippet: Physical association and co-localization between Mfa1 and Mfa2. Mfa1 or Mfa2 was immunoprecipitated from whole-cell lysate of 33277 with anti-Mfa1 or anti-Mfa2 serum. The precipitates were separated by SDS-PAGE (a), followed by Western blotting using either anti-Mfa1 (b) or anti-Mfa2 serum (c). Lanes: 1, 33277 precipitate with anti-Mfa1; 2, 33277 precipitate with anti-Mfa2; 3, 33277 precipitate with anti-OmpA (Pgm6/7) as a negative control. The positions of Mfa1, Mfa2 and molecular mass markers are indicated. (d) Single-labelled, immunogold electron microscopy. Ultra-thin sections of JI-1 (Δ fimA ) were reacted with chicken anti-Mfa1, followed by 6 nm gold-labelled anti-chicken Ig. Bar, 100 nm. (e) Double-labelling immunogold microscopy. The sections were first reacted with chicken anti-Mfa1 and rabbit anti-Mfa2 serum, followed by incubation with 6 nm gold-labelled anti-chicken Ig and 20 nm gold-labelled anti-rabbit IgG. Bar, 50 nm.

    Techniques Used: Immunoprecipitation, SDS Page, Western Blot, Negative Control, Electron Microscopy, Microscopy, Incubation

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    Incubation:

    Article Title: PCP4/PEP19 promotes migration, invasion and adhesion in human breast cancer MCF-7 and T47D cells
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies diluted in Can Get Signal solution 1 (Toyobo, Osaka, Japan), followed by horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse antibody (MP Biomedicals, Santa Ana, CA). .. Protein expression was detected with SuperSignal West Pico chemiluminescent substrate or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific, Waltham, MA).

    Article Title: Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice
    Article Snippet: The proteins separated by SDS–PAGE were transferred onto Immuno-Blot™ PVDF membrane (Bio-Rad, http://www.bio-rad.com/ ) by electroblotting (Transblot SD, Bio-Rad). .. After treatment with blocking solution containing 5% skim milk, the membrane was incubated with anti-CEBiP antiserum at a final dilution of 1:2500 or anti-myc antibody at a final dilution of 1:2000, followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit IgG (MP Biomedicals, http://www.mpbio.com/ ). .. Chemiluminescent detection was performed using Immobilon™ Western reagent kit (Millipore).

    Imaging:

    Article Title: Non-acylated Wnts can promote signaling
    Article Snippet: Successful production and secretion of Wnts was determined by Western blot. .. Conditioned supernatant was analyzed by immunoblotting with the above-mentioned antibodies for xWnt8 and Wnt1 (1:1000) and peroxidase-conjugated goat anti-rabbit IgG secondary antibody (MP Biomedicals 55689), with detection by chemiluminescence using SuperSignal West Pico substrate (ThermoFisher), detected with a LI-COR Odyssey Fc Imaging System. .. For analyzing β-catenin dependent signaling in vitro we used a previously described luciferase reporter cell-line ( ) comprising HEK 293 cells stably transfected with a plasmid containing 7 x LEF/TCF sites and a minimal thymidine kinase promoter that drives expression of firefly luciferase (HEK 293 STF).

    Blocking Assay:

    Article Title: Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice
    Article Snippet: The proteins separated by SDS–PAGE were transferred onto Immuno-Blot™ PVDF membrane (Bio-Rad, http://www.bio-rad.com/ ) by electroblotting (Transblot SD, Bio-Rad). .. After treatment with blocking solution containing 5% skim milk, the membrane was incubated with anti-CEBiP antiserum at a final dilution of 1:2500 or anti-myc antibody at a final dilution of 1:2000, followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit IgG (MP Biomedicals, http://www.mpbio.com/ ). .. Chemiluminescent detection was performed using Immobilon™ Western reagent kit (Millipore).

    Article Title: Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish: Implication for the robust innate defense mechanisms of teleosts
    Article Snippet: For Western blotting, the separated proteins were electroblotted onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare Life Sciences, Little Chalfont, UK) in a transfer buffer composed of 100 mM Tris, 192 mM glycine, 20% methanol, and 0.02% SDS. .. After blocking with 5% skim milk in PBS (SM-PBS), the membrane was treated with anti-ayu (Plecoglossus altivelis ) IgM rabbit serum (a gift from Dr. Masakazu Kondo, National Fisheries University, Shimonoseki, Japan; 1/200 diluted in SM-PBS) and then with peroxidase-conjugated goat anti-rabbit IgG (diluted 1/2,000 in SM-PBS; Cappel, MP Biomedicals, Santa Ana, CA, USA). .. This primary antibody was selected from three candidates of anti-teleost IgM antibodies by Western blotting (data not shown).

    Western Blot:

    Article Title: Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2
    Article Snippet: Proteins were separated with a 12 % (w/v) SDS-PAGE gel and visualized by staining with CBB. .. For Western immunoblotting, proteins were transferred onto a nitrocellulose membrane (Hybond ECL nitrocellulose membrane, GE Healthcare Bio-science) and detected by using antibody raised against Mfa1 ( ) or Mfa2 and peroxidase-conjugated goat anti-rabbit IgG (MP Biomedicals). ..

    Luciferase:

    Article Title: Ethacrynic and alpha-lipoic acids inhibit vaccinia virus late gene expression.
    Article Snippet: VACV polypeptides were detected with a rabbit antiserum against VACV (dilution 1:500; antiserum raised by immunization with a live VACV, generated and kindly provided by Drs. D. and J.R. Rodriguez ( Fig. 4A ; (Rodriguez et al., 1992) , or with an inactivated purified VACV, prepared by Seva-Imuno Praha, Czech Republic ( Fig. 4B) ), peroxidase-conjugated goat anti-rabbit IgG (dilutions 1:1000; MP Biomedicals -Cappel, Solon, OH) and a chromogenic substrate ␣chloronaphtol. .. Luciferase was detected with a rabbit polyclonal antibody (dilution 1:500; Santa Cruz Biotechnology, Santa Cruz, CA), peroxidase-conjugated goat anti-rabbit IgG (dilution 1:20,000; MP Biomedicals -Cappel, Solon, OH) and chemiluminescence (West Femto, Thermo Fisher Scientific-Pierce, Rockford, IL). .. DNA isolation and quantification The cells were collected by pipetting, washed twice with PBS and total DNA was isolated using a treatment with proteinase K and a phenol-chloroform extraction (EA samples; (Ausubel et al., 2002) or using a PCR lysis buffer containing proteinase K (LA samples; (Schmidtmayerova et al., 1998) .

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  • 93
    Valiant peroxidase conjugated goat anti rabbit igg
    Physical association and co-localization between <t>Mfa1</t> and Mfa2. Mfa1 or Mfa2 was immunoprecipitated from whole-cell lysate of 33277 with anti-Mfa1 or anti-Mfa2 serum. The precipitates were separated by SDS-PAGE (a), followed by Western blotting using either anti-Mfa1 (b) or anti-Mfa2 serum (c). Lanes: 1, 33277 precipitate with anti-Mfa1; 2, 33277 precipitate with anti-Mfa2; 3, 33277 precipitate with anti-OmpA (Pgm6/7) as a negative control. The positions of Mfa1, Mfa2 and molecular mass markers are indicated. (d) Single-labelled, immunogold electron microscopy. Ultra-thin sections of JI-1 (Δ fimA ) were reacted with chicken anti-Mfa1, followed by 6 nm gold-labelled anti-chicken Ig. Bar, 100 nm. (e) Double-labelling immunogold microscopy. The sections were first reacted with chicken anti-Mfa1 and rabbit anti-Mfa2 serum, followed by incubation with 6 nm gold-labelled anti-chicken Ig and 20 nm gold-labelled anti-rabbit <t>IgG.</t> Bar, 50 nm.
    Peroxidase Conjugated Goat Anti Rabbit Igg, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti rabbit igg/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti rabbit igg - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Physical association and co-localization between Mfa1 and Mfa2. Mfa1 or Mfa2 was immunoprecipitated from whole-cell lysate of 33277 with anti-Mfa1 or anti-Mfa2 serum. The precipitates were separated by SDS-PAGE (a), followed by Western blotting using either anti-Mfa1 (b) or anti-Mfa2 serum (c). Lanes: 1, 33277 precipitate with anti-Mfa1; 2, 33277 precipitate with anti-Mfa2; 3, 33277 precipitate with anti-OmpA (Pgm6/7) as a negative control. The positions of Mfa1, Mfa2 and molecular mass markers are indicated. (d) Single-labelled, immunogold electron microscopy. Ultra-thin sections of JI-1 (Δ fimA ) were reacted with chicken anti-Mfa1, followed by 6 nm gold-labelled anti-chicken Ig. Bar, 100 nm. (e) Double-labelling immunogold microscopy. The sections were first reacted with chicken anti-Mfa1 and rabbit anti-Mfa2 serum, followed by incubation with 6 nm gold-labelled anti-chicken Ig and 20 nm gold-labelled anti-rabbit IgG. Bar, 50 nm.

    Journal: Microbiology

    Article Title: Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2

    doi: 10.1099/mic.0.028928-0

    Figure Lengend Snippet: Physical association and co-localization between Mfa1 and Mfa2. Mfa1 or Mfa2 was immunoprecipitated from whole-cell lysate of 33277 with anti-Mfa1 or anti-Mfa2 serum. The precipitates were separated by SDS-PAGE (a), followed by Western blotting using either anti-Mfa1 (b) or anti-Mfa2 serum (c). Lanes: 1, 33277 precipitate with anti-Mfa1; 2, 33277 precipitate with anti-Mfa2; 3, 33277 precipitate with anti-OmpA (Pgm6/7) as a negative control. The positions of Mfa1, Mfa2 and molecular mass markers are indicated. (d) Single-labelled, immunogold electron microscopy. Ultra-thin sections of JI-1 (Δ fimA ) were reacted with chicken anti-Mfa1, followed by 6 nm gold-labelled anti-chicken Ig. Bar, 100 nm. (e) Double-labelling immunogold microscopy. The sections were first reacted with chicken anti-Mfa1 and rabbit anti-Mfa2 serum, followed by incubation with 6 nm gold-labelled anti-chicken Ig and 20 nm gold-labelled anti-rabbit IgG. Bar, 50 nm.

    Article Snippet: For Western immunoblotting, proteins were transferred onto a nitrocellulose membrane (Hybond ECL nitrocellulose membrane, GE Healthcare Bio-science) and detected by using antibody raised against Mfa1 ( ) or Mfa2 and peroxidase-conjugated goat anti-rabbit IgG (MP Biomedicals).

    Techniques: Immunoprecipitation, SDS Page, Western Blot, Negative Control, Electron Microscopy, Microscopy, Incubation